The key of tissue section technique is the selection and application of the embedding agent. Based on the specific characteristics of the tissue and outer skeleton of insects, a new kind of embedding agent, colophony ...The key of tissue section technique is the selection and application of the embedding agent. Based on the specific characteristics of the tissue and outer skeleton of insects, a new kind of embedding agent, colophony and paraffin (CP), was used without destroying the integrity of insect organs. The tissue can be sliced continuously by the use of CP embedding technique. It solves the 3 difficult problems in the serial section of the organ and tissue of insect, namely stiff materials, thin section and continuity of sections. These difficult problems are not solved by any unitary tissue section technique that is used now, including the paraffin section technique. By using the CP embedding technique, any parts or organs (like antenna, leg and wing) and even the whole body of insects (mini body) can be sliced up continuously. It will open up wide possibilities for the study of inner structure, the interrelations among organs, the micro-characteristics and the physiological functions of insects展开更多
目的探讨聚桂醇原液在组织中的病理变化过程和硬化剂的病理机制。方法取标准健康成年日本大耳白兔,在兔胃壁组织中分别注入1%(F组)、3%(H组)聚桂醇原液0.5 m L,根据试验设定的时间,在标注的胃壁组织上取出注射过的全层胃壁组织,经固定...目的探讨聚桂醇原液在组织中的病理变化过程和硬化剂的病理机制。方法取标准健康成年日本大耳白兔,在兔胃壁组织中分别注入1%(F组)、3%(H组)聚桂醇原液0.5 m L,根据试验设定的时间,在标注的胃壁组织上取出注射过的全层胃壁组织,经固定、切片、染色后,显微镜下观察其成纤维细胞及胶原纤维的变化,找到组织纤维硬化的基本病理变化过程。结果 F组在第5天开始可见浆膜有炎症反应并有着色较深的成纤维细胞增生;成纤维细胞数量第6天达高峰,以后逐渐减少,同时纤维细胞逐渐增多,胶原纤维也逐渐增多;第12天大量形成胶原纤维。H组在第3天黏膜下有少量成纤维细胞增生,到第4天达最高峰,以后逐渐减少,同时胶原纤维逐渐增多;第8天以后,成纤维细胞逐渐减少,变成纤维细胞,胶原纤维平稳增加。结论聚桂醇液在兔胃壁组织中可以产生明显的炎性反应过程,并最终产生大量的胶原物质,形成组织纤维化;这个过程与药物浓度无关;药物浓度与在组织中产生成纤维细胞的时间有关。展开更多
文摘The key of tissue section technique is the selection and application of the embedding agent. Based on the specific characteristics of the tissue and outer skeleton of insects, a new kind of embedding agent, colophony and paraffin (CP), was used without destroying the integrity of insect organs. The tissue can be sliced continuously by the use of CP embedding technique. It solves the 3 difficult problems in the serial section of the organ and tissue of insect, namely stiff materials, thin section and continuity of sections. These difficult problems are not solved by any unitary tissue section technique that is used now, including the paraffin section technique. By using the CP embedding technique, any parts or organs (like antenna, leg and wing) and even the whole body of insects (mini body) can be sliced up continuously. It will open up wide possibilities for the study of inner structure, the interrelations among organs, the micro-characteristics and the physiological functions of insects
文摘目的探讨聚桂醇原液在组织中的病理变化过程和硬化剂的病理机制。方法取标准健康成年日本大耳白兔,在兔胃壁组织中分别注入1%(F组)、3%(H组)聚桂醇原液0.5 m L,根据试验设定的时间,在标注的胃壁组织上取出注射过的全层胃壁组织,经固定、切片、染色后,显微镜下观察其成纤维细胞及胶原纤维的变化,找到组织纤维硬化的基本病理变化过程。结果 F组在第5天开始可见浆膜有炎症反应并有着色较深的成纤维细胞增生;成纤维细胞数量第6天达高峰,以后逐渐减少,同时纤维细胞逐渐增多,胶原纤维也逐渐增多;第12天大量形成胶原纤维。H组在第3天黏膜下有少量成纤维细胞增生,到第4天达最高峰,以后逐渐减少,同时胶原纤维逐渐增多;第8天以后,成纤维细胞逐渐减少,变成纤维细胞,胶原纤维平稳增加。结论聚桂醇液在兔胃壁组织中可以产生明显的炎性反应过程,并最终产生大量的胶原物质,形成组织纤维化;这个过程与药物浓度无关;药物浓度与在组织中产生成纤维细胞的时间有关。
