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低温胁迫下长豇豆幼苗可溶性蛋白质和细胞保护酶活性的变化 被引量:42
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作者 陈禅友 汪汇东 丁毅 《园艺学报》 CAS CSCD 北大核心 2005年第5期911-913,共3页
对12个长豇豆品种幼苗进行10℃低温胁迫处理,室温(20±3)℃作对照,5 d后测定叶片可溶性蛋白质含量和4种细胞保护酶活性.结果表明:低温胁迫下,6个早熟品种可溶性蛋白质含量较相应对照显著升高2.3%~8.1%, 3个晚熟品种显著降低;8个品... 对12个长豇豆品种幼苗进行10℃低温胁迫处理,室温(20±3)℃作对照,5 d后测定叶片可溶性蛋白质含量和4种细胞保护酶活性.结果表明:低温胁迫下,6个早熟品种可溶性蛋白质含量较相应对照显著升高2.3%~8.1%, 3个晚熟品种显著降低;8个品种SOD活性显著升高27.82%~128.52%;4个早熟品种APX活性显著升高10.53%~39.36%,3个中晚熟品种略有降低.这3项指标能反映豇豆品种耐寒性,耐寒性品种3项指标升高,以SOD活性变化最剧烈.CAT和POD活性变化不适合作为长豇豆耐寒性鉴定指标. 展开更多
关键词 豇豆 长豇豆 低温胁迫 可溶性蛋白质 细胞保护酶活性
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豇豆属3种主要食用豆类的抗豆象育种研究进展 被引量:20
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作者 刘长友 田静 +4 位作者 范保杰 曹志敏 苏秋竹 张志肖 王素华 《中国农业科学》 CAS CSCD 北大核心 2010年第12期2410-2417,共8页
在中国栽培的豇豆属食用豆类主要有绿豆(Vigna radiata)、小豆(Vigna angularis)和豇豆(Vigna unguiculata)。豆象是危害这些食用豆类作物的重要仓储害虫。防治豆象危害最为经济且环保的方法是利用作物本身的抗性培育抗豆象品种。中国... 在中国栽培的豇豆属食用豆类主要有绿豆(Vigna radiata)、小豆(Vigna angularis)和豇豆(Vigna unguiculata)。豆象是危害这些食用豆类作物的重要仓储害虫。防治豆象危害最为经济且环保的方法是利用作物本身的抗性培育抗豆象品种。中国豇豆属食用豆类抗豆象育种尚处于起步阶段,分子遗传学研究落后,导致抗豆象新品种选育进程缓慢、育种效率低。本文将从抗豆象资源搜集、抗豆象机理研究以及抗豆象分子标记、抗豆象育种等方面综合阐述绿豆、小豆和豇豆3种主要豇豆属食用豆类的国内外研究进展,以期对中国开展相关的研究提供借鉴。 展开更多
关键词 豇豆属 豆象 研究进展
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基于SSR标记分析小豆及其近缘植物的遗传关系 被引量:12
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作者 王丽侠 程须珍 王素华 《生物多样性》 CAS CSCD 北大核心 2011年第1期17-23,共7页
本研究利用87对SSR引物分析了80份栽培小豆(Vigna angularis)、22份野生小豆(V.angularis var. nipponensis)以及10份豇豆属(共7个种)近缘植物,旨在比较豇豆属不同种的遗传多样性,并分析种间的遗传关系。结果显示87对SSR引物在112份小... 本研究利用87对SSR引物分析了80份栽培小豆(Vigna angularis)、22份野生小豆(V.angularis var. nipponensis)以及10份豇豆属(共7个种)近缘植物,旨在比较豇豆属不同种的遗传多样性,并分析种间的遗传关系。结果显示87对SSR引物在112份小豆及其近缘植物资源中共检测到667个等位变异,其中有75个、71个和82个SSR位点分别在栽培小豆、野生小豆和近缘植物中表现为多态。随机抽样分析发现,平均每SSR位点检测到的等位变异数目为近缘植物>野生小豆>栽培小豆,与多态信息含量(PIC)值一致,说明近缘植物及野生小豆中蕴含着丰富的遗传变异,是栽培小豆育种的重要基因来源。聚类分析显示,栽培小豆、野生小豆和近缘植物间的遗传分化比较明显,分别聚为三大类,其中栽培小豆的遗传背景与其生态环境相对应;近缘植物又可以分为三个亚类,亚类间的遗传距离与其亲缘关系相对应。本研究结果也说明利用SSR标记辅助豇豆属的种间分类是可行的。 展开更多
关键词 豇豆属植物 SSR 遗传多样性 遗传关系
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Construction of an integrated map and location of a bruchid resistance gene in mung bean 被引量:9
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作者 Lixia Wang Chuanshu Wu +6 位作者 Min Zhong Dan Zhao Li Mei Honglin Chen Suhua Wang Chunji Liu Xuzhen Cheng 《The Crop Journal》 SCIE CAS CSCD 2016年第5期360-366,共7页
Bruchid beetle(Callosobruchus chinensis) poses a serious threat to the production and storage of mung bean(Vigna radiata). Mapping bruchid resistance(Br) will provide an important basis for cloning the responsible gen... Bruchid beetle(Callosobruchus chinensis) poses a serious threat to the production and storage of mung bean(Vigna radiata). Mapping bruchid resistance(Br) will provide an important basis for cloning the responsible gene(s) and elucidating its functional mechanism, and will also facilitate marker-assisted selection in mung bean breeding. Here, we report the construction of the genetic linkage groups of mung bean and mapping of the Br1 locus using an RIL population derived from a cross between Berken, a bruchid-susceptible line, and ACC41, a bruchid-resistant line. A total of 560 markers were mapped onto 11 linkage groups,with 38.0% of the markers showing distorted segregation. The lengths of the linkage groups ranged from 45.2 to 117.0 c M with a total coverage of 732.9 c M and an average interval of1.3 c M between loci. Br1 was located on LG9 between BM202(0.7 c M) and Vr2-627(1.7 c M).Based on 270 shared SSR markers, most of the linkage groups were assigned to specific chromosomes. These results should further accelerate the genetic study of this crop. 展开更多
关键词 vigna radiata Callosobruchus chinensis Linkage map RESISTANCE Gene mapping
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Genetic Diversity in Cowpea (Vigna unguiculata (L.) Walp) under Two Growing Conditions*
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作者 Omotola Oluwakemi Dairo 《Advances in Bioscience and Biotechnology》 CAS 2024年第5期310-324,共15页
