Gluconeogenesis responses was assessed during a short starvation period and subsequent refeeding in Siberian sturgeon(Acipenser baerii) previously fed different dietary carbohydrates levels and experienced to a glucos...Gluconeogenesis responses was assessed during a short starvation period and subsequent refeeding in Siberian sturgeon(Acipenser baerii) previously fed different dietary carbohydrates levels and experienced to a glucose stimuli during early life. The sturgeon larvae were previously fed either a high glucose diet(G) or a low glucose diet(F) from the first feeding to yolk absorption(8 to 12 d post-hatching [dph]). Each group of fish was sub-divided into 2 treatments at 13 dph and was fed either a high-carbohydrate diet(H) or a low carbohydrate diet(L) until 20 wk. In the current study, the fish in 4 groups(GL, FL, GH and FH) were experienced to starvation for 21 d following by re-feeding of their corresponding diets for 21 d.Fish were sampled at postprandial 6 and 24 h before starvation(P6 h and P24 h), starvation 7,14 and 21 d(S7, S14 and S21) and 1, 7,14 and 21 d during refeeding(R1, R7, R14 and R21). Plasma samples during refeeding were taken at P6 h at each time point. Glycaemia levels, liver and muscle glycogen contents,activities and mRNA levels of hepatic gluconeogenic enzymes were examined. We found that both dietary carbohydrate levels and early glucose stimuli significantly affected the metabolic responses to starvation and refeeding in Siberian sturgeon(P < 0.05). During prolonged starvation, Siberian sturgeon firstly mobilized the liver glycogen and then improved gluconeogenesis when the dietary carbohydrates were abundant, whereas preserved the liver glycogen stores at a stable level and more effectively promoted gluconeogenesis when the dietary carbohydrates are absent to maintain glucose homoeostasis.During refeeding, as most teleostean, Siberian sturgeon failed controlling the activities and mRNA levels of phosphoenolpyruvate carboxykinase cytosolic forms(PEPCK-C), fructose-1,6-bisphosphatase(FBPase),but particularly controlled phosphoenolpyruvate carboxykinase mitochondrial forms(PEPCK-M) activities and mRNA expression of glucose-6-phosphatase(G6 Pase, except in GL group). Siberian sturgeon has a fu展开更多
The Chinese sturgeon （Acipenser Sinensis） is one of the unique and important fishery resources in China. Since the construction of the Gezhouba Dam, the traditional migration route of the sturgeon has been blocked; ...The Chinese sturgeon （Acipenser Sinensis） is one of the unique and important fishery resources in China. Since the construction of the Gezhouba Dam, the traditional migration route of the sturgeon has been blocked; consequently, the length of natural spawning sites is reduced from 800 km in the past to less than 5 km at present. As an endangered species, the Chinese sturgeon has become one of the most conserved aquatic species. In this article, the flow field of its spawning states in the downstream of Gezhouba Dam was simulated and analyzed using N-S equations and k-ε turbulence model. Volume Of Fluid （VOF） method with the Finite Volume Method （FVM） was used to simulate the water-air two-phase flow to examine the computed area. On the basis of the ecological-hydraulic characteristics of Chinese sturgeon, the features of the flow field were investigated to provide theoretical support for the proper management of the Three Gorges Reservoir.展开更多
Gezhouba Dam was built in 1981 across the Yangtze River at Yichang, Hubei Prov-ince, China. It blocked the upstream spawning migration of all anadromous species offish, including the Chinese sturgeon Acipenser sinensi...Gezhouba Dam was built in 1981 across the Yangtze River at Yichang, Hubei Prov-ince, China. It blocked the upstream spawning migration of all anadromous species offish, including the Chinese sturgeon Acipenser sinensis. Although the main historicalspawing areas of Chinese sturgeons were far upstream of the dam, successful spawning inthe reach below the dam has been documented each year since 1982 by collecting展开更多
