Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile（Pt CAT） was clo...Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile（Pt CAT） was cloned using RTPCR and rapid amplification of c DNA ends（RACE）. Pt CAT is 1 921 bp long and consists of a 5′-UTR of 50 bp, a 3′-UTR of 349 bp, and an ORF of 1 542 bp that encodes 513 amino acids with a molecular weight of 58.4 k D and an estimated isoelectric point of 8.2. Sequence alignment indicated that Pt CAT contained a highly conserved catalytic signature motif（^（61）FNRERIPERVVHAKGAG^（77））, a proximal heme-ligand signature sequence（^（352）RLFSYSDP^（359））, and three catalytic amino acid residues（H^（72）, N^（145）, and Y^（356））. Pt CAT also contains two putative N-glycosylation sites（^（34）NKT^（36） and ^（437）NFT^（439）） and a peroxisome-targeting signal（^（511）AQL^（513））. Furthermore, Pt CAT shares 53%–88% identity and 29%–89% similarity with other catalase amino acid sequences. Pt CAT m RNA was present in all tested organs, including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, but its expression was highest in the digestive gland. High-temperature-induced stress produced two expression patterns of Pt CAT m RNA： first, an initial up-regulation followed by a down-regulation in the heart, digestive gland, and gonad and, second, consistent down-regulation in all other organs. These results demonstrate that Pt CAT is a typical member of the catalase family and might be involved in the responses to harmful environmental factors.展开更多
【目的】近年来,红螯螯虾养殖面积越来越广泛,明确不同规格的红螯螯虾对氨氮和亚硝酸盐的耐受力,有利于提高其养成率,促进其养殖业的健康发展。【方法】在水温24~25℃、p H 7.9~8.0的条件下,研究了氨氮和亚硝酸盐对红螯螯虾幼虾和亚成...【目的】近年来,红螯螯虾养殖面积越来越广泛,明确不同规格的红螯螯虾对氨氮和亚硝酸盐的耐受力,有利于提高其养成率,促进其养殖业的健康发展。【方法】在水温24~25℃、p H 7.9~8.0的条件下,研究了氨氮和亚硝酸盐对红螯螯虾幼虾和亚成虾的急性毒性,分析半致死浓度(LC50)和安全浓度(SC)。【结果】总氨氮对红螯螯虾幼虾的24、48、72和96 h LC50分别为188.0、136.15、104.67和88.00 mg·L^(-1),SC为8.80 mg·L^(-1);总氨氮对亚成虾的24、48、72和96 h LC50分别为344.01、270.46、205.15和167.68 mg·L^(-1),SC为16.77 mg·L^(-1);非离子氨对幼虾的24、48、72和96 h LC50分别为10.16、7.35、5.65和4.75 mg·L^(-1),SC为0.48 mg·L^(-1);非离子氨对亚成虾的24、48、72和96 h LC50分别为18.58、14.60、11.08和9.05 mg·L^(-1),SC为0.91 mg·L^(-1);亚硝酸盐对幼虾的24、48、72和96 h LC50分别为46.76、33.88、27.97和22.81 mg·L^(-1),SC为2.28 mg·L^(-1);亚硝酸盐对亚成虾的24、48、72和96 h LC50分别为77.56、59.33、45.41和37.48 mg·L^(-1),SC为3.75 mg·L^(-1)。【结论】红螯螯虾对氨氮的耐受力高于亚硝酸盐,亚成虾对氨氮和亚硝酸盐的耐受力高于幼虾。展开更多
通过对福建牡蛎幼体不同发育时期的转录组文库比对分析,结果显示牡蛎附着变态相关蛋白1(settlement and metamorphosis related protein1,SMRP1)基因在幼体附着变态时期高表达,利用定量即时聚合酶连锁反应(quantitative real time polym...通过对福建牡蛎幼体不同发育时期的转录组文库比对分析,结果显示牡蛎附着变态相关蛋白1(settlement and metamorphosis related protein1,SMRP1)基因在幼体附着变态时期高表达,利用定量即时聚合酶连锁反应(quantitative real time polymerase chain reaction,qRT-PCR)技术确认了SMRP1基因在幼体附着变态时期高表达,且进一步发现SMRP1集中表达在牡蛎鳃和外套膜组织中,具有明显的组织特异性。利用组织和整体原位杂交技术检测SMRP1基因在不同时期幼体以及成体的外套膜和鳃组织中的表达定位情况,发现SMRP1基因集中表达在幼体壳顶时期到眼点幼虫的时期,在成体鳃和外套膜组织集中表达在组织的外层上皮细胞中。结合牡蛎幼体在附着变态时期鳃丝的快速生长发育及其在成体外套膜中的功能作用分析表明SMRP1基因对牡蛎幼体鳃和外套膜的形成和生长发育具有重要的作用。该研究为解析胰岛素相关多肽受体信号通路的功能提供数据支持。展开更多
The present study was to investigate the copepodid species infecting rosy goatfish Parupeneus rubescens,one of the most economically important fishes in the Arabian Gulf.A copepodid species identified from the examine...The present study was to investigate the copepodid species infecting rosy goatfish Parupeneus rubescens,one of the most economically important fishes in the Arabian Gulf.A copepodid species identified from the examined fish specimens belongs to the Taeniacanthidae family and is labeled as Taeniacanthus yamagutii Shiino,1957,mainly depending on its morphological,morphometric,and ultrastructural characteristics,in particular