Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and t...Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each sig-nificant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=?1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.展开更多
This paper investigates a simple approach proposed towards performance-based earthquake engineering (PBEE) which has potential applications to the performance-based design (PBD) and performance-based assessment (PBA) ...This paper investigates a simple approach proposed towards performance-based earthquake engineering (PBEE) which has potential applications to the performance-based design (PBD) and performance-based assessment (PBA) fields. The simple method of PBEE encompasses three areas of seismic risk which include seismic hazard, structural analysis, and loss models. The aim of the PBEE process, entitled as FEMA P-58, is to present essential data needed to make a rational decision regarding predicted performance, where various sources of uncertainties are involved. In developing countries, the lack of suitable real ground motions corresponding to site characteristics and seismicity particularly for larger intensities and the scarcity of demands, which makes it hard to identify the seismic capacity of a structure, is the main our motivation of using the FEMA method. In this paper, the method of FEMA P-58 is investigated, in terms of available tools and required data, in such a way that it will be applicable for developing countries which are located in high seismic hazard zones. To achieve this goal, three steel moment-resisting buildings with low and high ductility, and three steel braced-frame buildings are selected as case studies. The mean annual loss is estimated by the available software, Performance Assessment Calculation Tool (PACT). The achieved results, i.e. the loss curves, will provide a simple means by which the engineers can quantify and communicate seismic performance to other stakeholders. In the case study buildings, the braced one has less annual losses in comparison with other investigated cases, and the structure with high ductility can be considered as the next ones. Execution cost of each building should be considered by contractors. Also, seismic fragility curves of structures for various limit states, as well, the corresponding loss models are identified as the most essential data towards application of the investigated PBEE process.展开更多
An asymptotic existence of balanced incomplete block (BIB) designs and pairwise balanced designs (PBD) has been discussed in [1]-[3]. On the other hand, the existence of additive BIB designs and pairwise additive BIB ...An asymptotic existence of balanced incomplete block (BIB) designs and pairwise balanced designs (PBD) has been discussed in [1]-[3]. On the other hand, the existence of additive BIB designs and pairwise additive BIB designs with k = 2?and?λ = 1?has been discussed with direct and recursive constructions in [4]-[8]. In this paper, an asymptotic existence of pairwise additive BIB designs is proved by use of Wilson’s theorem on PBD, and?also for some l?and k the exact existence of l?pairwise additive BIB designs with block size k and?λ = 1?is discussed.展开更多
To acquire human operation skill based on force sense, element contact form (ECF) is proposed to describe contact condition firstly. The skill is modeled as a sequence of discrete ECFs. Since different ECF has differe...To acquire human operation skill based on force sense, element contact form (ECF) is proposed to describe contact condition firstly. The skill is modeled as a sequence of discrete ECFs. Since different ECF has different force distribution, a support vector machine classifier is built to identify the contact conditions according to the force signal. Finally, the robot can obtain the skill from the human demonstration.展开更多
基金Project (No. 2004C32049) supported by the Science and Technology Department of Zhejiang Province, China
文摘Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each sig-nificant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=?1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.
文摘This paper investigates a simple approach proposed towards performance-based earthquake engineering (PBEE) which has potential applications to the performance-based design (PBD) and performance-based assessment (PBA) fields. The simple method of PBEE encompasses three areas of seismic risk which include seismic hazard, structural analysis, and loss models. The aim of the PBEE process, entitled as FEMA P-58, is to present essential data needed to make a rational decision regarding predicted performance, where various sources of uncertainties are involved. In developing countries, the lack of suitable real ground motions corresponding to site characteristics and seismicity particularly for larger intensities and the scarcity of demands, which makes it hard to identify the seismic capacity of a structure, is the main our motivation of using the FEMA method. In this paper, the method of FEMA P-58 is investigated, in terms of available tools and required data, in such a way that it will be applicable for developing countries which are located in high seismic hazard zones. To achieve this goal, three steel moment-resisting buildings with low and high ductility, and three steel braced-frame buildings are selected as case studies. The mean annual loss is estimated by the available software, Performance Assessment Calculation Tool (PACT). The achieved results, i.e. the loss curves, will provide a simple means by which the engineers can quantify and communicate seismic performance to other stakeholders. In the case study buildings, the braced one has less annual losses in comparison with other investigated cases, and the structure with high ductility can be considered as the next ones. Execution cost of each building should be considered by contractors. Also, seismic fragility curves of structures for various limit states, as well, the corresponding loss models are identified as the most essential data towards application of the investigated PBEE process.
文摘An asymptotic existence of balanced incomplete block (BIB) designs and pairwise balanced designs (PBD) has been discussed in [1]-[3]. On the other hand, the existence of additive BIB designs and pairwise additive BIB designs with k = 2?and?λ = 1?has been discussed with direct and recursive constructions in [4]-[8]. In this paper, an asymptotic existence of pairwise additive BIB designs is proved by use of Wilson’s theorem on PBD, and?also for some l?and k the exact existence of l?pairwise additive BIB designs with block size k and?λ = 1?is discussed.
文摘To acquire human operation skill based on force sense, element contact form (ECF) is proposed to describe contact condition firstly. The skill is modeled as a sequence of discrete ECFs. Since different ECF has different force distribution, a support vector machine classifier is built to identify the contact conditions according to the force signal. Finally, the robot can obtain the skill from the human demonstration.
