The present work was undertaken to study in hairdressers exposed to several irritants and allergens (prevalently hairdyeing) and affected by hand contact dermatitis the possible correlation between exposure and direct...The present work was undertaken to study in hairdressers exposed to several irritants and allergens (prevalently hairdyeing) and affected by hand contact dermatitis the possible correlation between exposure and direct- oxidative DNA damage, production of tumour necrosis factor alpha (TNFα ) and allergic inflammatory disease. We evaluated in 19 hairdressers with hand contact dermatitis, 14 allergic contact dermatitis (ACD) and 5 irritant contact dermatitis (ICD) and in a selected control group TNFα serum levels by ELISA and direct- oxidative DNA damage by Fpg (formamido- pyrimidine glycosylase) modified Comet test on blood. Hairdressers were divided on the basis of number of hair- dyeing carried out weekly into 2 groups: low- exposure (<60 hair- dyeing/week) and highexposure hairdressers (≥ 60 hair- dyeing/week) that reflect also the exposure to other allergens and irritants and 2 different tasks (hairdressers and apprentice hairdressers, respectively). Serum levels of TNFα in hairdressers with ACD were significantly higher than controls with a correlation to exposure level. Significant DNA damage in ICD hairdressers with higher exposure as compared to controls was found. These findings suggest that occupational exposure can induce in hairdressers, particularly ICD, DNA damage, increase the TNFa levels particularly in ACD and induce allergic sensitization, suggesting a relationship between direct- oxidativeDNA damage, TNFα production and allergic inflammatory disease.展开更多
In this study, we present a case of a patient who has not been occupationally exposed to reactive dyes, but did present with a dermatitis from wearing a dark cotton garment. The patient experienced reactivation of his...In this study, we present a case of a patient who has not been occupationally exposed to reactive dyes, but did present with a dermatitis from wearing a dark cotton garment. The patient experienced reactivation of his dermatitis when rewearing a new unwashed dark T- shirt made of 100% cotton (in fact, the patient reported that it had to be washed at least 3 times before the skin reaction disappeared). He presented positive patch tests to 6 reactive dyes from Chemotechnique textile series. The clothing could not be proved as the true cause of the dermatitis, but resolution occurred upon removal of the suspected garment. This suggests that contact allergy to the reactive dyes (he did not react to any other dyes and his garment was a natural fabric)was likely responsible. With this report,we would like to emphasize that reactive dyes, as a class, should be considered as potential allergens, both occupationally and from non- occupational exposure such as garments. If garments containing reactive dyes are not properly rinsed in the manufacturing process, we believe that excess of dye can be retained that may cause allergic contact dermatitis (ACD). As the reactive dyes and their hydrolysis products are very water- soluble, they can be easily washed off to prevent ACD.展开更多
The patch test results of 12 058 patients (4416 male and 7642 female) referred to 9 clinics in the Czech Republic between January 1997 and December 2001 were evaluated. Patients were tested with the same series of all...The patch test results of 12 058 patients (4416 male and 7642 female) referred to 9 clinics in the Czech Republic between January 1997 and December 2001 were evaluated. Patients were tested with the same series of allergens by using the standardized patch testing method. The current standard tool for diagnosing allergic contact dermatitis (ACD) in the Czech Republic is the Trolab test panel (Hermal, Reinbeck, Germany) which consists of 23 allergens. Only a few data exists on ACD in the Czech Republic. All patients were tested with the 23 allergen European standard series. Of these patients, 7661 (63.5% ) had 1 or more positive reactions. On average, there were 2.8 positive reactions per patient. ACD, according to clinical relevance, was diagnosed in 5339 (69.7% ) of these patients. The most frequent allergens were metals (22.9% ), especially nickel sulfate (13.8% ), and followed by Myroxylon pereirae resin (balsam of Peru) (7.3% ), fragrance mix (5.8% ), formaldehyde (4.2% ) and lanolin alcohol (3.0% ). Our results were compared with results from other countries. We conclude that the European standard series is suitable for detection of ACD in the Czech Republic.展开更多
文摘The present work was undertaken to study in hairdressers exposed to several irritants and allergens (prevalently hairdyeing) and affected by hand contact dermatitis the possible correlation between exposure and direct- oxidative DNA damage, production of tumour necrosis factor alpha (TNFα ) and allergic inflammatory disease. We evaluated in 19 hairdressers with hand contact dermatitis, 14 allergic contact dermatitis (ACD) and 5 irritant contact dermatitis (ICD) and in a selected control group TNFα serum levels by ELISA and direct- oxidative DNA damage by Fpg (formamido- pyrimidine glycosylase) modified Comet test on blood. Hairdressers were divided on the basis of number of hair- dyeing carried out weekly into 2 groups: low- exposure (<60 hair- dyeing/week) and highexposure hairdressers (≥ 60 hair- dyeing/week) that reflect also the exposure to other allergens and irritants and 2 different tasks (hairdressers and apprentice hairdressers, respectively). Serum levels of TNFα in hairdressers with ACD were significantly higher than controls with a correlation to exposure level. Significant DNA damage in ICD hairdressers with higher exposure as compared to controls was found. These findings suggest that occupational exposure can induce in hairdressers, particularly ICD, DNA damage, increase the TNFa levels particularly in ACD and induce allergic sensitization, suggesting a relationship between direct- oxidativeDNA damage, TNFα production and allergic inflammatory disease.
文摘In this study, we present a case of a patient who has not been occupationally exposed to reactive dyes, but did present with a dermatitis from wearing a dark cotton garment. The patient experienced reactivation of his dermatitis when rewearing a new unwashed dark T- shirt made of 100% cotton (in fact, the patient reported that it had to be washed at least 3 times before the skin reaction disappeared). He presented positive patch tests to 6 reactive dyes from Chemotechnique textile series. The clothing could not be proved as the true cause of the dermatitis, but resolution occurred upon removal of the suspected garment. This suggests that contact allergy to the reactive dyes (he did not react to any other dyes and his garment was a natural fabric)was likely responsible. With this report,we would like to emphasize that reactive dyes, as a class, should be considered as potential allergens, both occupationally and from non- occupational exposure such as garments. If garments containing reactive dyes are not properly rinsed in the manufacturing process, we believe that excess of dye can be retained that may cause allergic contact dermatitis (ACD). As the reactive dyes and their hydrolysis products are very water- soluble, they can be easily washed off to prevent ACD.
文摘The patch test results of 12 058 patients (4416 male and 7642 female) referred to 9 clinics in the Czech Republic between January 1997 and December 2001 were evaluated. Patients were tested with the same series of allergens by using the standardized patch testing method. The current standard tool for diagnosing allergic contact dermatitis (ACD) in the Czech Republic is the Trolab test panel (Hermal, Reinbeck, Germany) which consists of 23 allergens. Only a few data exists on ACD in the Czech Republic. All patients were tested with the 23 allergen European standard series. Of these patients, 7661 (63.5% ) had 1 or more positive reactions. On average, there were 2.8 positive reactions per patient. ACD, according to clinical relevance, was diagnosed in 5339 (69.7% ) of these patients. The most frequent allergens were metals (22.9% ), especially nickel sulfate (13.8% ), and followed by Myroxylon pereirae resin (balsam of Peru) (7.3% ), fragrance mix (5.8% ), formaldehyde (4.2% ) and lanolin alcohol (3.0% ). Our results were compared with results from other countries. We conclude that the European standard series is suitable for detection of ACD in the Czech Republic.
