Cross-resistance, resistance mechanisms, and mode of inheritance of spinosad resistance were studied in the western flower thrip, Frankliniella occidentalis （Pergande）. Spinosad （naturalyte insecticide） showed low...Cross-resistance, resistance mechanisms, and mode of inheritance of spinosad resistance were studied in the western flower thrip, Frankliniella occidentalis （Pergande）. Spinosad （naturalyte insecticide） showed low cross-resistance to prothiophos （organophosphorus insecticide） and chlorphenapyr （respiratory inhibitor） showed some cross-resistance to thiocyclam （nereistoxin）. The synergists PBO （piperonyl butoxide）, DEM （diethyl maleate）, and DEF （s,s,s-tributyl phosphorotrithioate） did not show any synergism on the toxicity of spinosad in the resistant strain （ICS）, indicating that metabolic- mediated detoxification was not responsible for the spinosad resistance, suggesting that spinosad may reduce sensitivity of the target site： the nicotinic acetylcholine receptor and GABA receptor. Following reciprocal crosses, dose-response lines and dominance ratios indicated that spinosad resistance was incompletely dominant and there were no maternal effects. The results of backcross showed that spinosad resistance did not fit a single-gene hypothesis, suggesting that resistance was influenced by several genes.展开更多
文摘Cross-resistance, resistance mechanisms, and mode of inheritance of spinosad resistance were studied in the western flower thrip, Frankliniella occidentalis （Pergande）. Spinosad （naturalyte insecticide） showed low cross-resistance to prothiophos （organophosphorus insecticide） and chlorphenapyr （respiratory inhibitor） showed some cross-resistance to thiocyclam （nereistoxin）. The synergists PBO （piperonyl butoxide）, DEM （diethyl maleate）, and DEF （s,s,s-tributyl phosphorotrithioate） did not show any synergism on the toxicity of spinosad in the resistant strain （ICS）, indicating that metabolic- mediated detoxification was not responsible for the spinosad resistance, suggesting that spinosad may reduce sensitivity of the target site： the nicotinic acetylcholine receptor and GABA receptor. Following reciprocal crosses, dose-response lines and dominance ratios indicated that spinosad resistance was incompletely dominant and there were no maternal effects. The results of backcross showed that spinosad resistance did not fit a single-gene hypothesis, suggesting that resistance was influenced by several genes.
