[Objectives]This study was conducted to clarify the species,occurrence and distribution characteristics of sugarcane rust pathogens in low-latitude plateau sugarcane rust areas of Yunnan Province and the phylogenetic ...[Objectives]This study was conducted to clarify the species,occurrence and distribution characteristics of sugarcane rust pathogens in low-latitude plateau sugarcane rust areas of Yunnan Province and the phylogenetic relationship between the pathogens and that between them and other rust fungi of the genus Puccinia.[Methods]Fifty seven sugarcane rust samples collected from the sugarcane areas of Baoshan,Lincang,Menghai,Menglian,Ximeng,Lancang and Wenshan in Yunnan were subjected to molecular identification.[Results]The four sugarcane rust samples from Haiyin1 hao in Menghai,Yunnan Province belonged to orange rust of sugarcane,and the pathogen was Puccinia kuehnii Butler.;and other 53 samples of sugarcane rust belonged to brown rust of sugarcane,and the pathogen was Puccinia melanocephala Syd.et P.Syd.The rDNA sequences of other rust fungi from the genus Puccinia were downloaded from GenBank and used for the construction of an NJ tree for systematic evolution analysis together with the sequences obtained in this study.The results showed that the four P.kuehnii sequences obtained in this study were clustered with Puccinia polysora(GenBank accession number:GU058024)into one group,and they were close genetically;the 53 P.melanocephala sequences were clustered with P.nakanishikii(GenBank accession number:GU058002),P.rufipes(GenBank accession number:AJ296545),Aecidium deutziae(GenBank accession number:KU309317)and P.coronata(GenBank accession number:DQ354526)into one group,and they had a close genetic relationship;and the genetic relationship of the two species of sugarcane rust fungi identified in this study was relatively distant.[Conclusions]This study provides a scientific basis for sugarcane rust epidemic prediction,disease resistance breeding,and precise prevention and control.展开更多
Toxicity of 96% chlorpyrifos TC, 98% imidacloprid TC, 93% thiamethoxam TC and mixed pesticides of chlorpyrifos with other two agents （D ＋ B, D ＋ S） to C. lanigera （Zehntner） were determined under laboratory cond...Toxicity of 96% chlorpyrifos TC, 98% imidacloprid TC, 93% thiamethoxam TC and mixed pesticides of chlorpyrifos with other two agents （D ＋ B, D ＋ S） to C. lanigera （Zehntner） were determined under laboratory conditions by leaf dipping method. The results showed that all of the pesticides had high toxicity with the order of thiamethoxam TC 〉 imidacloprid TC 〉 D ＋ B 〉 chlorpyrifos TC 〉 D ＋ S. The results provided theoretic basis for selecting suitable insecti- cides to control the C. lanigera （Zehntner）.展开更多
基金Supported by China Agriculture Research System of MOF and MARA(CARS-170303)Joint Special Project of Basic Agricultural Research in Yunnan Province(2018FG001-029)+1 种基金"Yunling Industrial Technology Leading Talents"Training Project(2018LJRC56)Special Fund for the Construction of Modern Agricultural Industry Technology System in Yunnan Province(YNGZTX-4-92)。
文摘[Objectives]This study was conducted to clarify the species,occurrence and distribution characteristics of sugarcane rust pathogens in low-latitude plateau sugarcane rust areas of Yunnan Province and the phylogenetic relationship between the pathogens and that between them and other rust fungi of the genus Puccinia.[Methods]Fifty seven sugarcane rust samples collected from the sugarcane areas of Baoshan,Lincang,Menghai,Menglian,Ximeng,Lancang and Wenshan in Yunnan were subjected to molecular identification.[Results]The four sugarcane rust samples from Haiyin1 hao in Menghai,Yunnan Province belonged to orange rust of sugarcane,and the pathogen was Puccinia kuehnii Butler.;and other 53 samples of sugarcane rust belonged to brown rust of sugarcane,and the pathogen was Puccinia melanocephala Syd.et P.Syd.The rDNA sequences of other rust fungi from the genus Puccinia were downloaded from GenBank and used for the construction of an NJ tree for systematic evolution analysis together with the sequences obtained in this study.The results showed that the four P.kuehnii sequences obtained in this study were clustered with Puccinia polysora(GenBank accession number:GU058024)into one group,and they were close genetically;the 53 P.melanocephala sequences were clustered with P.nakanishikii(GenBank accession number:GU058002),P.rufipes(GenBank accession number:AJ296545),Aecidium deutziae(GenBank accession number:KU309317)and P.coronata(GenBank accession number:DQ354526)into one group,and they had a close genetic relationship;and the genetic relationship of the two species of sugarcane rust fungi identified in this study was relatively distant.[Conclusions]This study provides a scientific basis for sugarcane rust epidemic prediction,disease resistance breeding,and precise prevention and control.
基金Sponsored by Undergraduate Innovative Venture Program of Guizhou University(20120427)Study and Application of High-efficiency Safe Control Technology for Major Sugarcane Diseases and Pests(NY[2011]3024)
文摘Toxicity of 96% chlorpyrifos TC, 98% imidacloprid TC, 93% thiamethoxam TC and mixed pesticides of chlorpyrifos with other two agents （D ＋ B, D ＋ S） to C. lanigera （Zehntner） were determined under laboratory conditions by leaf dipping method. The results showed that all of the pesticides had high toxicity with the order of thiamethoxam TC 〉 imidacloprid TC 〉 D ＋ B 〉 chlorpyrifos TC 〉 D ＋ S. The results provided theoretic basis for selecting suitable insecti- cides to control the C. lanigera （Zehntner）.
