The effects of different treatments on cross compatibility in distant hybridization werestudied in 69 cross combinations among 9 cultivars of stone fruits. The results showedthat the setting percentage in balloon stag...The effects of different treatments on cross compatibility in distant hybridization werestudied in 69 cross combinations among 9 cultivars of stone fruits. The results showedthat the setting percentage in balloon stage was obviously higher than that in earlyblooming stage. The differences of fruit set between positive and reverse crosses weresignificant. Female parent affected cross compatibility more remarkably than male parentin distant hybridization. The appropriate electrostatic field, He-Ne laser combinedtreatment with 60Co γ-ray and He-Ne laser could improve the pollen germination rate invitro effectively, the fruit set percentage was higher than control, while 60Co γ-raytreatment reduced the pollen germination rate in vitro, and the fruit set percentageobtained by distant hybridization was lower than control.展开更多
Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from th...Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.展开更多
文摘The effects of different treatments on cross compatibility in distant hybridization werestudied in 69 cross combinations among 9 cultivars of stone fruits. The results showedthat the setting percentage in balloon stage was obviously higher than that in earlyblooming stage. The differences of fruit set between positive and reverse crosses weresignificant. Female parent affected cross compatibility more remarkably than male parentin distant hybridization. The appropriate electrostatic field, He-Ne laser combinedtreatment with 60Co γ-ray and He-Ne laser could improve the pollen germination rate invitro effectively, the fruit set percentage was higher than control, while 60Co γ-raytreatment reduced the pollen germination rate in vitro, and the fruit set percentageobtained by distant hybridization was lower than control.
基金supported by the Technological Production Transformation Foundation by the Ministry of Science and Technology of China(2002370010495)the foundation of Shandong Fruit Tree Three-Zero Project
文摘Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.
