Microscopic examination of Spodoptera litura(SL-1)cell infected with AcMNPV or SfaMNPV revealed progressive cell blebbing starting at 8h～12h postinfection and culminating in total cell destruction at 24h postinfectio...Microscopic examination of Spodoptera litura(SL-1)cell infected with AcMNPV or SfaMNPV revealed progressive cell blebbing starting at 8h～12h postinfection and culminating in total cell destruction at 24h postinfection.The fragmentation of the infected cell nuclei was observed by stained with the specific fluorescent dye DAPI.Agarose gel electrophoresis analysis of the DNA extracted from infected cells show typical DNA ladder. All these supported that both viruses infected SL-1 cells undergo apoptosis.Through lipofectin transinfection,we observed that only the DNA of AcMNPV or SfaMNPV can also induce apopotosis of SL-1 cell.Virus titer analysis revealed that neither viruses can duplicate in SL-1 cells.展开更多
The crucial role of cell death in many diseases is obvious and has intense researches to understand the regulation of apoptotic pathways. Caspase activation is central to many of the apoptotic pathways. In recent year...The crucial role of cell death in many diseases is obvious and has intense researches to understand the regulation of apoptotic pathways. Caspase activation is central to many of the apoptotic pathways. In recent years, the research on the regulation and activation of caspase has made a great progress. Caspase inhibitors prevent active caspases from committing the cell to irreversible destruction. This review will mainly focus on the characteristics of natural anticaspase inhibitor-IAP, regulation of IAP expression, mechanisms of action of IAP.展开更多
文摘Microscopic examination of Spodoptera litura(SL-1)cell infected with AcMNPV or SfaMNPV revealed progressive cell blebbing starting at 8h～12h postinfection and culminating in total cell destruction at 24h postinfection.The fragmentation of the infected cell nuclei was observed by stained with the specific fluorescent dye DAPI.Agarose gel electrophoresis analysis of the DNA extracted from infected cells show typical DNA ladder. All these supported that both viruses infected SL-1 cells undergo apoptosis.Through lipofectin transinfection,we observed that only the DNA of AcMNPV or SfaMNPV can also induce apopotosis of SL-1 cell.Virus titer analysis revealed that neither viruses can duplicate in SL-1 cells.
文摘The crucial role of cell death in many diseases is obvious and has intense researches to understand the regulation of apoptotic pathways. Caspase activation is central to many of the apoptotic pathways. In recent years, the research on the regulation and activation of caspase has made a great progress. Caspase inhibitors prevent active caspases from committing the cell to irreversible destruction. This review will mainly focus on the characteristics of natural anticaspase inhibitor-IAP, regulation of IAP expression, mechanisms of action of IAP.
