以丙酮为提取溶剂,采用微波辐射提取石榴皮中的单宁,考察了溶剂浓度、微波功率、微波辐射时间、液料比等因素对单宁提取量的影响。结果表明,当溶剂体积浓度50%、微波辐射功率320 W、微波辐射时间5 m in和液料比18∶1(g/g)时,单宁的提取...以丙酮为提取溶剂,采用微波辐射提取石榴皮中的单宁,考察了溶剂浓度、微波功率、微波辐射时间、液料比等因素对单宁提取量的影响。结果表明,当溶剂体积浓度50%、微波辐射功率320 W、微波辐射时间5 m in和液料比18∶1(g/g)时,单宁的提取量为268.54 mg/g,比普通水浴提取提高了30.39 mg/g。展开更多
研究锌离子络合法快速分离纯化荞麦壳黄酮工艺,并评价纯化组分对晚期糖基化终末产物(AGEs)生成的抑制活性。根据络合转化率和络合强度,将荞麦壳黄酮提取物(BHF)经过二次锌离子络合,分别获得荞麦壳黄酮中间提取物(IBHF)和荞麦壳高黄酮组...研究锌离子络合法快速分离纯化荞麦壳黄酮工艺,并评价纯化组分对晚期糖基化终末产物(AGEs)生成的抑制活性。根据络合转化率和络合强度,将荞麦壳黄酮提取物(BHF)经过二次锌离子络合,分别获得荞麦壳黄酮中间提取物(IBHF)和荞麦壳高黄酮组分(HBHF)。采用Box-Behnken响应曲面分析法优化获得IBHF组分的最佳工艺参数:乙酸锌与样品质量比2∶1,乙醇体积分数30%,氨水体积分数0.05%,室温反应10 min。在该条件下络合转化率为97.44%。IBHF组分经过二次锌离子络合,分离获得HBHF组分,其总黄酮含量为57.08 g RE/100 g(三氯化铝-乙酸盐比色法),其纯度比分离纯化前提高了6.64倍。高效液相色谱分析(HPLC)表明,HBHF组分含有异荭草素、牡荆素、异牡荆素、芦丁、山奈酚-3-O芸香糖苷5种主要黄酮,其中芦丁占比62.34%。HBHF组分表现出良好的抗AGEs活性,在质量浓度为200μg/mL时,Glu-BSA体系的中的AGEs抑制率为63.16%,MGO-BSA体系中的AGEs抑制率为42.98%,抑制率均显著优于阳性对照氨基胍。展开更多
Reactive oxygen species(ROS)-induced oxidative damage is strongly associated with the pathogenesis of chronic diseases,and natural antioxidant peptides have good abilities of scavenging ROS.The antioxidant activity of...Reactive oxygen species(ROS)-induced oxidative damage is strongly associated with the pathogenesis of chronic diseases,and natural antioxidant peptides have good abilities of scavenging ROS.The antioxidant activity of peptide Lys-Ser-Pro-Leu-Tyr(KSPLY)derived from Hericium erinaceus remains unclear.In the present study,the antioxidant effect and mechanism of KSPLY on H_(2)O_(2)-induced oxidative damage in HepG2 cells were investigated.The results indicated that KSPLY exhibited the antioxidant capacity in H_(2)O_(2)-induced HepG2 cells by enhancing superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and catalase(CAT)activities.In comparison with the H_(2)O_(2)-treated damage group,the apoptosis rate,ROS level,and malondialdehyde(MDA)content of HepG2 cells treated with KSPLY were significantly decreased.The H.erinaceus-derived peptide KSPLY pretreatment promoted the expression of detoxification and antioxidant enzymes via the Keap1/Nrf2 signal pathway,thereby inhibiting the generation of ROS and MDA.In conclusion,the H.erinaceus-derived peptide KSPLY effectively protected HepG2 cells against H_(2)O_(2)-induced oxidative damage,and it provided a theoretical basis for the further development of new natural antioxidants.展开更多
文摘研究锌离子络合法快速分离纯化荞麦壳黄酮工艺,并评价纯化组分对晚期糖基化终末产物(AGEs)生成的抑制活性。根据络合转化率和络合强度,将荞麦壳黄酮提取物(BHF)经过二次锌离子络合,分别获得荞麦壳黄酮中间提取物(IBHF)和荞麦壳高黄酮组分(HBHF)。采用Box-Behnken响应曲面分析法优化获得IBHF组分的最佳工艺参数:乙酸锌与样品质量比2∶1,乙醇体积分数30%,氨水体积分数0.05%,室温反应10 min。在该条件下络合转化率为97.44%。IBHF组分经过二次锌离子络合,分离获得HBHF组分,其总黄酮含量为57.08 g RE/100 g(三氯化铝-乙酸盐比色法),其纯度比分离纯化前提高了6.64倍。高效液相色谱分析(HPLC)表明,HBHF组分含有异荭草素、牡荆素、异牡荆素、芦丁、山奈酚-3-O芸香糖苷5种主要黄酮,其中芦丁占比62.34%。HBHF组分表现出良好的抗AGEs活性,在质量浓度为200μg/mL时,Glu-BSA体系的中的AGEs抑制率为63.16%,MGO-BSA体系中的AGEs抑制率为42.98%,抑制率均显著优于阳性对照氨基胍。
基金supported by the Natural Science Foundation of the Higher Education Institutions of Jiangsu Province(20KJB550016)the National Natural Science Foundation of China(32101944)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Reactive oxygen species(ROS)-induced oxidative damage is strongly associated with the pathogenesis of chronic diseases,and natural antioxidant peptides have good abilities of scavenging ROS.The antioxidant activity of peptide Lys-Ser-Pro-Leu-Tyr(KSPLY)derived from Hericium erinaceus remains unclear.In the present study,the antioxidant effect and mechanism of KSPLY on H_(2)O_(2)-induced oxidative damage in HepG2 cells were investigated.The results indicated that KSPLY exhibited the antioxidant capacity in H_(2)O_(2)-induced HepG2 cells by enhancing superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and catalase(CAT)activities.In comparison with the H_(2)O_(2)-treated damage group,the apoptosis rate,ROS level,and malondialdehyde(MDA)content of HepG2 cells treated with KSPLY were significantly decreased.The H.erinaceus-derived peptide KSPLY pretreatment promoted the expression of detoxification and antioxidant enzymes via the Keap1/Nrf2 signal pathway,thereby inhibiting the generation of ROS and MDA.In conclusion,the H.erinaceus-derived peptide KSPLY effectively protected HepG2 cells against H_(2)O_(2)-induced oxidative damage,and it provided a theoretical basis for the further development of new natural antioxidants.