This study explores the use of genetic variability for advancing the genetic improvement of Cowpea (Vigna unguiculata (L.) Walp), particularly in response to insect infestation stress. Over a period spanning 2015 to 2... This study explores the use of genetic variability for advancing the genetic improvement of Cowpea (Vigna unguiculata (L.) Walp), particularly in response to insect infestation stress. Over a period spanning 2015 to 2017, forty accessions of cowpeas were evaluated to determine their variability under both insecticide spray and no insecticide spray conditions at the Teachings and Research Farms, Federal University of Agriculture, Abeokuta. The experimental design was a randomized complete block design in three replicates. The accessions were evaluated for plant height, leaf length, leaf width, number of days of 50% flowering, number of pods per plant, pod length, number of seeds per plant, 100-seed weight, and seed yield. Data collected were subjected to principal component and single linkage cluster analyses. Principal axis I (PCA1) accounted for 39% and 35% under insecticide spray and no insecticide spray respectively to the total variation in the accessions. Plant height with a factor score of 0.38, leaf length (0.41), number of leaves (0.37), and 100-seed, weight (0.30) was related to PCAI under insecticide spray while leaf width (0.32). Pod length (0.37) and number of seeds/plant (0.38) were significant to PCA1 under no insecticide spray. Notably, accessions such as SAMPEA6, SAMPEA10, IFE-Brown, and IFE-BPE exhibited consistent performance across both conditions, while others displayed condition-specific attributes. For instance, NGB1063, NGB1152, and NGB1093 demonstrated distinct traits under insecticide spray, while NGB1146 and NGB1124 exhibited notable characteristics under no insecticide spray conditions. Therefore, identifying these forty accessions with desirable traits hold promise for future genetic improvement efforts of cowpea cultivation in Nigeria and beyond. 展开更多
关键词 COWPEA vigna Unguiculata Genetic Diversity Cowpea Accessions
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Effect of Salt Stress on Photosynthetic GasExchange and Chlorophyll FluorescenceParameters in Alhagi pseudalhagi 被引量:6
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作者 Mahmut SHAWKAT Mansur NASIR +1 位作者 Qijun CHEN Halil KURBAN 《Agricultural Science & Technology》 CAS 2017年第3期411-416,423,共7页
Soil salinity is one of the most important environmental factors limiting plant growth and productivity in many regions in the world. Salt stress directly affects plant photosynthesis, which is an important physiologi... Soil salinity is one of the most important environmental factors limiting plant growth and productivity in many regions in the world. Salt stress directly affects plant photosynthesis, which is an important physiological process to limit plant growth and crop yield. But the effects of salt stress on the mechanism of the photosynthesis is still not clear. In this experiment, the salt tolerant plant Alhagi pseudalhagi was selected as the experiment material, and the salt sensitive plant Vigna radiata as the control, to explore the effects of salt stress on photosynthetic parameters of A. pseudalhagi. Plants were grown in a greenhouse,cultured with 1/2 Hoagland nutrient solution, treated by 0(control), 50, 100 or 200 s of mmol/L of NaCl solution for 12 d. Then,the chlorophyll contents, gas exchange parameters and chlorophyll a fluorescence in each treatment were measured. The results showed that under the salt stress simulated by 50 mmol/L NaCl, the net photosynthetic rate(Pn) and stomatal conductance(gs)of A. pseudalhagi were significantly increased compared with the control, while intercellular CO_2 concentration(Ci) was significantly decreased, Fv'/Fm', Fv/Fm and Fv/Fo were increased over time, but had no significant differences with the control ФPSⅡ,ETR and qP were significantly increased, and NPQ was significantly decreased. Under the salt stress simulated by 100 and 200mmol/L NaCl, Fv'/Fm', Fv/Fm, Fv/Fo, ФPSⅡ and qP of A. pseudalhagi were gradually decreased over time. In contrary, under the salt stress simulated by 50 and 100 mmol/L NaCl, the Pn, gs, Ci, Fv'/Fm', Fv/Fm, Fv/Fo, ФPSII, qP and ETR were all significantly decreased, while NPQ was significantly increased. 展开更多
