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当下中国宪法司法化的路径与方法 被引量:81
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作者 上官丕亮 《现代法学》 CSSCI 北大核心 2008年第2期3-16,共14页
法院适用法律必须解释法律,而解释法律又必须进行合宪解释,合宪解释是目前我国宪法在司法中适用的最好方式,是现行体制下我国宪法司法化的最佳路径。各级人民法院在民事、刑事和行政诉讼中审理每一起案件适用法律时都应当考虑到宪法,进... 法院适用法律必须解释法律,而解释法律又必须进行合宪解释,合宪解释是目前我国宪法在司法中适用的最好方式,是现行体制下我国宪法司法化的最佳路径。各级人民法院在民事、刑事和行政诉讼中审理每一起案件适用法律时都应当考虑到宪法,进行合宪解释。每一位法官都是合宪解释的主体,都享有法律解释权和宪法解释权,但这并不否定最高人民法院的最高司法解释权以及全国人大常委会对法律和宪法的最终解释权。法院通过合宪解释方式间接适用宪法,不必将宪法作为裁判依据而引用,但应当在裁判说理部分引用宪法条款。法院在合宪解释过程中发现法律明显违宪时,应当中止诉讼,逐级上报,由最高人民法院报送全国人大常委会处理。 展开更多
关键词 宪法 司法化 路径 方法 合宪解释
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原始生殖细胞的人类胚胎干细胞克隆 被引量:40
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作者 常万存 窦忠英 +4 位作者 马鸿飞 杨春荣 高志敏 雷安民 樊敬庄 《西北农业大学学报》 CSCD 北大核心 1998年第6期105-108,共4页
取材于妊娠终止胚胎生殖腺或生殖嵴及其周围组织,用DMEM+NCS培养液体外培养,分离由人PGCs转化成的类ES细胞,并将其与同源胚胎成纤维细胞一起用胰蛋白酶+EDTA的无钙镁PBS液消化传代。在原代培养12h观察到胞... 取材于妊娠终止胚胎生殖腺或生殖嵴及其周围组织,用DMEM+NCS培养液体外培养,分离由人PGCs转化成的类ES细胞,并将其与同源胚胎成纤维细胞一起用胰蛋白酶+EDTA的无钙镁PBS液消化传代。在原代培养12h观察到胞体较大、边缘不整、贴壁分裂增殖的PGCs样细胞,48h后观察到26处紧密排列呈鸟巢状的类ES细胞集落及集落团。第6d传代成功,第16d传至第4代。结果表明,体外培养附植后胚胎能够建成人的类ES细胞系。 展开更多
关键词 原始生殖细胞 胚胎干细胞 克隆 体外培养
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银行信贷退出理论和实践研究 被引量:31
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作者 姜建清 《金融研究》 CSSCI 北大核心 2004年第1期1-18,共18页
企业生命周期理论和银行的信贷经营原则要求银行业在信贷经营过程中必须实施信贷退出。但目前无论从理论上,还是在我国银行业的实践中,信贷退出尚没有系统的研究成果。缺乏退出的银行信贷业务往往伴随企业的成长一直走到它的生命周期尽... 企业生命周期理论和银行的信贷经营原则要求银行业在信贷经营过程中必须实施信贷退出。但目前无论从理论上,还是在我国银行业的实践中,信贷退出尚没有系统的研究成果。缺乏退出的银行信贷业务往往伴随企业的成长一直走到它的生命周期尽头,付出了沉重代价。本文拟对信贷退出的对象、决策、通道、壁垒等进行系统研究,并在此基础上讨论我国银行业的信贷退出实践,以填补信贷退出研究领域的空白,为我国银行业向现代商业银行的转变做出贡献。 展开更多
关键词 信贷退出 企业生命周期 异化 通道 壁垒
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癌毒转移的中医理论探讨 被引量:47
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作者 叶乃菁 刘宣 李琦 《中医杂志》 CSCD 北大核心 2014年第3期185-188,共4页
中医学认为,癌毒是恶性肿瘤的致病因素,癌毒在体内的转移造成了恶性肿瘤的转移。生理情况下,人体存在着经络、气街、四海通道,以及由内到外的三焦、膜原、腠理通道,为人体运输各种物质。病理情况下,正气亏虚,癌毒可以侵入这些通道进行... 中医学认为,癌毒是恶性肿瘤的致病因素,癌毒在体内的转移造成了恶性肿瘤的转移。生理情况下,人体存在着经络、气街、四海通道,以及由内到外的三焦、膜原、腠理通道,为人体运输各种物质。病理情况下,正气亏虚,癌毒可以侵入这些通道进行转移。现代医学认为,恶性肿瘤可通过血管、淋巴管等途径进行转移,不同的转移途径与肿瘤预后密切相关。因此,依据中医的通道概念研究癌毒的转移,对恶性肿瘤转移的防治具有重要意义。 展开更多
关键词 癌毒 肿瘤转移 经络 三焦
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基于RNG湍流模型的双向泵站出水流道流动计算 被引量:36
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作者 成立 刘超 +1 位作者 周济人 汤方平 《水科学进展》 EI CAS CSCD 北大核心 2004年第1期109-112,共4页
低扬程双向流道泵站是我国独创的泵站型式,为深入了解其流道内流场特性,首次运用基于壁面律的RNGk ε的湍流模型对流道进行数值模拟。计算采用交错网格的有限体积法求解三维不可压Navier Stokes方程。提出优化出水流道的目标函数,并依... 低扬程双向流道泵站是我国独创的泵站型式,为深入了解其流道内流场特性,首次运用基于壁面律的RNGk ε的湍流模型对流道进行数值模拟。计算采用交错网格的有限体积法求解三维不可压Navier Stokes方程。提出优化出水流道的目标函数,并依据数值模拟结果,预测出水流道水力损失。根据经验公式计算的水力损失和预测的出水流道水力损失两者较为接近,表明通过采用基于壁面律的RNG湍流模型能较好地预测出水流道的水力性能。考虑壁面律的RNG湍流模型在泵站流道内特性数值模拟和外特性预测研究方面有较好的推广价值。 展开更多
关键词 双向泵站 RNG湍流模型 出水流道 壁面律 数值模拟
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高速公路通道软基低强度混凝土桩处理试验研究 被引量:22
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作者 曾开华 俞建霖 龚晓南 《岩土工程学报》 EI CAS CSCD 北大核心 2003年第6期715-719,共5页
在国内首次采用低强度混凝土桩复合地基加固高速公路通道深厚软土地基,解决通道地基处理中的二次开挖、桥头跳车等问题。介绍了在杭宁高速公路通道地基低强度混凝土桩处理中进行的桩土应力、桩土表面沉降、分层沉降、侧向变形等项目的... 在国内首次采用低强度混凝土桩复合地基加固高速公路通道深厚软土地基,解决通道地基处理中的二次开挖、桥头跳车等问题。介绍了在杭宁高速公路通道地基低强度混凝土桩处理中进行的桩土应力、桩土表面沉降、分层沉降、侧向变形等项目的现场测试成果,并以此对路堤荷载作用下低强度混凝土桩复合地基的性状及其处理效果进行分析。 展开更多
关键词 高速公路 软土地基 混凝土桩 路堤 通道 复合地基 地基沉降
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Analytical method for softness abrasive flow field based on discrete phase model 被引量:29
