RNA-Seq, a high-throughput （HT） sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In ...RNA-Seq, a high-throughput （HT） sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In this study, RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary. After lllurrlina-Solexa deep sequencing, 26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing. Gene structure refinement analysis showed that 7340 genes that were annotated in the yak genome could be extended at the 5＇ or 3＇ ends based on the alignments been the transcripts and the genome sequence. Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp, and 2267 of them were predicted to code proteins. BLAST analysis of the new transcripts showed that 1200-4933 mapped to the non-redundant （nr）, nucleotide （nt） and/or SwissProt sequence databases. Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus （41.4%）, Bos grunniens mutus （33.0%）, Ovis aries （6.3%）, Homo sapiens （2.8%）, Mus musculus （1.6%） and other species. Functional analy- sis showed that these expressed genes were involved in various Gene Ontology （GO） categories and Kyoto Encyclopedia of Genes and Genomes pathways. GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction. The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance. Moreover, the results confirmed that RNA-Seq HT technology is highly ad- vantageous in improving gene structure information and mining of new genes, as well as in providing valuable data to expand the yak genome information.展开更多
Different gestational age fetal follicles of Northeast China Fine-fleece Sheep were histologically studied. The results showed that boundaries between medulla and cortex was ambiguous at the eighth week and became obv...Different gestational age fetal follicles of Northeast China Fine-fleece Sheep were histologically studied. The results showed that boundaries between medulla and cortex was ambiguous at the eighth week and became obvious at the twelfth week. Primordial follicles could be found in 8-week fetal ovaries and some follicle cells became irregular cuboid in 12-week, but primary follicles could be found until 16-week. There was significant difference between 8-week and 12-week follicles in size (P < 0.01), most mitochondria were round, some diamond, irregular and juvenile ones could be found with the development of oocytes. Some of oocytes degenerated in each stage of development. The diameter of primary oocytes, primordial follicles and primordial follicular oocytes were larger in 12-16 weeks fetal ovaries than other times.展开更多
基金supported by the National Science&Technology Pillar Program of China(2012BAD13B06)the Special Fund for Agroscientific Research in the Public Interest(201203009)
文摘RNA-Seq, a high-throughput （HT） sequencing technique, has been used effectively in large-scale transcriptomic studies, and is particularly useful for improving gene structure information and mining of new genes. In this study, RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary. After lllurrlina-Solexa deep sequencing, 26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library. Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing. Gene structure refinement analysis showed that 7340 genes that were annotated in the yak genome could be extended at the 5＇ or 3＇ ends based on the alignments been the transcripts and the genome sequence. Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp, and 2267 of them were predicted to code proteins. BLAST analysis of the new transcripts showed that 1200-4933 mapped to the non-redundant （nr）, nucleotide （nt） and/or SwissProt sequence databases. Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus （41.4%）, Bos grunniens mutus （33.0%）, Ovis aries （6.3%）, Homo sapiens （2.8%）, Mus musculus （1.6%） and other species. Functional analy- sis showed that these expressed genes were involved in various Gene Ontology （GO） categories and Kyoto Encyclopedia of Genes and Genomes pathways. GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction. The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance. Moreover, the results confirmed that RNA-Seq HT technology is highly ad- vantageous in improving gene structure information and mining of new genes, as well as in providing valuable data to expand the yak genome information.
文摘Different gestational age fetal follicles of Northeast China Fine-fleece Sheep were histologically studied. The results showed that boundaries between medulla and cortex was ambiguous at the eighth week and became obvious at the twelfth week. Primordial follicles could be found in 8-week fetal ovaries and some follicle cells became irregular cuboid in 12-week, but primary follicles could be found until 16-week. There was significant difference between 8-week and 12-week follicles in size (P < 0.01), most mitochondria were round, some diamond, irregular and juvenile ones could be found with the development of oocytes. Some of oocytes degenerated in each stage of development. The diameter of primary oocytes, primordial follicles and primordial follicular oocytes were larger in 12-16 weeks fetal ovaries than other times.
