目的探讨强直性脊柱炎（ankylosing spondylitis，AS）患者外周血中CD4^＋调节性T细胞（regulatory T cells，Treg）的表达、功能及意义。方法采用流式细胞术检测78例As患者和50例健康志愿者外周血中CD4^＋CD25^＋CD127^lo/-Treg、细胞...目的探讨强直性脊柱炎（ankylosing spondylitis，AS）患者外周血中CD4^＋调节性T细胞（regulatory T cells，Treg）的表达、功能及意义。方法采用流式细胞术检测78例As患者和50例健康志愿者外周血中CD4^＋CD25^＋CD127^lo/-Treg、细胞毒性T细胞（cytotoxic T lymphocytes，CTL）和NK细胞，采用ELISA法检测血清中B型转化生长因子（transforming growth factor-β，TGF-β）和肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的表达水平。从患者外周血单个核细胞中磁珠分选出CD4^＋CD25^＋Treg细胞，混合淋巴细胞培养（mixedlymphocyteculture，MLC）分析其免疫抑制功能。结果AS活动期患者外周血中CD4^＋CD25^＋CD127^lo-Treg占CD4^＋T淋巴细胞的百分比为（4．36±1．21）％，MLC中CD4^＋CD25^＋Treg抑制同种异体T淋巴细胞增殖功能低下，与其Treg分泌TGF-β减少相关，CD4^＋Treg含量及功能均低于健康志愿者（P〈0．05）。AS患者外周血中CD4^＋CD25^＋CD127^lo/-Treg水平与TGF—β呈正相关，与TNF-α呈负相关。结论AS患者外周血中Treg表达水平低，且存在功能缺陷，导致体内诱导免疫耐受机能不足，可能参与AS免疫发病。展开更多
The main approach to reduce graft rejection has been focused on the development of immunosuppressive agents at present.Although these strategies have reportedly reduced graft rejection,there has been a reciprocal incr...The main approach to reduce graft rejection has been focused on the development of immunosuppressive agents at present.Although these strategies have reportedly reduced graft rejection,there has been a reciprocal increase in more severe immunosuppression and lethal infections,as well as severe side effects.Blockade of costimulatory T cell response has been proved as one of useful strategies to reduce graft rejection.Furthermore, it has been shown that infusion of dendritic cells(DCs)with a potent negative regulatory ability for T cells could prolong allograft survival.In this study mouse DCs(mDCs)were transfected with the recombinant plasmid pcDNA3.0 containing mouse inducible costimulator-Ig(mICOS-Ig) cDNA by electroporation.The transient expression of mICOS-Ig in mDC could be detected by ELISA and SDS-PAGE.Mouse ICOS-Ig fusion protein expressed in mDC and mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in mixed lymphocyte culture(MLC)in vitro.Furthermore,mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in recipient mice.These results suggested that mICOS-Ig gene-modified mDC exerted inhibitory effects on T cells,and might be suitable for treatment or prevention of graft rejection and immunopathologic diseases.Cellular & Molecular Immunology.2004;1(2):153-157.展开更多
文摘目的探讨强直性脊柱炎（ankylosing spondylitis，AS）患者外周血中CD4^＋调节性T细胞（regulatory T cells，Treg）的表达、功能及意义。方法采用流式细胞术检测78例As患者和50例健康志愿者外周血中CD4^＋CD25^＋CD127^lo/-Treg、细胞毒性T细胞（cytotoxic T lymphocytes，CTL）和NK细胞，采用ELISA法检测血清中B型转化生长因子（transforming growth factor-β，TGF-β）和肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的表达水平。从患者外周血单个核细胞中磁珠分选出CD4^＋CD25^＋Treg细胞，混合淋巴细胞培养（mixedlymphocyteculture，MLC）分析其免疫抑制功能。结果AS活动期患者外周血中CD4^＋CD25^＋CD127^lo-Treg占CD4^＋T淋巴细胞的百分比为（4．36±1．21）％，MLC中CD4^＋CD25^＋Treg抑制同种异体T淋巴细胞增殖功能低下，与其Treg分泌TGF-β减少相关，CD4^＋Treg含量及功能均低于健康志愿者（P〈0．05）。AS患者外周血中CD4^＋CD25^＋CD127^lo/-Treg水平与TGF—β呈正相关，与TNF-α呈负相关。结论AS患者外周血中Treg表达水平低，且存在功能缺陷，导致体内诱导免疫耐受机能不足，可能参与AS免疫发病。
基金surpported by National Key Basic Research Program of China(No.CB510008)
文摘The main approach to reduce graft rejection has been focused on the development of immunosuppressive agents at present.Although these strategies have reportedly reduced graft rejection,there has been a reciprocal increase in more severe immunosuppression and lethal infections,as well as severe side effects.Blockade of costimulatory T cell response has been proved as one of useful strategies to reduce graft rejection.Furthermore, it has been shown that infusion of dendritic cells(DCs)with a potent negative regulatory ability for T cells could prolong allograft survival.In this study mouse DCs(mDCs)were transfected with the recombinant plasmid pcDNA3.0 containing mouse inducible costimulator-Ig(mICOS-Ig) cDNA by electroporation.The transient expression of mICOS-Ig in mDC could be detected by ELISA and SDS-PAGE.Mouse ICOS-Ig fusion protein expressed in mDC and mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in mixed lymphocyte culture(MLC)in vitro.Furthermore,mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in recipient mice.These results suggested that mICOS-Ig gene-modified mDC exerted inhibitory effects on T cells,and might be suitable for treatment or prevention of graft rejection and immunopathologic diseases.Cellular & Molecular Immunology.2004;1(2):153-157.
