To predict the subcellular location of ascorbate peroxidase 1 （ APX1 ） protein in Arabidopsis thaliana, a plasmid expressing APXl-enbance green fluores- cent protein （EGFP） fusion protein was constructed, transfor...To predict the subcellular location of ascorbate peroxidase 1 （ APX1 ） protein in Arabidopsis thaliana, a plasmid expressing APXl-enbance green fluores- cent protein （EGFP） fusion protein was constructed, transformed into Agrobacterium tumefaciens GV3101, and transiently expressed in tobacco leaves. The localization of APX1 protein in the cells was determined by detecting the distribution of the fusion protein in tobacco epidermal cells. The results showed APX1 protein was mainly distributed in the cytoplasm of the plant.展开更多
Biological Sciences have entered the genomeera.“Changes that will have effects comparable tothose of the Industrial Revolution and the Comput-er-based Revolution are now beginning.The nextgreat era,a genomics revolut...Biological Sciences have entered the genomeera.“Changes that will have effects comparable tothose of the Industrial Revolution and the Comput-er-based Revolution are now beginning.The nextgreat era,a genomics revolution,is in an earlyphase”(Science,Vol.279 p2019,1998).The展开更多
Maize（Zea mays L.）is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013（Baldaufa et al.,2016）.Heterosis is widely used to increase the productivity of maize,and the first ...Maize（Zea mays L.）is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013（Baldaufa et al.,2016）.Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s（Duvick,2001）.展开更多
DCAF (DDB1-CUL4 Associated Factor)是以CUL4 (CULLIN4)蛋白为骨架的E3泛素-蛋白连接酶复合体,这类复合体通过一个共同的衔接蛋白DDB1 (UV-damaged DNA binding protein 1)连接不同的底物识别亚基DCAF,介导各自特定的蛋白底物的降解,...DCAF (DDB1-CUL4 Associated Factor)是以CUL4 (CULLIN4)蛋白为骨架的E3泛素-蛋白连接酶复合体,这类复合体通过一个共同的衔接蛋白DDB1 (UV-damaged DNA binding protein 1)连接不同的底物识别亚基DCAF,介导各自特定的蛋白底物的降解,从而参与调控了多种生物学过程。为分析DCAF基因在白花虎眼万年青叶上珠芽、叶片和鳞茎等器官的功能和作用,本研究首次从百合科植物白花虎眼万年青叶上珠芽中分离出DCAF基因家族重要成员,进行氨基酸保守序列比对及系统进化分析后,将其命名为OtDCAF8基因;将其置于UBI启动子下在烟草中超量表达,转基因烟草的叶片形状、叶脉等发生了明显的改变。结果表明,OtDCAF8基因对植物干细胞及器官再生发育具有明显调控作用,可能在白花虎眼万年青的珠芽和叶片等器官发育过程中发挥着重要作用。本研究为深入探讨白花虎眼万年青珠芽等器官发育的分子机制和DCAF基因的功能提供了参考和依据,为百合等花卉的分子育种提供了有价值的重要功能基因。展开更多
非生物胁迫严重影响植物的生长发育及农作物的产量。植物激素脱落酸(ABA,abscisic acid)是植物响应非生物胁迫的重要信号分子,其介导的ABA信号途径在植物应答非生物胁迫过程中发挥关键作用,其中ABF(ABA-responsive element binding fact...非生物胁迫严重影响植物的生长发育及农作物的产量。植物激素脱落酸(ABA,abscisic acid)是植物响应非生物胁迫的重要信号分子,其介导的ABA信号途径在植物应答非生物胁迫过程中发挥关键作用,其中ABF(ABA-responsive element binding factors)转录因子在ABA信号途径中扮演着重要的角色。ABF转录因子是一类特异识别ABA响应元件(ABRE,ABA-responsive element)的碱性亮氨酸拉链蛋白,属于bZIP家族中的A亚族。它含有5个高度保守的结构域,C1、C2、C3、C4以及可以结合DNA序列的bZIP区域,这些保守结构域能够被蔗糖非酵解型蛋白激酶2(SnRK2,sucrose nonfermenting-1 related protein kinase 2)和钙依赖蛋白激酶(CDPK,calcium-dependent protein kinase)等多种蛋白激酶磷酸化,进而激活ABF蛋白的转录活性,使其参与ABA和逆境胁迫的调控表达。近年来,有较多研究发现不同物种中的ABF类转录因子在植物响应非生物胁迫的过程中具有重要作用。本文综述了ABF转录因子的结构特征及识别的顺式作用元件如G-ABRE、C-ABRE、"偶联元件3"(CE3,coupling element 3)等、调控修饰途径及其在盐、干旱、低温等非生物胁迫应答中的作用,并对未来ABF转录因子的研究方向进行展望,为通过调控ABF转录因子从而培育优良抗逆作物品种提供研究思路。展开更多
基金Supported by the Undergraduate Innovation and Entrepreneurship Training Program of Anhui Province(2016CXCYS099)
文摘To predict the subcellular location of ascorbate peroxidase 1 （ APX1 ） protein in Arabidopsis thaliana, a plasmid expressing APXl-enbance green fluores- cent protein （EGFP） fusion protein was constructed, transformed into Agrobacterium tumefaciens GV3101, and transiently expressed in tobacco leaves. The localization of APX1 protein in the cells was determined by detecting the distribution of the fusion protein in tobacco epidermal cells. The results showed APX1 protein was mainly distributed in the cytoplasm of the plant.
