目的:研究三氧化二砷白蛋白微球(As2O3 HAS NS)对人膀胱癌细胞生长的抑制作用。方法:用交 联固化的方法制备As2O3 HAS NS,用原子吸光光度法测量微球中的砷浓度,采用显微镜观察和MTT法研究 As2O3 HAS NS对细胞生长的抑制作用,用3氢...目的:研究三氧化二砷白蛋白微球(As2O3 HAS NS)对人膀胱癌细胞生长的抑制作用。方法:用交 联固化的方法制备As2O3 HAS NS,用原子吸光光度法测量微球中的砷浓度,采用显微镜观察和MTT法研究 As2O3 HAS NS对细胞生长的抑制作用,用3氢 胸腺嘧啶核苷(3H TDR)掺入法测定其体外杀伤活性,用流式细 胞术(FCM)检测细胞凋亡。结果:显微镜观察及MTT法显示As2O3 HAS NS对人膀胱癌EJ细胞体外生长有 明显抑制作用,呈现出时间及剂量依赖性。3H TDR掺入法测得As2O3 HAS NS作用的EJ细胞存活率开始较 高,但随着时间的延长,较As2O3降低快。流式细胞分析显示As2O3 HAS NS有明显地促进EJ细胞凋亡的作 用,G0/G1期百分比明显减少,而G2/M期百分比明显增多,至24h达57.1%。24hSub G1期细胞占9.4%。结 论:As2O3 HAS NS对EJ细胞的作用优于单纯As2O3,可能成为实体肿瘤治疗的更优剂型。展开更多
Objective： To explore the expression of caveolin-1 （CAV-1） and matrix metalloproteinase-2 （MMP-2）in bladder cancer and its clinical significance. Methods： The expression of CAV-1 and MMP-2 were detected by the S...Objective： To explore the expression of caveolin-1 （CAV-1） and matrix metalloproteinase-2 （MMP-2）in bladder cancer and its clinical significance. Methods： The expression of CAV-1 and MMP-2 were detected by the SP immunohistochemical method in 77 cases of bladder cancer. Results： The positive rates of CAV-1, MMP-2 in bladder transitional cell carcinoma （BTCC） and bladder adenocarcinoma were significantly high than those of normal bladder mucosa, the positive rates in the deeper cancer invasion was significantly high than those of superficial bladder cancer （P 〈 0.01）, the positive rates of CAV-1 in grades Ⅰ, Ⅱ and Ⅲ of bladder transitional cell carcinaoma （BTCC） were 15%, 40% and 68%, respectively; the positive rates of MMP-2 in grades Ⅰ, Ⅱ and Ⅲ of bladder transitional cell carcinoma （BTCC） were 20%, 40% and 72%, respectively （P 〈 0.01）. CAV-1 was positive associated with MMP-2 in bladder cancer （r = 0.598, P 〈 0.001）, but not in bladder tissue. Conclusion： CAV-1 and MMP-2 were associated with stage and grade of bladder cancer, which suggested that CAV-1 might improve secretion of MMP-2.展开更多
文摘目的:研究三氧化二砷白蛋白微球(As2O3 HAS NS)对人膀胱癌细胞生长的抑制作用。方法:用交 联固化的方法制备As2O3 HAS NS,用原子吸光光度法测量微球中的砷浓度,采用显微镜观察和MTT法研究 As2O3 HAS NS对细胞生长的抑制作用,用3氢 胸腺嘧啶核苷(3H TDR)掺入法测定其体外杀伤活性,用流式细 胞术(FCM)检测细胞凋亡。结果:显微镜观察及MTT法显示As2O3 HAS NS对人膀胱癌EJ细胞体外生长有 明显抑制作用,呈现出时间及剂量依赖性。3H TDR掺入法测得As2O3 HAS NS作用的EJ细胞存活率开始较 高,但随着时间的延长,较As2O3降低快。流式细胞分析显示As2O3 HAS NS有明显地促进EJ细胞凋亡的作 用,G0/G1期百分比明显减少,而G2/M期百分比明显增多,至24h达57.1%。24hSub G1期细胞占9.4%。结 论:As2O3 HAS NS对EJ细胞的作用优于单纯As2O3,可能成为实体肿瘤治疗的更优剂型。
文摘Objective： To explore the expression of caveolin-1 （CAV-1） and matrix metalloproteinase-2 （MMP-2）in bladder cancer and its clinical significance. Methods： The expression of CAV-1 and MMP-2 were detected by the SP immunohistochemical method in 77 cases of bladder cancer. Results： The positive rates of CAV-1, MMP-2 in bladder transitional cell carcinoma （BTCC） and bladder adenocarcinoma were significantly high than those of normal bladder mucosa, the positive rates in the deeper cancer invasion was significantly high than those of superficial bladder cancer （P 〈 0.01）, the positive rates of CAV-1 in grades Ⅰ, Ⅱ and Ⅲ of bladder transitional cell carcinaoma （BTCC） were 15%, 40% and 68%, respectively; the positive rates of MMP-2 in grades Ⅰ, Ⅱ and Ⅲ of bladder transitional cell carcinoma （BTCC） were 20%, 40% and 72%, respectively （P 〈 0.01）. CAV-1 was positive associated with MMP-2 in bladder cancer （r = 0.598, P 〈 0.001）, but not in bladder tissue. Conclusion： CAV-1 and MMP-2 were associated with stage and grade of bladder cancer, which suggested that CAV-1 might improve secretion of MMP-2.