We conducted experiments in an artificial stream tank with wild juvenile Chinese sturgeon Acipenser sinensis captured in the Yangtze Estuary to test the null hypothesis that their feeding efficiency on Barcheek goby R...We conducted experiments in an artificial stream tank with wild juvenile Chinese sturgeon Acipenser sinensis captured in the Yangtze Estuary to test the null hypothesis that their feeding efficiency on Barcheek goby Rhinogobius giurinus was not affected by sand vs. smooth glass substrate. Gobies are among the most common prey eaten by wild juvenile A. sinensis in the estuary. Test results found neither substrate type significantly affected feeding efficiency by juveniles. Previous research found a strong innate habitat preference of A. sinensis for sand substrate. The present results indicate that the preference for sand is not related to efficiently capturing R. giurinus on sand, but is an adaptation predisposing juvenile A. sinensis to seek sandy substrate where R. giurinus and other benthic forage are the most abundant in the Yangtze Estuary.展开更多
The paper presents the results of the sterlet sturgeon Acipenser ruthenus reintroduction into the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries into the reservoir since 2001.The effectiveness of hatch...The paper presents the results of the sterlet sturgeon Acipenser ruthenus reintroduction into the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries into the reservoir since 2001.The effectiveness of hatchery releases was evaluated by monitoring gillnet sampling 2014-2020.The results showed that the species was reported throughout entire Kama Reservoir,which contrasts with 1988-1994 data,when the species was registered only at the upper part of the reservoir near the Kama River mouth.The highest number and biomass values of catch per unit effort(CPUE)of the sterlet were found in submerged river channels-the deepest habitats with flow conditions which are close to the ecological requirements of the species.The distribution of the sterlet within the channel biotopes of the reservoir is defined by reduced variability.The biggest individuals and the widest size range of the sterlet were typical for the upper area of the reservoir and Chusovskoy Bay,which have the greatest flow.The smallest sizes were typical for fish from catches near the dam of the reservoir,which is consistent with the published data on the distribution of sterlet in reservoir conditions.The presented results may indicate the artificial formation of the sterlet stock in the Kama Reservoir maintained by hatchery releases.The revealed distribution of the sterlet in the reservoir determines the direction of further research on natural reproduction and thus formation of an artificially formed population.Also,future studies are required to assess the possibility of hybridization of the sterlet of artificial origin with natural populations of the species,since the low genetic diversity of the sterlet released into the Kama Reservoir which can lead to irreversible loss of genetic individuality of the natural stocks of the species in the catchment of upper course of the Kama River.展开更多
[Objective]This study was to investigate the effect of different concentrations of Norfloxacin on the superoxide dismutase （ SOD） activities in the blood plasma and liver tissues of Amur sturgeon （ Acipenser schren...[Objective]This study was to investigate the effect of different concentrations of Norfloxacin on the superoxide dismutase （ SOD） activities in the blood plasma and liver tissues of Amur sturgeon （ Acipenser schrencki Brandt） and sterlet （ Acipenser ruthenus Linnaeus） . [Method] Using pharmaco-toxicological evaluation method,Norfloxacin with the concentrations of 0,20,40,60,80 and 100 mg /kg,was orally delivered to the Amur sturgeon and sterlet for 5 d,respectively. The SOD activities in the blood plasma and liver tissues were measured after drug withdrawal for 2 d to explore the optimal dosing concentration of Norfloxacin during sturgeon culture,as well as the effect of Norfloxacin on liver injury. [Result] SOD existed in both two sturgeons but with different amounts,and the SOD activities were higher in the livers than in the blood plasma no matter in the control and all drug delivered groups. Under different drug delivery concentrations,the SOD activities first increased and then decreased in the 2 tissues of the 2 sturgeon species,and the SOD activities reached the maximum when the drug delivery concentration was 40 mg /kg. The drug de- livery concentration had little effect on the SOD activities in the blood plasma,which showed stable changes. When the drug delivery concentration was 40 mg /kg,the SOD activities in the blood plasma of Amur sturgeon were higher than that of sterlet,while for the other concentrations,the SOD activities in the plasma of sterlet showed higher performance. However,the SOD activities changed significantly through drug delivery in the liver tis- sues,and the SOD activities were higher in the sterlet than in the Amur sturgeon when the drug delivery concentrations were 0,40 and 100 mg /kg. The SOD activity in the sterlet was the highest under 40 mg /kg,presenting a sharp peak value. The optimal drug delivery concentration of Norfloxa- cin was 30 -50 mg /kg,under which the Norfloxacin presented best effect and had no injury effect on livers. [Conclusion]This study provides展开更多
Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></spa...Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></span><span style="font-family:Verdana;">) is a primitive freshwater fish and a source of black caviar.</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">The genes involved in sexual determination and differentiation are still unknown and there are no molecular markers for sex identification in this species. Studying the variation of the sex-based differences in genomic sequences and in gene expression in the sturgeon may lead to markers of sex in early stages of development and advances in aquaculture, as well as provide novel insights about the evolution of reproduction, sex determination, and sexual differentiation mechanisms in vertebrates. Previous studies by our and other groups have identified differentially expressed genes in the gonads of adult female and male sturgeon. The current study aimed to test whether these ge</span><span style="font-family:Verdana;">nes were also differentially expressed in non-gonadal tissue, namely fins. We measured by qRT-PCR the mRNA levels of 29 known and novel sex-related genes in the gonads and fins of males (4 years old) and females (7 years old;sexual maturation is earlier in males than in females). Six genes (ATP6, IGFRM, LIA1A, S1A, NPL1A, GAPDH and SOX9) showed higher expression in female fin</span><span style="font-family:Verdana;">s. However, only ATP6 mRNA levels differed in fins of males and females of the same age (4 years old). These findings underscore the impracticality of sex identification based on gene expression in non-gonadal</span><span style="font-family:Verdana;"> tissue and the need for genetic sex markers in the Russian sturgeon.展开更多
The development of new agricultural industries is an important challenge for a region’s economic establishment. The connection between a research department at MIGAL Institute and biotechnology in aquaculture is an e...The development of new agricultural industries is an important challenge for a region’s economic establishment. The connection between a research department at MIGAL Institute and biotechnology in aquaculture is an example (case study) of the interrelationships that also exist in other departments. This article describes the research conducted by the institute supporting the development of the aquaculture industry in Israel through the introduction of a relatively new fish species—the Russian sturgeon (Acipenser gueldenstaedtii), with the relevant information to be used for acclimatization to aquaculture conditions in northern Israel. Many aspects related to bringing a new species to Israel and working with this new industry to study its adaptation to existing conditions in the north of the country influenced the economic success of this new type of precision agriculture. This article describes the research on various aspects affecting the successful acclimatization of Russian sturgeon in northern Israel: introduction, food development, reproduction, hormone systems that control reproduction and growth, gonadal development, and molecular markers for sex determination, differentiation, and economic assessment of caviar production.展开更多
[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base...[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.展开更多
In the White sturgeon fish farms, some individuals have difficulty in getting access to food: such sturgeons are called "runts", and they result in a slower growth rate than normally feeding fish. In this pa...In the White sturgeon fish farms, some individuals have difficulty in getting access to food: such sturgeons are called "runts", and they result in a slower growth rate than normally feeding fish. In this paper, we have studied the gut peculiarities of runt sturgeons. Utilizing in paralleling an analysis of diatom populations in both the fish gut tissues and the rearing tank waters, we hypothesized a causative relation between the occurrence of runt sturgeons and periodic diatom blooms. In fact, we have observed that the diatom species identified in the aquatic environment were also detected in organs (Fragilaria spp and Rhoicosfenia spp for both glandular body, mid-intestine) of the runt sturgeon's gut, but not in tissues of normally feeding individuals. Owing to their siliceous wall, diatoms can be responsible for areas of epithetlial detachment in the mucosal surfaces of the alimentary canal and a catharral inflammation in both the gastric pits and intestinal folds which may be the cause of secondary bacterial diseases. We suggest that diatom blooms may contribute to the occurrence of runt sturgeons in the studied Italian fish farm.展开更多