关键词 SALINITY STRESS PHOTOSYNTHETIC PARAMETER Alhagi pseudalhagi vigna RADIATA
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Phytochemical Composition and Insecticidal Property of Native Plants against the Cowpea Weevil Callosobruchus maculatus Fab. (Coleoptera: Bruchidae) for Natural Preservation of Cowpea Seeds
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作者 Elias Mano Rabièta Simde +6 位作者 Koï Wenceslas Kam Nadjatou Ouédraogo Désiré Dramane Abdoul Khader Sanou Rasmané Abdou Ouédraogo Boubakar Ouattara Pierre Alexandre Eric Djifaby Sombié Souleymane Nacro 《Advances in Entomology》 2024年第1期56-66,共11页
About 80% of cowpea seeds are lost along with 30% of their weight after six months of unprotected storage due to attacks by Callosobruchus maculatus. This study aimed at evaluating the effect of applying bioinsecticid... About 80% of cowpea seeds are lost along with 30% of their weight after six months of unprotected storage due to attacks by Callosobruchus maculatus. This study aimed at evaluating the effect of applying bioinsecticides on the biological parameters of C. maculatus to reduce its damage to cowpea stocks. Four treatments including an untreated control and three doses of powdery extracts of C. nigricans, Z. zanthoxyloides and H. suaveolens, were compared in a Fischer block with four completely randomized repetitions. One hundred undamaged cowpea seeds were mixed with doses of each extract in Petri dishes and then fed as food substrates to ten pairs of C. maculatus for eight days. The extracts of H. suaveolens, C. nigricans and Z. zanthoxyloides all contain active ingredients including polyphenols, flavonoids, anthocyanins and alkaloids, etc. The extract of Z. zanthoxyloides was found to be more active on adults (100.00% mortality) followed by C. nigricans (98.75% mortality) and H. suaveolens (97.50% mortality) at 80 mg/g. Fecundity per female decreased significantly in the treated batches of 13.82 eggs for H. suaveolens at 10.12 eggs for C. nigricans at a dose of 80 mg/g. Germination rate of seeds was statistically raised to 100% at 60 and 80 mg/g by C. nigricans powder and lowered to 83.00% at 20 mg/g by Z. zanthoxyloides extracts. The application of Z. zanthoxyloides extract would be a less expensive alternative for the sustainable management of C. maculatus in cowpea stocks. 展开更多
关键词 Cassia nigricans Hyptis suaveolens Zanthoxylum zanthoxyloides Biological Activities vigna unguiculata Pest Burkina Faso
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Studies on the management of root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex of green gram, Vigna radiata cv ML-1108 被引量:7
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作者 HASEEB Akhtar SHARMA Anita SHUKLA Prabhat Kumar 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第8期736-742,共7页
Studies were conducted under pot conditions to determine the comparative efficacy ofcarbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould... Studies were conducted under pot conditions to determine the comparative efficacy ofcarbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode, Meloidogyne incognita-wilt fungus, Fusarium oxysporum disease complex on green gram, Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared carbofuran and A. indica seed powder increased plant growth to untreated inoculated plants. Analysis of data showed that and yield significantly more in comparison to bavistin and P. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus, A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens. 展开更多
关键词 Meloidogyne incognita Fusarium oxysporum Disease complex MANAGEMENT vigna radiata
