Background Retinol binding protein 4 （RBP4）, as an adipocyte secreted cytokine, was recently found to be inversely correlated with expression of glucose transporter 4 （GLUT4） in insulin resistance （IR） state and...Background Retinol binding protein 4 （RBP4）, as an adipocyte secreted cytokine, was recently found to be inversely correlated with expression of glucose transporter 4 （GLUT4） in insulin resistance （IR） state and to have an intimate relationship with IR and type 2 diabetes mellitus （T2DM）. The present study aimed to evaluate the anti-diabetic efficacy of cinnamaldehyde （Cin）, berberine （Ber）, and metformin （Met） as well as their impacts on the RBP4-GLUT4 system. Methods Rat models of T2DM were established by combination of intraperitoneal injection of low-dose streptozotocin and high fat diet induction. Rats were divided into five groups： the control group, the diabetes group, the diabetes＋Ber group, the diabetes＋Cin group, and the diabetes＋Met group. Western blotting was used to detect the serum or tissue RBP4 and GLUT4 protein levels. Results After treatment for four weeks, both Cin and Ber displayed significant hypolipidemic, hypoglycemic, and insulin sensitizing functions （P 〈0.01） compared with the control group. Their effects on lowering fasting plasma glucose （FPG）, low density lipoprotein-cholesterol （LDL-C） and homeostasis model assessment of insulin resistance （HOMA-IR） seem even better than that of Met. Cin and Ber markedly lowered serum RBP4 levels and up-regulated the expression of tissue GLUT4 protein, and Cin seemed more notable in affecting these two proteins. Conclusions Both Cin and Ber display an exciting anti-diabetic efficacy in this study and may be of great value for the treatment of type 2 diabetes. Their mechanisms involve the RBP4-GLUT4 system, during which the serum RBP4 levels are lowered and the expression of tissue GLUT4 protein is up-regulated.展开更多
目的探讨2型糖尿病(type 2 diabetes,T2DM)伴急性脑梗死(acute cerebral infarction, ACI)患者的凝血功能、血清视黄醇结合蛋白4(retinol binding protein 4, RBP4)、溶血磷脂酸(lysophosphatidic acid, LPA)、超敏C反应蛋白(hypersensi...目的探讨2型糖尿病(type 2 diabetes,T2DM)伴急性脑梗死(acute cerebral infarction, ACI)患者的凝血功能、血清视黄醇结合蛋白4(retinol binding protein 4, RBP4)、溶血磷脂酸(lysophosphatidic acid, LPA)、超敏C反应蛋白(hypersensitive C-reactive protein, hs-CRP)的变化及其意义。方法采用病例对照研究方法,选取2016年6月-2017年6月)珠海市中西医结合医院收治的90例T2DM伴ACI患者(A组)、单纯T2DM患者90例(B组)、健康体检对象90例(C组),采用对三组研究对象的血栓弹力图检测三组的凝血功能、检测三组的指标、血清RBP4、LPA、hs-CRP水平进行回顾性分析,并分析上述指标与A组患者神经功能缺损评分(NIHSS)的关系。结果B组和C组的R值、K值均显著的高于A组(P<0.05);B组和C组的Angle角、MA值均显著的低于A 组(P <0.05 );B 组和 C 组的反应时间(reaction time, R) R 值、凝固时间(solidification time, K) K 值、角度(Angle) Angle 角、图中两侧曲线的最宽距离(the maximum distance between two sides of a graph, MA )MA 值差异无统计学意义(P>0.05);B组和C组的血清RBP4、LPA、hs-CRP水平均显著的低于A组(P<0.05),C组的血清RBP4、LPA、hs-CRP水平均显著的低于B组(P<0.05);A组的R值、K值与患者NIHSS评分呈显著的负相关关系(P <0.05), A组的Angle角、MA值与患者NIHSS评分呈显著的正相关关系(P<0.05);A组的血清RBP4、hs-CRP水平与患者NIHSS评分呈显著的正相关关系(P <0.05), A组的LPA与患者NIHSS评分无相关关系(P<0.05)。结论T2DM伴ACI患者凝血功能、血清RBP4、LPA、hs-CRP水平较单纯T2DM患者及健康人群发生显著改变,并且与患者神经功能缺损程度关系具有一定的相关性。展开更多