As a living fossil, the endangered Chinese sturgeon(Acipenser sinensis) has been considered a national treasure in China. Here,the famous Gezhouba Dam and Three Gorges Dam on the Yangtze River were built in 1988 and 2...As a living fossil, the endangered Chinese sturgeon(Acipenser sinensis) has been considered a national treasure in China. Here,the famous Gezhouba Dam and Three Gorges Dam on the Yangtze River were built in 1988 and 2006, for economic purposes.The natural population of Chinese sturgeon has declined since then, as these dams block its migratory route to the original spawning grounds in the middle reaches of the Yangtze River. In 2013 and 2014, there was an absence of spawning where it typically happened near the Gezhouba Dam. Nevertheless, from April to June in 2015, over 1,000 larvae with different body lengths(10–35 cm) were detected along the Shanghai Yangtze Estuary; but only little is currently known about the population genetic structure of the Chinese sturgeon. Herein, we inferred population genetic parameters from 462 available Chinese sturgeon specimens based on a 421-bp fragment of the mitochondrial DNA(mtDNA) D-loop region and 1,481,620 SNPs(singlenucleotide polymorphisms) generated by restriction site-associated DNA sequencing(RAD-seq). For the D-loop dataset, 15 haplotypes were determined. Randomly picked 23 individuals, representing the 15 D-loop haplotype groups, were subsequently used for further RAD-seq validation. The average nucleotide diversity calculated from the mtDNA and RAD datasets was 0.0086 and 0.000478, respectively. The overall effective female population size was calculated to be 1,255 to 2,607, and the long-term effective population size was estimated to range from 11,950 to 119,500. We observed that the genetic variability and the effective female population size of the current population in the Yangtze River are severely low, which are similar to the data reported over 10 years ago. The deduced relatively small effective population of female fish, limiting the genetic connectivity among Chinese sturgeon, should be considered a serious threat to this endangered species.展开更多
According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT...According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT-PCR, false sequencing was corrected, and full length eDNA of the ferritin subunit from the Chinese sturgeon was obtained. After being submitted to the GenBank database, the sequence accession number EU348782 was assigned. With the length of 896 bp, this eDNA includes entire coding regions of 53 lbp, which encodes 176 amino acids （aa）. The molecular weight was predicted to be 20339.9Mr and the theoretical isoelectric point 5.66. It shares 82.9% protein sequence homology with the ferritin of the Atlantic salmon. This gene is expressed in many organs of the Chinese sturgeon, for example, the liver, pancreas, muscle, brain, heart and gastric mucosa. The highest expression level was found in the pancreas and the heart, while the muscular tissue showed the lowest. Homology modeling was used to predict the 3-D structure of the protein, which included 5 alpha helices and 10 turns. The ferritin protein structure could be overlapped and showed high similarity with that of human, flog and bacteria. It was revealed that this kind of ferritin was highly conserved in structure and function.展开更多