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The Sequence Variations of Intron-3 of the α-Amylase Gene in Adzuki Bean 被引量:2
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作者 JINWen-lin YamaguchiHirofumi +1 位作者 IsigamiMatiko YasudaKentaro 《Agricultural Sciences in China》 CAS CSCD 2003年第10期1076-1082,共7页
This study describes variation of intron-3 of α-amylase gene from 156 breeds of adzuki beans using SSCP(single-strand conformation polymorphism)analysis. Based on α-amylase gene structure and sequence, A pair of P... This study describes variation of intron-3 of α-amylase gene from 156 breeds of adzuki beans using SSCP(single-strand conformation polymorphism)analysis. Based on α-amylase gene structure and sequence, A pair of PCR primers, F (CCTACATTCTAACACACCCT) and R (GCATATTGTGCCAGTACAAT) were designed to amplify intron-3 fragments of α-amylase gene. 14 variant types were detected, including 13, 9, 10, 4 variant types in the wild, weed, locally cultivated and modern brought-up adzuki beans respectively, 9, 8, 7 variant types of the wild adzuki beans from Japan, China and Korea respectively, and some other variant types in the local adzuki beans from China and Bhutan. 60% of subjects of cultivated races were found to be EE type in the experiment. In addition, sequence analysis of intron-3 of α-amylase gene from 8 variant types reveals the evolution process of various variant types in adzuki beans. 展开更多
关键词 Adzuki bean (vigna angularis) SSCP (single-strand conformation polymorphism) α- Amylase gene INTRON VARIATION
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Plants Extracts and a Mycoinsecticide in Cowpea Yield Improvement in Guinean Savanah and Sahelian Savanah Agro-Ecological Zones of Cameroun
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作者 Raoul Borkeum Barry Katamssadan Haman Tofel +4 位作者 Jean Wini Goudoungou François Ndosinvian Vandi Manuele Tamò Albert Ngakou Elias Nchiwan Nukenine 《Agricultural Sciences》 2023年第8期1038-1052,共15页
Cowpea yield improvement is done by adding agricultural inputs. The use of natural substances as pesticides is being encouraged to fight against cowpea field pests. The pesticidal potentials of Azadirachta indica and ... Cowpea yield improvement is done by adding agricultural inputs. The use of natural substances as pesticides is being encouraged to fight against cowpea field pests. The pesticidal potentials of Azadirachta indica and Boswellia dalzielii water extracts, Metarhizium anisopliae, alone and in combination with plant extracts, and a commercial synthetic pesticide (Decis<sup>®</sup>) were tested in field on two varieties of cowpeas, Vigna unguiculata in two agroecological zones (Guinean Savanah and Sahelian Savanah) of Cameroon. The field trials were carried out in a full randomized block design including nine treatments and a control. Four replications were made concerning the different treatments and control. At the flowering stage, the cowpea field was sprayed three times with different pesticidal formulations at the interval of five days. The number of ramifications per plant, and that of pods per block and seed yield were determined. The pesticide formulations considerably (p ®</sup>). Bafia variety treated with the combination of M. anisopliae and A. indica recorded the highest ramification rate (37.03 ± 1.59) in Maroua (Sahelian Savanah). The same variety also produced more important pods number (90.50 ± 16.66) in Ngaoundere (Guinean Savanah) with the binary combination of two plants used in this experiment. The highest seed yield (44.23 ± 2.31) was recorded in Ngaoundere with B125 variety treated with the combination of the three pesticidal formulations separately (A. indica, B. dalzielii, M. anisopliae). The plant products used in this work, M. anisopliae and their mixtures could supersede the synthetic pesticides considering environmental issue in cowpea crop protection. Then, such formulations would not only improve crop productivity but also preserve environment from the pollution due to the use of synthetic residual chemicals. 展开更多
