Urinary prothrombin fragment 1 （UPTFl） is a potent inhibitor of urinary stone formation. UPTF1 exerts such inhibitory effect by effective 7-carboxylation in which vitamin K epoxide reductase complex subunit 1 （VKO...Urinary prothrombin fragment 1 （UPTFl） is a potent inhibitor of urinary stone formation. UPTF1 exerts such inhibitory effect by effective 7-carboxylation in which vitamin K epoxide reductase complex subunit 1 （VKORC1）, the rate-limiting enzyme, is involved. This study examined the correlation between VKORC1 expression and calcium oxalate urolithiasis. The renal cortex samples were obtained from patients undergoing nephrectomy and then divided into 3 groups： urolithiasis group, control group A [hydronephrosis-without-stone （HWS） group], control group B （normal control group）, The localization and expression of VKORC1 in renal tissues were determined by using immunohistochemistry, immunofluorescence microscopy, Western blotting and SYBR Green I real-time reverse-transcription PCR. The rapid amplification of cDNA ends （RACE） were conducted to obtain the 3＇- and 5＇-untranslated region （UTR） of VKORC1. The results showed that VKORC1 was located in the cytoplasm of renal tubular epithelial cells. The expression of VKORC1 in the uro- lithiasis group was significantly lower than that in the other two control groups （P〈0.05）. Moreover, the 3＇- and 5＇-UTR sequence of the VKORC1 gene was successfully cloned. No insertion or deletion was found in the 3＇- and 5＇-UTR. However, a 171-bp new base sequence was discovered in the up- stream of 5＇-UTR end in the urolithiasis group. It was concluded that the decreased expression of VKORC 1 may contribute to the development of calcium oxalate urolithiasis in the kidney.展开更多
The paper deals with the g2-stability analysis of multi-input-multi-output （MIMO） systems, governed by integral equations, with a matrix of periodic/aperiodic time-varying gains and a vector of monotone, non-monoton...The paper deals with the g2-stability analysis of multi-input-multi-output （MIMO） systems, governed by integral equations, with a matrix of periodic/aperiodic time-varying gains and a vector of monotone, non-monotone and quasi-monotone nonlin- earities. For nonlinear MIMO systems that are described by differential equations, most of the literature on stability is based on an application of quadratic forms as Lyapunov-function candidates. In contrast, a non-Lyapunov framework is employed here to derive new and more general g2-stability conditions in the frequency domain. These conditions have the following features： i） They are expressed in terms of the positive definiteness of the real part of matrices involving the transfer function of the linear time-invariant block and a matrix multiplier function that incorporates the minimax properties of the time-varying linear/nonlinear block, ii） For certain cases of the periodic time-varying gain, they contain, depending on the multiplier function chosen, no restrictions on the normalized rate of variation of the time-varying gain, but, for other periodic/aperiodic time-varying gains, they do. Overall, even when specialized to periodic-coefficient linear and nonlinear MIMO systems, the stability conditions are distinct from and less restrictive than recent results in the literature. No comparable results exist in the literature for aperiodic time-varying gains. Furthermore, some new stability results concerning the dwell-time problem and time-varying gain switching in linear and nonlinear MIMO systems with periodic/aperiodic matrix gains are also presented. Examples are given to illustrate a few of the stability theorems.展开更多
基金supported by a grant from the National Natural Science Foundation of China(No.30901482)
文摘Urinary prothrombin fragment 1 （UPTFl） is a potent inhibitor of urinary stone formation. UPTF1 exerts such inhibitory effect by effective 7-carboxylation in which vitamin K epoxide reductase complex subunit 1 （VKORC1）, the rate-limiting enzyme, is involved. This study examined the correlation between VKORC1 expression and calcium oxalate urolithiasis. The renal cortex samples were obtained from patients undergoing nephrectomy and then divided into 3 groups： urolithiasis group, control group A [hydronephrosis-without-stone （HWS） group], control group B （normal control group）, The localization and expression of VKORC1 in renal tissues were determined by using immunohistochemistry, immunofluorescence microscopy, Western blotting and SYBR Green I real-time reverse-transcription PCR. The rapid amplification of cDNA ends （RACE） were conducted to obtain the 3＇- and 5＇-untranslated region （UTR） of VKORC1. The results showed that VKORC1 was located in the cytoplasm of renal tubular epithelial cells. The expression of VKORC1 in the uro- lithiasis group was significantly lower than that in the other two control groups （P〈0.05）. Moreover, the 3＇- and 5＇-UTR sequence of the VKORC1 gene was successfully cloned. No insertion or deletion was found in the 3＇- and 5＇-UTR. However, a 171-bp new base sequence was discovered in the up- stream of 5＇-UTR end in the urolithiasis group. It was concluded that the decreased expression of VKORC 1 may contribute to the development of calcium oxalate urolithiasis in the kidney.
文摘The paper deals with the g2-stability analysis of multi-input-multi-output （MIMO） systems, governed by integral equations, with a matrix of periodic/aperiodic time-varying gains and a vector of monotone, non-monotone and quasi-monotone nonlin- earities. For nonlinear MIMO systems that are described by differential equations, most of the literature on stability is based on an application of quadratic forms as Lyapunov-function candidates. In contrast, a non-Lyapunov framework is employed here to derive new and more general g2-stability conditions in the frequency domain. These conditions have the following features： i） They are expressed in terms of the positive definiteness of the real part of matrices involving the transfer function of the linear time-invariant block and a matrix multiplier function that incorporates the minimax properties of the time-varying linear/nonlinear block, ii） For certain cases of the periodic time-varying gain, they contain, depending on the multiplier function chosen, no restrictions on the normalized rate of variation of the time-varying gain, but, for other periodic/aperiodic time-varying gains, they do. Overall, even when specialized to periodic-coefficient linear and nonlinear MIMO systems, the stability conditions are distinct from and less restrictive than recent results in the literature. No comparable results exist in the literature for aperiodic time-varying gains. Furthermore, some new stability results concerning the dwell-time problem and time-varying gain switching in linear and nonlinear MIMO systems with periodic/aperiodic matrix gains are also presented. Examples are given to illustrate a few of the stability theorems.