[Objective] The paper was to understand the physiological, enzymatic and molecular changes of Acipenser schrencki induced by heat exposure. [Method] The effects of heat exposure on A. schrencki were investigated. The ...[Objective] The paper was to understand the physiological, enzymatic and molecular changes of Acipenser schrencki induced by heat exposure. [Method] The effects of heat exposure on A. schrencki were investigated. The temperature was increased from(18± 0.5) ℃ by 0.08 ℃/min and respectively kept at(32±0.5) ℃ for 2, 4 and 6 h. The respiration rate, related biochemical indicators and heat shock protein 70(Hsp 70) mR-NA expression were investigated. [Result] At(32±0.5) ℃, the activities of total protein, albumin, globulin, urea nitrogen, ceatinine, malondialde-hyde, aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase fluctuated dramatically from 2 h to 6 h. Meanwhile, the respi-ration rate and Hsp 70 m RNA expression in heart, liver and spleen were significantly increased from 2 h to 6 h. In comparison, the lower respira-tion rate and tiny fluctuations in activities of biochemical indicators were consistent with the lower Hsp 70 expression. [Conclusion] During the process of heat stress, A. schrencki seems to be sensitive to such temperature, and manifests generally consistent gill breathing, biochemical indica-tors and Hsp70 gene expression, implying better heat tolerance.展开更多
基金supported by the National Basic Research Program of China(2014CB138601)The National Natural Science Foundation of China(No.31572631,No.31372539)+2 种基金Beijing Technology System for Sturgeon and Salmonids(SCGWZJ 20171103-1)the National Key Research and Development Program of China(2016YFF0201900)The special Fund for Agro-Scientific Research in the Public Interest(201203015)
文摘Gluconeogenesis responses was assessed during a short starvation period and subsequent refeeding in Siberian sturgeon(Acipenser baerii) previously fed different dietary carbohydrates levels and experienced to a glucose stimuli during early life. The sturgeon larvae were previously fed either a high glucose diet(G) or a low glucose diet(F) from the first feeding to yolk absorption(8 to 12 d post-hatching [dph]). Each group of fish was sub-divided into 2 treatments at 13 dph and was fed either a high-carbohydrate diet(H) or a low carbohydrate diet(L) until 20 wk. In the current study, the fish in 4 groups(GL, FL, GH and FH) were experienced to starvation for 21 d following by re-feeding of their corresponding diets for 21 d.Fish were sampled at postprandial 6 and 24 h before starvation(P6 h and P24 h), starvation 7,14 and 21 d(S7, S14 and S21) and 1, 7,14 and 21 d during refeeding(R1, R7, R14 and R21). Plasma samples during refeeding were taken at P6 h at each time point. Glycaemia levels, liver and muscle glycogen contents,activities and mRNA levels of hepatic gluconeogenic enzymes were examined. We found that both dietary carbohydrate levels and early glucose stimuli significantly affected the metabolic responses to starvation and refeeding in Siberian sturgeon(P < 0.05). During prolonged starvation, Siberian sturgeon firstly mobilized the liver glycogen and then improved gluconeogenesis when the dietary carbohydrates were abundant, whereas preserved the liver glycogen stores at a stable level and more effectively promoted gluconeogenesis when the dietary carbohydrates are absent to maintain glucose homoeostasis.During refeeding, as most teleostean, Siberian sturgeon failed controlling the activities and mRNA levels of phosphoenolpyruvate carboxykinase cytosolic forms(PEPCK-C), fructose-1,6-bisphosphatase(FBPase),but particularly controlled phosphoenolpyruvate carboxykinase mitochondrial forms(PEPCK-M) activities and mRNA expression of glucose-6-phosphatase(G6 Pase, except in GL group). Siberian sturgeon has a fu
基金Project supported by the Three Georges Construction Commission and the National Natural Science Foundation of China (Grant No. 30490231).
文摘The Chinese sturgeon （Acipenser Sinensis） is one of the unique and important fishery resources in China. Since the construction of the Gezhouba Dam, the traditional migration route of the sturgeon has been blocked; consequently, the length of natural spawning sites is reduced from 800 km in the past to less than 5 km at present. As an endangered species, the Chinese sturgeon has become one of the most conserved aquatic species. In this article, the flow field of its spawning states in the downstream of Gezhouba Dam was simulated and analyzed using N-S equations and k-ε turbulence model. Volume Of Fluid （VOF） method with the Finite Volume Method （FVM） was used to simulate the water-air two-phase flow to examine the computed area. On the basis of the ecological-hydraulic characteristics of Chinese sturgeon, the features of the flow field were investigated to provide theoretical support for the proper management of the Three Gorges Reservoir.
基金Project supported by the United States-China Natural Program.
