OBJECTIVE: To evaluate the efficacy of α-lipoic acid(ALA) plus epalrestat combination therapy in the treatment of diabetic peripheral neuropathy(DPN). DATA SOURCES: The electronic databases of Pub Med, Medline,...OBJECTIVE: To evaluate the efficacy of α-lipoic acid(ALA) plus epalrestat combination therapy in the treatment of diabetic peripheral neuropathy(DPN). DATA SOURCES: The electronic databases of Pub Med, Medline, Embase, the Cochrane Library, the Chinese National Knowledge Infrastructure, the Wanfang Database and the Chinese Biomedical Database were used to retrieve relevant studies without language restrictions. The search was conducted from the inception of each database to 7 October 2016. The key terms were(diabetic peripheral neuropathy or diabetic neuropathy or DPN) AND(α-lipoic acid or lipoic acid or thioctic acid) AND epalrestat. DATA SELECTION: All of the eligible studies met the following inclusion criteria:(1) Randomized controlled trials that compared efficacy and safety of epalrestat plus ALA combination therapy versus epalrestat or ALA monotherapy in patients with DPN.(2) The minimum duration of treatment was 2 weeks.(3) The DPN patients were diagnosed using the World Health Organization standardized type 2 diabetes mellitus and DPN criteria.(4) Studies contained at least one measure that could reflect the efficacy of the drug and nerve conduction velocities. Studies in which the control group used epalrestat or ALA combined with other drugs were excluded. Statistical analyses were performed using STATA software for meta-analysis. OUTCOME MEASURES: The primary outcomes were the therapeutic efficacy, median motor nerve conduction velocity(MNCV), median sensory nerve conduction velocity(SNCV), peroneal MNCV and peroneal SNCV.RESULTS: Twenty studies with 1894 DPN patients were included, including 864 patients in the ALA plus epalrestat group, 473 in the ALA group and 557 in the epalrestat group. The efficacy of ALA plus epalrestat combination therapy was superior to ALA and epalrestat monotherapies(RR = 1.29, 95% CI: 1.21–1.38; RR = 1.43, 95% CI: 1.34–1.54, respectively). ALA plus epalrestat combination therapy also significantly improved m展开更多
In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis...In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 x 107 cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55-65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.展开更多
目的探讨超声引导及神经电生理监测下锁骨上臂丛神经阻滞的最小有效剂量。方法选取240例前壁闭合骨折患者为研究对象,均采用切开复位内固定术治疗,随机均分为L1、L2、R1、R2四组,均在超声引导及神经电生理监测下,行患侧锁骨上臂丛神经阻...目的探讨超声引导及神经电生理监测下锁骨上臂丛神经阻滞的最小有效剂量。方法选取240例前壁闭合骨折患者为研究对象,均采用切开复位内固定术治疗,随机均分为L1、L2、R1、R2四组,均在超声引导及神经电生理监测下,行患侧锁骨上臂丛神经阻滞,L1组、L2组分别应用0.25%、0.375%的左布比卡因,R1组、R2组分别应用0.25%、0.375%的罗哌卡因,均采用序贯实验法审定给药计量,每个剂量阶梯之间相差1.0 m L,观察不同剂量下阻滞起效时间、阻滞成功率。结果 0.25%左布比卡因ED_(50)为17.8 m L,0.375%左布比卡因ED_(50)为17.0 m L,0.25%罗哌卡因ED_(50)为18.5 m L,0.375%罗哌卡因ED_(50)为17.4 m L。结论测定超声引导下左布比卡因和罗哌卡因锁骨上神经阻滞最小有效剂量,对精确和规范用药有重要的临床指导意义。展开更多
Artificial facial nerve prostheses are thought to restore eye-closed function in peripheral facial paralysis patients.At present,however,there is no adequate quantitative or qualitative information regarding myoelectr...Artificial facial nerve prostheses are thought to restore eye-closed function in peripheral facial paralysis patients.At present,however,there is no adequate quantitative or qualitative information regarding myoelectric signal(MES)features for healthy orbiculads oculi muscle(OOM).The present study analyzed MES features of normal OOM in rabbits during the natural continuous eye-opening(N1)state,natural continuous eye-closing(N2)state,natural blink(N3)state,and evoked eye-closing(E)state according to time domain and frequency domain analysis.Results showed that OOM electrical activities in N1 and N2 states,as well as myoelectric amplitude,were low and stable.Nevertheless,during N3 and E states,OOM electrical activities were significantly increased and amplitude was much higher in the E state than in the N3 state.In the time domain,differences in MES peak absolute potential were not significant between N1 and N2 states,in the frequency domain,differences in power spectral density peak frequency of electromyogram signals were significant between two sets of four OOM movement states.These results suggest that OOM significantly contracts and induces eyelid-closing action.In addition,OOM is diastolic during the N1state.A N2 state does not require continuous intensive OOM contraction.Moreover,distinctions of quantitative information in time and frequency domain features of MES can be used as an OOM reference to identify muscle movement patterns.展开更多
