The application of nanotechnology for antimicrobial delivery has capacity to improve anti-bacterial efficacy.Currently,the usage of various inorganic and organic carriers,such as metal ions,nano-silicon and surfactant...The application of nanotechnology for antimicrobial delivery has capacity to improve anti-bacterial efficacy.Currently,the usage of various inorganic and organic carriers,such as metal ions,nano-silicon and surfactants,might increase the potential toxicity of nanoparticles and make their clinical transformation more difficult.Herein,a nano-delivery system was constructed by direct self-assembly of antibacterial phytochemicals(berberine and rhein)originated from traditional Chinese medicine Coptis chinensis Franch.and Rheum palmatum L.,respectively.Combining X-ray single crystal diffraction,nuclear magnetic resonance and other spectra characterizations,the stacked structure of nanoparticles was profoundly demonstrated.Briefly,rhein acted as the layered backbone and berberine embedded in it.In vitro bacteriostasis experiment showed the minimum bactericidal concentration of nanoparticles was 0.1μmol/mL,which was lower than that of berberine and rhein.The results of confocal laser scanning microscope,biofilm quantitive assay and scanning electron microscopy indicated that nanoparticles had strong inhibitory effects on Staphylococcus aureus biofilm.More importantly,transmission electron microscopy and mass spectra indicated the further bacteriostatic mechanism of nanoparticles.Meanwhile,the nanoparticles had well biocompatibility and safety.Current study will open up new prospect that the design of self-assemblies between active phytochemicals can be originated from traditional Chinese medicine combination.展开更多
基金funded by the Beijing Municipal Natural Science Foundation(No.7202116,China)National Natural Science Foundation of China(No.81603256)+2 种基金project of China Association of Chinese Medicine(CACM-2018-QNRC2-B08)the Fundamental Research Funds for the Central Universities(BUCM-2019-JCRC002,BUCM-2018-2020 and 2019-JYBTD005,China)Beijing Key Laboratory for Basic and Development Research on Chinese Medicine(Beijing,China)
文摘The application of nanotechnology for antimicrobial delivery has capacity to improve anti-bacterial efficacy.Currently,the usage of various inorganic and organic carriers,such as metal ions,nano-silicon and surfactants,might increase the potential toxicity of nanoparticles and make their clinical transformation more difficult.Herein,a nano-delivery system was constructed by direct self-assembly of antibacterial phytochemicals(berberine and rhein)originated from traditional Chinese medicine Coptis chinensis Franch.and Rheum palmatum L.,respectively.Combining X-ray single crystal diffraction,nuclear magnetic resonance and other spectra characterizations,the stacked structure of nanoparticles was profoundly demonstrated.Briefly,rhein acted as the layered backbone and berberine embedded in it.In vitro bacteriostasis experiment showed the minimum bactericidal concentration of nanoparticles was 0.1μmol/mL,which was lower than that of berberine and rhein.The results of confocal laser scanning microscope,biofilm quantitive assay and scanning electron microscopy indicated that nanoparticles had strong inhibitory effects on Staphylococcus aureus biofilm.More importantly,transmission electron microscopy and mass spectra indicated the further bacteriostatic mechanism of nanoparticles.Meanwhile,the nanoparticles had well biocompatibility and safety.Current study will open up new prospect that the design of self-assemblies between active phytochemicals can be originated from traditional Chinese medicine combination.
