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role of the normal gut microbiota 被引量:222
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作者 sai Manasa Jandhyala Rupjyoti Talukdar +3 位作者 Chivkula Subramanyam Harish Vuyyuru Mitnala Sasikala D Nageshwar Reddy 《World Journal of Gastroenterology》 SCIE CAS 2015年第29期8787-8803,共17页
Relation between the gut microbiota and human health is being increasingly recognised. It is now well established that a healthy gut flora is largely responsible for overall health of the host. The normal human gut mi... Relation between the gut microbiota and human health is being increasingly recognised. It is now well established that a healthy gut flora is largely responsible for overall health of the host. The normal human gut microbiota comprises of two major phyla, namely Bacteroidetes and Firmicutes. Though the gut microbiota in an infant appears haphazard, it starts resembling the adult flora by the age of 3 years. Nevertheless, there exist temporal and spatial variations in the microbial distribution from esophagus to the rectum all along the individual's life span. Developments in genome sequencing technologies and bioinformatics have now enabled scientists to study these microorganisms and their function and microbehost interactions in an elaborate manner both in health and disease. The normal gut microbiota imparts specific function in host nutrient metabolism, xenobiotic and drug metabolism, maintenance of structural integrity of the gut mucosal barrier, immunomodulation, and protection against pathogens. Several factors play a role in shaping the normal gut microbiota. They include(1) the mode of delivery(vaginal or caesarean);(2) diet during infancy(breast milk or formula feeds) and adulthood(vegan based or meat based); and(3) use of antibiotics or antibiotic like molecules that are derived from the environment or the gut commensal community. A major concern of antibiotic use is the long-term alteration of the normal healthy gut microbiota and horizontal transfer of resistance genes that could result in reservoir of organisms with a multidrug resistant gene pool. 展开更多
关键词 NORMAL GUT MICROBIOTA BIOINFORMATICS Health IMMUNOMODULATION METABOLIC function
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Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma 被引量:52
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作者 Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2011年第3期289-294,共6页
BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was und... BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. 展开更多
关键词 hepatocellular carcinoma GLYPICAN-3 IMMUNOHISTOCHEMISTRY nested RT-PCR
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Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice 被引量:37
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作者 Zhengyin Xu Xiameng Xu +9 位作者 Qiang Gong Ziyang Li Ying Li sai Wang Yangyang Yang Wenxiu Ma Longyu Liu Bo Zhu Lifang Zou Gongyou Chen 《Molecular Plant》 SCIE CAS CSCD 2019年第11期1434-1446,共13页
Xanthomonas oryzae pv.oryzae(Xoo),the causal agent of bacterial blight of rice,employs the transcription activator-like effectors(TALEs)to induce the expression of the OsSWEET family of putative sugar transporter gene... Xanthomonas oryzae pv.oryzae(Xoo),the causal agent of bacterial blight of rice,employs the transcription activator-like effectors(TALEs)to induce the expression of the OsSWEET family of putative sugar transporter genes,which function in conferring disease susceptibility(S)in rice plants.To engineer broadspectrum bacterial blight resistance,we used CRISPR/Cas9-mediated gene editing to disrupt the TALEbinding elements(EBEs)of two S genes,OsSWEETH and OsSWEET14,in rice cv.Kitaake,which harbors the recessive resistance allele of Xa25/OsSWEET13.The engineered rice line MS14K exhibited broadspectrum resistance to most Xoo strains with a few exceptions,suggesting that the compatible strains may contain new TALEs.We identified two PthXo2-like TALEs,Tal5LN18 and Tal7PX061,as major virulence factors in the compatible Xoo strains LN18 and PX061,respectively,and found that Xoo encodes at least five types of PthXo2-like effectors.Given that PthXo2/PthXo2.1 target OsSlVEETf3 for transcriptional activation,the genomes of 3000 rice varieties were analyzed for EBE variationsin the OsSWEET13 promoter,and 10Xa25-like haplotypes were identified.We found that Tal5LN18 and Tal7PX〇6i bind slightly different EBE sequences in the OsSWEET13 promoter to activate its expression.CRISPR/Cas9 technology was then used to generate InDels in the EBE of the OsSWEET13 promoter in MS14K to creat a new germplasm with three edited OsSWEET EBEs and broad-spectrum resistance against all Xoo strains tested.Collectively,our findings illustrate how to disarm TALE-S co-evolved loci to generate broad-spectrum resistance through the loss of effector-triggered susceptibility in plants. 展开更多
关键词 Xanthomonas oryzae pv.oryzae TALE susceptible gene ETS RLS