the presence of maxilliped claw with a conspicuous digitiform process at the base,the terminal process of the second maxilla stout,and a setiform element at the tip of each exopod spine of legs 2-4.In order to ensure the accurate identification and exact taxonomic characterization of this species,the 28S rRNA gene sequence was analyzed.The result revealed that the present copepodid species belong to the Taenicanthidae family and has a close relationship with Taeniacanthus yamagutii(gb|KR048852.1)in the same taxon.The present study demonstrated that the rosy goatfish is a host for Taeniacanthus species,which will be helpful to prevent this parasitic infection.展开更多
Long non-coding RNAs(lncRNAs)function as key modulators in mammalian immunity,particularly due to their involvement in lncRNA-mediated competitive endogenous RNA(ceRNA)crosstalk.Despite their recognized significance i...Long non-coding RNAs(lncRNAs)function as key modulators in mammalian immunity,particularly due to their involvement in lncRNA-mediated competitive endogenous RNA(ceRNA)crosstalk.Despite their recognized significance in mammals,research on lncRNAs in lower vertebrates remains limited.In the present study,we characterized the first immune-related lncRNA(pol-lnc78)in the teleost Japanese flounder(Paralichthys olivaceus).Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein(SARM),the fifth discovered member of the Toll/interleukin 1(IL-1)receptor(TIR)adaptor family.This ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor(TLR)signaling pathway.Specifically,SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1βand tumor necrosis factor-α(TNF-α).Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3’untranslated region(UTR),thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial dissemination.Furthermore,pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM expression.During infection,the negative regulators pol-lnc78 and SARM were significantly down-regulated,while pol-miR-n199-3p was significantly up-regulated,thus favoring host antibacterial defense.These findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.展开更多
在水产养殖中,周期性高温(Periodic high temperature,PHT)对虾类的生物学功能会产生显著影响。鳃作为虾类的呼吸和代谢器官,在维持体内渗透平衡方面具有关键作用,但其功能易受高温等环境变化的干扰。为了探究有效的对虾PHT应对策略,研...在水产养殖中,周期性高温(Periodic high temperature,PHT)对虾类的生物学功能会产生显著影响。鳃作为虾类的呼吸和代谢器官,在维持体内渗透平衡方面具有关键作用,但其功能易受高温等环境变化的干扰。为了探究有效的对虾PHT应对策略,研究了三丁酸甘油酯(Tributyrin,TB)对PHT[(34±1)℃,7 d]胁迫下凡纳滨对虾(Litopenaeus van-namei)鳃组织形态和生理功能的影响。结果表明,PHT胁迫导致了显著的鳃组织损伤,诱导了氧化应激反应,进而造成内质网应激、细胞凋亡以及渗透调节功能障碍。不同饲喂策略下(胁迫前饲喂三丁酸甘油酯7 d;每天饲喂三丁酸甘油酯;胁迫后饲喂三丁酸甘油酯7 d),鳃组织损伤程度均显著减轻,同时氧化应激水平降低。此外,三丁酸甘油酯还正向调节了PHT诱导的氧化应激、内质网应激、细胞凋亡以及渗透调节基因的相对表达水平。尤其是每天饲喂三丁酸甘油酯能够有效保持鳃组织形态完整和生理功能稳定。展开更多
[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head...[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head kidney cells. [Re- sult] The diploid chromosome number （2n） of Chi-lin fish was 50, and the karyotype of V. macrolepis was 2n=50,16m＋14sm＋20t, NF =80. No sex-chromosome was found in Chi-lin fish. [Conclusion] A detailed karyotype of this endemic cyprinid fish of Taishan Mountain was established for the first time in this study.展开更多
