Autologous chondrocytes(C cells)are effective sources of cell therapy for engineering cartilage tissue to repair chondral defects,such as degenerative arthritis.The expansion of cells with C cell characteristics has b...Autologous chondrocytes(C cells)are effective sources of cell therapy for engineering cartilage tissue to repair chondral defects,such as degenerative arthritis.The expansion of cells with C cell characteristics has become a major challenge due to inadequate donor sites and poor proliferation of mature C cells.The perichondrial progenitor cells(P cells)from the cambium layer of the perichondrium possessed significantly higher mesenchymal stem cell markers than C cells.In the transwell co-culture system,P cells increased the passaging capacity of C cells from P6 to P9,and the cell number increased 128 times.This system increased the percentage of Alcian blue-positive C cells from 40%in P6 to 62%in P9,contributing about 198 times more Alcian blue-positive C cells than the control group.C cells co-cultured with P cells also exhibited higher proliferation than C cells cultured with P cellconditioned medium.Similar results were obtained in nude mice that were subcutaneously implanted with C cells,P cells or a mixture of the two cell types,in which the presence of both cells enhanced neocartilage formation in vivo.Inaggregate,P cells enhanced the proliferation of C cells in a dose–dependent manner and prolonged the longevity of mature C cells for clinical applications.展开更多
基金supported by grants from the Ministry of Science and Technology(MOST 109-2314-B-006-010-MY3)the National Science Council(NSC 93-2314-B-006-044,NSC 96-2314-B-006-047,NSC 97-2314-B-006-046-MY2,NSC 99-2314-B-006-013-MY2,NSC 99-3111-B-006-002)the National Cheng Kung University Medical Center(NCKUH-10401004),Tainan,Taiwan.
文摘Autologous chondrocytes(C cells)are effective sources of cell therapy for engineering cartilage tissue to repair chondral defects,such as degenerative arthritis.The expansion of cells with C cell characteristics has become a major challenge due to inadequate donor sites and poor proliferation of mature C cells.The perichondrial progenitor cells(P cells)from the cambium layer of the perichondrium possessed significantly higher mesenchymal stem cell markers than C cells.In the transwell co-culture system,P cells increased the passaging capacity of C cells from P6 to P9,and the cell number increased 128 times.This system increased the percentage of Alcian blue-positive C cells from 40%in P6 to 62%in P9,contributing about 198 times more Alcian blue-positive C cells than the control group.C cells co-cultured with P cells also exhibited higher proliferation than C cells cultured with P cellconditioned medium.Similar results were obtained in nude mice that were subcutaneously implanted with C cells,P cells or a mixture of the two cell types,in which the presence of both cells enhanced neocartilage formation in vivo.Inaggregate,P cells enhanced the proliferation of C cells in a dose–dependent manner and prolonged the longevity of mature C cells for clinical applications.
