To investigate (1) whether ischemic preconditioning (IPC) could protect immature rabbit hearts against ischemia reperfusion injury and (2) the role of K ATP channel in the mechanism of myocardial protection ...To investigate (1) whether ischemic preconditioning (IPC) could protect immature rabbit hearts against ischemia reperfusion injury and (2) the role of K ATP channel in the mechanism of myocardial protection Since cardioplegia is a t raditional and effective cardioprotective measure in clinic, our study is also d esigned to probe the compatibility between IPC and cardioplegia Methods New Zealand rabbits aged 14-21 days weighing 220-280 g were used The animals w ere anesthetized and heparinized The chest was opened and the heart was quickl y removed for connection of the aorta via Langendorff's method within 30 s after excision All hearts were perfused with Krebs Henseleit buffer balanced with gas mixture (O 2∶CO 2=95%∶5%) at 60 cm H 2O (perfusion pressure) IPC cons isted of 5 min global ischemia plus 10 min reperfusion Glibenclamide was used as the K ATP channel blocker at a concentration of 10 μmol/L before IPC Cardiac arrest was induced with 4℃ St Thomas cardioplegic solution, at which point the heart was made globally ischemic by withholding perfusion for 45 min f ollowed by 40 min reperfusion Thirty immature rabbit hearts were randomly divi ded into four groups: CON (n=9) was subjected to ischemia reperfusion only; IPC ( n=9) underwent IPC and ischemia reperfusion; Gli (n=6) was given glibenclamide and ischemia reperfusion; and Gli+IPC (n=6) underwent glibenclamide, IPC and is chemia reperfusion Coronary flow (CF), HR, left ventricle developed pressure (LVDP), and ±dp/dt max were monitored at equilibration (baseline value) an d 5, 1 0, 20, 30 and 40 min after reperfusion The values resulting from reperfusion w ere expressed as a percentage of their baseline values Arrhythmia quantificati on, myocardial enzyme in the coronary effluent and myocardial energy metaboli sm were also determined Results The recovery of CF, HR, LVDP and ±dp/dt max in preconditioned hearts was b est am ong the four groups The incidence of arrhythmia was low a展开更多
Background: Blood cardioplegia is a technique with many variations in its use. Intermittent cross clamping with Lidoflazine has proven to deliver good cardioprotection in our center. Question: Is tepid (32°C) ant...Background: Blood cardioplegia is a technique with many variations in its use. Intermittent cross clamping with Lidoflazine has proven to deliver good cardioprotection in our center. Question: Is tepid (32°C) antegrade intermittent blood cardioplegia an efficient, safe and easy-to-use alternative to intermittent cross-clamping with Lidoflazine in elective isolated CABG in low-risk patients? Primary outcomes are heart enzymes (cTnI, CK-MB). Secondary outcomes are operation times, length of hospital stay, major complications and in-hospital mortality. Methods: From January 2012 until November 2012, 40 patients with LVEF ≥50%, EuroSCORE展开更多
文摘To investigate (1) whether ischemic preconditioning (IPC) could protect immature rabbit hearts against ischemia reperfusion injury and (2) the role of K ATP channel in the mechanism of myocardial protection Since cardioplegia is a t raditional and effective cardioprotective measure in clinic, our study is also d esigned to probe the compatibility between IPC and cardioplegia Methods New Zealand rabbits aged 14-21 days weighing 220-280 g were used The animals w ere anesthetized and heparinized The chest was opened and the heart was quickl y removed for connection of the aorta via Langendorff's method within 30 s after excision All hearts were perfused with Krebs Henseleit buffer balanced with gas mixture (O 2∶CO 2=95%∶5%) at 60 cm H 2O (perfusion pressure) IPC cons isted of 5 min global ischemia plus 10 min reperfusion Glibenclamide was used as the K ATP channel blocker at a concentration of 10 μmol/L before IPC Cardiac arrest was induced with 4℃ St Thomas cardioplegic solution, at which point the heart was made globally ischemic by withholding perfusion for 45 min f ollowed by 40 min reperfusion Thirty immature rabbit hearts were randomly divi ded into four groups: CON (n=9) was subjected to ischemia reperfusion only; IPC ( n=9) underwent IPC and ischemia reperfusion; Gli (n=6) was given glibenclamide and ischemia reperfusion; and Gli+IPC (n=6) underwent glibenclamide, IPC and is chemia reperfusion Coronary flow (CF), HR, left ventricle developed pressure (LVDP), and ±dp/dt max were monitored at equilibration (baseline value) an d 5, 1 0, 20, 30 and 40 min after reperfusion The values resulting from reperfusion w ere expressed as a percentage of their baseline values Arrhythmia quantificati on, myocardial enzyme in the coronary effluent and myocardial energy metaboli sm were also determined Results The recovery of CF, HR, LVDP and ±dp/dt max in preconditioned hearts was b est am ong the four groups The incidence of arrhythmia was low a
文摘Background: Blood cardioplegia is a technique with many variations in its use. Intermittent cross clamping with Lidoflazine has proven to deliver good cardioprotection in our center. Question: Is tepid (32°C) antegrade intermittent blood cardioplegia an efficient, safe and easy-to-use alternative to intermittent cross-clamping with Lidoflazine in elective isolated CABG in low-risk patients? Primary outcomes are heart enzymes (cTnI, CK-MB). Secondary outcomes are operation times, length of hospital stay, major complications and in-hospital mortality. Methods: From January 2012 until November 2012, 40 patients with LVEF ≥50%, EuroSCORE
