In tomato, the NBARC-LRR resistance （R） protein Prf acts in concert with the Pto or Fen kinase to determine immunity against Pseudomonas syringae pv. tomato （Pst）. Prf-mediated defense signaling is initiated by th...In tomato, the NBARC-LRR resistance （R） protein Prf acts in concert with the Pto or Fen kinase to determine immunity against Pseudomonas syringae pv. tomato （Pst）. Prf-mediated defense signaling is initiated by the recognition of two sequence-unrelated Pst-secreted effector proteins, AvrPto and AvrPtoB, by tomato Pto or Fen. Prf detects these inter- actions and activates signaling leading to host defense responses including localized programmed cell death （PCD） that is associated with the arrest of Pst growth. We found that Prf variants with single amino acid substitutions at D1416 in the IHD motif （isoleucine-histidine-aspartic acid） in the NBARC domain cause effector-independent PCD when transiently expressed in leaves of Nicotiana benthamiana, suggesting D1416 plays an important role in activation of Prf. The N-ter- minal region of Prf （NPrf） and the LRR domain are required for this autoactive Prf cell death signaling but dispensable for accumulation of the PrfD1416V protein. Significantly, co-expression of the Prf LRR but not NPrf, with PrfD1416v, AvrPto/Pto, AvrPtoB/Pto, an autoactive form of Pro （PtoY207D）, or Fen completely suppresses PCD. However, the Prf LRR does not in- terfere with PCD caused by Rpi-blblD475v a distinct R protein-mediated PCD signaling event, or that caused by overex- pression of MAPKKKα, a protein acting downstream of Prf. Furthermore, we found the PrfD1416V protein is unable to accumulate in plant cells when co-expressed with the Prf LRR domain, likely explaining the cell death suppression. The mechanism for the LRR-induced degradation of PrfD1416V is unknown but may involve interference in the intramo- lecular interactions of Prf or to binding of the unattached LRR to other host proteins that are needed for Prf stability.展开更多
Activity 1.Think about the following questions and write down your answers before reading the text.1.Have you ever tried Liuzhou Luosifen?If so,what did you think of it?2.What makes Liuzhou Luosifen unique compared to...Activity 1.Think about the following questions and write down your answers before reading the text.1.Have you ever tried Liuzhou Luosifen?If so,what did you think of it?2.What makes Liuzhou Luosifen unique compared to other types of noodles?展开更多
Evidence available on the independent and combined associations of sleep duration,bedtime,and genetic predisposition with hearing loss was lacking.The present study included 15,827 participants from the Dongfeng–Tong...Evidence available on the independent and combined associations of sleep duration,bedtime,and genetic predisposition with hearing loss was lacking.The present study included 15,827 participants from the Dongfeng–Tongji cohort study.Genetic risk was characterized by polygenic risk score(PRS)based on 37 genetic loci related to hearing loss.We conducted multivariate logistic regression models to assess the odds ratio(OR)for hearing loss with sleep duration and bedtime,as well as the joint association and interaction with PRS.Results showed that hearing loss was independently associated with sleeping≥9 h/night compared to the recommended 7 to<8 h/night,and with bedtime≤9:00 p.m.and>9:00 p.m.to 10:00 p.m.compared to those with bedtime>10:00 p.m.to 11:00 p.m.,with estimated ORs of 1.25,1.27,and 1.16,respectively.展开更多
The resistance in tomato plants to bacterial speck caused by Pseudomonas syringae pv. tomato is triggered by the interactions between the plant resistance protein Pto and the pathogen avirulence proteins AvrPto or Avr...The resistance in tomato plants to bacterial speck caused by Pseudomonas syringae pv. tomato is triggered by the interactions between the plant resistance protein Pto and the pathogen avirulence proteins AvrPto or AvrPtoB. Fen is a gene encoding closely related functional protein kinases as the Pto gene. To investigate the status of resistance to the pathogen and natural variation of Pto and Fen genes in tomato, 67 lines including 29 growing in China were subject to disease resistance evaluation and fenthion-sensitivity test. Alleles of Pto and Fen were amplified from genomic DNA of 25 tomato lines using polymerase chain reaction （PCR） and sequences were determined by sequencing the PCR products. The results indicated that none of the 29 cultivars/hybrids growing in China were resistant to bacterial speck race 0 strain DC3000. Seven of eight tomato lines resistant to DC3000 were also fenthion-sensitive. Analysis of deduced amino acid sequences identified three novel residue substitutions between Pto and pto, and one new substitution identified between Fen and fen. A PCR-based marker was developed and successfully used to select plants with resistance to DC3000.展开更多