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作者 JI ShiMing1,2, XIAO FengQing1,2 & TAN DaPeng1,2 1Key Laboratory of Special Purpose Equipment and Advanced Manufacturing Technology, (Zhejiang University of Technology), Ministry of Education, Hangzhou 310014, China 2Key Laboratory of Special Purpose Equipment and Advanced Manufacturing Technology of Zhejiang Province, Hangzhou 310014, China 《Science China(Technological Sciences)》 SCIE EI CAS 2010年第10期2867-2877,共11页
Aiming at the problem of difficult contact finishing for mini structural surface in course of mould manufacturing,a new no-tool precision machining method based on soft abrasive flow machining (SAFM) was proposed. It ... Aiming at the problem of difficult contact finishing for mini structural surface in course of mould manufacturing,a new no-tool precision machining method based on soft abrasive flow machining (SAFM) was proposed. It allocated restrained component near surface machined,constituted restrained abrasive flow passage,and made the surface become a segment of passage wall. It could control turbulence abrasive flow in restrained passage,realize micro cutting for passage wall,and utilize the irregular motion of abrasive flow to eliminate the mono-directional marks on machined surfaces,and the precision could reach the specular level. A two-phase dynamic model of abrasive flow oriented to SAFM combined with discrete phase model (DPM) was established,the law of two-phase flow motion and the related physical parameters was obtained by corresponding numerical simulation method,and the mechanism of precision machining in SAFM was discussed. Simulation results show that the abrasive flow machining process mainly appears as translation of ablating location with the influence by granular pressure,and as the variation of machining efficiency with the influence by near-wall particle velocity. Thus via control of the inlet velocity and its corresponding machining time,it is supposed to work out the machining process according to the machining requirements by using the Preston equation to seek the relationship among velocity,pressure and material removing rate. By tracking near-wall particles,it can be confirmed that the movement of near-wall abrasive particles is similar to stream-wise vortices. The cutting traces on workpiece surfaces assume disorderly arrangement,so the feasibility of the SAFM method can be reaffirmed. 展开更多
关键词 structural FLOW passage ULTRA-PRECISION MACHINING SOFTNESS ABRASIVE FLOW discrete phase model
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箱涵式进水流道的立式轴流泵装置水动力特性分析 被引量:25
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作者 杨帆 刘超 +2 位作者 汤方平 周济人 罗灿 《农业工程学报》 EI CAS CSCD 北大核心 2014年第4期62-69,共8页
为了研究有涡时箱涵式进水流道的立式轴流泵装置水动力性能,该文采用CFD(computational fluid dynamic)和高速摄影技术对箱涵式进水流道的立式轴流泵装置进水流道内部附底涡流动特征及其对泵装置水动力性能的影响进行了分析。数值模拟... 为了研究有涡时箱涵式进水流道的立式轴流泵装置水动力性能,该文采用CFD(computational fluid dynamic)和高速摄影技术对箱涵式进水流道的立式轴流泵装置进水流道内部附底涡流动特征及其对泵装置水动力性能的影响进行了分析。数值模拟和试验结果表明,基于CFD数值模拟技术,成功捕捉到各工况时箱涵式进水流道内部附底涡轨迹,与高速摄影捕捉到附底涡的运动轨迹较为一致,且均发生于喇叭管口下方,附底涡对泵装置的安全运行稳定性有直接影响。有、无消涡锥的箱涵式进水流道出口断面的轴向速度分布均匀度与速度加权平均角的差异性较小,但其水力损失值下降较大;叶轮所受轴向力相对比值m1在0~7.0%范围内波动,轴向力随流量系数的增大而减小,附底涡对叶轮所受径向力的影响较大,径向力相对比值m2在5.0%~110.0%范围内波动,涡带对叶轮受力有一定程度的影响,实际工程中应避免箱涵式进水流道内部涡带的出现。 展开更多
关键词 数值分析 流场 轴流泵 流道 涡带 试验
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Effects of Meridional Flow Passage Shape on Hydraulic Performance of Mixed-flow Pump Impellers 被引量:19
