目的:评价可变硬度结肠镜(variable-stiffness colonoscope,VSC)在老年患者结肠镜筛查中的应用价值.方法:2012-07/2013-03在我院接受大肠癌筛查项目(60-74岁年龄组)行结肠镜检查的患者共732例,随机分为:可变硬度结肠镜(VSC组)和普通成...目的:评价可变硬度结肠镜(variable-stiffness colonoscope,VSC)在老年患者结肠镜筛查中的应用价值.方法:2012-07/2013-03在我院接受大肠癌筛查项目(60-74岁年龄组)行结肠镜检查的患者共732例,随机分为:可变硬度结肠镜(VSC组)和普通成人结肠镜(standard adult colonoscope,SAC)(SAC组),分别用OLYMPUS CF-H260和FUJINON 450WM5型结肠镜完成单人结肠镜插入.统计分析两组的达盲率、达盲时间、腹痛VAS评分、变换体位/腹部按压、追加镇静镇痛药物等指标.结果:VSC组的达盲率较高(95.9%vs 90.7%,P<0.05);同时,VSC组也较少需要在检查过程中变换体位和助手协助腹部按压(12.6%vs 33.3%,P<0.01);达盲时间VSC组和SAC组近似8.2 min±3.5 min vs 9.4 min±3.2 min,P>0.05;V S C组腹痛评分较S A C组高(57.4vs 44.1),但需要镇静镇痛几率类似(4.1%vs3.8%,P>0.05).结论:使用VSC可提高达盲率,并在插入过程中较少需要体位变换和助手协助,两组达盲时间相近,VSC腹痛等不适较SAC多见,但程度较轻,并不增加需要镇静镇痛的几率.展开更多
AIM: To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the...AIM: To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the immunoregulatory mechanisms. METHODS: Peripheral blood mononuclear cells (PBMNC) from UC patients or HT-29 cells were co-cultured with heat-killed probiotic bacteria or culture supernatant of Bifidobacterium breve strain Yakult (BbrY) or Bifidobacterium bifidum strain Yakult (BbiY) to estimate the amount of IL-10 or IL-8 secreted. RESULTS: Both strains of probiotic Bifidobacteria contained in the BFM induced IL-10 production in PBMNC from UC patients, though BbrY was more effective than BbiY. Conditioned medium (CM) and DNA of both strains inhibited IL-8 secretion in HT-29 cells stimulated with TNF-α, whereas no such effect was observed with heat- killed bacteria. The inhibitory effect of CM derived from BbiY was greater than that of CM derived from BbrY. DNAs of the two strains had a comparable inhibitory activity against the secretion of IL-8. CM of BbiY induced a repression of IL-8 gene expression with a higher expression of IκB-ζ mRNA 4 h after culture of HT-29 cells compared to that in the absence of CM.CONCLUSION: Probiotic Bifidobacterium strains in BFM enhance IL-10 production in PBMNC and inhibit IL-8 secretion in intestinal epithelial cells, suggesting that BFM has anti-inflammatory effects against ulcerative colitis.展开更多
文摘目的:评价可变硬度结肠镜(variable-stiffness colonoscope,VSC)在老年患者结肠镜筛查中的应用价值.方法:2012-07/2013-03在我院接受大肠癌筛查项目(60-74岁年龄组)行结肠镜检查的患者共732例,随机分为:可变硬度结肠镜(VSC组)和普通成人结肠镜(standard adult colonoscope,SAC)(SAC组),分别用OLYMPUS CF-H260和FUJINON 450WM5型结肠镜完成单人结肠镜插入.统计分析两组的达盲率、达盲时间、腹痛VAS评分、变换体位/腹部按压、追加镇静镇痛药物等指标.结果:VSC组的达盲率较高(95.9%vs 90.7%,P<0.05);同时,VSC组也较少需要在检查过程中变换体位和助手协助腹部按压(12.6%vs 33.3%,P<0.01);达盲时间VSC组和SAC组近似8.2 min±3.5 min vs 9.4 min±3.2 min,P>0.05;V S C组腹痛评分较S A C组高(57.4vs 44.1),但需要镇静镇痛几率类似(4.1%vs3.8%,P>0.05).结论:使用VSC可提高达盲率,并在插入过程中较少需要体位变换和助手协助,两组达盲时间相近,VSC腹痛等不适较SAC多见,但程度较轻,并不增加需要镇静镇痛的几率.
文摘AIM: To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the immunoregulatory mechanisms. METHODS: Peripheral blood mononuclear cells (PBMNC) from UC patients or HT-29 cells were co-cultured with heat-killed probiotic bacteria or culture supernatant of Bifidobacterium breve strain Yakult (BbrY) or Bifidobacterium bifidum strain Yakult (BbiY) to estimate the amount of IL-10 or IL-8 secreted. RESULTS: Both strains of probiotic Bifidobacteria contained in the BFM induced IL-10 production in PBMNC from UC patients, though BbrY was more effective than BbiY. Conditioned medium (CM) and DNA of both strains inhibited IL-8 secretion in HT-29 cells stimulated with TNF-α, whereas no such effect was observed with heat- killed bacteria. The inhibitory effect of CM derived from BbiY was greater than that of CM derived from BbrY. DNAs of the two strains had a comparable inhibitory activity against the secretion of IL-8. CM of BbiY induced a repression of IL-8 gene expression with a higher expression of IκB-ζ mRNA 4 h after culture of HT-29 cells compared to that in the absence of CM.CONCLUSION: Probiotic Bifidobacterium strains in BFM enhance IL-10 production in PBMNC and inhibit IL-8 secretion in intestinal epithelial cells, suggesting that BFM has anti-inflammatory effects against ulcerative colitis.