With the city development and construction, ecological corridor has become one of the most attractive areas in urban districts. Ecological corridor has produced not only ecological benefits, but also economic benefits...With the city development and construction, ecological corridor has become one of the most attractive areas in urban districts. Ecological corridor has produced not only ecological benefits, but also economic benefits and social benefits, especially banded ecological park. By analyzing the impact of Fen River Park on leisure space, living space, communication space, commercial space of Taiyuan, and expounding shortcomings of constructing Fen River Ecological Corridor, countermeasures and suggestions were put forward.展开更多
文章主要考察了宁化客家方言的三个被动标记“分[p n 44]”、“畀[pei 31]”、“得[t 5]”。描写了其在宁化客家方言中的基本用法,同时,探讨了宁化客家方言中被动句的基本类型、构成成分的主要特征以及被动句式的语义色彩,并探究了宁化...文章主要考察了宁化客家方言的三个被动标记“分[p n 44]”、“畀[pei 31]”、“得[t 5]”。描写了其在宁化客家方言中的基本用法,同时,探讨了宁化客家方言中被动句的基本类型、构成成分的主要特征以及被动句式的语义色彩,并探究了宁化客家方言被动标记的语法化来源和归纳其语法化路径。展开更多
Aswe embarked on our half-day escapadetotheenigmaticDafen Oil PaintingVillage,awhirlwindof emotions swirled within us.Excitement mingled witha tingeof skepticism;could this renowned art factory truly live up to the my...Aswe embarked on our half-day escapadetotheenigmaticDafen Oil PaintingVillage,awhirlwindof emotions swirled within us.Excitement mingled witha tingeof skepticism;could this renowned art factory truly live up to the mystical allure we had heard about?In the late1980s,anentrepreneurial trade painternamed Huang Jiangmade a daringmove,relocating his business from HongKong to the outskirts of Shenzhen.Little did he know that this decision would set Dafenona remarkable trajectory to become the world's art factory.展开更多
Germany and China use different stable systems for fattening pigs. Widespread systems in Germany are closed stables with slatted or partly slatted floor, which produce liquid manure （a mixture of urine, faeces, and c...Germany and China use different stable systems for fattening pigs. Widespread systems in Germany are closed stables with slatted or partly slatted floor, which produce liquid manure （a mixture of urine, faeces, and cleaning water） whereas in China open stables with closed concrete floors are used with separate collection of faeces and urine. Based on data from literature and own analyses nutrient flows of both systems are calculated. The Chinese manure system or mechanized belt or V-shaped pit floor systems with faeces/urine separation are more effective to separate solids, nutrients, carbon, and heavy metal than mechanical separation from liquid manure. The separation rates can be up to 88% for the solids, 67% for nitrogen, 93% for phosphorus, 60% for potassium, 94% for magnesium, 97% for cupper, and 98% for zinc. The faeces can be used for composting to produce a market product.展开更多
The dynamic process of the 2008 Wenehuan earthquake is simulated by finite-element method （ FEM）, and the results suggest that we may be able to estimate the occurrence time, location, and magnitude of similar earth...The dynamic process of the 2008 Wenehuan earthquake is simulated by finite-element method （ FEM）, and the results suggest that we may be able to estimate the occurrence time, location, and magnitude of similar earthquakes in the future. Thus a numerical earthquake prediction （NEP） program is proposed; the reliability of it is dependent on its gradual refinement and the parameters used in the models.展开更多
基金the University of Idaho Startup funding to F.X.,the Knowledge Innovation Program of Chinese Academy of Sciences (KSCX2-EW-J-22) to X.M.,the National Science Fund for Distinguished Young Scholars (No.30825030) to Y.L.,and the National Science Foundation grant (DBI-0605059) to G.B.M
文摘In tomato, the NBARC-LRR resistance （R） protein Prf acts in concert with the Pto or Fen kinase to determine immunity against Pseudomonas syringae pv. tomato （Pst）. Prf-mediated defense signaling is initiated by the recognition of two sequence-unrelated Pst-secreted effector proteins, AvrPto and AvrPtoB, by tomato Pto or Fen. Prf detects these inter- actions and activates signaling leading to host defense responses including localized programmed cell death （PCD） that is associated with the arrest of Pst growth. We found that Prf variants with single amino acid substitutions at D1416 in the IHD motif （isoleucine-histidine-aspartic acid） in the NBARC domain cause effector-independent PCD when transiently expressed in leaves of Nicotiana benthamiana, suggesting D1416 plays an important role in activation of Prf. The N-ter- minal region of Prf （NPrf） and the LRR domain are required for this autoactive Prf cell death signaling but dispensable for accumulation of the PrfD1416V protein. Significantly, co-expression of the Prf LRR but not NPrf, with PrfD1416v, AvrPto/Pto, AvrPtoB/Pto, an autoactive form of Pro （PtoY207D）, or Fen completely suppresses PCD. However, the Prf LRR does not in- terfere with PCD caused by Rpi-blblD475v a distinct R protein-mediated PCD signaling event, or that caused by overex- pression of MAPKKKα, a protein acting downstream of Prf. Furthermore, we found the PrfD1416V protein is unable to accumulate in plant cells when co-expressed with the Prf LRR domain, likely explaining the cell death suppression. The mechanism for the LRR-induced degradation of PrfD1416V is unknown but may involve interference in the intramo- lecular interactions of Prf or to binding of the unattached LRR to other host proteins that are needed for Prf stability.