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作者 BING Hao CAO Shuliang +1 位作者 TAN Lei ZHU Baoshan 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2013年第3期469-475,共7页
During the process of designing the mixed-flow pump impeller, the meridional flow passage shape directly affects the obtained meridional flow field, which then has an influence on the three-dimensional impeller shape.... During the process of designing the mixed-flow pump impeller, the meridional flow passage shape directly affects the obtained meridional flow field, which then has an influence on the three-dimensional impeller shape. However, the meridional flow passage shape is too complicated to be described by a simple formula for now. Therefore, reasonable parameter selection for the meridional flow passage is essential to the investigation. In order to explore the effects of the meridional flow passage shape on the impeller design and the hydraulic performance of the mixed-flow pump, the hub and shroud radius ratio (HSRR) of impeller and the outlet diffusion angle (ODA) of outlet zone are selected as the meridional flow passage parameters. 25 mixed-flow pump impellers, with specific speed of 496 under the design condition, are designed with various parameter combinations. Among these impellers, one with HSRR of 1.94 and ODA of 90° is selected to carry out the model test and the obtained experimental results are used to verify accuracies of the head and the hydraulic efficiency predicted by numerical simulation. Based on SIMPLE algorithm and standard k-ε two-equation turbulence model, the three-dimensional steady incompressible Reynolds averaged Navier-Stokes equations are solved and the effects of different parameters on hydraulic performance of mixed-flow pump impellers are analyzed. The analysis results demonstrate that there are optimal values of HSRR and ODA available, so the hydraulic performance and the internal flow of mixed-flow pumps can be improved by selecting appropriate values for the meridional flow passage parameters. The research on these two parameters, HSRR and ODA, has further illustrated influences of the meridional flow passage shape on the hydraulic performance of the mixed-flow pump, and is beneficial to improving the design of the mixed-flow pump impeller. 展开更多
关键词 mixed-flow pump meridional flow passage numerical simulation hydraulic performance
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NONLINEAR STOCHASTIC DYNAMICS: A SURVEY OF RECENT DEVELOPMENTS 被引量:18
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作者 朱位秋 蔡国强 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2002年第6期551-566,共16页
This paper provides an overview of significant advances in nonlinear stochastic dynamics during the past two decades, including random response, stochastic stability, stochastic bifurcation, first passage problem and ... This paper provides an overview of significant advances in nonlinear stochastic dynamics during the past two decades, including random response, stochastic stability, stochastic bifurcation, first passage problem and nonlinear stochastic control. Topics for future research are also suggested. 展开更多
关键词 nonlinear system random vibration stochastic stability stochastic bifurcation first passage problem
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Pressure fluctuation propagation of a pump turbine at pump mode under low head condition 被引量:19
