This paper deals with two chemical disinfectants on the growth and metabolism of Nannochloris oculata .The results show that at both the concentrations of availability Cl≥2.5mg/L and availability I≥0.2 mg/L and with...This paper deals with two chemical disinfectants on the growth and metabolism of Nannochloris oculata .The results show that at both the concentrations of availability Cl≥2.5mg/L and availability I≥0.2 mg/L and with the concentration of the chemical disinfectant, the growth rate, the Chlorophylla content, photosynthesis and respiration were reduced markedly. The higher the concentration is, the greater the toxicity will become. At the concentrations of availability Cl≥4.5 mg/L, Nannochloris oculata degrades markedly. Nannochloris oculata appears "toxicity excitement result"in the low levels Cl.展开更多
Amplified fragment length polymorphisms (AFLP) technique was used to analyze the fingerprint- ing of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed ...Amplified fragment length polymorphisms (AFLP) technique was used to analyze the fingerprint- ing of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to fur- ther characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a dis- ease-resistance trait but were not functional genes.展开更多
据对虾白斑综合症病毒(WSSV)的基因保守序列,使用Primer Explorer V3软件设计了2条引物,利用PCR的扩增产物,结合变性高效液相色谱(DHPLC)技术,建立了一种对虾白斑综合症病毒的DHPLC快速检测方法。该检测方法特异性好,与传染性皮下和造...据对虾白斑综合症病毒(WSSV)的基因保守序列,使用Primer Explorer V3软件设计了2条引物,利用PCR的扩增产物,结合变性高效液相色谱(DHPLC)技术,建立了一种对虾白斑综合症病毒的DHPLC快速检测方法。该检测方法特异性好,与传染性皮下和造血器官坏死病毒、斑节对虾杆状病毒、肝胰腺细小病毒、对虾杆状病毒以及对虾基因组DNA无交叉反应;检测灵敏度较高,约为10-5ng/μL,高于常规PCR及荧光定量PCR的灵敏度。用建立的DHPLC方法对100尾对虾样品进行了临床检测,结果与荧光定量PCR检测结果一致。WSSV的DHPLC检测方法,特异性强、灵敏度高,是对WSSV进行有效检测的一种新方法。展开更多
文摘This paper deals with two chemical disinfectants on the growth and metabolism of Nannochloris oculata .The results show that at both the concentrations of availability Cl≥2.5mg/L and availability I≥0.2 mg/L and with the concentration of the chemical disinfectant, the growth rate, the Chlorophylla content, photosynthesis and respiration were reduced markedly. The higher the concentration is, the greater the toxicity will become. At the concentrations of availability Cl≥4.5 mg/L, Nannochloris oculata degrades markedly. Nannochloris oculata appears "toxicity excitement result"in the low levels Cl.
基金This research was supported by special funds from the National KeyBasic Research Program (G1999012007) and the National High-TechResearch and Development Program of China (863 Program,2001AA620105)
文摘Amplified fragment length polymorphisms (AFLP) technique was used to analyze the fingerprint- ing of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to fur- ther characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a dis- ease-resistance trait but were not functional genes.
文摘据对虾白斑综合症病毒(WSSV)的基因保守序列,使用Primer Explorer V3软件设计了2条引物,利用PCR的扩增产物,结合变性高效液相色谱(DHPLC)技术,建立了一种对虾白斑综合症病毒的DHPLC快速检测方法。该检测方法特异性好,与传染性皮下和造血器官坏死病毒、斑节对虾杆状病毒、肝胰腺细小病毒、对虾杆状病毒以及对虾基因组DNA无交叉反应;检测灵敏度较高,约为10-5ng/μL,高于常规PCR及荧光定量PCR的灵敏度。用建立的DHPLC方法对100尾对虾样品进行了临床检测,结果与荧光定量PCR检测结果一致。WSSV的DHPLC检测方法,特异性强、灵敏度高,是对WSSV进行有效检测的一种新方法。
