Background Hypertensive intracerebral hemorrhage （HICH） is a severe disease with high morbidity and mortality. Timely removal of the hematoma through surgical procedures may effectively reduce secondary injuries. Ho...Background Hypertensive intracerebral hemorrhage （HICH） is a severe disease with high morbidity and mortality. Timely removal of the hematoma through surgical procedures may effectively reduce secondary injuries. However, there has long been a debate over the proper timing of such surgery. In this study, we explored the optimal operation time for HICH patients by observing the pathological changes in perihematomal brain regions during different stages of onset. Methods Twenty-five specimens of brain tissue, obtained from perihematomal region of HICH patients in different phases, were subjected to haematoxylin-eosin （HE） staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling （TUNEL） staining and Caspase-3, matrix metalloproteinases-9 （MMP-9） immunohistochemical staining. The changing roles of necrosis and apoptosis and the expression of MMP-9 and Caspase-3 positive cells were all observed using image analysis. Results The obvious expression of TUNEL positive cells was recognized within 6 hours of ICH onset, reaching its peak between 6 hours and 24 hours in the early phase. Results were highly consistent with Caspase-3 and MMP-9 positive cell counts. Necrosis was found 6 hours after ICH onset and aggravated after 12 hours. Conclusions In the early phase, apoptosis was seen as a major modality of injury in the brain tissue of the perihematomal region and was strongly correlated with the expression of MMP-9 and Caspase-3. The results of the present study suggest that an operation performed as soon as possible after ICH onset may be optimal for preserving the nervous system function.展开更多
AIM:To assess expression of matrix metalloproteinases 2(MMP2)and MMP9 in gastric cancer,superficial gastritis and normal mucosa,and to measure metalloproteinase activity.METHODS:MMP2 and MMP9 mRNA expression was deter...AIM:To assess expression of matrix metalloproteinases 2(MMP2)and MMP9 in gastric cancer,superficial gastritis and normal mucosa,and to measure metalloproteinase activity.METHODS:MMP2 and MMP9 mRNA expression was determined by quantitative real-time polymerase chain reaction.Normalization was carried out using three different factors.Proteins were analyzed by quantitative gelatin zymography(qGZ).RESULTS:18S ribosomal RNA(18SRNA)was very highly expressed,while hypoxanthine ribosyltransferase-1(HPRT-1)was moderately expressed.MMP2 was highly expressed,while MMP9 was not detected or lowly expressed in normal tissues,moderately or highly expressed in gastritis and highly expressed in cancer.Relative expression of 18SRNA and HPRT-1 showed no significant differences.Significant differences in MMP2 and MMP9 were found between cancer and normal tissue,but not between gastritis and normal tissue.Absolute quantification of MMP9 echoed this pattern,but differential expression of MMP2 proved conflictive.Analysis by qGZ indicated significant differences between cancer and normal tissue in MMP-2,total MMP-9,250 and 110 kDa bands.CONCLUSION:MMP9 expression is enhanced in gastric cancer compared to normal mucosa;interpretation of differential expression of MMP2 is difficult to establish.展开更多
目的观察盐酸法舒地尔注射液联合丁苯酞软胶囊治疗急性脑梗死14d的美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分改善及对血清基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶...目的观察盐酸法舒地尔注射液联合丁苯酞软胶囊治疗急性脑梗死14d的美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分改善及对血清基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)表达水平的影响。方法将78例急性脑梗死患者随机分为观察组和对照组各39例。对照组采用常规对症治疗,观察组在对照组治疗基础上加用盐酸法舒地尔注射液静脉滴注和丁苯酞软胶囊口服,2组均连续治疗14d。分别于治疗前后采用NIHSS评分对2组进行神经功能测定,并对治疗前后MMP-2、MMP-9水平进行比较。结果经过14d的治疗,观察组总有效率优于对照组(P<0.05),2组NIHSS评分及血清MMP-2、MMP-9水平均较治疗前显著降低(P<0.05),但观察组降低幅度更大,治疗后观察组NIHSS评分及血清MMP-2、MMP-9水平均低于对照组,差异有统计学意义(P<0.05)。结论盐酸法舒地尔注射液联合丁苯酞软胶囊可降低急性脑梗死患者的NIHSS评分,显著下调血清中MMP-2、MMP-9的表达。其机制可能与抑制炎症反应、减轻卒中后继发性脑损伤有关。展开更多
MMP-9 is one of the family of matrix metalloproteinases, which degrades extracellular matrix. MMP-9 is induced by many inflammatory factors, including IL-1β,IL-8 and TNF-α. Meanwhile, MMP-9 potentiates inflammatory ...MMP-9 is one of the family of matrix metalloproteinases, which degrades extracellular matrix. MMP-9 is induced by many inflammatory factors, including IL-1β,IL-8 and TNF-α. Meanwhile, MMP-9 potentiates inflammatory factors by aminoterminal processing. The signal transduction by which inflammatory factors induce MMP-9 is also an important part in recent research. This review will give a summary in all these fields.展开更多