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作者 GUO Lei LIU JinTao +2 位作者 WANG LeQin QIN DaQing WEI XianZhu 《Science China(Technological Sciences)》 SCIE EI CAS 2014年第4期811-818,共8页
Pressure fluctuation at the vaneless space and vanes passages is one of the most important problems for the stable operation of a pump turbine. The fluctuation appears in any operating condition. Much research has bee... Pressure fluctuation at the vaneless space and vanes passages is one of the most important problems for the stable operation of a pump turbine. The fluctuation appears in any operating condition. Much research has been done on the pressure fluctuation of hydraulic machinery. However, the details of pressure fluctuation propagation of the pump turbine at the pump mode have not been revealed. The modem pump turbine with high water head requires the runner to be "flat", which would induce pressure fluctuation more easily than the low head pump turbine. In this article, a high head pump turbine model is used as the re- search object. As the pressure fluctuation at off-design point is more serious than at the design point, the low head condition is chosen as the research condition. Pressure fluctuation at the vaneless space and vanes passages is predicted by the computa- tional fluid dynamics method based on k-co shear stress transport model. The experiment conducted on the test rig of the Har- bin Institute of Large Electrical Machinery is used to verify the simulation method. It proves that the numerical method is a feasible way to research the fluctuation under this operating condition. The pressure fluctuation along the passage direction is analyzed at time and frequency domains. It is affected mainly by the interaction between the runner and vanes. In the circumferential direction, the influence of the special stay vane on the pressure fluctuation is got. The amplitude in the high-pressure side passage of that vane is lower than that in the other side. The study provides a basic understanding of the pressure fluctua- tion of a pump turbine and could be used as a reference to improve the operation stability of it. 展开更多
关键词 pump turbine pressure fluctuation vaneless space vanes passage pump mode low head
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双关范围新论 被引量:10
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作者 钟玖英 《南京晓庄学院学报》 2002年第1期94-99,共6页
双关是一个重要的传统辞格。对双关的范围 ,不同的时代有不同认识 ,一般认为双关存在于词语、句子层面 ,也有学者认为双关存在于语素、词、短语、句子等语言层面。其实双关广泛存在于语言的各级单位 :从语素到语段、语篇。语篇双关是文... 双关是一个重要的传统辞格。对双关的范围 ,不同的时代有不同认识 ,一般认为双关存在于词语、句子层面 ,也有学者认为双关存在于语素、词、短语、句子等语言层面。其实双关广泛存在于语言的各级单位 :从语素到语段、语篇。语篇双关是文学文本的重要特征 ,这与人类的交际方式、文本的生成规则和审美需求密切相关。 展开更多
关键词 双关 .范围 语段 语篇 文本
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Experimental study of flow field in interference area between impeller and guide vane of axial flow pump 被引量:16
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作者 张华 施卫东 +2 位作者 陈斌 张启华 曹卫东 《Journal of Hydrodynamics》 SCIE EI CSCD 2014年第6期894-901,共8页
Axial flow pump is a kind of typical pumps with rotor-stator interaction, thus the measurement of the flow field between impeller and guide vane would facilitate the study of the internal rotor-stator interaction mech... Axial flow pump is a kind of typical pumps with rotor-stator interaction, thus the measurement of the flow field between impeller and guide vane would facilitate the study of the internal rotor-stator interaction mechanism. Through a structural modification of a traditional axial flow pump, the