Modified constraint-induced movement therapy is an effective treatment for neurological and motor impairments in patients with stroke by increasing the use of their affected limb and limiting the contralateral limb.Ho...Modified constraint-induced movement therapy is an effective treatment for neurological and motor impairments in patients with stroke by increasing the use of their affected limb and limiting the contralateral limb.However,the molecular mechanism underlying its efficacy remains unclear.In this study,a middle cerebral artery occlusion(MCAO)rat model was produced by the suture method.Rats received modified constraint-induced movement therapy 1 hour a day for 14 consecutive days,starting from the 7^th day after middle cerebral artery occlusion.Day 1 of treatment lasted for 10 minutes at 2r/min,day 2 for 20 minutes at 2 r/min,and from day 3 onward for 20 minutes at 4 r/min.CatWalk gait analysis,adhesive removal test,and Y-maze test were used to investigate motor function,sensory function as well as cognitive function in rodent animals from the 1st day before MCAO to the 21^st day after MCAO.On the 21^st day after MCAO,the neurotransmitter receptor-related genes from both contralateral and ipsilateral hippocampi were tested by micro-array and then verified by western blot assay.The glutamate related receptor was shown by transmission electron microscopy and the glutamate content was determined by high-performance liquid chromatography.The results of behavior tests showed that modified constraint-induced movement therapy promoted motor and sensory functional recovery in the middle cerebral artery-occluded rats,but had no effect on cognitive function.The modified constraint-induced movement therapy upregulated the expression of glutamate ionotropic receptor AMPA type subunit 3(Gria3)in the hippocampus and downregulated the expression of the beta3-adrenergic receptor gene Adrb3 and arginine vasopressin receptor 1 A,Avprla in the middle cerebral artery-occluded rats.In the ipsilateral hippocampus,only Adra2 a was downregulated,and there was no significant change in Gria3.Transmission electron microscopy revealed a denser distribution the more distribution of postsynaptic glutamate receptor 2/3,which is an a-amino-3-hydrox展开更多
目的分析临床中急性缺血性脑卒中伴有H型高血压患者血浆同型半胱氨酸(HCY)水平与颈动脉相关风险指标如颈动脉粥样斑块及颈动脉内膜中层厚度(CIMT)的相互关系。方法取本院收治的急性缺血性脑卒中合并H型高血压患者115例为A组,同期收治的...目的分析临床中急性缺血性脑卒中伴有H型高血压患者血浆同型半胱氨酸(HCY)水平与颈动脉相关风险指标如颈动脉粥样斑块及颈动脉内膜中层厚度(CIMT)的相互关系。方法取本院收治的急性缺血性脑卒中合并H型高血压患者115例为A组,同期收治的急性缺血性脑卒中合并单纯高血压患者68例为B组,同期收治的急性缺血性脑卒中患者110例为C组。测定3组患者的收缩压(SBP)、舒张压(DBP)、同型半胱氨酸(HCY)、血脂水平,检测其颈动脉斑块及CIMT,分析血压、HCY水平、血脂与颈动脉斑块及CIMT之间的相关性。结果三组间总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)比较,差异无统计学意义(P>0.05);A组SBP、DBP及甘油三酯(TG)高于B组和C组,而B组S B P、 D B P高于C组,两者差异均有统计学意义(P <0. 05); T G在B、 C两组中的分布未见明显差异(P>0. 05)。H C Y及CIMT值A组高于B、C两组(P <0.05),B、C两组间比较,差异无统计学意义(P>0.05)。3组患者斑块检测情况比较,差异有统计学意义(χ~2=13.346,P <0.05)。Pearson线性相关分析显示,急性缺血性脑卒中合并高血压患者的HCY水平与CIMT、斑块呈正相关(r=0.545,P <0.01)。结论急性缺血性脑卒中合并高血压患者的HCY水平与CIMT、颈动脉斑块形成等颈动脉风险指标呈正相关,HCY是CIMT增高与动脉粥样硬化斑块形成的独立危险因素。HCY及CIMT、颈动脉斑块的检测,对于评估和治疗急性缺血性脑卒中合并H型高血压患者具有重要的临床指导作用。展开更多
