In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis...In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 x 107 cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55-65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.展开更多
目的比较锁骨下臂丛神经阻滞定位臂丛后束与外侧束的阻滞成功率。方法选择70例择期行上肢手术的患者,随机分成外侧束组和后束组,采用 Wilson 提出的喙突入路,通过神经刺激器分别引发臂丛神经的外侧束或后束所支配的远端运动反应后给予0...目的比较锁骨下臂丛神经阻滞定位臂丛后束与外侧束的阻滞成功率。方法选择70例择期行上肢手术的患者,随机分成外侧束组和后束组,采用 Wilson 提出的喙突入路,通过神经刺激器分别引发臂丛神经的外侧束或后束所支配的远端运动反应后给予0.5%罗哌卡因30 ml。在给药后5 min、10 min、20 min、30 min 分别记录上肢5支终末神经(正中神经,肌皮神经,桡神经,尺神经,前臂内侧皮神经)的阻滞情况。结果外侧束组阻滞成功率为53.1%,后束组阻滞成功率为78.9%,后束组成功率显著高于外侧束组(P<0.05)。结论引发后束所支配的运动反应后给药,阻滞成功率要高于引发外侧束所支配的运动反应后给药的阻滞成功率。展开更多
基金the Science and Technology Foundation of Shenyang in China,No.F10-217-1-00
文摘In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 x 107 cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55-65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.
文摘目的比较锁骨下臂丛神经阻滞定位臂丛后束与外侧束的阻滞成功率。方法选择70例择期行上肢手术的患者,随机分成外侧束组和后束组,采用 Wilson 提出的喙突入路,通过神经刺激器分别引发臂丛神经的外侧束或后束所支配的远端运动反应后给予0.5%罗哌卡因30 ml。在给药后5 min、10 min、20 min、30 min 分别记录上肢5支终末神经(正中神经,肌皮神经,桡神经,尺神经,前臂内侧皮神经)的阻滞情况。结果外侧束组阻滞成功率为53.1%,后束组阻滞成功率为78.9%,后束组成功率显著高于外侧束组(P<0.05)。结论引发后束所支配的运动反应后给药,阻滞成功率要高于引发外侧束所支配的运动反应后给药的阻滞成功率。
