背景:颞下颌关节骨关节炎是一种发病率很高的口腔常见病,而颞下颌关节骨关节炎早期隐匿不易被发现,临床上病理标本获取难度较高,难以开展相关研究。将数字化3D打印技术应用到颞下颌关节骨关节炎动物模型上来增加动物模型的一致性,从而...背景:颞下颌关节骨关节炎是一种发病率很高的口腔常见病,而颞下颌关节骨关节炎早期隐匿不易被发现,临床上病理标本获取难度较高,难以开展相关研究。将数字化3D打印技术应用到颞下颌关节骨关节炎动物模型上来增加动物模型的一致性,从而促进颞下颌关节骨关节炎疾病的研究。目的:利用新型数字技术建立标准化的颞下颌关节骨关节炎大鼠模型。方法:将30只SD雌性大鼠根据单侧前牙反牙合建模方法的不同,随机分为3组,传统模型组、数字化模型组和对照组,每组10只。于建模后4,8周采集髁突软骨标本,体式显微镜观察表观形态,苏木精-伊红染色、番红O/固绿染色观察病理形态,ELISA观察白细胞介素1β、肿瘤坏死因子α的表达变化,免疫组织化学染色观察蛋白多糖、Ⅱ型胶原及基质金属蛋白酶13的表达变化。结果与结论:(1)数字化模型组和传统模型组观察到不同程度的退行性病变。干预后第1周,模型组大鼠体质量均呈下降趋势,随后呈增长趋势,且显著低于对照组。(2)体式显微镜观察结果显示,建模后4,8周,数字化模型组表面的变形及缺损程度明显高于传统模型组。(3)两个时间点,数字化模型组和传统模型组的国际骨关节炎研究学会(Osteoarthritis Research Society International,OARSI)评分均高于对照组,数字化模型组高于传统模型组(P<0.05)。(4)组织病理学观察显示,建模后4,8周,两个模型组的改良Mankin评分和OARSI评分均显著高于同龄对照组(P<0.05)。(5)免疫组织化学染色结果显示,建模后4,8周,与同龄对照组相比,蛋白多糖及Ⅱ型胶原在传统模型组与数字化模型组两个时间点表达降低,基质金属蛋白酶13表达增强(P<0.05)。(6)ELISA结果显示,传统和数字化模型组大鼠8周时炎性因子白细胞介素1β和肿瘤坏死因子α的表达水平均高于同龄对照组,数字化模型组高于传统模型组(P<0.05)。(7)展开更多
AIM: To investigate whether congenital lumbar spinal stenosis(CLSS) is associated with a specific degenerative changes of the lumbar spine. METHODS: The lumbar spine magnetic resonance imaging studies of 52 subjects w...AIM: To investigate whether congenital lumbar spinal stenosis(CLSS) is associated with a specific degenerative changes of the lumbar spine. METHODS: The lumbar spine magnetic resonance imaging studies of 52 subjects with CLSS and 48 control subjects were retrospectively evaluated. In each examination, the five lumbar levels were assessed for the presence or absence of circumferential or shallow annular bulges, annular tears, anterior or posterior disc herniations, epidural lipomatosis, Schmorl's nodes,spondylolisthesis, pars defects, and stress reactions of the posterior vertebral elements. RESULTS: Compared to control individuals, subjects with CLSS exhibited increased incidence of circumferential and shallow annular bulges, annular tears, discherniations and spondylolisthesis(P < 0.05). CONCLUSION: CLSS is associated with increased incidence of degenerative changes in specific osseous and soft-tissue elements of the lumbar spine.展开更多
目的评价肩关节退行性改变程度与年龄的关系及其临床表现。方法200例患者共400肩,男48例(24％),女152例(76％),年龄70-101岁,平均为(84．2±6．7)岁。病史询问和理学检查的结果按Constant方法予以评分。所有患者均摄双肩旋转中立位...目的评价肩关节退行性改变程度与年龄的关系及其临床表现。方法200例患者共400肩,男48例(24％),女152例(76％),年龄70-101岁,平均为(84．2±6．7)岁。病史询问和理学检查的结果按Constant方法予以评分。所有患者均摄双肩旋转中立位、正位X线片,观察肩峰下间隙宽度(AHD)及盂肱关节的改变程度,并按Hamada方法进行分类。结果AHD≥6mm 349肩(87．25％),平均为(9．89±2．00)mm,对应HamadaⅠ型,AHD< 6 mm 51肩,平均为(3．08±1．70)mm,其中HamadaⅡ型21肩(5．25％),Ⅲ型16肩(4．00％),Ⅳ型5肩(1．25％),V型5肩(1．25％),另有4肩(1．00％)不能按Hamada分型进行归类。Hamada分型越高,Constant评分值越低。288肩(72．00％)盂舷关节间隙正常,93肩(23．25％)盂肱关节间隙正常但伴肱骨头或肩胛盂下方的骨赘形成,19肩(4．75％)盂肱关节狭窄或消失。随着盂肱关节间隙狭窄的程度加剧,Constant评分值降低,肩峰下间隙狭窄的发生率随之升高。在盂肱关节间隙改变的被调查者中,50％伴有肩峰下间隙的狭窄或消失。结论肩袖撕裂的发生率随年龄的增长而升高,但盂肱关节骨关节炎的发生率仅有轻微升高。单纯的肩峰下间隙狭窄或消失的临床症状较轻,而盂肱关节间隙狭窄伴或不伴AHD改变的临床症状剧烈,表现为肩关节疼痛和关节僵硬。展开更多