文摘Biological Sciences have entered the genomeera.“Changes that will have effects comparable tothose of the Industrial Revolution and the Comput-er-based Revolution are now beginning.The nextgreat era,a genomics revolution,is in an earlyphase”(Science,Vol.279 p2019,1998).The
基金supported by grants from the National Natural Science Foundation of China (Nos. 31501376 and 31570369)the National Key Research and Development Program of China (No. 2016YFD0101804)the National Transgenic Science and Technology Program (No. 2016ZX08010002)
文摘Maize（Zea mays L.）is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013（Baldaufa et al.,2016）.Heterosis is widely used to increase the productivity of maize,and the first commercial hybrid maize was introduced in the 1930s（Duvick,2001）.
文摘DCAF (DDB1-CUL4 Associated Factor)是以CUL4 (CULLIN4)蛋白为骨架的E3泛素-蛋白连接酶复合体,这类复合体通过一个共同的衔接蛋白DDB1 (UV-damaged DNA binding protein 1)连接不同的底物识别亚基DCAF,介导各自特定的蛋白底物的降解,从而参与调控了多种生物学过程。为分析DCAF基因在白花虎眼万年青叶上珠芽、叶片和鳞茎等器官的功能和作用,本研究首次从百合科植物白花虎眼万年青叶上珠芽中分离出DCAF基因家族重要成员,进行氨基酸保守序列比对及系统进化分析后,将其命名为OtDCAF8基因;将其置于UBI启动子下在烟草中超量表达,转基因烟草的叶片形状、叶脉等发生了明显的改变。结果表明,OtDCAF8基因对植物干细胞及器官再生发育具有明显调控作用,可能在白花虎眼万年青的珠芽和叶片等器官发育过程中发挥着重要作用。本研究为深入探讨白花虎眼万年青珠芽等器官发育的分子机制和DCAF基因的功能提供了参考和依据,为百合等花卉的分子育种提供了有价值的重要功能基因。
文摘非生物胁迫严重影响植物的生长发育及农作物的产量。植物激素脱落酸(ABA,abscisic acid)是植物响应非生物胁迫的重要信号分子,其介导的ABA信号途径在植物应答非生物胁迫过程中发挥关键作用,其中ABF(ABA-responsive element binding factors)转录因子在ABA信号途径中扮演着重要的角色。ABF转录因子是一类特异识别ABA响应元件(ABRE,ABA-responsive element)的碱性亮氨酸拉链蛋白,属于bZIP家族中的A亚族。它含有5个高度保守的结构域,C1、C2、C3、C4以及可以结合DNA序列的bZIP区域,这些保守结构域能够被蔗糖非酵解型蛋白激酶2(SnRK2,sucrose nonfermenting-1 related protein kinase 2)和钙依赖蛋白激酶(CDPK,calcium-dependent protein kinase)等多种蛋白激酶磷酸化,进而激活ABF蛋白的转录活性,使其参与ABA和逆境胁迫的调控表达。近年来,有较多研究发现不同物种中的ABF类转录因子在植物响应非生物胁迫的过程中具有重要作用。本文综述了ABF转录因子的结构特征及识别的顺式作用元件如G-ABRE、C-ABRE、"偶联元件3"(CE3,coupling element 3)等、调控修饰途径及其在盐、干旱、低温等非生物胁迫应答中的作用,并对未来ABF转录因子的研究方向进行展望,为通过调控ABF转录因子从而培育优良抗逆作物品种提供研究思路。