文摘Gezhouba Dam was built in 1981 across the Yangtze River at Yichang, Hubei Prov-ince, China. It blocked the upstream spawning migration of all anadromous species offish, including the Chinese sturgeon Acipenser sinensis. Although the main historicalspawing areas of Chinese sturgeons were far upstream of the dam, successful spawning inthe reach below the dam has been documented each year since 1982 by collecting
文摘We conducted experiments in an artificial stream tank with wild juvenile Chinese sturgeon Acipenser sinensis captured in the Yangtze Estuary to test the null hypothesis that their feeding efficiency on Barcheek goby Rhinogobius giurinus was not affected by sand vs. smooth glass substrate. Gobies are among the most common prey eaten by wild juvenile A. sinensis in the estuary. Test results found neither substrate type significantly affected feeding efficiency by juveniles. Previous research found a strong innate habitat preference of A. sinensis for sand substrate. The present results indicate that the preference for sand is not related to efficiently capturing R. giurinus on sand, but is an adaptation predisposing juvenile A. sinensis to seek sandy substrate where R. giurinus and other benthic forage are the most abundant in the Yangtze Estuary.
文摘The paper presents the results of the sterlet sturgeon Acipenser ruthenus reintroduction into the Kama Reservoir(Perm Krai,Russia)by sterlet fry from hatcheries into the reservoir since 2001.The effectiveness of hatchery releases was evaluated by monitoring gillnet sampling 2014-2020.The results showed that the species was reported throughout entire Kama Reservoir,which contrasts with 1988-1994 data,when the species was registered only at the upper part of the reservoir near the Kama River mouth.The highest number and biomass values of catch per unit effort(CPUE)of the sterlet were found in submerged river channels-the deepest habitats with flow conditions which are close to the ecological requirements of the species.The distribution of the sterlet within the channel biotopes of the reservoir is defined by reduced variability.The biggest individuals and the widest size range of the sterlet were typical for the upper area of the reservoir and Chusovskoy Bay,which have the greatest flow.The smallest sizes were typical for fish from catches near the dam of the reservoir,which is consistent with the published data on the distribution of sterlet in reservoir conditions.The presented results may indicate the artificial formation of the sterlet stock in the Kama Reservoir maintained by hatchery releases.The revealed distribution of the sterlet in the reservoir determines the direction of further research on natural reproduction and thus formation of an artificially formed population.Also,future studies are required to assess the possibility of hybridization of the sterlet of artificial origin with natural populations of the species,since the low genetic diversity of the sterlet released into the Kama Reservoir which can lead to irreversible loss of genetic individuality of the natural stocks of the species in the catchment of upper course of the Kama River.
基金funded by the Special Fund for Agro-scientific Research in the Public Industry of China (201203085 )the Fundamental Research Fund of Central Welfare Scientific Research Institutes (201003)
文摘[Objective]This study was to investigate the effect of different concentrations of Norfloxacin on the superoxide dismutase （ SOD） activities in the blood plasma and liver tissues of Amur sturgeon （ Acipenser schrencki Brandt） and sterlet （ Acipenser ruthenus Linnaeus） . [Method] Using pharmaco-toxicological evaluation method,Norfloxacin with the concentrations of 0,20,40,60,80 and 100 mg /kg,was orally delivered to the Amur sturgeon and sterlet for 5 d,respectively. The SOD activities in the blood plasma and liver tissues were measured after drug withdrawal for 2 d to explore the optimal dosing concentration of Norfloxacin during sturgeon culture,as well as the effect of Norfloxacin on liver injury. [Result] SOD existed in both two sturgeons but with different amounts,and the SOD activities were higher in the livers than in the blood plasma no matter in the control and all drug delivered groups. Under different drug delivery concentrations,the SOD activities first increased and then decreased in the 2 tissues of the 2 sturgeon species,and the SOD activities reached the maximum when the drug delivery concentration was 40 mg /kg. The drug de- livery concentration had little effect on the SOD activities in the blood plasma,which showed stable changes. When the drug delivery concentration was 40 mg /kg,the SOD activities in the blood plasma of Amur sturgeon were higher than that of sterlet,while for the other concentrations,the SOD activities in the plasma of sterlet showed higher performance. However,the SOD activities changed significantly through drug delivery in the liver tis- sues,and the SOD activities were higher in the sterlet than in the Amur sturgeon when the drug delivery concentrations were 0,40 and 100 mg /kg. The SOD activity in the sterlet was the highest under 40 mg /kg,presenting a sharp peak value. The optimal drug delivery concentration of Norfloxa- cin was 30 -50 mg /kg,under which the Norfloxacin presented best effect and had no injury effect on livers. [Conclusion]This study provides