关键词 Azadirachta indica Boswellia dalzielii Metarhizium anisopliae vigna unguiculata VARIETIES YIELD
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芦丁对绿豆幼苗营养生长的影响及其与IAA的相互作用(英文) 被引量:4
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作者 梁红 Yoneo Sagawa Qing Xiao LI 《植物生理与分子生物学学报》 CAS CSCD 北大核心 2005年第4期361-368,共8页
观察了植物体内的天然黄酮芦丁和吲哚乙酸(IAA)对绿豆幼苗营养生长的影响并测定胚轴中的芦丁和IAA含量.光照条件下芦丁(60μg/mL以下)处理对绿豆幼苗生长有一定促进作用,表现为胚轴和主根伸长加快、侧根数目增多、鲜重或干重增加;而光... 观察了植物体内的天然黄酮芦丁和吲哚乙酸(IAA)对绿豆幼苗营养生长的影响并测定胚轴中的芦丁和IAA含量.光照条件下芦丁(60μg/mL以下)处理对绿豆幼苗生长有一定促进作用,表现为胚轴和主根伸长加快、侧根数目增多、鲜重或干重增加;而光照条件下更高浓度芦丁(80μg/mL以上)处理及黑暗条件下芦丁(20~100μg/mL)处理对绿豆幼苗生长有抑制作用.当培养基中的芦丁浓度为60~80 μg/mL时,光照下的幼苗比暗处理的幼苗在胚轴中积累更多的芦丁;而芦丁浓度为40μg/mL以下和接近100μg/mL时幼苗在光照下累积的芦丁较暗处理的幼苗更少.0.1μg/mL以上的IAA促进芦丁的累积而进一步抑制幼苗胚轴和主根的伸长.当培养基中含有40 μg/mL的芦丁和0.5μg/mL的IAA时,胚轴中累积的芦丁达到高峰.芦丁降低黄化幼苗内源性IAA在胚轴中的累积,并抑制幼苗对IAA的吸收. 展开更多
关键词 生长 IAA 绿豆 芦丁 vigna radintn
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Screen House Assessment of Cowpea [(Vigna unguiculata (L.)] Genotypes for Drought Tolerance Using Selection Indices
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作者 Sekou Armand Sanogo Sory Diallo +3 位作者 Teyioue Benoit Joseph Batieno Adejumobi Idris Ishola Nerbéwendé Sawadogo Daniel Nyadanu 《Agricultural Sciences》 CAS 2023年第4期457-473,共17页
Cowpea [Vigna unguiculata (L.)] is one of the most important arid legumes cultivated for pulse and forage production. Drought is one of the most damageable constraints to crop production impacting negatively food secu... Cowpea [Vigna unguiculata (L.)] is one of the most important arid legumes cultivated for pulse and forage production. Drought is one of the most damageable constraints to crop production impacting negatively food security. The potential of cowpea to address food security is well established. However, not much is known about the base index selection method in breeding cowpea for drought tolerance, which is important for yields. Consequently, the present study has been conducted to: 1) evaluate the yield performance of cowpea genotypes under artificial drought and well-watered condition, 2) ranke genotype performance using selection indices, and 3) assess relationship between agronomic traits and yield. The experiment was the 2 watering conditions laid out in a Randomized Complete Block Design (RCBD) with three replications. The experiment was carried out in pots under screen house at the Department of Horticulture at KNUST. The result showed that KPR1-96-73, Simbo, CZ06-4-16, Wilibaly and Agyenkwa were high yielding in well-water condition while Ghana Shoba, Sangaraka, Nketewade, Ghana Shoni and Korobalen were high yielding genotypes in water stress condition. The average yield reduction was 60.6% and 16% for grain and fodder yield respectively. The biplot displays revealed four groups among the genotypes tested which were based on their yielding capacity and drought tolerance. In cluster B high yielding and drought tolerant genotypes were identified, high yielding and drought susceptible have been identified in cluster A, low yielding and drought tolerant in cluster D, and lastly low yielding and drought susceptible in cluster C. Genotypes in cluster B were best due to the fact that it combines high yield and tolerance to drought. They were Ghana Shoni, Nketewade, Sangaraka and Ghana Shoba. These genotypes might be suitably employed in further drought tolerance breeding programs of cowpea. Significant relationships were observed between agronomic trait and yields under drought condition. 展开更多
关键词 Cowpea [vigna unguiculata (L.)] DROUGHT Selection Index
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Agro-Morphological Variability in Eight Bambara Groundnut (Vigna subterranea (L.) Verdc.) Morphotypes from Zinder (Niger)
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作者 Oumarou Zango Rabiou Abdou +2 位作者 Saley Adamou Salamatou Agbo So Timothée Kouassi Bakasso Yacoubou 《Agricultural Sciences》 CAS 2023年第1期1-10,共10页