A total of 43 prolonged coma patients with diffuse axonal injury received the somatosensory evoked potential examination one month after injury in the First Affiliated Hospital, School of Medicine, Zhejiang University...A total of 43 prolonged coma patients with diffuse axonal injury received the somatosensory evoked potential examination one month after injury in the First Affiliated Hospital, School of Medicine, Zhejiang University in China. Somatosensory evoked potentials were graded as normal, abnormal or absent (grades I-III) according to N20 amplitude and central conduction time. The outcome in patients with grade III somatosensory evoked potential was in each case unfavorable. The prognostic accuracy of grade III somatosensory evoked potential for unfavorable and non-awakening outcome was 100% and 80%, respectively. The prognostic accuracy of grade I somatosensory evoked potential for favorable and wakening outcome was 86% and 100%, respectively. These results suggest that somatosensory evoked potential grade is closely correlated with coma severity and degree of recovery. Somatosensory evoked potential is a valuable diagnostic tool to assess prognosis in prolonged coma patients with diffuse axonal injury.展开更多
基金supported by the National Natural Science Foundation of China,No.81370165a grant from the Public Benefit Technology and Society Development Program of Zhejiang Province of China,No.2015C33309+2 种基金a grant from the Ningbo Science and Technology Innovation Team Program in China,No.2014B82002,2015B11050a grant from the Ningbo Science and Technology Project in China,No.2015A610217the Fang Runhua Fund of Hong Kong,K.C.Wong Magna Fund in Ningbo University
文摘OBJECTIVE: To evaluate the efficacy of α-lipoic acid(ALA) plus epalrestat combination therapy in the treatment of diabetic peripheral neuropathy(DPN). DATA SOURCES: The electronic databases of Pub Med, Medline, Embase, the Cochrane Library, the Chinese National Knowledge Infrastructure, the Wanfang Database and the Chinese Biomedical Database were used to retrieve relevant studies without language restrictions. The search was conducted from the inception of each database to 7 October 2016. The key terms were(diabetic peripheral neuropathy or diabetic neuropathy or DPN) AND(α-lipoic acid or lipoic acid or thioctic acid) AND epalrestat. DATA SELECTION: All of the eligible studies met the following inclusion criteria:(1) Randomized controlled trials that compared efficacy and safety of epalrestat plus ALA combination therapy versus epalrestat or ALA monotherapy in patients with DPN.(2) The minimum duration of treatment was 2 weeks.(3) The DPN patients were diagnosed using the World Health Organization standardized type 2 diabetes mellitus and DPN criteria.(4) Studies contained at least one measure that could reflect the efficacy of the drug and nerve conduction velocities. Studies in which the control group used epalrestat or ALA combined with other drugs were excluded. Statistical analyses were performed using STATA software for meta-analysis. OUTCOME MEASURES: The primary outcomes were the therapeutic efficacy, median motor nerve conduction velocity(MNCV), median sensory nerve conduction velocity(SNCV), peroneal MNCV and peroneal SNCV.RESULTS: Twenty studies with 1894 DPN patients were included, including 864 patients in the ALA plus epalrestat group, 473 in the ALA group and 557 in the epalrestat group. The efficacy of ALA plus epalrestat combination therapy was superior to ALA and epalrestat monotherapies(RR = 1.29, 95% CI: 1.21–1.38; RR = 1.43, 95% CI: 1.34–1.54, respectively). ALA plus epalrestat combination therapy also significantly improved m
基金the Science and Technology Foundation of Shenyang in China,No.F10-217-1-00
文摘In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 x 107 cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55-65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.
