目的探讨应对方式及家庭功能对新生儿重症监护室(neonatal intensive care unit,NICU)患儿家属心理健康状况影响的交互作用。方法便利抽样选取2016年10月至2017年10月哈尔滨市某三级甲等医院NICU就诊的患儿家属193名,运用症状自评量表...目的探讨应对方式及家庭功能对新生儿重症监护室(neonatal intensive care unit,NICU)患儿家属心理健康状况影响的交互作用。方法便利抽样选取2016年10月至2017年10月哈尔滨市某三级甲等医院NICU就诊的患儿家属193名,运用症状自评量表、应对方式问卷、家庭关怀度指数问卷对其进行调查。结果 NICU患儿家属心理健康得分为(156.00±71.96)分,高于全国常模;应对方式为(28.54±1.65)分,多采取不成熟的应对方式;家庭功能为(5.17±1.24)分,处于中度障碍水平。应对方式及家庭功能对患儿家属心理健康影响的主效应显著;经简单效应分析:家庭功能处于不同水平时,应对方式不同,患儿家属心理健康状况存在显著差异(P<0.01);在不成熟型应对方式下,家庭功能状况不同,患儿家属心理健康状况呈现显著差异(P<0.01)。结论应对方式及家庭功能的交互作用共同影响NICU患儿家属心理健康状况,应以两者交互关系为护理干预依据,进而有效改善患儿家属心理健康状况。展开更多
AIM： To investigate the effect of exogenous ceramideinduced apoptosis on human colon carcinoma HT-29 cells. METHODS： Light microscope, transmission electron microscope and fluorescence microscope were used to observ...AIM： To investigate the effect of exogenous ceramideinduced apoptosis on human colon carcinoma HT-29 cells. METHODS： Light microscope, transmission electron microscope and fluorescence microscope were used to observe the morphology change of apoptosis in HT-29 cells. Agarose gel electrophoresis was performed to detect the DNA fragment. Mitochondrial function was detected by MTT assay, mRNA expression of Bcl-2 family gene members was determined by reverse transcription polymerase chain reaction （RT-PCR） assay. RESULTS： After C2-ceramide treatment, typical characteristics of apoptosis, such as nuclear chromatin breakage, apoptotic body and DNA ladder, could be observed. After exposure to 50μmol/L C2-ceramide for 12 and 24 h, cell apoptosis was 64.1% and 81.3% respectively, which had a time-and dose-effect relationship. Mitochondrial function started to decrease from 6 h after exposure to ceramide. Meanwhile, ceramide up-regulated or down-regulated the mRNA expression of Bcl-2 family gene members. CONCLUSION： Ceramide induces apoptosis of human colon carcinoma HT-29 cells by affecting the expression of Bcl-2 family gene members and impacting the mitochondrial function.展开更多
文摘目的探讨应对方式及家庭功能对新生儿重症监护室(neonatal intensive care unit,NICU)患儿家属心理健康状况影响的交互作用。方法便利抽样选取2016年10月至2017年10月哈尔滨市某三级甲等医院NICU就诊的患儿家属193名,运用症状自评量表、应对方式问卷、家庭关怀度指数问卷对其进行调查。结果 NICU患儿家属心理健康得分为(156.00±71.96)分,高于全国常模;应对方式为(28.54±1.65)分,多采取不成熟的应对方式;家庭功能为(5.17±1.24)分,处于中度障碍水平。应对方式及家庭功能对患儿家属心理健康影响的主效应显著;经简单效应分析:家庭功能处于不同水平时,应对方式不同,患儿家属心理健康状况存在显著差异(P<0.01);在不成熟型应对方式下,家庭功能状况不同,患儿家属心理健康状况呈现显著差异(P<0.01)。结论应对方式及家庭功能的交互作用共同影响NICU患儿家属心理健康状况,应以两者交互关系为护理干预依据,进而有效改善患儿家属心理健康状况。
基金the National Natural Science Foundation of China, No. 30471447
文摘AIM： To investigate the effect of exogenous ceramideinduced apoptosis on human colon carcinoma HT-29 cells. METHODS： Light microscope, transmission electron microscope and fluorescence microscope were used to observe the morphology change of apoptosis in HT-29 cells. Agarose gel electrophoresis was performed to detect the DNA fragment. Mitochondrial function was detected by MTT assay, mRNA expression of Bcl-2 family gene members was determined by reverse transcription polymerase chain reaction （RT-PCR） assay. RESULTS： After C2-ceramide treatment, typical characteristics of apoptosis, such as nuclear chromatin breakage, apoptotic body and DNA ladder, could be observed. After exposure to 50μmol/L C2-ceramide for 12 and 24 h, cell apoptosis was 64.1% and 81.3% respectively, which had a time-and dose-effect relationship. Mitochondrial function started to decrease from 6 h after exposure to ceramide. Meanwhile, ceramide up-regulated or down-regulated the mRNA expression of Bcl-2 family gene members. CONCLUSION： Ceramide induces apoptosis of human colon carcinoma HT-29 cells by affecting the expression of Bcl-2 family gene members and impacting the mitochondrial function.
