Background: Gastric cancer (GC) is one of the most prevalent malignancies in the world today, with a high mortality rate. CDX2 is a Drosophila caudal-related homeobox transcription factor that plays an important ro...Background: Gastric cancer (GC) is one of the most prevalent malignancies in the world today, with a high mortality rate. CDX2 is a Drosophila caudal-related homeobox transcription factor that plays an important role in GC. Phosphatase and tensin homologue deleted from chromosome 10 (PTEN) is an important tumor suppressor which is widely expressed in normal human tissues. The aim of the study was to determine the relationship and mechanism between CDX2 and PTEN in invasion and migration of GC cells.Methods: pcDNA3-CDX2 plasr^lids were transfected into MGC-803 cells to up-regulate CDX2 protein, and small interfering RNA-CDX2 was transfected to down-regulate CDX2. The influence of CDX2 or PTEN on cell migration and invasion was measured by invasion, migration and wound healing assays. Western blotting assay and immunofluorescence were used to detect the expression ofCDX2, PTEN, phosphorylation ofAkt, E-cadherin and N-cadherin. Statistical significance was determined by one-way analysis of variance.Results: The results showed that CDX2 reduced the migration and invasion of GC cells (P 〈 0.05), and inhibited the activity of Akt through down-regulating PTEN expression (P 〈 0.05). CDX2 also restrained epithelial-mesenchymal transition of GC cells.Conclusions: CDX2 inhibited invasion andmigration of GC cells by PTEN/Akt signaling pathway, and that may be used for potential therapeutic target.展开更多
腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)是调节细胞能量代谢的关键酶。近年来研究还发现AMPK可通过抑制p53降解、上调Bim表达、抑制m TOR活性及升高活性氧(ROS)水平等途径促进细胞凋亡。本文综述了近年来关于AMPK促细...腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)是调节细胞能量代谢的关键酶。近年来研究还发现AMPK可通过抑制p53降解、上调Bim表达、抑制m TOR活性及升高活性氧(ROS)水平等途径促进细胞凋亡。本文综述了近年来关于AMPK促细胞凋亡机制的研究进展。展开更多
Background: Ovarian cancer is a leading gynecological malignancy. We investigated the prognostic value of programmed cell death 5 (PDCD5) in patients with ovarian cancer. Methods: Expression levels of PDCD5 mRNA a...Background: Ovarian cancer is a leading gynecological malignancy. We investigated the prognostic value of programmed cell death 5 (PDCD5) in patients with ovarian cancer. Methods: Expression levels of PDCD5 mRNA and protein were examined in six ovarian cancer cell lines (SKOV3, CAOV3, ES2, OV1, 3AO, and HOC 1 A) and one normal ovarian epithelial cell line (T29) using reverse transcription polymerase chain reaction, Western blotting, and flow cytolnetry. Alter inducing PDCD5 induction in SKOV3 cells or treating this cell line with taxol or doxorubicin (either alone or combined), apoptosis was measured by Annexin V-FITC/propidium iodide staining. Correlations between PDCD5 protein expression and pathological features, histological grade, FIGO stage, effective cytoreductive surgery, and serum cancer antigen-125 values were evaluated in patients with ovarian cancer. Results: PDCD5 mRNA and protein expression were downregulated in ovarian cancer cells. Recombinant human PDCD5 increased doxorubicin-induced apoptosis in SKOV3 cells (15.96 ± 2.07%, vs. 3.17 ± 1.45% in controls). In patients with ovarian cancer, PDCD5 expression was inversely correlated with FIGO stage, pathological grade, and patient survival (P 〈 0.05, R = 0.7139 for survival). Conclusions: PDCD5 expression is negatively correlated with disease progression and stage in ovarian cancer. Therefore, measuring PDCD5 expression may be a good method of determining the prognosis of ovarian cancer patients.展开更多
基金This study was supported by grants from the Beijing Natural Science Foundation
文摘Background: Gastric cancer (GC) is one of the most prevalent malignancies in the world today, with a high mortality rate. CDX2 is a Drosophila caudal-related homeobox transcription factor that plays an important role in GC. Phosphatase and tensin homologue deleted from chromosome 10 (PTEN) is an important tumor suppressor which is widely expressed in normal human tissues. The aim of the study was to determine the relationship and mechanism between CDX2 and PTEN in invasion and migration of GC cells.Methods: pcDNA3-CDX2 plasr^lids were transfected into MGC-803 cells to up-regulate CDX2 protein, and small interfering RNA-CDX2 was transfected to down-regulate CDX2. The influence of CDX2 or PTEN on cell migration and invasion was measured by invasion, migration and wound healing assays. Western blotting assay and immunofluorescence were used to detect the expression ofCDX2, PTEN, phosphorylation ofAkt, E-cadherin and N-cadherin. Statistical significance was determined by one-way analysis of variance.Results: The results showed that CDX2 reduced the migration and invasion of GC cells (P 〈 0.05), and inhibited the activity of Akt through down-regulating PTEN expression (P 〈 0.05). CDX2 also restrained epithelial-mesenchymal transition of GC cells.Conclusions: CDX2 inhibited invasion andmigration of GC cells by PTEN/Akt signaling pathway, and that may be used for potential therapeutic target.
文摘Background: Ovarian cancer is a leading gynecological malignancy. We investigated the prognostic value of programmed cell death 5 (PDCD5) in patients with ovarian cancer. Methods: Expression levels of PDCD5 mRNA and protein were examined in six ovarian cancer cell lines (SKOV3, CAOV3, ES2, OV1, 3AO, and HOC 1 A) and one normal ovarian epithelial cell line (T29) using reverse transcription polymerase chain reaction, Western blotting, and flow cytolnetry. Alter inducing PDCD5 induction in SKOV3 cells or treating this cell line with taxol or doxorubicin (either alone or combined), apoptosis was measured by Annexin V-FITC/propidium iodide staining. Correlations between PDCD5 protein expression and pathological features, histological grade, FIGO stage, effective cytoreductive surgery, and serum cancer antigen-125 values were evaluated in patients with ovarian cancer. Results: PDCD5 mRNA and protein expression were downregulated in ovarian cancer cells. Recombinant human PDCD5 increased doxorubicin-induced apoptosis in SKOV3 cells (15.96 ± 2.07%, vs. 3.17 ± 1.45% in controls). In patients with ovarian cancer, PDCD5 expression was inversely correlated with FIGO stage, pathological grade, and patient survival (P 〈 0.05, R = 0.7139 for survival). Conclusions: PDCD5 expression is negatively correlated with disease progression and stage in ovarian cancer. Therefore, measuring PDCD5 expression may be a good method of determining the prognosis of ovarian cancer patients.