文摘Activity 1.Think about the following questions and write down your answers before reading the text.1.Have you ever tried Liuzhou Luosifen?If so,what did you think of it?2.What makes Liuzhou Luosifen unique compared to other types of noodles?
基金the Key Research and Development Program of Hubei Province(2022BCA046)the National Natural Scientific Foundation of China(81973128).
文摘Evidence available on the independent and combined associations of sleep duration,bedtime,and genetic predisposition with hearing loss was lacking.The present study included 15,827 participants from the Dongfeng–Tongji cohort study.Genetic risk was characterized by polygenic risk score(PRS)based on 37 genetic loci related to hearing loss.We conducted multivariate logistic regression models to assess the odds ratio(OR)for hearing loss with sleep duration and bedtime,as well as the joint association and interaction with PRS.Results showed that hearing loss was independently associated with sleeping≥9 h/night compared to the recommended 7 to<8 h/night,and with bedtime≤9:00 p.m.and>9:00 p.m.to 10:00 p.m.compared to those with bedtime>10:00 p.m.to 11:00 p.m.,with estimated ORs of 1.25,1.27,and 1.16,respectively.
基金supported by the National High Technology Research and Development Program of China (2006AA10Z1A6)the Program for New Century Excellent Talents in University, China (NCET-08-0531)
文摘The resistance in tomato plants to bacterial speck caused by Pseudomonas syringae pv. tomato is triggered by the interactions between the plant resistance protein Pto and the pathogen avirulence proteins AvrPto or AvrPtoB. Fen is a gene encoding closely related functional protein kinases as the Pto gene. To investigate the status of resistance to the pathogen and natural variation of Pto and Fen genes in tomato, 67 lines including 29 growing in China were subject to disease resistance evaluation and fenthion-sensitivity test. Alleles of Pto and Fen were amplified from genomic DNA of 25 tomato lines using polymerase chain reaction （PCR） and sequences were determined by sequencing the PCR products. The results indicated that none of the 29 cultivars/hybrids growing in China were resistant to bacterial speck race 0 strain DC3000. Seven of eight tomato lines resistant to DC3000 were also fenthion-sensitive. Analysis of deduced amino acid sequences identified three novel residue substitutions between Pto and pto, and one new substitution identified between Fen and fen. A PCR-based marker was developed and successfully used to select plants with resistance to DC3000.
文摘With the city development and construction, ecological corridor has become one of the most attractive areas in urban districts. Ecological corridor has produced not only ecological benefits, but also economic benefits and social benefits, especially banded ecological park. By analyzing the impact of Fen River Park on leisure space, living space, communication space, commercial space of Taiyuan, and expounding shortcomings of constructing Fen River Ecological Corridor, countermeasures and suggestions were put forward.
文摘Aswe embarked on our half-day escapadetotheenigmaticDafen Oil PaintingVillage,awhirlwindof emotions swirled within us.Excitement mingled witha tingeof skepticism;could this renowned art factory truly live up to the mystical allure we had heard about?In the late1980s,anentrepreneurial trade painternamed Huang Jiangmade a daringmove,relocating his business from HongKong to the outskirts of Shenzhen.Little did he know that this decision would set Dafenona remarkable trajectory to become the world's art factory.
基金The authors would like to thank the German Ministry for Education and Research （BMBF） （project No. 0330847G）, the National Natural Science Foundation of China （project No. 40971177）, and the Ministry of Science and Technology of the People＇s Republic of China （MOST） for financing the project.
文摘Germany and China use different stable systems for fattening pigs. Widespread systems in Germany are closed stables with slatted or partly slatted floor, which produce liquid manure （a mixture of urine, faeces, and cleaning water） whereas in China open stables with closed concrete floors are used with separate collection of faeces and urine. Based on data from literature and own analyses nutrient flows of both systems are calculated. The Chinese manure system or mechanized belt or V-shaped pit floor systems with faeces/urine separation are more effective to separate solids, nutrients, carbon, and heavy metal than mechanical separation from liquid manure. The separation rates can be up to 88% for the solids, 67% for nitrogen, 93% for phosphorus, 60% for potassium, 94% for magnesium, 97% for cupper, and 98% for zinc. The faeces can be used for composting to produce a market product.
基金supported by the National Natural Science Foundation ofChina(40974020,40074024)National 973 Project of China2008(B425704)State Key Laboratory of Earthguake Dynamics Project(LED2008B02)
文摘The dynamic process of the 2008 Wenehuan earthquake is simulated by finite-element method （ FEM）, and the results suggest that we may be able to estimate the occurrence time, location, and magnitude of similar earthquakes in the future. Thus a numerical earthquake prediction （NEP） program is proposed; the reliability of it is dependent on its gradual refinement and the parameters used in the models.
