OBJECTIVE:To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea(PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in ...OBJECTIVE:To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea(PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in the light of skin temperature.METHODS:Fifty-two PD patients and 49 healthy volunteers were recruited.Skin temperature measurements were performed with a skin temperature assessment device at 10 points.Absolute difference between skin temperature of the same point on the left and right side is used as main outcome measure.RESULTS:On the first day of menstruation, when menstrual pain attacking in PD patients, a significant increase in skin temperature difference was detected at Taixi(KI 3) compared with the healthy group(P < 0.01).A significant reduction in skin temperature difference was detected at Taixi(KI 3) in the first day of menstruation compared with those values in the third day after menstruation(P < 0.01)in the healthy group.On the third day after menstruation, a significant reduction in skin temperature difference was found at Zhongdu(LR 6) in PD group compared with the healthy group(P < 0.05).No significant differences of skin temperature were detected at other points(P > 0.05).CONCLUSION:The skin temperature difference at menstruation-relevant points in PD patients did not all change significantly more than those in women without PD.Significant difference was only found in Taixi(KI 3), the Yuan-source point of Kidney meridian.展开更多
Hegu （LI 4） and Taichong （LR 3） are the Yuan- Primary acupoints of the Large Intestine and Liver Meridian, and are collectively named as Si Guan （four gates） point. Combined use of these two acupoints can harmon...Hegu （LI 4） and Taichong （LR 3） are the Yuan- Primary acupoints of the Large Intestine and Liver Meridian, and are collectively named as Si Guan （four gates） point. Combined use of these two acupoints can harmonize yin and yang, regulate qi and blood, and balance ascending or descending. This paper attempts to study the effects of Si Guan point on the vascular dilation and constriction in migraine patients from the perspective of its feature.展开更多
Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobac...Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.展开更多
Objective:To compare therapeutic effects of acupuncture at Ligou(LR 5) plus movement therapy and conventional acupuncture on cervical spondylosis.Methods:The therapeutic effect of acupuncture at Ligou(LR 5) plus movem...Objective:To compare therapeutic effects of acupuncture at Ligou(LR 5) plus movement therapy and conventional acupuncture on cervical spondylosis.Methods:The therapeutic effect of acupuncture at Ligou(LR 5) plus movement therapy on 57 cases of cervical spondylosis(Group Acup.+M) was observed and compared with that of conventional acupuncture(Group Acup.) on 65 cases of cervical spondylosis with the same types of the disease during the same observation period as those treated by the former therapy.Results:The cured rate,effective rate and total effective rate were 52.63%,45.61% and 98.24%,respectively in Group Acup.+M;46.15%,50.77% and 96.92%,respectively in Group Acup.There were no significant differences in therapeutic effects between the two groups,shown by Ridit test(P>0.05).Conclusion:Both acupuncture plus movement therapy and conventional acupuncture were similarly effective in treating cervical spondylosis,but the former was superior to the latter in shorter treatment course and fewer points used.展开更多
Some existed fuzzy regression methods have some special requirements for the object of study, such as assuming the observed values as symmetric triangular fuzzy numbers or imposing a non-negative constraint of regress...Some existed fuzzy regression methods have some special requirements for the object of study, such as assuming the observed values as symmetric triangular fuzzy numbers or imposing a non-negative constraint of regression parameters. In this paper, we propose a left-right fuzzy regression method, which is applicable to various forms of observed values. We present a fuzzy distance and partial order between two left-right (LR) fuzzy numbers and we let the mean fuzzy distance between the observed and estimated values as the mean fuzzy error, then make the mean fuzzy error minimum to get the regression parameter. We adopt two criteria involving mean fuzzy error (comparative mean fuzzy error based on partial order) and SSE to compare the performance of our proposed method with other methods. Finally four different types of numerical examples are given to illustrate that our proposed method has feasibility and wide applicability.展开更多
基金Supported by the National Basic Research Program of China(973 Program)-the Effect of De Qi on Acupoint Specific Effect Based on Meridians and its Characteristics and Molecular Response Mechanisms(No.2012CB518506)Research on Acupoint Specificity in Regulating Uterus(No.2006CB504503)National Natural Science Foundation of China-Research on Variations of Biophysical Properties of Acupoints on Different Meridians with the Same Spinal Segments Based on Primary Dysmenorrhea(No.81573884)
文摘OBJECTIVE:To assess skin temperature response to menstruation at acupuncture points in primary dysmenorrhea(PD) patients and healthy volunteers so as to explore acupuncture point specificity in reflecting diseases in the light of skin temperature.METHODS:Fifty-two PD patients and 49 healthy volunteers were recruited.Skin temperature measurements were performed with a skin temperature assessment device at 10 points.Absolute difference between skin temperature of the same point on the left and right side is used as main outcome measure.RESULTS:On the first day of menstruation, when menstrual pain attacking in PD patients, a significant increase in skin temperature difference was detected at Taixi(KI 3) compared with the healthy group(P < 0.01).A significant reduction in skin temperature difference was detected at Taixi(KI 3) in the first day of menstruation compared with those values in the third day after menstruation(P < 0.01)in the healthy group.On the third day after menstruation, a significant reduction in skin temperature difference was found at Zhongdu(LR 6) in PD group compared with the healthy group(P < 0.05).No significant differences of skin temperature were detected at other points(P > 0.05).CONCLUSION:The skin temperature difference at menstruation-relevant points in PD patients did not all change significantly more than those in women without PD.Significant difference was only found in Taixi(KI 3), the Yuan-source point of Kidney meridian.