In this study,we investigated the community structure of crustaceans(decapod and stomatopod) inhabiting the sandy mud bottoms of Laizhou Bay(northeastern China) monthly from May 2011 to April 2012.Investigation was st...In this study,we investigated the community structure of crustaceans(decapod and stomatopod) inhabiting the sandy mud bottoms of Laizhou Bay(northeastern China) monthly from May 2011 to April 2012.Investigation was stopped from December 2011 to February 2012 because of the extreme weather and sea ice.A total of 205,057 specimens belonging to 31 species(shrimp,15;crab,15;and stomatopod,1) were collected in 148 hauls.From 2011 to 2012,Oratosquilla oratoria was the dominant biomass species(47.80%),followed by Charybdis japonica(15.49%),Alpheus japonicas(12.61%),Portunus trituberculatus(6.46%),and Crangon spp.(4.19%).Crangon spp.was the most dominant species by individual(32.55%).O.oratoria was the most-frequently encountered species(81.76%),followed by Palaemon gravieri(70.95%),C.japonica(65.54%),A.japonicas(62.16%),and P.trituberculatus(54.73%).The biomass density increased from August to September 2011 and decreased from March 2012 to April 2012.The dynamics of the ecological indices evolve in a similar manner,with high values of diversity and evenness and rich species from May to June 2011 and low values from September to October 2011.O.oratoria,C.japonica,and P.trituberculatus differed by biomass data between groups I(samples obtained from September to October 2011) and II(samples in other months).These species contributed more than 70% to the similarity of the crustacean community structure.Furthermore,the subsets of environmental variables that best matched the crustacean-assemblage structure were as follows:water depth(WD) in summer(June to August);sea surface temperature(SST),dissolved oxygen(DO),and WD in autumn(September to November);and DO,salinity,and WD in spring(March to May).The calculated correlation coefficients and significance level were higher in the period of July to August 2011 than in other months.Comparing 2011 to 2012 with 1982 to 1983,the species composition remained stable.However,the dominant species changed significantly.High value and large species,such as F.chinensis,P.trituberculatus,展开更多
基金The National Natural Science Foundation of China under contract No.31172397the New Century Excellent Talents of Fujian Province University under contract No.JA14167the Open Research Fund Program of Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment under contract No.Z814041
文摘Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile（Pt CAT） was cloned using RTPCR and rapid amplification of c DNA ends（RACE）. Pt CAT is 1 921 bp long and consists of a 5′-UTR of 50 bp, a 3′-UTR of 349 bp, and an ORF of 1 542 bp that encodes 513 amino acids with a molecular weight of 58.4 k D and an estimated isoelectric point of 8.2. Sequence alignment indicated that Pt CAT contained a highly conserved catalytic signature motif（^（61）FNRERIPERVVHAKGAG^（77））, a proximal heme-ligand signature sequence（^（352）RLFSYSDP^（359））, and three catalytic amino acid residues（H^（72）, N^（145）, and Y^（356））. Pt CAT also contains two putative N-glycosylation sites（^（34）NKT^（36） and ^（437）NFT^（439）） and a peroxisome-targeting signal（^（511）AQL^（513））. Furthermore, Pt CAT shares 53%–88% identity and 29%–89% similarity with other catalase amino acid sequences. Pt CAT m RNA was present in all tested organs, including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, but its expression was highest in the digestive gland. High-temperature-induced stress produced two expression patterns of Pt CAT m RNA： first, an initial up-regulation followed by a down-regulation in the heart, digestive gland, and gonad and, second, consistent down-regulation in all other organs. These results demonstrate that Pt CAT is a typical member of the catalase family and might be involved in the responses to harmful environmental factors.
