Background：Increasing evidence supports an association between periodontitis and systemic diseases.Leptin is involved both in the energy metabolism and inflammatory processes and is suggested to be a link between per...Background：Increasing evidence supports an association between periodontitis and systemic diseases.Leptin is involved both in the energy metabolism and inflammatory processes and is suggested to be a link between periodontal infection and systemic health.The present study aimed to evaluate the peripheral leptin concentration in patients with aggressive periodontitis （AgP） and to explore the relationship between leptin and systemic inflammation.Methods：Ninety patients with AgP visiting the Clinic of the Periodontology Department,Peking University School and Hospital of Stomatology between July 2001 and May 2006,and 44 healthy controls （staffand student volunteers in the same institute） were recruited.Plasma levels of leptin and inflammatory cytokines including interleukin （IL）-1β,I L-6,tumor necrosis factor-α （TNF-α） and C-reactive protein （CRP） were measured by enzyme-linked immunosorbent assay.Correlation and multiple linear regression analysis were performed to analyze the association between plasma leptin level and other variables.Results：Plasma leptin level of AgP group was significantly higher than that of the control group （19.7-4.4 ng/ml vs.7.5 ± 1.3 ng/ml,P ＜ 0.01）.After controlling for age,gender,and body mass index,positive correlation was observed between plasma leptin concentration and log-transformed levels of pro-inflammatory cytokines （IL-1β,IL-6,TNF-α and CRP）,and the partial correlation coefficients ranged from 0.199 to 0.376 （P ＜ 0.05）.Log-transformed IL-1β and I L-6 levels entered the final regression model （standardized β were 0.422 and 0.461 respectively,P ＜ 0.01）.Conclusions：Elevated plasma leptin concentration may be associated with increased systemic levels of inflammatory markers in AgP patients.展开更多
为探索金属离子螯合剂乙二胺四乙酸(EDTA)单独及联合抗菌药物对水霉菌生物膜的清除效应,采用微量稀释法测定EDTA对水霉菌游动孢子最小抑菌浓度(MIC)。用常用剂量和2倍剂量抗菌药亚甲基蓝、美婷及1、4、16倍MIC的EDTA联合二者常用剂量分...为探索金属离子螯合剂乙二胺四乙酸(EDTA)单独及联合抗菌药物对水霉菌生物膜的清除效应,采用微量稀释法测定EDTA对水霉菌游动孢子最小抑菌浓度(MIC)。用常用剂量和2倍剂量抗菌药亚甲基蓝、美婷及1、4、16倍MIC的EDTA联合二者常用剂量分别作用于水霉菌生物膜,CCK-8法检测药物作用后生物膜活力变化。并用Calcofluor White M2R染色EDTA处理后水霉菌生物膜,荧光显微镜观察胞外基质变化。结果显示,亚甲基蓝、美婷在常用剂量下作用于水霉菌生物膜效果不佳,与对照组无显著性差异(P>0.05),亚甲基蓝在2倍剂量下仍不能发挥较好的效果,而亚甲基蓝、美婷与EDTA联合作用均表现出较好的作用。EDTA作用后水霉菌生物膜结构破坏,胞外基质减少。研究结果表明,EDTA可破坏水霉菌生物膜结构,与抗菌药联合使用对生物膜有显著的清除作用。展开更多
基金grants from National Nature Science Foundation of China，key program of clinical specialty,National Ministry of Health
文摘Background：Increasing evidence supports an association between periodontitis and systemic diseases.Leptin is involved both in the energy metabolism and inflammatory processes and is suggested to be a link between periodontal infection and systemic health.The present study aimed to evaluate the peripheral leptin concentration in patients with aggressive periodontitis （AgP） and to explore the relationship between leptin and systemic inflammation.Methods：Ninety patients with AgP visiting the Clinic of the Periodontology Department,Peking University School and Hospital of Stomatology between July 2001 and May 2006,and 44 healthy controls （staffand student volunteers in the same institute） were recruited.Plasma levels of leptin and inflammatory cytokines including interleukin （IL）-1β,I L-6,tumor necrosis factor-α （TNF-α） and C-reactive protein （CRP） were measured by enzyme-linked immunosorbent assay.Correlation and multiple linear regression analysis were performed to analyze the association between plasma leptin level and other variables.Results：Plasma leptin level of AgP group was significantly higher than that of the control group （19.7-4.4 ng/ml vs.7.5 ± 1.3 ng/ml,P ＜ 0.01）.After controlling for age,gender,and body mass index,positive correlation was observed between plasma leptin concentration and log-transformed levels of pro-inflammatory cytokines （IL-1β,IL-6,TNF-α and CRP）,and the partial correlation coefficients ranged from 0.199 to 0.376 （P ＜ 0.05）.Log-transformed IL-1β and I L-6 levels entered the final regression model （standardized β were 0.422 and 0.461 respectively,P ＜ 0.01）.Conclusions：Elevated plasma leptin concentration may be associated with increased systemic levels of inflammatory markers in AgP patients.
文摘为探索金属离子螯合剂乙二胺四乙酸(EDTA)单独及联合抗菌药物对水霉菌生物膜的清除效应,采用微量稀释法测定EDTA对水霉菌游动孢子最小抑菌浓度(MIC)。用常用剂量和2倍剂量抗菌药亚甲基蓝、美婷及1、4、16倍MIC的EDTA联合二者常用剂量分别作用于水霉菌生物膜,CCK-8法检测药物作用后生物膜活力变化。并用Calcofluor White M2R染色EDTA处理后水霉菌生物膜,荧光显微镜观察胞外基质变化。结果显示,亚甲基蓝、美婷在常用剂量下作用于水霉菌生物膜效果不佳,与对照组无显著性差异(P>0.05),亚甲基蓝在2倍剂量下仍不能发挥较好的效果,而亚甲基蓝、美婷与EDTA联合作用均表现出较好的作用。EDTA作用后水霉菌生物膜结构破坏,胞外基质减少。研究结果表明,EDTA可破坏水霉菌生物膜结构,与抗菌药联合使用对生物膜有显著的清除作用。
