通过两种方案试制Φ350 mm Ti-1023合金棒材,分析了两种方案生产的棒材横、纵向组织、力学性能和探伤结果的差异。结果表明:铸锭开坯及中间锻造采用一次"高低高",成品锻造相变温度以下35～50℃进行,变形量大于60%的锻造工艺,...通过两种方案试制Φ350 mm Ti-1023合金棒材,分析了两种方案生产的棒材横、纵向组织、力学性能和探伤结果的差异。结果表明:铸锭开坯及中间锻造采用一次"高低高",成品锻造相变温度以下35～50℃进行,变形量大于60%的锻造工艺,可生产出满足X/BS5116标准性能要求的棒材,且棒材的组织均匀性好。展开更多
β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis,antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjt...β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis,antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags（EST） from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 c DNAs possessed open reading frames that encoded166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4,mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps.The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps.展开更多
目的探讨祛风明目丸对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)大鼠房水及视网膜组织中NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)、接头蛋白凋亡...目的探讨祛风明目丸对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)大鼠房水及视网膜组织中NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)、接头蛋白凋亡相关斑点样蛋白(apoptosis related spot like protein,ASC)、白细胞介素-1β(interleukin-1β,IL-1β)表达的影响。方法将70只SPF级雄性Lewis大鼠按随机数字表法取19只为空白组,其余为造模组,进行主动免疫,建立EAU大鼠模型,连续造模7 d后分别从造模组及空白组随机抽取3只大鼠取眼球行HE染色,进行组织病理学检查;将造模成功的EAU大鼠随机分为模型组、祛风明目丸低剂量组(简称低剂量组)和祛风明目丸正常剂量组(简称正常剂量组),每组16只。空白组、模型组予以蒸馏水灌胃,低剂量组和正常剂量组予以不同剂量的祛风明目丸药液灌胃,均干预14 d。自造模开始,每天于裂隙灯下观察眼前节变化,并采用Caspi临床分级标准评分;自灌胃起14 d后,ELISA检测房水中NLRP3、IL-1β水平,Western blot检测视网膜组织中ASC、NLRP3蛋白水平。结果Caspi临床分级评分结果示,自造模第3天起造模组评分均明显高于空白组(P<0.01),说明EAU模型制备成功。ELISA结果显示,与空白组相比,模型组房水中NLRP3、IL-1β水平升高(P<0.05);与模型组相比,正常剂量组及低剂量组两者含量均降低(P<0.05);正常剂量组与低剂量组差异无统计学意义(P>0.05)。Western blot结果显示,与空白组相比,模型组视网膜组织中ASC、NLRP3蛋白表达水平升高(P<0.05);与模型组相比,正常剂量组及低剂量组视网膜组织中ASC、NLRP3蛋白表达含量均下降(P<0.05);与正常剂量组相比,低剂量组视网膜组织ASC、NLRP3蛋白表达水平升高(P<0.05)。结论祛风明目丸可能通过降低NLRP3炎症小体及下游炎症因子的表达对EAU大鼠发挥一定的治疗作用。展开更多
文摘通过两种方案试制Φ350 mm Ti-1023合金棒材,分析了两种方案生产的棒材横、纵向组织、力学性能和探伤结果的差异。结果表明:铸锭开坯及中间锻造采用一次"高低高",成品锻造相变温度以下35～50℃进行,变形量大于60%的锻造工艺,可生产出满足X/BS5116标准性能要求的棒材,且棒材的组织均匀性好。
基金The National High Technology Research and Development Program(863 Program)of China under contract No.2012AA092205the Major State Basic Research Development Program(973 Program)of China under contract No.2012CB114403the China Agriculture Research System-47,and the Scientific Research Foundation of Third Institute of Oceanography,SOA under contract No.2011018
文摘β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis,antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags（EST） from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 c DNAs possessed open reading frames that encoded166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4,mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps.The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps.
文摘目的探讨祛风明目丸对实验性自身免疫性葡萄膜炎(experimental autoimmune uveitis,EAU)大鼠房水及视网膜组织中NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)、接头蛋白凋亡相关斑点样蛋白(apoptosis related spot like protein,ASC)、白细胞介素-1β(interleukin-1β,IL-1β)表达的影响。方法将70只SPF级雄性Lewis大鼠按随机数字表法取19只为空白组,其余为造模组,进行主动免疫,建立EAU大鼠模型,连续造模7 d后分别从造模组及空白组随机抽取3只大鼠取眼球行HE染色,进行组织病理学检查;将造模成功的EAU大鼠随机分为模型组、祛风明目丸低剂量组(简称低剂量组)和祛风明目丸正常剂量组(简称正常剂量组),每组16只。空白组、模型组予以蒸馏水灌胃,低剂量组和正常剂量组予以不同剂量的祛风明目丸药液灌胃,均干预14 d。自造模开始,每天于裂隙灯下观察眼前节变化,并采用Caspi临床分级标准评分;自灌胃起14 d后,ELISA检测房水中NLRP3、IL-1β水平,Western blot检测视网膜组织中ASC、NLRP3蛋白水平。结果Caspi临床分级评分结果示,自造模第3天起造模组评分均明显高于空白组(P<0.01),说明EAU模型制备成功。ELISA结果显示,与空白组相比,模型组房水中NLRP3、IL-1β水平升高(P<0.05);与模型组相比,正常剂量组及低剂量组两者含量均降低(P<0.05);正常剂量组与低剂量组差异无统计学意义(P>0.05)。Western blot结果显示,与空白组相比,模型组视网膜组织中ASC、NLRP3蛋白表达水平升高(P<0.05);与模型组相比,正常剂量组及低剂量组视网膜组织中ASC、NLRP3蛋白表达含量均下降(P<0.05);与正常剂量组相比,低剂量组视网膜组织ASC、NLRP3蛋白表达水平升高(P<0.05)。结论祛风明目丸可能通过降低NLRP3炎症小体及下游炎症因子的表达对EAU大鼠发挥一定的治疗作用。