文摘Background Hypertensive intracerebral hemorrhage （HICH） is a severe disease with high morbidity and mortality. Timely removal of the hematoma through surgical procedures may effectively reduce secondary injuries. However, there has long been a debate over the proper timing of such surgery. In this study, we explored the optimal operation time for HICH patients by observing the pathological changes in perihematomal brain regions during different stages of onset. Methods Twenty-five specimens of brain tissue, obtained from perihematomal region of HICH patients in different phases, were subjected to haematoxylin-eosin （HE） staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling （TUNEL） staining and Caspase-3, matrix metalloproteinases-9 （MMP-9） immunohistochemical staining. The changing roles of necrosis and apoptosis and the expression of MMP-9 and Caspase-3 positive cells were all observed using image analysis. Results The obvious expression of TUNEL positive cells was recognized within 6 hours of ICH onset, reaching its peak between 6 hours and 24 hours in the early phase. Results were highly consistent with Caspase-3 and MMP-9 positive cell counts. Necrosis was found 6 hours after ICH onset and aggravated after 12 hours. Conclusions In the early phase, apoptosis was seen as a major modality of injury in the brain tissue of the perihematomal region and was strongly correlated with the expression of MMP-9 and Caspase-3. The results of the present study suggest that an operation performed as soon as possible after ICH onset may be optimal for preserving the nervous system function.
基金Supported by The National Council on Science and Technology (CONACYT:85675 and 79628)Institute of Public Health(POA: 2008-2010)Research Office of Veracruzana University and Public Education Secretariat(SEP-PROMEP-UV:PTC-319)
文摘AIM:To assess expression of matrix metalloproteinases 2(MMP2)and MMP9 in gastric cancer,superficial gastritis and normal mucosa,and to measure metalloproteinase activity.METHODS:MMP2 and MMP9 mRNA expression was determined by quantitative real-time polymerase chain reaction.Normalization was carried out using three different factors.Proteins were analyzed by quantitative gelatin zymography(qGZ).RESULTS:18S ribosomal RNA(18SRNA)was very highly expressed,while hypoxanthine ribosyltransferase-1(HPRT-1)was moderately expressed.MMP2 was highly expressed,while MMP9 was not detected or lowly expressed in normal tissues,moderately or highly expressed in gastritis and highly expressed in cancer.Relative expression of 18SRNA and HPRT-1 showed no significant differences.Significant differences in MMP2 and MMP9 were found between cancer and normal tissue,but not between gastritis and normal tissue.Absolute quantification of MMP9 echoed this pattern,but differential expression of MMP2 proved conflictive.Analysis by qGZ indicated significant differences between cancer and normal tissue in MMP-2,total MMP-9,250 and 110 kDa bands.CONCLUSION:MMP9 expression is enhanced in gastric cancer compared to normal mucosa;interpretation of differential expression of MMP2 is difficult to establish.
文摘目的观察盐酸法舒地尔注射液联合丁苯酞软胶囊治疗急性脑梗死14d的美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分改善及对血清基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)表达水平的影响。方法将78例急性脑梗死患者随机分为观察组和对照组各39例。对照组采用常规对症治疗,观察组在对照组治疗基础上加用盐酸法舒地尔注射液静脉滴注和丁苯酞软胶囊口服,2组均连续治疗14d。分别于治疗前后采用NIHSS评分对2组进行神经功能测定,并对治疗前后MMP-2、MMP-9水平进行比较。结果经过14d的治疗,观察组总有效率优于对照组(P<0.05),2组NIHSS评分及血清MMP-2、MMP-9水平均较治疗前显著降低(P<0.05),但观察组降低幅度更大,治疗后观察组NIHSS评分及血清MMP-2、MMP-9水平均低于对照组,差异有统计学意义(P<0.05)。结论盐酸法舒地尔注射液联合丁苯酞软胶囊可降低急性脑梗死患者的NIHSS评分,显著下调血清中MMP-2、MMP-9的表达。其机制可能与抑制炎症反应、减轻卒中后继发性脑损伤有关。
文摘MMP-9 is one of the family of matrix metalloproteinases, which degrades extracellular matrix. MMP-9 is induced by many inflammatory factors, including IL-1β,IL-8 and TNF-α. Meanwhile, MMP-9 potentiates inflammatory factors by aminoterminal processing. The signal transduction by which inflammatory factors induce MMP-9 is also an important part in recent research. This review will give a summary in all these fields.