Bambara groundnut (Vigna subterranea (L.) Verdc.) has economic importance for the producers and the traders as cash crop since it generates substantial income for households in Niger. The objective of this study was t... Bambara groundnut (Vigna subterranea (L.) Verdc.) has economic importance for the producers and the traders as cash crop since it generates substantial income for households in Niger. The objective of this study was to assess eight (8) Bambara groundnut morphotype from Université André Salifou de Zinder in Niger Republic by their morphological and agronomic characters. The experiment was conducted in a randomized complete block design with three replications during the rainy season of 2020-2021. Data were collected on the Number of leaves, Plant height (cm), Growth habit (cm), Terminal leaflet length, Terminal leaflet width, Number of nodes/stem, Number of stems/plant, Number of pods/plant, Weight of 100 pods (g), Weight of 100 seeds (g), Length of seeds (mm), Width of seeds (mm), Weight of seeds/Plot (kg). There were significant differences for all characters, except Number of leaves, Terminal leaflet length, Terminal leaflet width, Length of seeds (mm) Weight of 100 pods (g), Weight of 100 seeds (g). The morphotypes UZ-VZ-04, UZ-VZ-03, UZ-VZ-06, UZ-VZ-02 and UZ-VZ-05 have demonstrated good performance for grain weight per plot and can be useful for a breeding program. 展开更多
关键词 vigna subterranea ACCESSIONS Agro-Morphological Traits Weight of 100 Seeds
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Selection of Cowpea [(Vigna unguiculata (L.) WALP] Genotypes for Drought Tolerance Using Selection Indices
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作者 Sekou Armand Sanogo Sory Diallo +2 位作者 Daniel Nyadanu Téyioué Benoit Joseph Batieno Nerbéwendé Sawadogo 《Agricultural Sciences》 CAS 2023年第3期384-397,共14页
Cowpea [(Vigna unguiculata (L.)] is one of the most important arid legumes cultivated for pulse and forage production. However, in cowpea, not much is known about the base index selection method in breeding for drough... Cowpea [(Vigna unguiculata (L.)] is one of the most important arid legumes cultivated for pulse and forage production. However, in cowpea, not much is known about the base index selection method in breeding for drought tolerance. Consequently, the present study has been conducted to: 1) evaluate the yield performance of cowpea genotypes under artificial drought and well-watered condition;2) develop a base index using multiple traits for ranking genotype performance. The experiment was a 25 × 2 factorial laid out in a Randomized Complete Block Design (RCBD) with three replications. The experiment was carried out in the screen house at the Department of Horticulture at KNUST. The result showed that KPR1-96-73, Simbo, CZ06-4-16, Wilibaly and Agyenkwa were high yielding in well-water condition while Ghana Shoba, Sangaraka, NKetewade, Ghana-Shoni and Korobalen were high yielding genotypes in water stress condition. The average yield reduction was 60.6% for grain respectively. The biplot displays revealed four groups among the genotypes tested which was based on their yielding capacity and drought tolerance. In cluster B high yielding and drought tolerant genotypes were identified, high yielding and drought susceptible have been identified in cluster A, low yielding and drought tolerant in cluster D, and lastly low yielding and drought susceptible in cluster C. Genotypes in cluster B, were the best due to the fact that it combines high yield and tolerance to drought. They were Ghana Shoni, Nketewade, Sangaraka and Ghana shoba. These genotypes might be suitably employed in further drought tolerance breeding program of cowpea. 展开更多
关键词 Cowpea [(vigna unguiculata (L.)] Post Flowering Drought Drought Tolerance Index
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Tagging and Utilization Bruchid Resistance Gene Using PCR Markers in Mungbean 被引量:3
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作者 CHENG Xu-zhen WANG Su-hua +3 位作者 WU Shao-yu ZHOU Ji-hong WANG Shu-min Charles Y Yang 《Agricultural Sciences in China》 CAS CSCD 2005年第8期579-583,共5页