文摘【目的】近年来,红螯螯虾养殖面积越来越广泛,明确不同规格的红螯螯虾对氨氮和亚硝酸盐的耐受力,有利于提高其养成率,促进其养殖业的健康发展。【方法】在水温24~25℃、p H 7.9~8.0的条件下,研究了氨氮和亚硝酸盐对红螯螯虾幼虾和亚成虾的急性毒性,分析半致死浓度(LC50)和安全浓度(SC)。【结果】总氨氮对红螯螯虾幼虾的24、48、72和96 h LC50分别为188.0、136.15、104.67和88.00 mg·L^(-1),SC为8.80 mg·L^(-1);总氨氮对亚成虾的24、48、72和96 h LC50分别为344.01、270.46、205.15和167.68 mg·L^(-1),SC为16.77 mg·L^(-1);非离子氨对幼虾的24、48、72和96 h LC50分别为10.16、7.35、5.65和4.75 mg·L^(-1),SC为0.48 mg·L^(-1);非离子氨对亚成虾的24、48、72和96 h LC50分别为18.58、14.60、11.08和9.05 mg·L^(-1),SC为0.91 mg·L^(-1);亚硝酸盐对幼虾的24、48、72和96 h LC50分别为46.76、33.88、27.97和22.81 mg·L^(-1),SC为2.28 mg·L^(-1);亚硝酸盐对亚成虾的24、48、72和96 h LC50分别为77.56、59.33、45.41和37.48 mg·L^(-1),SC为3.75 mg·L^(-1)。【结论】红螯螯虾对氨氮的耐受力高于亚硝酸盐,亚成虾对氨氮和亚硝酸盐的耐受力高于幼虾。
文摘通过对福建牡蛎幼体不同发育时期的转录组文库比对分析,结果显示牡蛎附着变态相关蛋白1(settlement and metamorphosis related protein1,SMRP1)基因在幼体附着变态时期高表达,利用定量即时聚合酶连锁反应(quantitative real time polymerase chain reaction,qRT-PCR)技术确认了SMRP1基因在幼体附着变态时期高表达,且进一步发现SMRP1集中表达在牡蛎鳃和外套膜组织中,具有明显的组织特异性。利用组织和整体原位杂交技术检测SMRP1基因在不同时期幼体以及成体的外套膜和鳃组织中的表达定位情况,发现SMRP1基因集中表达在幼体壳顶时期到眼点幼虫的时期,在成体鳃和外套膜组织集中表达在组织的外层上皮细胞中。结合牡蛎幼体在附着变态时期鳃丝的快速生长发育及其在成体外套膜中的功能作用分析表明SMRP1基因对牡蛎幼体鳃和外套膜的形成和生长发育具有重要的作用。该研究为解析胰岛素相关多肽受体信号通路的功能提供数据支持。
基金This study was supported by Researchers Supporting Project(No.RSP-2020/25),King Saud University,Riyadh,Saudi Arabia.
文摘The present study was to investigate the copepodid species infecting rosy goatfish Parupeneus rubescens,one of the most economically important fishes in the Arabian Gulf.A copepodid species identified from the examined fish specimens belongs to the Taeniacanthidae family and is labeled as Taeniacanthus yamagutii Shiino,1957,mainly depending on its morphological,morphometric,and ultrastructural characteristics,in particular the presence of maxilliped claw with a conspicuous digitiform process at the base,the terminal process of the second maxilla stout,and a setiform element at the tip of each exopod spine of legs 2-4.In order to ensure the accurate identification and exact taxonomic characterization of this species,the 28S rRNA gene sequence was analyzed.The result revealed that the present copepodid species belong to the Taenicanthidae family and has a close relationship with Taeniacanthus yamagutii(gb|KR048852.1)in the same taxon.The present study demonstrated that the rosy goatfish is a host for Taeniacanthus species,which will be helpful to prevent this parasitic infection.