脑卒中后患者日常生活独立性的提高主要依赖运动功能[1],强制性使用运动疗法（constraint-induced movement therapy,CIMT）依据脑的可塑性与大脑功能重组理论,结合日常生活能力训练对解决脑卒中后患者运动功能障碍具有实际的临床应用价...脑卒中后患者日常生活独立性的提高主要依赖运动功能[1],强制性使用运动疗法（constraint-induced movement therapy,CIMT）依据脑的可塑性与大脑功能重组理论,结合日常生活能力训练对解决脑卒中后患者运动功能障碍具有实际的临床应用价值,近些年来此技术得以迅猛发展。此技术是通过限制健肢使用,强制患者使用患肢,集中强化并在日常生活中加以训练。展开更多
基金supported by the National Natural Science Foundation of China,No.81871841(to YLB) and No.81772453(to DSX)
文摘Modified constraint-induced movement therapy is an effective treatment for neurological and motor impairments in patients with stroke by increasing the use of their affected limb and limiting the contralateral limb.However,the molecular mechanism underlying its efficacy remains unclear.In this study,a middle cerebral artery occlusion(MCAO)rat model was produced by the suture method.Rats received modified constraint-induced movement therapy 1 hour a day for 14 consecutive days,starting from the 7^th day after middle cerebral artery occlusion.Day 1 of treatment lasted for 10 minutes at 2r/min,day 2 for 20 minutes at 2 r/min,and from day 3 onward for 20 minutes at 4 r/min.CatWalk gait analysis,adhesive removal test,and Y-maze test were used to investigate motor function,sensory function as well as cognitive function in rodent animals from the 1st day before MCAO to the 21^st day after MCAO.On the 21^st day after MCAO,the neurotransmitter receptor-related genes from both contralateral and ipsilateral hippocampi were tested by micro-array and then verified by western blot assay.The glutamate related receptor was shown by transmission electron microscopy and the glutamate content was determined by high-performance liquid chromatography.The results of behavior tests showed that modified constraint-induced movement therapy promoted motor and sensory functional recovery in the middle cerebral artery-occluded rats,but had no effect on cognitive function.The modified constraint-induced movement therapy upregulated the expression of glutamate ionotropic receptor AMPA type subunit 3(Gria3)in the hippocampus and downregulated the expression of the beta3-adrenergic receptor gene Adrb3 and arginine vasopressin receptor 1 A,Avprla in the middle cerebral artery-occluded rats.In the ipsilateral hippocampus,only Adra2 a was downregulated,and there was no significant change in Gria3.Transmission electron microscopy revealed a denser distribution the more distribution of postsynaptic glutamate receptor 2/3,which is an a-amino-3-hydrox
文摘目的分析临床中急性缺血性脑卒中伴有H型高血压患者血浆同型半胱氨酸(HCY)水平与颈动脉相关风险指标如颈动脉粥样斑块及颈动脉内膜中层厚度(CIMT)的相互关系。方法取本院收治的急性缺血性脑卒中合并H型高血压患者115例为A组,同期收治的急性缺血性脑卒中合并单纯高血压患者68例为B组,同期收治的急性缺血性脑卒中患者110例为C组。测定3组患者的收缩压(SBP)、舒张压(DBP)、同型半胱氨酸(HCY)、血脂水平,检测其颈动脉斑块及CIMT,分析血压、HCY水平、血脂与颈动脉斑块及CIMT之间的相关性。结果三组间总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)比较,差异无统计学意义(P>0.05);A组SBP、DBP及甘油三酯(TG)高于B组和C组,而B组S B P、 D B P高于C组,两者差异均有统计学意义(P <0. 05); T G在B、 C两组中的分布未见明显差异(P>0. 05)。H C Y及CIMT值A组高于B、C两组(P <0.05),B、C两组间比较,差异无统计学意义(P>0.05)。3组患者斑块检测情况比较,差异有统计学意义(χ~2=13.346,P <0.05)。Pearson线性相关分析显示,急性缺血性脑卒中合并高血压患者的HCY水平与CIMT、斑块呈正相关(r=0.545,P <0.01)。结论急性缺血性脑卒中合并高血压患者的HCY水平与CIMT、颈动脉斑块形成等颈动脉风险指标呈正相关,HCY是CIMT增高与动脉粥样硬化斑块形成的独立危险因素。HCY及CIMT、颈动脉斑块的检测,对于评估和治疗急性缺血性脑卒中合并H型高血压患者具有重要的临床指导作用。
文摘脑卒中后患者日常生活独立性的提高主要依赖运动功能[1],强制性使用运动疗法（constraint-induced movement therapy,CIMT）依据脑的可塑性与大脑功能重组理论,结合日常生活能力训练对解决脑卒中后患者运动功能障碍具有实际的临床应用价值,近些年来此技术得以迅猛发展。此技术是通过限制健肢使用,强制患者使用患肢,集中强化并在日常生活中加以训练。