文摘目的检测正常糖调节正常体重者(NW-NGR)和正常糖调节超重/肥胖者(OW/OB-NGR)、2型糖尿病(T2DM)及其亚组2型糖尿病正常体重组(NW-T2DM)和2型糖尿病伴超重/肥胖组(OW/OB- T2DM)的血清视黄醇结合蛋白4(RBP4)水平,并探讨RBP4与体脂,糖、脂代谢,胰岛素敏感性等的相关性。方法采用HOMA-IR评价各组胰岛素敏感性,测定受试者的体重指数(BMI),腰臀比(WHR),脂肪含量(Fat%),检测空腹状态下血清RBP4,血糖,HbA_(1C)血脂和胰岛素水平。结果校正年龄、性别后,OW/OB-NGR,T2DM及OW/OB-T2DM组显著高于NW-NGR组的血清RBP4[(29.85±6.60、29.70±5.89、30.86±6.11)mg/L vs(25.47±6.84)mg/L,均P＜0.05]。NW-T2DM与NW-NGR组间的血清RBP4差异无统计学意义(28.35±5.42 vs 25.47±6.84)mg/L,但显著低于OW/OB-T2DM(P＜0.05)。多元逐步回归分析发现,WHR(r^2=0.166,P＜0.01)、甘油三酯(r^2=0.188,p＜0.01)、年龄(r^2=0.205,P＜0.01)是血清RBP4的独立相关因素。结论肥胖者的血清RBP4水平显著升高；血清RBP4与WHR、甘油三酯、年龄呈正相关。
文摘Background Retinol binding protein 4 （RBP4）, as an adipocyte secreted cytokine, was recently found to be inversely correlated with expression of glucose transporter 4 （GLUT4） in insulin resistance （IR） state and to have an intimate relationship with IR and type 2 diabetes mellitus （T2DM）. The present study aimed to evaluate the anti-diabetic efficacy of cinnamaldehyde （Cin）, berberine （Ber）, and metformin （Met） as well as their impacts on the RBP4-GLUT4 system. Methods Rat models of T2DM were established by combination of intraperitoneal injection of low-dose streptozotocin and high fat diet induction. Rats were divided into five groups： the control group, the diabetes group, the diabetes＋Ber group, the diabetes＋Cin group, and the diabetes＋Met group. Western blotting was used to detect the serum or tissue RBP4 and GLUT4 protein levels. Results After treatment for four weeks, both Cin and Ber displayed significant hypolipidemic, hypoglycemic, and insulin sensitizing functions （P 〈0.01） compared with the control group. Their effects on lowering fasting plasma glucose （FPG）, low density lipoprotein-cholesterol （LDL-C） and homeostasis model assessment of insulin resistance （HOMA-IR） seem even better than that of Met. Cin and Ber markedly lowered serum RBP4 levels and up-regulated the expression of tissue GLUT4 protein, and Cin seemed more notable in affecting these two proteins. Conclusions Both Cin and Ber display an exciting anti-diabetic efficacy in this study and may be of great value for the treatment of type 2 diabetes. Their mechanisms involve the RBP4-GLUT4 system, during which the serum RBP4 levels are lowered and the expression of tissue GLUT4 protein is up-regulated.
文摘目的探讨2型糖尿病(type 2 diabetes,T2DM)伴急性脑梗死(acute cerebral infarction, ACI)患者的凝血功能、血清视黄醇结合蛋白4(retinol binding protein 4, RBP4)、溶血磷脂酸(lysophosphatidic acid, LPA)、超敏C反应蛋白(hypersensitive C-reactive protein, hs-CRP)的变化及其意义。方法采用病例对照研究方法,选取2016年6月-2017年6月)珠海市中西医结合医院收治的90例T2DM伴ACI患者(A组)、单纯T2DM患者90例(B组)、健康体检对象90例(C组),采用对三组研究对象的血栓弹力图检测三组的凝血功能、检测三组的指标、血清RBP4、LPA、hs-CRP水平进行回顾性分析,并分析上述指标与A组患者神经功能缺损评分(NIHSS)的关系。结果B组和C组的R值、K值均显著的高于A组(P<0.05);B组和C组的Angle角、MA值均显著的低于A 组(P <0.05 );B 组和 C 组的反应时间(reaction time, R) R 值、凝固时间(solidification time, K) K 值、角度(Angle) Angle 角、图中两侧曲线的最宽距离(the maximum distance between two sides of a graph, MA )MA 值差异无统计学意义(P>0.05);B组和C组的血清RBP4、LPA、hs-CRP水平均显著的低于A组(P<0.05),C组的血清RBP4、LPA、hs-CRP水平均显著的低于B组(P<0.05);A组的R值、K值与患者NIHSS评分呈显著的负相关关系(P <0.05), A组的Angle角、MA值与患者NIHSS评分呈显著的正相关关系(P<0.05);A组的血清RBP4、hs-CRP水平与患者NIHSS评分呈显著的正相关关系(P <0.05), A组的LPA与患者NIHSS评分无相关关系(P<0.05)。结论T2DM伴ACI患者凝血功能、血清RBP4、LPA、hs-CRP水平较单纯T2DM患者及健康人群发生显著改变,并且与患者神经功能缺损程度关系具有一定的相关性。