基金supported by the National Natural Science Foundation of China(42006082)Natural Science Foundation of Jiangsu Province of China(BK20221323)+1 种基金“JBGS”Project of Seed Industry Revitalization in Jiangsu Province(JBGS[2021]034)State Key Laboratory of Developmental Biology of Freshwater Fish(2021KF009)。
文摘Long non-coding RNAs(lncRNAs)function as key modulators in mammalian immunity,particularly due to their involvement in lncRNA-mediated competitive endogenous RNA(ceRNA)crosstalk.Despite their recognized significance in mammals,research on lncRNAs in lower vertebrates remains limited.In the present study,we characterized the first immune-related lncRNA(pol-lnc78)in the teleost Japanese flounder(Paralichthys olivaceus).Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein(SARM),the fifth discovered member of the Toll/interleukin 1(IL-1)receptor(TIR)adaptor family.This ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor(TLR)signaling pathway.Specifically,SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1βand tumor necrosis factor-α(TNF-α).Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3’untranslated region(UTR),thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial dissemination.Furthermore,pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM expression.During infection,the negative regulators pol-lnc78 and SARM were significantly down-regulated,while pol-miR-n199-3p was significantly up-regulated,thus favoring host antibacterial defense.These findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.
基金Supported by National Natural Science Foundation of China (31172074,30700071)Natural Science Foundation of Shandong Province (ZR2010CL002)~~
文摘[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head kidney cells. [Re- sult] The diploid chromosome number （2n） of Chi-lin fish was 50, and the karyotype of V. macrolepis was 2n=50,16m＋14sm＋20t, NF =80. No sex-chromosome was found in Chi-lin fish. [Conclusion] A detailed karyotype of this endemic cyprinid fish of Taishan Mountain was established for the first time in this study.
基金supported by the National Basic Research Program of China (973 Program) (No.2015CB453303)the Special Fund for Agro-scientific Research in the Public Interest (No.201303050)the Taishan Scholar Program of Shandong Province (200867)
文摘In this study,we investigated the community structure of crustaceans(decapod and stomatopod) inhabiting the sandy mud bottoms of Laizhou Bay(northeastern China) monthly from May 2011 to April 2012.Investigation was stopped from December 2011 to February 2012 because of the extreme weather and sea ice.A total of 205,057 specimens belonging to 31 species(shrimp,15;crab,15;and stomatopod,1) were collected in 148 hauls.From 2011 to 2012,Oratosquilla oratoria was the dominant biomass species(47.80%),followed by Charybdis japonica(15.49%),Alpheus japonicas(12.61%),Portunus trituberculatus(6.46%),and Crangon spp.(4.19%).Crangon spp.was the most dominant species by individual(32.55%).O.oratoria was the most-frequently encountered species(81.76%),followed by Palaemon gravieri(70.95%),C.japonica(65.54%),A.japonicas(62.16%),and P.trituberculatus(54.73%).The biomass density increased from August to September 2011 and decreased from March 2012 to April 2012.The dynamics of the ecological indices evolve in a similar manner,with high values of diversity and evenness and rich species from May to June 2011 and low values from September to October 2011.O.oratoria,C.japonica,and P.trituberculatus differed by biomass data between groups I(samples obtained from September to October 2011) and II(samples in other months).These species contributed more than 70% to the similarity of the crustacean community structure.Furthermore,the subsets of environmental variables that best matched the crustacean-assemblage structure were as follows:water depth(WD) in summer(June to August);sea surface temperature(SST),dissolved oxygen(DO),and WD in autumn(September to November);and DO,salinity,and WD in spring(March to May).The calculated correlation coefficients and significance level were higher in the period of July to August 2011 than in other months.Comparing 2011 to 2012 with 1982 to 1983,the species composition remained stable.However,the dominant species changed significantly.High value and large species,such as F.chinensis,P.trituberculatus,