文摘Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></span><span style="font-family:Verdana;">) is a primitive freshwater fish and a source of black caviar.</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">The genes involved in sexual determination and differentiation are still unknown and there are no molecular markers for sex identification in this species. Studying the variation of the sex-based differences in genomic sequences and in gene expression in the sturgeon may lead to markers of sex in early stages of development and advances in aquaculture, as well as provide novel insights about the evolution of reproduction, sex determination, and sexual differentiation mechanisms in vertebrates. Previous studies by our and other groups have identified differentially expressed genes in the gonads of adult female and male sturgeon. The current study aimed to test whether these ge</span><span style="font-family:Verdana;">nes were also differentially expressed in non-gonadal tissue, namely fins. We measured by qRT-PCR the mRNA levels of 29 known and novel sex-related genes in the gonads and fins of males (4 years old) and females (7 years old;sexual maturation is earlier in males than in females). Six genes (ATP6, IGFRM, LIA1A, S1A, NPL1A, GAPDH and SOX9) showed higher expression in female fin</span><span style="font-family:Verdana;">s. However, only ATP6 mRNA levels differed in fins of males and females of the same age (4 years old). These findings underscore the impracticality of sex identification based on gene expression in non-gonadal</span><span style="font-family:Verdana;"> tissue and the need for genetic sex markers in the Russian sturgeon.
文摘The development of new agricultural industries is an important challenge for a region’s economic establishment. The connection between a research department at MIGAL Institute and biotechnology in aquaculture is an example (case study) of the interrelationships that also exist in other departments. This article describes the research conducted by the institute supporting the development of the aquaculture industry in Israel through the introduction of a relatively new fish species—the Russian sturgeon (Acipenser gueldenstaedtii), with the relevant information to be used for acclimatization to aquaculture conditions in northern Israel. Many aspects related to bringing a new species to Israel and working with this new industry to study its adaptation to existing conditions in the north of the country influenced the economic success of this new type of precision agriculture. This article describes the research on various aspects affecting the successful acclimatization of Russian sturgeon in northern Israel: introduction, food development, reproduction, hormone systems that control reproduction and growth, gonadal development, and molecular markers for sex determination, differentiation, and economic assessment of caviar production.
文摘[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.
文摘In the White sturgeon fish farms, some individuals have difficulty in getting access to food: such sturgeons are called "runts", and they result in a slower growth rate than normally feeding fish. In this paper, we have studied the gut peculiarities of runt sturgeons. Utilizing in paralleling an analysis of diatom populations in both the fish gut tissues and the rearing tank waters, we hypothesized a causative relation between the occurrence of runt sturgeons and periodic diatom blooms. In fact, we have observed that the diatom species identified in the aquatic environment were also detected in organs (Fragilaria spp and Rhoicosfenia spp for both glandular body, mid-intestine) of the runt sturgeon's gut, but not in tissues of normally feeding individuals. Owing to their siliceous wall, diatoms can be responsible for areas of epithetlial detachment in the mucosal surfaces of the alimentary canal and a catharral inflammation in both the gastric pits and intestinal folds which may be the cause of secondary bacterial diseases. We suggest that diatom blooms may contribute to the occurrence of runt sturgeons in the studied Italian fish farm.