Objective: To find out, whether the degenerative process of ascending aorta is limited to the aortic sinuses only or is extending to mid and distal ascending aorta of Bicuspid Aortic Valve patients. Method: A prospect...Objective: To find out, whether the degenerative process of ascending aorta is limited to the aortic sinuses only or is extending to mid and distal ascending aorta of Bicuspid Aortic Valve patients. Method: A prospective consecutive study on 25 patients of BAV (undergoing aortic valve with ± ascending aortic surgeries) was conducted from 1st Jan 2010 to 30th Dec 2011. Morphological and anatomical data of root and ascending aorta were recorded from echocardiography and computed tomography angiography. Intra-operatively, aortic tissue biopsy taken from three sites: sinus, mid, and distal ascending aorta. Histological evaluation of the aortic wall was based on criteria adapted from Schlatmann and Becker and from de Sa et al. The presence and degree of the 5 variables of degeneration were studied: Linear regression and correlation were used to get relationship between histopathological scoring (HPE-T) and aortic diameter for each site of ascending aorta. Results: Significant linear relation was found between aortic sinus diameter and HPE T score with R value = 0.590 (p value 0.001) and variance of 37.5%. Analysis suggests that HPE T Score = -5.139 + (0.188 x Ao. Sinus Diameter in mm). No significant linear relation could be established between mid and distal ascending aorta diameter and HPE T scoring. Conclusion: In BAV patient’s, a definite relationship between degenerative changes of aortic media and aortic diameter was found and was limited up to the sinus level only. Thus, the study reinforces the thought of replacing aortic sinus too while dealing with aortic valve, even without significant dilatation. By this aggressive management of aortic root, progression of aortic dilatation or dissection can be prevented in bicuspid aortic valve patients.展开更多
文摘背景:颞下颌关节骨关节炎是一种发病率很高的口腔常见病,而颞下颌关节骨关节炎早期隐匿不易被发现,临床上病理标本获取难度较高,难以开展相关研究。将数字化3D打印技术应用到颞下颌关节骨关节炎动物模型上来增加动物模型的一致性,从而促进颞下颌关节骨关节炎疾病的研究。目的:利用新型数字技术建立标准化的颞下颌关节骨关节炎大鼠模型。方法:将30只SD雌性大鼠根据单侧前牙反牙合建模方法的不同,随机分为3组,传统模型组、数字化模型组和对照组,每组10只。于建模后4,8周采集髁突软骨标本,体式显微镜观察表观形态,苏木精-伊红染色、番红O/固绿染色观察病理形态,ELISA观察白细胞介素1β、肿瘤坏死因子α的表达变化,免疫组织化学染色观察蛋白多糖、Ⅱ型胶原及基质金属蛋白酶13的表达变化。结果与结论:(1)数字化模型组和传统模型组观察到不同程度的退行性病变。干预后第1周,模型组大鼠体质量均呈下降趋势,随后呈增长趋势,且显著低于对照组。(2)体式显微镜观察结果显示,建模后4,8周,数字化模型组表面的变形及缺损程度明显高于传统模型组。(3)两个时间点,数字化模型组和传统模型组的国际骨关节炎研究学会(Osteoarthritis Research Society International,OARSI)评分均高于对照组,数字化模型组高于传统模型组(P<0.05)。(4)组织病理学观察显示,建模后4,8周,两个模型组的改良Mankin评分和OARSI评分均显著高于同龄对照组(P<0.05)。(5)免疫组织化学染色结果显示,建模后4,8周,与同龄对照组相比,蛋白多糖及Ⅱ型胶原在传统模型组与数字化模型组两个时间点表达降低,基质金属蛋白酶13表达增强(P<0.05)。(6)ELISA结果显示,传统和数字化模型组大鼠8周时炎性因子白细胞介素1β和肿瘤坏死因子α的表达水平均高于同龄对照组,数字化模型组高于传统模型组(P<0.05)。(7)
文摘AIM: To investigate whether congenital lumbar spinal stenosis(CLSS) is associated with a specific degenerative changes of the lumbar spine. METHODS: The lumbar spine magnetic resonance imaging studies of 52 subjects with CLSS and 48 control subjects were retrospectively evaluated. In each examination, the five lumbar levels were assessed for the presence or absence of circumferential or shallow annular bulges, annular tears, anterior or posterior disc herniations, epidural lipomatosis, Schmorl's nodes,spondylolisthesis, pars defects, and stress reactions of the posterior vertebral elements. RESULTS: Compared to control individuals, subjects with CLSS exhibited increased incidence of circumferential and shallow annular bulges, annular tears, discherniations and spondylolisthesis(P < 0.05). CONCLUSION: CLSS is associated with increased incidence of degenerative changes in specific osseous and soft-tissue elements of the lumbar spine.