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Delayed hepatocarcinogenesis through antiangiogenic intervention in the nuclear factor-kappa B activation pathway in rats 被引量:31
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作者 Dong, Zhi-Zhen Yao, Deng-Fu +7 位作者 Wu, Wei Yao, Min Yu, Hong-Bo Shen, Jun-Jun Qiu, Li-Wei Yao, Ning-Hua sai, Wen-Li Yang, Jun-Ling 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2010年第2期169-174,共6页
BACKGROUND: The active form of nuclear factor-kappa B (NF-kappa B) is involved in the initiation, generation, and development of hepatocellular carcinoma (HCC), and is up-regulated in inflammation-associated malignanc... BACKGROUND: The active form of nuclear factor-kappa B (NF-kappa B) is involved in the initiation, generation, and development of hepatocellular carcinoma (HCC), and is up-regulated in inflammation-associated malignancies. We investigated the dynamic expression of NF-kappa B and its influences on the occurrence of HCC through antiangiogenic (thalidomide) intervention in NF-kappa B activation. METHODS : Hepatoma models were induced with 2-fluorenylacetamide (2-FAA, 0.05%) in male Sprague-Dawley rats, and thalidomide (100 mg/kg body weight) was administered intragastrically to intervene in NF-kappa B activation. The pathological changes in the liver of sacrificed rats were assessed after hematoxylin and eosin staining. NF-kappa B mRNA was amplified by RT-nested PCR. The alterations of NF-kappa B and vascular endothelial growth factor (VEGF) expression were analyzed by enzyme-linked immunosorbent assay, immunohistochemistry, and Western blotting. RESULTS: Rat hepatocytes showed denatured, precancerous, and cancerous stages in hepatocarcinogenesis, with an increasing tendency of hepatic NF-kappa B, NF-kappa B mRNA, and VEGF expression, and their values in the HCC group were higher than those in controls (P<0.001). In the thalidomide-treated group, the morphologic changes generated only punctiform denaturation and necrosis at the early or middle stages, and nodular hyperplasia or a little atypical hyperplasia at the final stages, with the expression of NF-kappa B (chi(2)=9.93, P<0.001) and VEGF (chi(2)=8.024, P<0.001) lower than that in the 2-FAA group. CONCLUSION: NF-kappa B is overexpressed in hepatocarcinogenesis and antiangiogenic treatment down-regulates the expression of NF-kappa B and VEGF, and delays the occurrence of HCC. (Hepatobiliary Pancreat Dis Int 2010; 9: 169-174) 展开更多
关键词 hepatocellular carcinoma nuclear factor-kappa B vascular endothelial growth factor INTERVENTION dynamic expression
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Quantitative analysis of hepatic hypoxia-inducible factor-1α and its abnormal gene expression during the formation of hepatocellular carcinoma 被引量:28
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作者 Deng-Fu Yao,Hua Jiang,Min Yao,Yue-Ming Li,Wen-Jing Gu,Yu-Cheng Shen, Li-Wei Qiu,Wei Wu,Xin-Hua Wu and Wen-Li sai Research Center of Clinical Molecular Biology,Department of Laboratory Science,and Department of Oncology,Affiliated Hospital of Nantong University,Nantong 226001, China Third Department of Medicine,Jiaonan People’s Hospital,Jiaonan 266400,China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2009年第4期407-413,共7页
BACKGROUND:Hepatic hypoxia-inducible factor-1(HIF-1) is activated in the progression of hepatocellular carcinoma (HCC).This study aimed to investigate the dynamic alterations of HIF-1αand its gene expression so as to... BACKGROUND:Hepatic hypoxia-inducible factor-1(HIF-1) is activated in the progression of hepatocellular carcinoma (HCC).This study aimed to investigate the dynamic alterations of HIF-1αand its gene expression so as to explore the relationship between HIF-1αexpression and hepatocarcinogenesis at the early stage of HCC. METHODS:A hepatoma model was made with 2-fluorenyl- acetamide(2-FAA)in male Sprague-Dawley rats.Morphological changes of rat hepatocytes were assessed pathologically (HE staining).The dynamic expression of hepatic and circulating HIF-1αwas quantitatively analyzed by ELISA. The gene fragments of hepatic HIF-1αmRNA were amplified by RT-PCR and confirmed by sequencing.The cellular distribution of hepatic HIF-1αexpression was confirmed by immunohistochemistry. RESULTS:Histological examination confirmed granulelike degeneration to atypical hyperplasia and HCC development in rat hepatocytes and progressive increases in the levels of hepatic and circulating HIF-1αand its gene expression during the course.The levels of HIF-1α expression in the liver and blood of rats with hepatoma were significantly higher than those in normal ratsand those with degeneration.Immunohistochemical analysis confirmed the positive expression and hepatocyte distribution of HIF-1αin the development of rat hepatoma. A positive relationship was found between HIF-1α expression in the liver and blood(P<0.01). CONCLUSIONS:The above observations support the hypothesis that the overexpression of HIF-1αand its gene are closely associated with the malignant transformation of hepatocytes and play an important role at the stage of hepatocarcinogenesis. 展开更多