requirements of particle image velocimetry(PIV) measurement are met. Under the condition of opt.0.8Q, the axial vortex is identified between impeller hub and guide vane hub, which is developed into the main flow and to affect the movement when the relative positions of impeller and guide vane at different flow rates are the same. Besides, the development and the dissipation of the tip leakage and the passage vortex in impeller passages are mainly responsible for the difference of the flow field close to the outer rim. As the flow rate decreases, the distribution of the meridional velocities at the impeller outlet becomes more non-uniform and the radial velocity component keeps increasing. The PIV measurement results under the condition of opt.1.0Q indicate that the flow separation and the trailing vortex at the trailing edge of a blade are likely to result in a velocity sudden change in this area, which would dramatically destroy the continuity of the flow field. Moreover, the radial direction of the flow between impeller and guide vane on the measurement plane does not always point from hub to rim. For a certain position, the direction is just from rim to hub, as is affected by the location of the intersection line of the shooting section and the impeller blade on the impeller as well as the angle between the intersection line and the rotating shaft. 展开更多
关键词 axial flow pump tip leakage passage vortex radial velocity axial velocity
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S形贯流泵装置多工况过流部件水力性能分析 被引量:17
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作者 杨帆 刘超 +2 位作者 汤方平 周济人 金燕 《农业机械学报》 EI CAS CSCD 北大核心 2014年第5期71-77,共7页
为深入研究S形轴伸贯流泵装置过流部件的水力性能,采用CFD技术对泵装置进行了多工况全流道的数值计算,分析了泵装置各过流部件的水力性能,重点阐述了3种特征工况(小流量工况KQ=0.368、最优工况KQ=0.490、大流量工况KQ=0.613)时转轮叶片... 为深入研究S形轴伸贯流泵装置过流部件的水力性能,采用CFD技术对泵装置进行了多工况全流道的数值计算,分析了泵装置各过流部件的水力性能,重点阐述了3种特征工况(小流量工况KQ=0.368、最优工况KQ=0.490、大流量工况KQ=0.613)时转轮叶片表面的静压分布、摩擦力线、各轴向弦长位置的轴向速度分布以及导叶体内部流态和回收环量效果。结果表明:在轮缘侧的叶片压力面静压值较大,在轮毂侧的叶片吸力面的静压值较大,轮缘侧较小,且随着展向位置Span值的增大,压力面与吸力面的压差呈现出逐渐递增趋势。在最优工况时,从导叶体进口至出口,导叶体的静压值逐渐增大。随流量的减小,导叶体的回收环量比CH先减小后增大。在最优工况KQ=0.490时,回收环量比CH最小,其值仅为0.031。针对该泵装置进行了同尺寸的物理模型试验,获得了泵装置的综合特性曲线,在叶片安放角-2°时,新型S形轴伸贯流泵装置的最高效率达83.55%,此时流量系数KQ=0.443,扬程系数KH=0.828。通过物理模型试验结果对泵装置外特性预测结果进行了验证,对比分析结果表明数值模拟是可信的。 展开更多
关键词 贯流泵 叶轮 导叶体 流道 水力性能 数值模拟
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神经干细胞培养及其影响因素 被引量:17
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作者 徐富翠 邹礼乐 +1 位作者 梅欣明 郭勇 《中国组织工程研究》 CAS CSCD 2013年第10期1835-1840,共6页
背景:神经干细胞移植治疗是中枢神经系统损伤性、难治性疾病研究的热点。如何有效提高神经干细胞存活率、克隆形成率将是其应用的重要基础和前提。目的:探讨影响神经干细胞培养质量的可能因素,为神经干细胞的基础和临床应用研究提供参... 背景:神经干细胞移植治疗是中枢神经系统损伤性、难治性疾病研究的热点。如何有效提高神经干细胞存活率、克隆形成率将是其应用的重要基础和前提。目的:探讨影响神经干细胞培养质量的可能因素,为神经干细胞的基础和临床应用研究提供参考。方法:①神经干细胞分离和培养:分离出生24h内SD大鼠的大脑,剪碎离心后以1×108L-1的浓度接种,经含体积分数为10%胎牛血清的DMEM/F12培养基预培养4h后改用无血清条件培养基(DMEM/F12,碱性成纤维细胞生长因子、表皮生长因子、B27)培养,2d或3d换液1次,6d时传代。②神经干细胞鉴定:倒置显微镜下观察细胞形态及生物学特性;免疫组化检测第3代细胞神经巢蛋白表达和诱导分化后神经元特异性烯醇化酶和神经胶质酸性蛋白的表达。结果与结论:出生24h的新生大鼠可获得足量的神经干细胞;血清预培养可提高神经干细胞的增殖和存活率;免疫组化结合血清诱导分化可对培养的神经干细胞进行定性鉴定。取材时间、细胞接种密度、传代时间、细胞因子、血清等多种因素都将影响神经干细胞的培养质量。 展开更多
关键词 干细胞 干细胞培养与分化 神经干细胞 培养 取材时间 细胞密度 血清 传代 细胞因子 影响因素 其他基金 干细胞图片文章
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An Adaptive Rapidly-Exploring Random Tree 被引量:15
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作者 Binghui Li Badong Chen 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2022年第2期283-294,共12页
