The generation of a recombinant HSV (rHSV) that can provide packaging function for rAAV production is described. A set of cosmids including cos48, cos28, cos6, cos14 and cos56, which represents the HSV-1 genome was us...The generation of a recombinant HSV (rHSV) that can provide packaging function for rAAV production is described. A set of cosmids including cos48, cos28, cos6, cos14 and cos56, which represents the HSV-1 genome was used for generation of this rHSV. Rep and cap genes of AAV-2 were inserted into Xba Ⅰ site of UL2 gene on cos6, generating cos6-rcΔUL2. After being digested with Pac Ⅰ , cos6-rcΔUL2 and the other 4 cosmids were cotrans-fected into BHK-21 cells. The recombinant virus HSV1-rc/ΔUL2 carrying rep and cap genes was generated due to the homologous recombination of the 5 cosmids. The results showed that the existence of rep and cap genes on this rHSV was stable from passage to passage and the rHSV could support the packaging of rAAV either in cells transiently transfected with AAV vector or in stable cell line harboring AAV vector. Further modification of this rHSV and optimization of conditions involved in rAAV preparation may lead to a large-scale production of rAAV in the near future.展开更多
In this paper, recent measurements of tip vortex flow with and without cavitation carried out in Cavitation Mechanism Tunnel of China Ship Scientific Research Center（CSSRC） are presented. The elliptic hydrofoil with...In this paper, recent measurements of tip vortex flow with and without cavitation carried out in Cavitation Mechanism Tunnel of China Ship Scientific Research Center（CSSRC） are presented. The elliptic hydrofoil with section NACA 662-415 was adopted as test model. High-speed video（HSV） camera was used to visualize the trajectory of tip vortex core and the form of tip vortex cavitation（TVC） in different cavitation situations. Laser Doppler velocimetry（LDV） was employed to measure the tip vortex flow field in some typical sections along the vortex trajectory with the case of cavitation free. Stereo particle image velocimetry（SPIV） system was used to measure the velocity and vorticity distributions with and without cavitation. Series measurement results such as velocity and vorticity distributions, the trajectory of tip vortex core, the vortex core radius, cavity size and cavitation inception number were obtained. The results demonstrated that the minimum pressure coefficient in the vortex core obtained by flow field measurement was quite coincident with the tip vortex cavitation inception number obtained under the condition of high incoming velocity and low air content. And TVC would decrease the vortex strength comparing with the case without cavitation.展开更多
文摘The generation of a recombinant HSV (rHSV) that can provide packaging function for rAAV production is described. A set of cosmids including cos48, cos28, cos6, cos14 and cos56, which represents the HSV-1 genome was used for generation of this rHSV. Rep and cap genes of AAV-2 were inserted into Xba Ⅰ site of UL2 gene on cos6, generating cos6-rcΔUL2. After being digested with Pac Ⅰ , cos6-rcΔUL2 and the other 4 cosmids were cotrans-fected into BHK-21 cells. The recombinant virus HSV1-rc/ΔUL2 carrying rep and cap genes was generated due to the homologous recombination of the 5 cosmids. The results showed that the existence of rep and cap genes on this rHSV was stable from passage to passage and the rHSV could support the packaging of rAAV either in cells transiently transfected with AAV vector or in stable cell line harboring AAV vector. Further modification of this rHSV and optimization of conditions involved in rAAV preparation may lead to a large-scale production of rAAV in the near future.
基金Project supported by the Key Project of National Natural Science Foundation of China(Grant No.11332009)
文摘In this paper, recent measurements of tip vortex flow with and without cavitation carried out in Cavitation Mechanism Tunnel of China Ship Scientific Research Center（CSSRC） are presented. The elliptic hydrofoil with section NACA 662-415 was adopted as test model. High-speed video（HSV） camera was used to visualize the trajectory of tip vortex core and the form of tip vortex cavitation（TVC） in different cavitation situations. Laser Doppler velocimetry（LDV） was employed to measure the tip vortex flow field in some typical sections along the vortex trajectory with the case of cavitation free. Stereo particle image velocimetry（SPIV） system was used to measure the velocity and vorticity distributions with and without cavitation. Series measurement results such as velocity and vorticity distributions, the trajectory of tip vortex core, the vortex core radius, cavity size and cavitation inception number were obtained. The results demonstrated that the minimum pressure coefficient in the vortex core obtained by flow field measurement was quite coincident with the tip vortex cavitation inception number obtained under the condition of high incoming velocity and low air content. And TVC would decrease the vortex strength comparing with the case without cavitation.
