Objective To identify the antimicrobial resistance of commercial lactic acid bacteria present in microbial foods and drug additives by analyzing their isolated strains used for fermentation and probiotics. Methods Ant...Objective To identify the antimicrobial resistance of commercial lactic acid bacteria present in microbial foods and drug additives by analyzing their isolated strains used for fermentation and probiotics. Methods Antimicrobial susceptibility of 41 screened isolates was tested with disc diffusion and E-test methods after species-level identification. Resistant strains were selected and examined for the presence of resistance genes by PCR. Results Distribution of resistance was found in different species. All isolates were susceptible to chloramphenicol, tetracycline, ampicillin, amoxicillin/clavulanic acid, cephalothin, and imipenem. In addition, isolates resistant to vancomycin, rifampicin, streptomycin, bacitracin, and erythromycin were detected, although the incidence of resistance to these antibiotics was relatively low. In contrast, most strains were resistant to ciprofloxacin, amikacin, trimethoprim/sulphamethoxazole, and gentamycin. The genes msrC, vanX, and dfrA were detected in strains of Enterococcus faecium, Lactobacillus plantarum, Streptococcus thermophilus, and Lactococcus lactis. Conclusion Antibiotic resistance is present in different species of probiotic strains, which poses a threat to food safety. Evaluation of the safety of lactic acid bacteria for human consumption should be guided by established criteria, guidelines and regulations.展开更多
Objective:To screen the antibacterial activity of nine ethnoveterinary plants traditionally used for the treatment of mastitis,wound and gastrointestinal complications.Methods:Hydroalcoholic exctracts of medicinal pla...Objective:To screen the antibacterial activity of nine ethnoveterinary plants traditionally used for the treatment of mastitis,wound and gastrointestinal complications.Methods:Hydroalcoholic exctracts of medicinal plants namely,Achyranthes aspera(A.aspera) L.(Family Asparagaceae),Ficus caria(F.caria)(Family Moraceae).Malvi parviflora(M.parviflora)(Family Malvaceae),Vernonia species(V.species) docal name Alakit.Family Asteraceae),Solatium hastifolium(S.hastifolium)(Family Solanaceae),Calpurinia aurea(C.aurea)(Ait) Benth(Family Fabaceae),Nicotiana tabacum(N.tabacum) L.(Family Solanaceae),Ziziphus spina-christi(Z.spina-christi)(Family Rhamnaceae).Croton macroslachys(C.macrostachys)(Family Fuphorhiaceae).were screened against clinical bacterial isolates of veterinary importance from October 2007 to April2009.The antibacterial activity was tested using disc diffusion at two concentrations(200 mg/mL and 100 mg/mL) and broth dilution methods using 70%methanol macerated leal extracts.Results:With the exception ol S.hastifolium all plant extracts exhibited antibacterial activity.Among the medicinal plants tested C.aurea.C.macrostachyus,A.aspera,N.tabacum and vcrnonia species(Alakit) showed the most promising antimicrobial properties.Conclusions:It can be concluded that many of the tested plants have antibacterial activity and supports the traditional usage of the plants for mastitis,wound and gastrointestinal complications treatment.Further studies into their toxicity and phytochemistry is advocated.展开更多
<b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study w...<b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study was to isolate, identify, and explore the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antifungal susceptibility pattern of dermatophytes isolated from clinica</span><span style="font-family:Verdana;">lly suspected cases of dermatophytosis (tinea infections) attending th</span><span style="font-family:Verdana;">e Dermatology Department at J.S.S Hospital. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">This study was conducted at JSS Medical College and Hospital from December 2016 to December 2017. Clinical samples (e.g., skin scrapings and hair stumps) were collected under asep</span><span style="font-family:Verdana;">tic precautions. The identification of dermatophytes was perfor</span><span style="font-family:Verdana;">med</span><span style="font-family:Verdana;"> through microscopic examination using 10%, 20% & 40% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar (SDA), SDAac, PDA and Dermatophyte test medium (DTM). All dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-base</span><span style="font-family:Verdana;">d disk diffusion (ABDD) and E-test method against Terbinafine, Itraconazole, Fluconazole, and Griseofulvin. Data were analyzed by using Chi square test. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A 100 samples were studied, 46% tinea corporis, 2%tinea cruris, 9% tinea pedis, 5% tin</span><span><span style="font-family:Verdana;">ea faciei. The dermatophytes isolated were </span><i><span style="font-family:Verdana;">Trichophyton rubrum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">1 (35%), </span><i><span 展开更多
