Background Peripheral skeletal muscle dysfunction in patients with chronic obstructive pulmonary disease （COPD） may be due to the disease per se or as a result of concomitant confounding factors. Although the mechan...Background Peripheral skeletal muscle dysfunction in patients with chronic obstructive pulmonary disease （COPD） may be due to the disease per se or as a result of concomitant confounding factors. Although the mechanistic basis for this functional impairment is uncertain, oxidative stress may play a role. The purpose of this study was to investigate whether local oxidative stress is associated with the reduced peripheral skeletal muscle performance in rats with emphysema. Methods In situ mechanical properties of gastrocnemius were measured in Sprague-Dawley rats 5 months after intratracheal instillation of either elastase （EMP, n=-10） or normal saline （CON, n=10）. Lipofuscin inclusions, myocyte apoptosis and antioxidant enzyme activities were examined in the gastrocnemius muscle. Results Lipofuscin inclusions were significantly higher in the gastrocnemius muscle of EMP compared with CON （3.2_--,-0.4 vs. 1.7_＋0.4, P〈0.01 ）. The activities of antioxidant enzymes were significantly increased in muscle homogenates of EMP as compared to CON. No significant differences were found in myocyte apoptosis between EMP and CON （1.2±0.9 vs. 1.0±0.8, P 〉0.05）. EMP decreased the fatigue endurance of gastrocnemius muscle （half-time to fatigue recovery： （150.0±55.4） seconds vs. （55.2±29.3） seconds, P 〈0.01） and had no effect on maximal tetanic force （（467.4±36.6） g vs. （493.2±30.5） g, P 〉0.05）. A significantly positive correlation was found between the level of lipofuscin inclusions and the half-time to fatigue recovery of gastrocnemius muscle in EMP （r=0.664, P 〈0.05）. Conclusion Local oxidative stress may have important functional consequences for peripheral skeletal muscle in rats with EMP.展开更多
最近7年来,一个外观简明、极其微小、异常短命而富有毒性的无机分子——一氧化氮(NO),正日益受到生命科学界和医学界的广泛关注。NO 研究的昨天1987年以前,人们并不了解 NO 还能在生物体内存在并有重要作用。然而,NO 客观上被应用和观...最近7年来,一个外观简明、极其微小、异常短命而富有毒性的无机分子——一氧化氮(NO),正日益受到生命科学界和医学界的广泛关注。NO 研究的昨天1987年以前,人们并不了解 NO 还能在生物体内存在并有重要作用。然而,NO 客观上被应用和观察的历史却长达一个多世纪。19世纪初,戴维(H.Davy)在研究'笑气'时意外发现 NO 及其毒性。此后 NO展开更多
文摘Background Peripheral skeletal muscle dysfunction in patients with chronic obstructive pulmonary disease （COPD） may be due to the disease per se or as a result of concomitant confounding factors. Although the mechanistic basis for this functional impairment is uncertain, oxidative stress may play a role. The purpose of this study was to investigate whether local oxidative stress is associated with the reduced peripheral skeletal muscle performance in rats with emphysema. Methods In situ mechanical properties of gastrocnemius were measured in Sprague-Dawley rats 5 months after intratracheal instillation of either elastase （EMP, n=-10） or normal saline （CON, n=10）. Lipofuscin inclusions, myocyte apoptosis and antioxidant enzyme activities were examined in the gastrocnemius muscle. Results Lipofuscin inclusions were significantly higher in the gastrocnemius muscle of EMP compared with CON （3.2_--,-0.4 vs. 1.7_＋0.4, P〈0.01 ）. The activities of antioxidant enzymes were significantly increased in muscle homogenates of EMP as compared to CON. No significant differences were found in myocyte apoptosis between EMP and CON （1.2±0.9 vs. 1.0±0.8, P 〉0.05）. EMP decreased the fatigue endurance of gastrocnemius muscle （half-time to fatigue recovery： （150.0±55.4） seconds vs. （55.2±29.3） seconds, P 〈0.01） and had no effect on maximal tetanic force （（467.4±36.6） g vs. （493.2±30.5） g, P 〉0.05）. A significantly positive correlation was found between the level of lipofuscin inclusions and the half-time to fatigue recovery of gastrocnemius muscle in EMP （r=0.664, P 〈0.05）. Conclusion Local oxidative stress may have important functional consequences for peripheral skeletal muscle in rats with EMP.
文摘最近7年来,一个外观简明、极其微小、异常短命而富有毒性的无机分子——一氧化氮(NO),正日益受到生命科学界和医学界的广泛关注。NO 研究的昨天1987年以前,人们并不了解 NO 还能在生物体内存在并有重要作用。然而,NO 客观上被应用和观察的历史却长达一个多世纪。19世纪初,戴维(H.Davy)在研究'笑气'时意外发现 NO 及其毒性。此后 NO
