Activities of the laccase and polyphenol oxidase of Corilus versicolor cultured in PDY fluid medium were studied. The peak of enzyme production appeared in the 9th day for laccase and 14th for polyphenol oxidase in th...Activities of the laccase and polyphenol oxidase of Corilus versicolor cultured in PDY fluid medium were studied. The peak of enzyme production appeared in the 9th day for laccase and 14th for polyphenol oxidase in the constant temperature vibration of 30℃ and 110r/min and the maxmum enzyme activity of the former was 395.6u and the later was 549.0u. The optimum temperature and pH were 25℃ and 4.6 for laccase and 30℃ and 5.0 for polyphenol oxidase. The k + and Zn 2+ were activators for laccase and Mn 2+ ,Ba 2+ , Mg 2+ for polyphenol oxidase, wheras Fe 3+ and Ag + were inhbited for both of them.展开更多
文摘Activities of the laccase and polyphenol oxidase of Corilus versicolor cultured in PDY fluid medium were studied. The peak of enzyme production appeared in the 9th day for laccase and 14th for polyphenol oxidase in the constant temperature vibration of 30℃ and 110r/min and the maxmum enzyme activity of the former was 395.6u and the later was 549.0u. The optimum temperature and pH were 25℃ and 4.6 for laccase and 30℃ and 5.0 for polyphenol oxidase. The k + and Zn 2+ were activators for laccase and Mn 2+ ,Ba 2+ , Mg 2+ for polyphenol oxidase, wheras Fe 3+ and Ag + were inhbited for both of them.