基金supported by National Natural Science Foundation of China (31370047, U1301252)Shenzhen Scientific R & D Grant (GJHS20160331150703934)+1 种基金Shenzhen Dapeng Special Program for Industrial Development (KY20160102)Zhenjiang Leading Talent Program for Innovation and Entrepreneurship
文摘As a living fossil, the endangered Chinese sturgeon(Acipenser sinensis) has been considered a national treasure in China. Here,the famous Gezhouba Dam and Three Gorges Dam on the Yangtze River were built in 1988 and 2006, for economic purposes.The natural population of Chinese sturgeon has declined since then, as these dams block its migratory route to the original spawning grounds in the middle reaches of the Yangtze River. In 2013 and 2014, there was an absence of spawning where it typically happened near the Gezhouba Dam. Nevertheless, from April to June in 2015, over 1,000 larvae with different body lengths(10–35 cm) were detected along the Shanghai Yangtze Estuary; but only little is currently known about the population genetic structure of the Chinese sturgeon. Herein, we inferred population genetic parameters from 462 available Chinese sturgeon specimens based on a 421-bp fragment of the mitochondrial DNA(mtDNA) D-loop region and 1,481,620 SNPs(singlenucleotide polymorphisms) generated by restriction site-associated DNA sequencing(RAD-seq). For the D-loop dataset, 15 haplotypes were determined. Randomly picked 23 individuals, representing the 15 D-loop haplotype groups, were subsequently used for further RAD-seq validation. The average nucleotide diversity calculated from the mtDNA and RAD datasets was 0.0086 and 0.000478, respectively. The overall effective female population size was calculated to be 1,255 to 2,607, and the long-term effective population size was estimated to range from 11,950 to 119,500. We observed that the genetic variability and the effective female population size of the current population in the Yangtze River are severely low, which are similar to the data reported over 10 years ago. The deduced relatively small effective population of female fish, limiting the genetic connectivity among Chinese sturgeon, should be considered a serious threat to this endangered species.
文摘According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT-PCR, false sequencing was corrected, and full length eDNA of the ferritin subunit from the Chinese sturgeon was obtained. After being submitted to the GenBank database, the sequence accession number EU348782 was assigned. With the length of 896 bp, this eDNA includes entire coding regions of 53 lbp, which encodes 176 amino acids （aa）. The molecular weight was predicted to be 20339.9Mr and the theoretical isoelectric point 5.66. It shares 82.9% protein sequence homology with the ferritin of the Atlantic salmon. This gene is expressed in many organs of the Chinese sturgeon, for example, the liver, pancreas, muscle, brain, heart and gastric mucosa. The highest expression level was found in the pancreas and the heart, while the muscular tissue showed the lowest. Homology modeling was used to predict the 3-D structure of the protein, which included 5 alpha helices and 10 turns. The ferritin protein structure could be overlapped and showed high similarity with that of human, flog and bacteria. It was revealed that this kind of ferritin was highly conserved in structure and function.
基金Supported by the Project of Science and Technology Innovation(CXCZ2017331)Natural Science Foundation of Fujian Province(2021J01085 and 2020J01539)National Innovation and En-trepreneurship Program for College Students(202110389008)。
文摘[Objective] The paper was to understand the physiological, enzymatic and molecular changes of Acipenser schrencki induced by heat exposure. [Method] The effects of heat exposure on A. schrencki were investigated. The temperature was increased from(18± 0.5) ℃ by 0.08 ℃/min and respectively kept at(32±0.5) ℃ for 2, 4 and 6 h. The respiration rate, related biochemical indicators and heat shock protein 70(Hsp 70) mR-NA expression were investigated. [Result] At(32±0.5) ℃, the activities of total protein, albumin, globulin, urea nitrogen, ceatinine, malondialde-hyde, aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase fluctuated dramatically from 2 h to 6 h. Meanwhile, the respi-ration rate and Hsp 70 m RNA expression in heart, liver and spleen were significantly increased from 2 h to 6 h. In comparison, the lower respira-tion rate and tiny fluctuations in activities of biochemical indicators were consistent with the lower Hsp 70 expression. [Conclusion] During the process of heat stress, A. schrencki seems to be sensitive to such temperature, and manifests generally consistent gill breathing, biochemical indica-tors and Hsp70 gene expression, implying better heat tolerance.