文摘目的评价肩关节退行性改变程度与年龄的关系及其临床表现。方法200例患者共400肩,男48例(24％),女152例(76％),年龄70-101岁,平均为(84．2±6．7)岁。病史询问和理学检查的结果按Constant方法予以评分。所有患者均摄双肩旋转中立位、正位X线片,观察肩峰下间隙宽度(AHD)及盂肱关节的改变程度,并按Hamada方法进行分类。结果AHD≥6mm 349肩(87．25％),平均为(9．89±2．00)mm,对应HamadaⅠ型,AHD< 6 mm 51肩,平均为(3．08±1．70)mm,其中HamadaⅡ型21肩(5．25％),Ⅲ型16肩(4．00％),Ⅳ型5肩(1．25％),V型5肩(1．25％),另有4肩(1．00％)不能按Hamada分型进行归类。Hamada分型越高,Constant评分值越低。288肩(72．00％)盂舷关节间隙正常,93肩(23．25％)盂肱关节间隙正常但伴肱骨头或肩胛盂下方的骨赘形成,19肩(4．75％)盂肱关节狭窄或消失。随着盂肱关节间隙狭窄的程度加剧,Constant评分值降低,肩峰下间隙狭窄的发生率随之升高。在盂肱关节间隙改变的被调查者中,50％伴有肩峰下间隙的狭窄或消失。结论肩袖撕裂的发生率随年龄的增长而升高,但盂肱关节骨关节炎的发生率仅有轻微升高。单纯的肩峰下间隙狭窄或消失的临床症状较轻,而盂肱关节间隙狭窄伴或不伴AHD改变的临床症状剧烈,表现为肩关节疼痛和关节僵硬。
文摘Objective: To find out, whether the degenerative process of ascending aorta is limited to the aortic sinuses only or is extending to mid and distal ascending aorta of Bicuspid Aortic Valve patients. Method: A prospective consecutive study on 25 patients of BAV (undergoing aortic valve with ± ascending aortic surgeries) was conducted from 1st Jan 2010 to 30th Dec 2011. Morphological and anatomical data of root and ascending aorta were recorded from echocardiography and computed tomography angiography. Intra-operatively, aortic tissue biopsy taken from three sites: sinus, mid, and distal ascending aorta. Histological evaluation of the aortic wall was based on criteria adapted from Schlatmann and Becker and from de Sa et al. The presence and degree of the 5 variables of degeneration were studied: Linear regression and correlation were used to get relationship between histopathological scoring (HPE-T) and aortic diameter for each site of ascending aorta. Results: Significant linear relation was found between aortic sinus diameter and HPE T score with R value = 0.590 (p value 0.001) and variance of 37.5%. Analysis suggests that HPE T Score = -5.139 + (0.188 x Ao. Sinus Diameter in mm). No significant linear relation could be established between mid and distal ascending aorta diameter and HPE T scoring. Conclusion: In BAV patient’s, a definite relationship between degenerative changes of aortic media and aortic diameter was found and was limited up to the sinus level only. Thus, the study reinforces the thought of replacing aortic sinus too while dealing with aortic valve, even without significant dilatation. By this aggressive management of aortic root, progression of aortic dilatation or dissection can be prevented in bicuspid aortic valve patients.
