Soybean mosaic virus （SMV） is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean （Glycine max （L.） Merr.）. SMV Strain SC14 isolated from Sh...Soybean mosaic virus （SMV） is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean （Glycine max （L.） Merr.）. SMV Strain SC14 isolated from Shanxi Province, China, was a newly identified virulent strain and can infect Kefeng No. 1, a source with wide spectrum resistance. In the present study, soybean accessions, PI96983, Qihuang No. 1 and Qihuang No. 22 were identified to be resistant （R） and Nannong 1138-2, Pixianchadou susceptible （S） to SC14. Segregation analysis of PI96983 x Nannong 1138-2 indicated that a single dominant gene （designated as Rsc14） controlled the resistance to SC14 at both V2 and R1 developmental stages. The same results were obtained for the crosses of Qihuang No. 1 × Nannong 1138-2 and Qihuang No. 22 x Nannong 1138-2 as in PI96983 x×Nannong 1138-2 at V2 stage, but at R1 stage, the F1 performed as necrosis （a susceptible symptom other than mosaic）, F2 segregated in a ratio of 1R：2N：IS, and the progenies of necrotic （N） F2 individuals segregated also in R, N and S. It indicated that a single gene （designated as Rsc140, to be different from that of PI96983） controlled the resistance to SC14, its dominance was the same as in PI96983 × Nannong 1138-2 （without symptoms） at V2 stage and not the same at R1 stage. The tightly linked co-dominant simple sequence repeat （SSR） marker Satt334 indicated that all the heterozygous bands were completely corresponding to the necrotic F2 individuals, or all the necrotic F2 individuals were heterozygotes. It was inferred that necrosis might be due to the interaction among SMV strains, resistance genes, genetic background of the resistance genes, and plant development stage. Furthermore, the bulked segregant analysis （BSA） of SSR markers was conducted to map the resistance genes. In F2 of PI96983 × Nannong 1138-2, five SSR markers, Sat_297, Sat_234, Sat_154, Sct_033 and Sat_120, were found closely linked to Rsc14, with genetic distances of展开更多
Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral dise...Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral diseases of sugarcane. In the study, four primer pairs specific to SCMV, SrMV, MDMV and JGMV, respectively, were designed and used to detect 29 sugarcane leaf mosaic samples collected from 9 locations in Jiangxi province. The representative RT-PCR products were sequenced. The results showed that 22 samples were infected by SCMV, three by SrMV, and four were mix-infected by SCMV and SrMV. MDMV or JGMV were not identified in all samples. The result indicates that SCMV is the major pathogen of sugarcane mosaic disease in Jiangxi province, and SrMV is also a pathogen for the disease.展开更多
Tobacco ( Nicotiana tabacum L.) “NC89” plants were transformed with deletion mutant of cucumber mosaic virus (CMV) movement protein (MP) gene and full_length CMV MP gene, respectively. The transformed plants...Tobacco ( Nicotiana tabacum L.) “NC89” plants were transformed with deletion mutant of cucumber mosaic virus (CMV) movement protein (MP) gene and full_length CMV MP gene, respectively. The transformed plants were analyzed with polymerase chain reaction (PCR), PCR_Southern, Southern and Western blots. R 0 generation of the transgenic plants were inoculated with CMV. Five out of 10 lines of tobacco plants (BMPK) transformed with CMV MP deletion mutant gene showed high resistance to CMV infection and remained symptomless for up to 50 days post_inoculation. In contrast, tobacco plants (BMPR) transformed with full_length CMV MP gene did not show resistance to CMV infection. However, most of the infected full_length CMV MP gene transgenic plants recovered by showing none or very mild mosaic symptoms in 40 days post_inoculation. The results of R 1 generation of the BMPK transgenic plants tested under field conditions showed that all 5 lines of transgenic plants could delay the virus disease development.展开更多
基金Supported by the State Key Basic Research and Development Plan of China （2004CB117203-2 and 2002CB111304）, the National Natural Science Foundation of China （30571176 and 30490250）, the Natural Science Foundation of Jiangsu Province （BK2004100） and the Program for Changjiang Scholars and Innovative Research Team in University （PCSIRT）.
文摘Soybean mosaic virus （SMV） is one of the most broadly distributed diseases worldwide. It causes severe yield loss and seed quality deficiency in soybean （Glycine max （L.） Merr.）. SMV Strain SC14 isolated from Shanxi Province, China, was a newly identified virulent strain and can infect Kefeng No. 1, a source with wide spectrum resistance. In the present study, soybean accessions, PI96983, Qihuang No. 1 and Qihuang No. 22 were identified to be resistant （R） and Nannong 1138-2, Pixianchadou susceptible （S） to SC14. Segregation analysis of PI96983 x Nannong 1138-2 indicated that a single dominant gene （designated as Rsc14） controlled the resistance to SC14 at both V2 and R1 developmental stages. The same results were obtained for the crosses of Qihuang No. 1 × Nannong 1138-2 and Qihuang No. 22 x Nannong 1138-2 as in PI96983 x×Nannong 1138-2 at V2 stage, but at R1 stage, the F1 performed as necrosis （a susceptible symptom other than mosaic）, F2 segregated in a ratio of 1R：2N：IS, and the progenies of necrotic （N） F2 individuals segregated also in R, N and S. It indicated that a single gene （designated as Rsc140, to be different from that of PI96983） controlled the resistance to SC14, its dominance was the same as in PI96983 × Nannong 1138-2 （without symptoms） at V2 stage and not the same at R1 stage. The tightly linked co-dominant simple sequence repeat （SSR） marker Satt334 indicated that all the heterozygous bands were completely corresponding to the necrotic F2 individuals, or all the necrotic F2 individuals were heterozygotes. It was inferred that necrosis might be due to the interaction among SMV strains, resistance genes, genetic background of the resistance genes, and plant development stage. Furthermore, the bulked segregant analysis （BSA） of SSR markers was conducted to map the resistance genes. In F2 of PI96983 × Nannong 1138-2, five SSR markers, Sat_297, Sat_234, Sat_154, Sct_033 and Sat_120, were found closely linked to Rsc14, with genetic distances of
文摘Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral diseases of sugarcane. In the study, four primer pairs specific to SCMV, SrMV, MDMV and JGMV, respectively, were designed and used to detect 29 sugarcane leaf mosaic samples collected from 9 locations in Jiangxi province. The representative RT-PCR products were sequenced. The results showed that 22 samples were infected by SCMV, three by SrMV, and four were mix-infected by SCMV and SrMV. MDMV or JGMV were not identified in all samples. The result indicates that SCMV is the major pathogen of sugarcane mosaic disease in Jiangxi province, and SrMV is also a pathogen for the disease.
文摘Tobacco ( Nicotiana tabacum L.) “NC89” plants were transformed with deletion mutant of cucumber mosaic virus (CMV) movement protein (MP) gene and full_length CMV MP gene, respectively. The transformed plants were analyzed with polymerase chain reaction (PCR), PCR_Southern, Southern and Western blots. R 0 generation of the transgenic plants were inoculated with CMV. Five out of 10 lines of tobacco plants (BMPK) transformed with CMV MP deletion mutant gene showed high resistance to CMV infection and remained symptomless for up to 50 days post_inoculation. In contrast, tobacco plants (BMPR) transformed with full_length CMV MP gene did not show resistance to CMV infection. However, most of the infected full_length CMV MP gene transgenic plants recovered by showing none or very mild mosaic symptoms in 40 days post_inoculation. The results of R 1 generation of the BMPK transgenic plants tested under field conditions showed that all 5 lines of transgenic plants could delay the virus disease development.
