"科学、技术、工程与数学"(Science,Technology,Engineering and Mathematics,简称STEM)教育是由美国发起、主导并正在兴盛于世界各国的一场教育运动。它的提出,主要因应于国际竞争、社会高科技的发展等对人才的需求。这决定..."科学、技术、工程与数学"(Science,Technology,Engineering and Mathematics,简称STEM)教育是由美国发起、主导并正在兴盛于世界各国的一场教育运动。它的提出,主要因应于国际竞争、社会高科技的发展等对人才的需求。这决定了其功利主义特点。因此,当我们思考STEM的教育价值时,必须将其功利主义框架转化为知识创造框架,追寻STEM的知识价值和教育内在价值。在此意义上,先于STEM而产生的科学、技术与社会(Science,Technology and Society,简称STS)教育,更具学术的背景和基础,可以作为改造STEM教育的合理框架。此外,我们还应深入探究STEM各相关学科自身的价值,并在此基础上寻求它们之间相互融合的价值和可能。展开更多
The gene Pi15 for resistance of rice to Magnaporthe grisea was previously identified as being linked to the gene Pii. However, there is a debate on the chromosomal position of the Pii gene, because it was originally m...The gene Pi15 for resistance of rice to Magnaporthe grisea was previously identified as being linked to the gene Pii. However, there is a debate on the chromosomal position of the Pii gene, because it was originally mapped on chromosome 6, but recent work showed it might be located on chromosome 9. To determine the chromosomal location of the Pi15 gene, a linkage analysis using molecular markers was performed in a F2 mapping population consisting of 15 resistant and 141 susceptible plants through bulked-segregant analysis (BSA) in combination with recessive-class analysis (RCA). Out of 20 microsatellite markers mapped on chromosomes 6 and 9 tested, only one marker, RM316 on chromosome 9, was found to have a linkage with the Pi15 gene with a recombination frequency of (19.1 ±3.7)%. To confirm this finding, four sequence-tagged site (STS) markers mapped on chromosome 9 were tested. The results suggested that marker G103 was linked to the Pi15 gene with a recombination frequency of (5.7±2.1)%. To find marker(s) more closely linked to the Pi15 gene, random amplified polymorphic DNA (RAPD) analysis was performed. Out of 1 000 primers tested, three RAPD markers, BAPi15486, BAPi15782 and BAPil5844 were found to tightly flank the Pi15 gene with recombination frequencies of 0.35%, 0.35% and 1.1%, respectively. These three RAPD markers should be viewed as the starting points for marker-aided gene pyramiding and cloning. A new gene cluster of rice blast resistance on chromosome 9 was also discussed.展开更多
文摘"科学、技术、工程与数学"(Science,Technology,Engineering and Mathematics,简称STEM)教育是由美国发起、主导并正在兴盛于世界各国的一场教育运动。它的提出,主要因应于国际竞争、社会高科技的发展等对人才的需求。这决定了其功利主义特点。因此,当我们思考STEM的教育价值时,必须将其功利主义框架转化为知识创造框架,追寻STEM的知识价值和教育内在价值。在此意义上,先于STEM而产生的科学、技术与社会(Science,Technology and Society,简称STS)教育,更具学术的背景和基础,可以作为改造STEM教育的合理框架。此外,我们还应深入探究STEM各相关学科自身的价值,并在此基础上寻求它们之间相互融合的价值和可能。
文摘The gene Pi15 for resistance of rice to Magnaporthe grisea was previously identified as being linked to the gene Pii. However, there is a debate on the chromosomal position of the Pii gene, because it was originally mapped on chromosome 6, but recent work showed it might be located on chromosome 9. To determine the chromosomal location of the Pi15 gene, a linkage analysis using molecular markers was performed in a F2 mapping population consisting of 15 resistant and 141 susceptible plants through bulked-segregant analysis (BSA) in combination with recessive-class analysis (RCA). Out of 20 microsatellite markers mapped on chromosomes 6 and 9 tested, only one marker, RM316 on chromosome 9, was found to have a linkage with the Pi15 gene with a recombination frequency of (19.1 ±3.7)%. To confirm this finding, four sequence-tagged site (STS) markers mapped on chromosome 9 were tested. The results suggested that marker G103 was linked to the Pi15 gene with a recombination frequency of (5.7±2.1)%. To find marker(s) more closely linked to the Pi15 gene, random amplified polymorphic DNA (RAPD) analysis was performed. Out of 1 000 primers tested, three RAPD markers, BAPi15486, BAPi15782 and BAPil5844 were found to tightly flank the Pi15 gene with recombination frequencies of 0.35%, 0.35% and 1.1%, respectively. These three RAPD markers should be viewed as the starting points for marker-aided gene pyramiding and cloning. A new gene cluster of rice blast resistance on chromosome 9 was also discussed.