Sixteen mungbean lines were analyzed using 56 random primers. Different DNA bands were detected between Bruchid resistant lines and susceptible lines. According to the cluster results, the 16 lines can be divided into... Sixteen mungbean lines were analyzed using 56 random primers. Different DNA bands were detected between Bruchid resistant lines and susceptible lines. According to the cluster results, the 16 lines can be divided into four groups, including brucid resistant wild types, resistant cultivated lines, resistant progenies and a mixed group. BSA method was used to identify DNA markers that related with bruchid resistant gene by using resistant line and susceptible line and their F2 progeny. One codominant marker was identified, which generated a fragment of 1.79 kb in resistant lines and 1.03 kb in susceptible lines. Finally, this codominant marker was considered to be tightly linked with bruchid resistant gene and could be useful in resistant germplasm identification and marker-assisted selection. 展开更多
关键词 Mungbean [vigna radiata(L.) Wilclzek] Bruchid resistant gene DNA marker
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Genetic diversity assessment of a set of introduced mung bean accessions(Vigna radiata L.) 被引量:3
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作者 Lixia Wang Peng Bai +4 位作者 Xingxing Yuan Honglin Chen Suhua Wang Xin Chen Xuzhen Cheng 《The Crop Journal》 SCIE CAS CSCD 2018年第2期207-213,共7页
Genetic resources from other countries or regions play an important role in broadening the genetic background of local breeding varieties. Here we describe observations on the adaptability of mung bean germplasm obtai... Genetic resources from other countries or regions play an important role in broadening the genetic background of local breeding varieties. Here we describe observations on the adaptability of mung bean germplasm obtained from the United States Department of Agriculture and their genetic diversity assessment using SSR markers. Several accessions were shown to be mixtures,based on their phenotypes for some characters. Most accessions were able to complete their lifecycles when grown in Beijing, China, making them ideal for crossbreeding without day length control. High diversity was revealed by the SSR markers, with an average of 4.2 alleles per locus and a PIC value of 0.650 per locus. STRUCTURE analysis divided the accessions into six groups.There was no obvious trend of accessions forming groups according to their geographical origin,owing mainly to germplasm exchange and an uneven distribution of accessions. The present results indicate that this germplasm would enrich the local gene pool, and provide information for the further use of germplasm in breeding programs. 展开更多
关键词 vigna RADIATA Plant introduction SSR MARKERS GENETIC diversity
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小豆远缘杂交研究进展 被引量:5
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作者 徐宁 王明海 +2 位作者 包淑英 王桂芳 郭中校 《植物遗传资源学报》 CAS CSCD 北大核心 2010年第6期666-670,共5页
小豆是豇豆属下重要的食用豆类之一。本文介绍了豇豆属下亚洲豇豆亚属的植物学分类情况,综述了小豆与其近缘种(包括栽培种、野生种)杂交的研究进展,为豇豆属特别是亚洲豇豆亚属丰富的遗传资源应用于小豆育种工作提供参考。
关键词 小豆 亚洲豇豆亚属 豇豆属 远缘杂交
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Olfactory Response of Trichogramma ostriniae (Hymenoptera: Trichogrammatidae) to Volatiles Emitted by Mungbean Plants 被引量:2
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作者 BAI Shu-xiong WANG Zhen-ying +1 位作者 HE Kang-lai Im Dae-joon 《Agricultural Sciences in China》 CAS CSCD 2011年第4期560-565,共6页