文摘目的探讨超声引导及神经电生理监测下锁骨上臂丛神经阻滞的最小有效剂量。方法选取240例前壁闭合骨折患者为研究对象,均采用切开复位内固定术治疗,随机均分为L1、L2、R1、R2四组,均在超声引导及神经电生理监测下,行患侧锁骨上臂丛神经阻滞,L1组、L2组分别应用0.25%、0.375%的左布比卡因,R1组、R2组分别应用0.25%、0.375%的罗哌卡因,均采用序贯实验法审定给药计量,每个剂量阶梯之间相差1.0 m L,观察不同剂量下阻滞起效时间、阻滞成功率。结果 0.25%左布比卡因ED_(50)为17.8 m L,0.375%左布比卡因ED_(50)为17.0 m L,0.25%罗哌卡因ED_(50)为18.5 m L,0.375%罗哌卡因ED_(50)为17.4 m L。结论测定超声引导下左布比卡因和罗哌卡因锁骨上神经阻滞最小有效剂量,对精确和规范用药有重要的临床指导意义。
基金the National Natural Science Foundation of China,No.60876082,81070779the grant from Shanghai Committee of Science and Technology,No.0852nm06600the "Shu Guang" Project supported by Shanghai Municipal Educa-tion Commission and Shanghai Education Devel-opment Foundation,No.08SG13
文摘Artificial facial nerve prostheses are thought to restore eye-closed function in peripheral facial paralysis patients.At present,however,there is no adequate quantitative or qualitative information regarding myoelectric signal(MES)features for healthy orbiculads oculi muscle(OOM).The present study analyzed MES features of normal OOM in rabbits during the natural continuous eye-opening(N1)state,natural continuous eye-closing(N2)state,natural blink(N3)state,and evoked eye-closing(E)state according to time domain and frequency domain analysis.Results showed that OOM electrical activities in N1 and N2 states,as well as myoelectric amplitude,were low and stable.Nevertheless,during N3 and E states,OOM electrical activities were significantly increased and amplitude was much higher in the E state than in the N3 state.In the time domain,differences in MES peak absolute potential were not significant between N1 and N2 states,in the frequency domain,differences in power spectral density peak frequency of electromyogram signals were significant between two sets of four OOM movement states.These results suggest that OOM significantly contracts and induces eyelid-closing action.In addition,OOM is diastolic during the N1state.A N2 state does not require continuous intensive OOM contraction.Moreover,distinctions of quantitative information in time and frequency domain features of MES can be used as an OOM reference to identify muscle movement patterns.
基金funded by Zhejiang Medicines &Health Sciences Research Fund (Class A) in 2009, No.2009A086
文摘A total of 43 prolonged coma patients with diffuse axonal injury received the somatosensory evoked potential examination one month after injury in the First Affiliated Hospital, School of Medicine, Zhejiang University in China. Somatosensory evoked potentials were graded as normal, abnormal or absent (grades I-III) according to N20 amplitude and central conduction time. The outcome in patients with grade III somatosensory evoked potential was in each case unfavorable. The prognostic accuracy of grade III somatosensory evoked potential for unfavorable and non-awakening outcome was 100% and 80%, respectively. The prognostic accuracy of grade I somatosensory evoked potential for favorable and wakening outcome was 86% and 100%, respectively. These results suggest that somatosensory evoked potential grade is closely correlated with coma severity and degree of recovery. Somatosensory evoked potential is a valuable diagnostic tool to assess prognosis in prolonged coma patients with diffuse axonal injury.