Sampling-based planning algorithms play an important role in high degree-of-freedom motion planning(MP)problems,in which rapidly-exploring random tree(RRT)and the faster bidirectional RRT(named RRT-Connect)algorithms ... Sampling-based planning algorithms play an important role in high degree-of-freedom motion planning(MP)problems,in which rapidly-exploring random tree(RRT)and the faster bidirectional RRT(named RRT-Connect)algorithms have achieved good results in many planning tasks.However,sampling-based methods have the inherent defect of having difficultly in solving planning problems with narrow passages.Therefore,several algorithms have been proposed to overcome these drawbacks.As one of the improved algorithms,Rapidlyexploring random vines(RRV)can achieve better results,but it may perform worse in cluttered environments and has a certain environmental selectivity.In this paper,we present a new improved planning method based on RRT-Connect and RRV,named adaptive RRT-Connect(ARRT-Connect),which deals well with the narrow passage environments while retaining the ability of RRT algorithms to plan paths in other environments.The proposed planner is shown to be adaptable to a variety of environments and can accomplish path planning in a short time. 展开更多
关键词 Narrow passage path planning rapidly-exploring random tree(RRT)-Connect sampling-based algorithm
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Numerical simulation and analysis of solid-liquid two-phase threedimensional unsteady flow in centrifugal slurry pump 被引量:16
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作者 吴波 汪西力 +1 位作者 LIU Hui 徐海良 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第8期3008-3016,共9页
Based on RNG k-ε turbulence model and sliding grid technique, solid-liquid two-phase three-dimensional(3-D) unsteady turbulence of full passage in slurry pump was simulated by means of Fluent software. The effects of... Based on RNG k-ε turbulence model and sliding grid technique, solid-liquid two-phase three-dimensional(3-D) unsteady turbulence of full passage in slurry pump was simulated by means of Fluent software. The effects of unsteady flow characteristics on solid-liquid two-phase flow and pump performance were researched under design condition. The results show that clocking effect has a significant influence on the flow in pump, and the fluctuation of flow velocity and pressure is obvious, particularly near the volute tongue, at the position of small sections of volute and within diffuser. Clocking effect has a more influence on liquid-phase than on solid-phase, and the wake-jet structure of relative velocity of solid-phase is less obvious than liquid-phase near the volute tongue and the impeller passage outlet. The fluctuation of relative velocity of solid-phase flow is 7.6% smaller than liquid-phase flow at the impeller outlet on circular path. Head and radial forces of the impeller are 8.1% and 85.7% of fluctuation, respectively. The results provide a theoretical basis for further research for turbulence, improving efficient, reducing the hydraulic losses and wear. Finally, field tests were carried out to verify the operation and wear of slurry pump. 展开更多
关键词 slurry pump solid-liquid two-phase flow unsteady flow 3-D full passage numerical simulation
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狂犬病毒疫苗株CTN-1八代次N和G基因序列测定及分析 被引量:14
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作者 孟胜利 徐葛林 +4 位作者 明平刚 孙文 吴杰 杨晓明 严家新 《中国人兽共患病学报》 CAS CSCD 北大核心 2007年第4期327-332,335,共7页
