目的:评估强脉冲激光(IPL)治疗中重度睑板腺功能障碍(MGD)相关性干眼患者的短期疗效及对心理状况的影响。方法:自身前后对照。收集2018-10/2019-10就诊于武汉大学人民医院眼科门诊的中重度MGD患者25例50眼,给予IPL联合睑板腺按摩治疗。...目的:评估强脉冲激光(IPL)治疗中重度睑板腺功能障碍(MGD)相关性干眼患者的短期疗效及对心理状况的影响。方法:自身前后对照。收集2018-10/2019-10就诊于武汉大学人民医院眼科门诊的中重度MGD患者25例50眼,给予IPL联合睑板腺按摩治疗。于治疗前和治疗一个疗程后1mo评估患者症状、体征及心理状态的变化,采用眼表疾病指数(OSDI)问卷评分标准评估患者症状,泪液分泌试验(SⅠt)、泪河高度、平均泪膜破裂时间(Mean BUT)、睑板腺缺失程度以及欧卡斯双通道系统测量平均散射指数(Mean OSI)来评估患者的体征,使用焦虑自评量表(SAS)和流调用抑郁自评量表(CES-D)来评估患者的心理状态。结果:患者治疗前后OSDI评分(58.36±10.34 vs 49.12±8.05分)、SⅠt(5.64±1.6 vs 8.24±1.71mm/5min)、泪河高度(0.19±0.04 vs 0.23±0.04mm)、Mean BUT(5.65±1.72 vs 7.06±1.84s)、Mean OSI(0.89±0.24 vs 0.75±0.23)、睑板腺缺失程度评分(2.56±0.58 vs 1.84±0.85分)、SAS评分(46.92±8.49 vs 40.2±8.01分),CES-D评分(21.04±6.11 vs 16.92±4.24分),所有指标治疗前后差异均有统计学意义(P<0.01)。OSDI评分与Mean BUT呈负相关(rs=-0.511,P<0.01),与Mean OSI呈正相关(rs=0.479,P<0.05),OSDI评分与SAS评分及CES-D评分均呈正相关(rs=0.775,0.856,均P<0.01),SAS评分与SⅠt和Mean BUT呈负相关(rs=-0.653,-0.713,均P<0.01),SAS与CES-D评分呈正相关(rs=0.730,P<0.01)。结论:短期观察IPL治疗中重度MGD相关性干眼安全、有效,治疗后患者干眼症状、体征及心理状态得到不同程度的缓解,患者的抑郁和焦虑与眼表症状相关。展开更多
Objective： Light quality has effect on the accumulation of gypenosides in the medicinal plant Gynosternma pentaphyllum in the family Cucurbitaceae, while the squalene synthase （SS） and squalene epoxidase （SE） are...Objective： Light quality has effect on the accumulation of gypenosides in the medicinal plant Gynosternma pentaphyllum in the family Cucurbitaceae, while the squalene synthase （SS） and squalene epoxidase （SE） are the key enzymes for gypenoside biosynthesis, The objective of this study was to elucidate the rela- tionship between light quality and biosynthesis key enzyme involving the regulation of gypenoside accu- mulation. Methods： The content of total gypenosides was measured by colorimetric method and the expression of SS and SE gene was determined by quantitative Real-time PCR in the seedlings of G. pentaphyllum which were grown with different light quality. Results： Light quality showed remarkable impacts on the accumulation of total gypenosides. The highest content of total gypenosides in the plant under red light condition was determined, followed by blue light and white light, while the lowest content was recorded under dark condition, qRT-PCR analysis proved that the expression levels of SS and SE genes were also affected by light quality. The high-level gene expressions of SS and SE were found in the plant under red light condition, followed by blue light, with the least content in darkness. The statistical analysis revealed that the total gypenosides were significantly different in different light treatment and the content of total gypenosides was positively related to the expression of SS and SE genes. Conclusions： Light quality regulates gypenoside accumulation via altering the expression of SS and SE in G. pentaphyllum.展开更多
文摘目的:评估强脉冲激光(IPL)治疗中重度睑板腺功能障碍(MGD)相关性干眼患者的短期疗效及对心理状况的影响。方法:自身前后对照。收集2018-10/2019-10就诊于武汉大学人民医院眼科门诊的中重度MGD患者25例50眼,给予IPL联合睑板腺按摩治疗。于治疗前和治疗一个疗程后1mo评估患者症状、体征及心理状态的变化,采用眼表疾病指数(OSDI)问卷评分标准评估患者症状,泪液分泌试验(SⅠt)、泪河高度、平均泪膜破裂时间(Mean BUT)、睑板腺缺失程度以及欧卡斯双通道系统测量平均散射指数(Mean OSI)来评估患者的体征,使用焦虑自评量表(SAS)和流调用抑郁自评量表(CES-D)来评估患者的心理状态。结果:患者治疗前后OSDI评分(58.36±10.34 vs 49.12±8.05分)、SⅠt(5.64±1.6 vs 8.24±1.71mm/5min)、泪河高度(0.19±0.04 vs 0.23±0.04mm)、Mean BUT(5.65±1.72 vs 7.06±1.84s)、Mean OSI(0.89±0.24 vs 0.75±0.23)、睑板腺缺失程度评分(2.56±0.58 vs 1.84±0.85分)、SAS评分(46.92±8.49 vs 40.2±8.01分),CES-D评分(21.04±6.11 vs 16.92±4.24分),所有指标治疗前后差异均有统计学意义(P<0.01)。OSDI评分与Mean BUT呈负相关(rs=-0.511,P<0.01),与Mean OSI呈正相关(rs=0.479,P<0.05),OSDI评分与SAS评分及CES-D评分均呈正相关(rs=0.775,0.856,均P<0.01),SAS评分与SⅠt和Mean BUT呈负相关(rs=-0.653,-0.713,均P<0.01),SAS与CES-D评分呈正相关(rs=0.730,P<0.01)。结论:短期观察IPL治疗中重度MGD相关性干眼安全、有效,治疗后患者干眼症状、体征及心理状态得到不同程度的缓解,患者的抑郁和焦虑与眼表症状相关。
基金financially supported by the National Natural Science Foundation of China (31760044,31260039)Key Course of Hunan Province (Ecology),Jishou University (2015005)
文摘Objective： Light quality has effect on the accumulation of gypenosides in the medicinal plant Gynosternma pentaphyllum in the family Cucurbitaceae, while the squalene synthase （SS） and squalene epoxidase （SE） are the key enzymes for gypenoside biosynthesis, The objective of this study was to elucidate the rela- tionship between light quality and biosynthesis key enzyme involving the regulation of gypenoside accu- mulation. Methods： The content of total gypenosides was measured by colorimetric method and the expression of SS and SE gene was determined by quantitative Real-time PCR in the seedlings of G. pentaphyllum which were grown with different light quality. Results： Light quality showed remarkable impacts on the accumulation of total gypenosides. The highest content of total gypenosides in the plant under red light condition was determined, followed by blue light and white light, while the lowest content was recorded under dark condition, qRT-PCR analysis proved that the expression levels of SS and SE genes were also affected by light quality. The high-level gene expressions of SS and SE were found in the plant under red light condition, followed by blue light, with the least content in darkness. The statistical analysis revealed that the total gypenosides were significantly different in different light treatment and the content of total gypenosides was positively related to the expression of SS and SE genes. Conclusions： Light quality regulates gypenoside accumulation via altering the expression of SS and SE in G. pentaphyllum.
