2011年采用大田药效试验的方法,研究60％丁草胺·噁草酮乳油防除水稻旱直播田杂草的防除效果、最佳使用剂量及安全性.该药剂在水稻旱直播田播后苗前土壤喷雾处理,推荐使用药量为720～900 g a.i./hm2,可有效地防除稗草、千金子等禾...2011年采用大田药效试验的方法,研究60％丁草胺·噁草酮乳油防除水稻旱直播田杂草的防除效果、最佳使用剂量及安全性.该药剂在水稻旱直播田播后苗前土壤喷雾处理,推荐使用药量为720～900 g a.i./hm2,可有效地防除稗草、千金子等禾本科杂草及碎米莎草、水虱草等莎草科杂草.在推荐使用剂量内对水稻安全,高浓度处理会产生部分药害,后期可以恢复.展开更多
Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional g...Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional gel electrophoresis （2-DE） and mass spectrometry were used to separate and identify differentially expressed salivary gland proteins between the fed and unfed female R. haemaphysaloides. The soluble proteins from the salivary glands of fed and unfed female R. haemaphysaloides were separated by a sequential extraction method followed by 2-DE and 2-DE images. Image analysis of the gels revealed 1 096 ± 87 protein spots from the fed female ticks and 991 ±64 protein spots from the unfed female ticks. Among those protein spots, about 724 ±34 were present both in the fed and unfed female ticks. Fourteen spots from the fed ticks and six spots from the unfed ticks were selected for peptide mass fingerprinting （PMF） and sequencing assay by mass spectrometry （MS）. Bioinformatic analysis showed that a majority of the differentially expressed proteins were involved in signal transduction, metabolism, and transcriptional regulation. These differentially expressed proteins might be antigen candidates for the development of vaccines against the tick.展开更多
文摘2011年采用大田药效试验的方法,研究60％丁草胺·噁草酮乳油防除水稻旱直播田杂草的防除效果、最佳使用剂量及安全性.该药剂在水稻旱直播田播后苗前土壤喷雾处理,推荐使用药量为720～900 g a.i./hm2,可有效地防除稗草、千金子等禾本科杂草及碎米莎草、水虱草等莎草科杂草.在推荐使用剂量内对水稻安全,高浓度处理会产生部分药害,后期可以恢复.
基金supported by the National High Technology Research and Development Program of China(2006AA 10A207)the Basic Research Foundationfor National Commonweal Institute of China(2006JB02)
文摘Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional gel electrophoresis （2-DE） and mass spectrometry were used to separate and identify differentially expressed salivary gland proteins between the fed and unfed female R. haemaphysaloides. The soluble proteins from the salivary glands of fed and unfed female R. haemaphysaloides were separated by a sequential extraction method followed by 2-DE and 2-DE images. Image analysis of the gels revealed 1 096 ± 87 protein spots from the fed female ticks and 991 ±64 protein spots from the unfed female ticks. Among those protein spots, about 724 ±34 were present both in the fed and unfed female ticks. Fourteen spots from the fed ticks and six spots from the unfed ticks were selected for peptide mass fingerprinting （PMF） and sequencing assay by mass spectrometry （MS）. Bioinformatic analysis showed that a majority of the differentially expressed proteins were involved in signal transduction, metabolism, and transcriptional regulation. These differentially expressed proteins might be antigen candidates for the development of vaccines against the tick.