目的测定八个代次CTN-1疫苗株核蛋白(N)和糖蛋白(G)基因序列,并与代表性疫苗株和街毒株进行比较。方法用RT-PCR反应扩增八代次CTN-1的N和G基因,获得cDNA,进行序列测定。结果八个代次CTN-1的N和G基因序列与其它疫苗株比较,核苷... 目的测定八个代次CTN-1疫苗株核蛋白(N)和糖蛋白(G)基因序列,并与代表性疫苗株和街毒株进行比较。方法用RT-PCR反应扩增八代次CTN-1的N和G基因,获得cDNA,进行序列测定。结果八个代次CTN-1的N和G基因序列与其它疫苗株比较,核苷酸的同源性分别为82.7%~84.5%和86.6%~88.5%,氨基酸同源性分别为88.9%~92.5%和95.3%~98.2%;与在我国分离的街毒株相比,核苷酸的同源性分别为84.2%~95.0%和80.4%~94.3%,氨基酸同源性分别为92.1%~99.6%和88.7%~98.5%。CTN-1株八个代次之间的核苷酸序列几乎完全相同。结论CTN-1株在传代过程中N和G基因变异小,与中国流行的代表性街毒株的同源性高于其它疫苗株。 展开更多
关键词 狂犬病毒 传代 CTN-1 序列测定
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竖井型线演变及对泵装置水力性能的影响分析 被引量:15
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作者 杨帆 刘超 +1 位作者 汤方平 周济人 《应用基础与工程科学学报》 EI CSCD 北大核心 2014年第1期129-138,共10页
为研究系列竖井型线的演变规律及其对贯流泵装置内流机理及运行稳定性的影响,在归纳分析竖井型线演变的基础上采用一维水力设计方法设计了4种型体的竖井流道.基于ANSYS CFX对4种不同竖井型体的贯流泵装置进行了三维湍流场的数值计算,定... 为研究系列竖井型线的演变规律及其对贯流泵装置内流机理及运行稳定性的影响,在归纳分析竖井型线演变的基础上采用一维水力设计方法设计了4种型体的竖井流道.基于ANSYS CFX对4种不同竖井型体的贯流泵装置进行了三维湍流场的数值计算,定量分析了4种竖井流道的水力性能.引入无因次动量参数分析法对比分析了各竖井流道对泵装置运行稳定性的影响,流量系数K Q在0.317—0.634范围内各方案的泵装置动量参数均随流量系数增大而减小,4种竖井流道对泵装置运行稳定性的影响差异性很小.提出了泵装置多工况性能加权评价指标(M.P.I)定量分析了不同竖井流道对多工况运行时泵装置综合水力性能的影响,该方法可用于不同泵装置水力性能的分析及泵装置方案的优选.为验证数值计算的准确性和可靠性,对泵装置进行了物理模型试验,对比分析结果表明,数值计算的结果是可信的. 展开更多
关键词 水力机械 贯流泵 流道 水力性能 数值分析 评价模型
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Influence of coupled boundary layer suction and bowed blade on flow field and performance of a diffusion cascade 被引量:12
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作者 Cao Zhiyuan Liu Bo +2 位作者 Zhang Ting Yang Xiqiong Chen Pingping 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2017年第1期249-263,共15页
Based on the investigation of mid-span local boundary layer suction and positive bowed cascade, a coupled local tailored boundary layer suction and positive bowed blade method is developed to improve the performance o... Based on the investigation of mid-span local boundary layer suction and positive bowed cascade, a coupled local tailored boundary layer suction and positive bowed blade method is developed to improve the performance of a highly loaded diffusion cascade with less suction slot. The effectiveness of the coupled method under different inlet boundary layers is also investigated.Results show that mid-span local boundary layer suction can effectively remove trailing edge separation, but deteriorate the flow fields near the endwall. The positive bowed cascade is beneficial for reducing open corner separation, but is detrimental to mid-span flow fields. The coupled method can further improve the performance and flow field of the cascade. The mid-span trailing edge separation and open corner separation are eliminated. Compared with linear cascade with suction, the coupled method reduces overall loss of the cascade by 31.4% at most. The mid-span loss of the cascade decreases as the suction coefficient increases, but increases as bow angle increases. The endwall loss increases as the suction coefficient increases. By contrast, the endwall loss decreases significantly as the bow angle increases. The endwall loss of coupled controlled cascade is higher than that of bowed cascade with the same bow angle because of the spanwise inverse ‘‘C" shaped static pressure distribution. Under different inlet boundary layer conditions, the coupled method can also improve the cascade effectively. 展开更多
关键词 Axial compressor Boundary layer suction Bowed blade Corner separation Coupled method passage vortex
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