文摘Objective To identify the antimicrobial resistance of commercial lactic acid bacteria present in microbial foods and drug additives by analyzing their isolated strains used for fermentation and probiotics. Methods Antimicrobial susceptibility of 41 screened isolates was tested with disc diffusion and E-test methods after species-level identification. Resistant strains were selected and examined for the presence of resistance genes by PCR. Results Distribution of resistance was found in different species. All isolates were susceptible to chloramphenicol, tetracycline, ampicillin, amoxicillin/clavulanic acid, cephalothin, and imipenem. In addition, isolates resistant to vancomycin, rifampicin, streptomycin, bacitracin, and erythromycin were detected, although the incidence of resistance to these antibiotics was relatively low. In contrast, most strains were resistant to ciprofloxacin, amikacin, trimethoprim/sulphamethoxazole, and gentamycin. The genes msrC, vanX, and dfrA were detected in strains of Enterococcus faecium, Lactobacillus plantarum, Streptococcus thermophilus, and Lactococcus lactis. Conclusion Antibiotic resistance is present in different species of probiotic strains, which poses a threat to food safety. Evaluation of the safety of lactic acid bacteria for human consumption should be guided by established criteria, guidelines and regulations.
基金Ethiopian Ministry of Science and Technology research and development grant FVS/11/2008
文摘Objective:To screen the antibacterial activity of nine ethnoveterinary plants traditionally used for the treatment of mastitis,wound and gastrointestinal complications.Methods:Hydroalcoholic exctracts of medicinal plants namely,Achyranthes aspera(A.aspera) L.(Family Asparagaceae),Ficus caria(F.caria)(Family Moraceae).Malvi parviflora(M.parviflora)(Family Malvaceae),Vernonia species(V.species) docal name Alakit.Family Asteraceae),Solatium hastifolium(S.hastifolium)(Family Solanaceae),Calpurinia aurea(C.aurea)(Ait) Benth(Family Fabaceae),Nicotiana tabacum(N.tabacum) L.(Family Solanaceae),Ziziphus spina-christi(Z.spina-christi)(Family Rhamnaceae).Croton macroslachys(C.macrostachys)(Family Fuphorhiaceae).were screened against clinical bacterial isolates of veterinary importance from October 2007 to April2009.The antibacterial activity was tested using disc diffusion at two concentrations(200 mg/mL and 100 mg/mL) and broth dilution methods using 70%methanol macerated leal extracts.Results:With the exception ol S.hastifolium all plant extracts exhibited antibacterial activity.Among the medicinal plants tested C.aurea.C.macrostachyus,A.aspera,N.tabacum and vcrnonia species(Alakit) showed the most promising antimicrobial properties.Conclusions:It can be concluded that many of the tested plants have antibacterial activity and supports the traditional usage of the plants for mastitis,wound and gastrointestinal complications treatment.Further studies into their toxicity and phytochemistry is advocated.
文摘<b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study was to isolate, identify, and explore the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antifungal susceptibility pattern of dermatophytes isolated from clinica</span><span style="font-family:Verdana;">lly suspected cases of dermatophytosis (tinea infections) attending th</span><span style="font-family:Verdana;">e Dermatology Department at J.S.S Hospital. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">This study was conducted at JSS Medical College and Hospital from December 2016 to December 2017. Clinical samples (e.g., skin scrapings and hair stumps) were collected under asep</span><span style="font-family:Verdana;">tic precautions. The identification of dermatophytes was perfor</span><span style="font-family:Verdana;">med</span><span style="font-family:Verdana;"> through microscopic examination using 10%, 20% & 40% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar (SDA), SDAac, PDA and Dermatophyte test medium (DTM). All dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-base</span><span style="font-family:Verdana;">d disk diffusion (ABDD) and E-test method against Terbinafine, Itraconazole, Fluconazole, and Griseofulvin. Data were analyzed by using Chi square test. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A 100 samples were studied, 46% tinea corporis, 2%tinea cruris, 9% tinea pedis, 5% tin</span><span><span style="font-family:Verdana;">ea faciei. The dermatophytes isolated were </span><i><span style="font-family:Verdana;">Trichophyton rubrum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">1 (35%), </span><i><span