Intercropping maize with the prostrate variety of mungbean, Vigna radiata (L.) Wilczek which grows flat along the ground can increase parasitism of the eggs of the Asian corn borer, Ostrinia furnacalis (Guenée... Intercropping maize with the prostrate variety of mungbean, Vigna radiata (L.) Wilczek which grows flat along the ground can increase parasitism of the eggs of the Asian corn borer, Ostrinia furnacalis (Guenée), a crucial insect pest of maize in China, by Trichogramma ostriniae Pang Chen. This study investigated the behavioural responses of T. ostriniae to plant volatiles emitted from whole plants, leaves, and flowers of prostrate and erect varieties of mungbean in a 4-armed olfactometer. Female parasitiods were significantly attracted to the odours from some varieties of prostrate mungbean when the visit duration and the number of visits were considered, but were not attracted to the odours from any erect varieties. The attractive odours were shown to emanate from the leaves, rather than from the flowers of prostrate mungbean plants. These results have implications for the augmentation of Trichogramma parasitoids in bio-control of O. furnacalis and other crop pests. 展开更多
关键词 Trichogramma ostriniae olfactory response vigna radiata plant volatile
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Draft genome sequence of a less-known wild Vigna: Beach pea(V. marina cv. ANBp-14-03) 被引量:2
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作者 Awnindra Kumar Singh A.Velmurugan +8 位作者 Debjyoti Sen Gupta Jitendra Kumar Ravi Kesari Aravind Konda Narendra Pratap Singh Sibnarayan Dam Roy Utpal Biswas R.Rahul Kumar Sanjay Singh 《The Crop Journal》 SCIE CAS CSCD 2019年第5期660-666,共7页
Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blac... Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blackgram(V.mungo),and is distributed throughout the tropics.With its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna species.However,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were designed.Genome analysis identified 50,670 genes with mean coding sequence length 1042 bp.Phylogenetic analysis revealed highest sequence similarity with V.angularis,followed by V.radiata.Comparison with the V.angularis genome revealed 16,699 SNPs and 2253 InDels and comparison with the V.radiata genome revealed 17,538 SNPs and 2300 InDels.To our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of India.The draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species. 展开更多
关键词 BEACH PEA vigna MARINA NextSeq 500 WHOLE-GENOME sequencing Salinity tolerance
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Preliminary Study on Geographical Distribution and Evolutionary Relationships Between Cultivated and Wild Adzuki Bean (Vigna angular is var. angularis and var. nipponensis) by AFLP Analysis 被引量:1
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作者 ZONGXu-xiao DuncanVaughan +4 位作者 NorihikoTomooka AkitoKaga WANGXin-wang GUANJian-ping WANGShu-min 《Agricultural Sciences in China》 CAS CSCD 2003年第4期368-376,共9页
A set of representative 146 adzuki (Vigna angular is var. angularis, and var. nipponensis) germplasm from 6 Asian countries traditionally for adzuki bean production, together with an out group standard rice bean (Vign... A set of representative 146 adzuki (Vigna angular is var. angularis, and var. nipponensis) germplasm from 6 Asian countries traditionally for adzuki bean production, together with an out group standard rice bean (Vigna umbellata), were analyzed by AFLP methodology using 12 informative primer pairs. 313 unambiguous polymorphic bands were created. According to the dendrogram by cluster analysis based on AFLP banding, 143 of the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Vigna angularis. A neighbor joining tree was generated using newly developed Innan's nucleotide diversity estimate from the AFLP data. From analysis, 7 distinct evolutionary groups, named as "Chinese cultivated", "Japanese cultivated", "Japanese complex-Korean cultivated", "Chinese wild", "China Taiwan wild", "Nepal-Bhutan cultivated" and "Hymalayan wild", were detected. Nucleotide diversity with geographical distribution of each group is discussed, regarding the evolutionary relationships between wild and cultivated adzuki beans. The preliminary results indicated that cultivated adzuki bean should be domesticated from at least 4 progenitors in at least 3 geographical origins. 展开更多
关键词 Adzuki bean (vigna angularis) AFLP Genetic diversity Geographical distribution Evolutionary relationships
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