目的:探讨进一步提高免疫组织化学、原位杂交等显微图像定量分析的新方法。方法:从光密度和总面积两个因素综合考虑测试区域中阳性信号的强度,利用图像分析软件Image Pro Plus(IPP)分别将两者量化,综合使用阳性信号的平均光密度(MOD)和...目的:探讨进一步提高免疫组织化学、原位杂交等显微图像定量分析的新方法。方法:从光密度和总面积两个因素综合考虑测试区域中阳性信号的强度,利用图像分析软件Image Pro Plus(IPP)分别将两者量化,综合使用阳性信号的平均光密度(MOD)和面积比(total per area,TPA)两个参数进行表征。结果:经过对大量免疫组织化学、原位杂交等典型显微图像的测试,发现"MOD×TPA"能够更准确地反映待测区域内阳性信号的相对强度,尤其适用于用户指定的任意不规则测试区域图像的定量分析。结论:基于对图像分析原理进行综合分析后所得到的以上优化参数可实现显微图像的相对定量分析,为体视学分析方法提供了新的参考。展开更多
The forming-regions of phosphate glasses doped with high Sm2O3 contents were studied by two step melting method. The relationship between absorption spectrum and rare earth contents was also discussed. The spectra of ...The forming-regions of phosphate glasses doped with high Sm2O3 contents were studied by two step melting method. The relationship between absorption spectrum and rare earth contents was also discussed. The spectra of BASP glasses were measured by spectrophotometer. The results show that the forming regions will shrink with the increase of Al2O3 and it is difficult to form glass when [PO4]/[AlO4] < 2. The forming regions tend to be a closed elliptical, and the intensity of characteristic absorption peak increases gradually with the increase of Sm2O3, the absorption lines broadens non-uniformly and there is approximate linear relation between optical density at the 1064 nm wavelength and Sm2O3 content.展开更多
文摘目的:探讨进一步提高免疫组织化学、原位杂交等显微图像定量分析的新方法。方法:从光密度和总面积两个因素综合考虑测试区域中阳性信号的强度,利用图像分析软件Image Pro Plus(IPP)分别将两者量化,综合使用阳性信号的平均光密度(MOD)和面积比(total per area,TPA)两个参数进行表征。结果:经过对大量免疫组织化学、原位杂交等典型显微图像的测试,发现"MOD×TPA"能够更准确地反映待测区域内阳性信号的相对强度,尤其适用于用户指定的任意不规则测试区域图像的定量分析。结论:基于对图像分析原理进行综合分析后所得到的以上优化参数可实现显微图像的相对定量分析,为体视学分析方法提供了新的参考。
基金NSFC-Guangdong Province Joint Key Project (U1133003)Natural Science Foundation of China(41176104,31070103)Natural Science Foundation of Guangdong Province Key Project(10251007002000001)
基金Project supported by Research Project (A3820060136)
文摘The forming-regions of phosphate glasses doped with high Sm2O3 contents were studied by two step melting method. The relationship between absorption spectrum and rare earth contents was also discussed. The spectra of BASP glasses were measured by spectrophotometer. The results show that the forming regions will shrink with the increase of Al2O3 and it is difficult to form glass when [PO4]/[AlO4] < 2. The forming regions tend to be a closed elliptical, and the intensity of characteristic absorption peak increases gradually with the increase of Sm2O3, the absorption lines broadens non-uniformly and there is approximate linear relation between optical density at the 1064 nm wavelength and Sm2O3 content.