基金Scientific Research Foundation of Zhejiang Education Department (Y200805946)Open Foundation of Top Priority Projects of Zhejiang Universities (Acupuncturemoxibustion and Tuina) (ZJZZ-0902)
文摘Hegu （LI 4） and Taichong （LR 3） are the Yuan- Primary acupoints of the Large Intestine and Liver Meridian, and are collectively named as Si Guan （four gates） point. Combined use of these two acupoints can harmonize yin and yang, regulate qi and blood, and balance ascending or descending. This paper attempts to study the effects of Si Guan point on the vascular dilation and constriction in migraine patients from the perspective of its feature.
基金supported by the National Natural Science Foundation of China (No. 21177009)
文摘Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, the biodegradation is found to be the most efficient method for removing microcystins 0VICs） produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, we for the first time cloned and expressed a gene USTB-O5-A （HM245411） that is responsible for the first step in the biodegradation of microcystin LR （MC-LR） in E. coli DH5ct, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1, respectively. The cell-free extracts （CE） of recombinant E. coli DH5ct containing USTB-O5-A had high activity for biodegrading MC-LR. The initial MC-LR concentration of 40 mg/L was completely biodegraded within 1 hr in the presence of CE with a protein concentration of 0.35 mg/mL. Based on an analysis of the liquid chromatogram-mass spectrum （LC-MS）, the enzyme encoded by gene USTB-O5-A was found to be active in cleaving the target peptide bond between 3-amino-9-methoxy-2,6, 8-trimethyl-10-phenyl-deca-4,6-dienoic acid （Adda） and arginine of MC-LR, and converting cyclic MC-LR to linear MC-LR as a first product that is much less toxic than parent MC-LR, which offered direct evidence for the first step on the pathway of MC-LR biodegradation by Sphingopyxis sp. USTB-05.
基金supported by a grant from National Natural Science Foundation of China (No 30772839, No30873302)
文摘Objective:To compare therapeutic effects of acupuncture at Ligou(LR 5) plus movement therapy and conventional acupuncture on cervical spondylosis.Methods:The therapeutic effect of acupuncture at Ligou(LR 5) plus movement therapy on 57 cases of cervical spondylosis(Group Acup.+M) was observed and compared with that of conventional acupuncture(Group Acup.) on 65 cases of cervical spondylosis with the same types of the disease during the same observation period as those treated by the former therapy.Results:The cured rate,effective rate and total effective rate were 52.63%,45.61% and 98.24%,respectively in Group Acup.+M;46.15%,50.77% and 96.92%,respectively in Group Acup.There were no significant differences in therapeutic effects between the two groups,shown by Ridit test(P>0.05).Conclusion:Both acupuncture plus movement therapy and conventional acupuncture were similarly effective in treating cervical spondylosis,but the former was superior to the latter in shorter treatment course and fewer points used.
文摘Some existed fuzzy regression methods have some special requirements for the object of study, such as assuming the observed values as symmetric triangular fuzzy numbers or imposing a non-negative constraint of regression parameters. In this paper, we propose a left-right fuzzy regression method, which is applicable to various forms of observed values. We present a fuzzy distance and partial order between two left-right (LR) fuzzy numbers and we let the mean fuzzy distance between the observed and estimated values as the mean fuzzy error, then make the mean fuzzy error minimum to get the regression parameter. We adopt two criteria involving mean fuzzy error (comparative mean fuzzy error based on partial order) and SSE to compare the performance of our proposed method with other methods. Finally four different types of numerical examples are given to illustrate that our proposed method has feasibility and wide applicability.