关键词 hepatocellular carcinoma hypoxia inducible factor-1α early expression dynamic alteration
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The role of Epstein-Barr virus infection in the pathogenesis of nasopharyngeal carcinoma 被引量:26
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作者 Chi Man +3 位作者 Tsang sai Wah Tsao 《Virologica Sinica》 SCIE CAS CSCD 2015年第2期107-121,共15页
Nasopharyngeal carcinoma(NPC) is closely associated with Epstein-Barr virus(EBV) infection. EBV episomes are detected in almost all NPC cells. The role of EBV in NPC pathogenesis has long been postulated but remains e... Nasopharyngeal carcinoma(NPC) is closely associated with Epstein-Barr virus(EBV) infection. EBV episomes are detected in almost all NPC cells. The role of EBV in NPC pathogenesis has long been postulated but remains enigmatic. In contrast to infection of B lymphocytes, EBV infection does not directly transform nasopharyngeal epithelial cells into proliferative clones with malignant potential. EBV infection of normal pharyngeal epithelial cells is predominantly lytic in nature. Genetic alterations in premalignant nasopharyngeal epithelium, in combination with inflammatory stimulation in the nasopharyngeal mucosa, presumably play essential roles in the establishment of a latent EBV infection in infected nasopharyngeal epithelial cells during the early development of NPC. Establishment of latent EBV infection in premalignant nasopharyngeal epithelial cells and expression of latent viral genes, including the BART transcripts and BART-encoded micro RNAs, are crucial features of NPC. Expression of EBV genes may drive further malignant transformation of premalignant nasopharyngeal epithelial cells into cancer cells. The difficulties involved in the establishment of NPC cell lines and the progressive loss of EBV epsiomes in NPC cells propagated in culture strongly implicate the contribution of host stromal components to the growth of NPC cells in vivo and maintenance of EBV in infected NPC cells. Defining the growth advantages of EBV-infected NPC cells in vivo will lead to a better understanding of the contribution of EBV infection in NPC pathogenesis, and may lead to the identification of novel therapeutic targets for NPC treatment. 展开更多
关键词 EPSTEIN-BARR virus(EBV) NASOPHARYNGEAL carcinoma LATENT infection PATHOGENESIS inflammation
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A coordinated dispatch method with pumped-storage and battery-storage for compensating the variation of wind power 被引量:24
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作者 Jinghua Li sai Wang +1 位作者 Liu Ye Jiakun Fang 《Protection and Control of Modern Power Systems》 2018年第1期21-34,共14页
Growing penetration of wind power challenges to power system security,since the conventional generators may not have sufficient capacity to compensate wind power fluctuation plus the reverse peak regulation.In this pa... Growing penetration of wind power challenges to power system security,since the conventional generators may not have sufficient capacity to compensate wind power fluctuation plus the reverse peak regulation.In this paper,the high-capacity pumped-storage and fast-response battery-storage are coordinated to compensate the variation of both wind power and load,aiming at shifting peak load,responding to wind power ramping,reducing the curtailment of wind and stabilizing the output of thermal units.A practical framework is designed for optimizing the operation of the hybrid system consisting of the wind,pumped-storage,and battery storage,which can take full advantages of pumped-storage and battery-storage.The detailed mathematical formulations of the pumpedstorage and battery-storage are built.Three cases are studied to demonstrate the advantages of the proposed coordination method. 展开更多
关键词 Pumped-storage Battery-storage Hybrid energy system Economic dispatch Renewable energy
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Genome Analysis of the Ancient Tracheophyte Selaginella tama#scina Reveals Evolutionary Features Relevant to the Acquisition of Desiccation Tolerance 被引量:22
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作者 Zhichao Xu Tianyi Xin +12 位作者 Dorothea Bartels Ying Li Wei Gu Hui Yao sai Liu Haoying Yu Xiangdong Pu Jianguo Zhou Jiang Xu Caicai xi Hetian Lei Jingyuan song Shilin Chen 《Molecular Plant》 SCIE CAS CSCD 2018年第7期983-994,共12页
Resurrection plants, which are the "gifts" of natural evolution, are ideal models for studying the genetic basis of plant desiccation tolerance. Here, we report a high-quality genome assembly of 301 Mb for the diplo... Resurrection plants, which are the "gifts" of natural evolution, are ideal models for studying the genetic basis of plant desiccation tolerance. Here, we report a high-quality genome assembly of 301 Mb for the diploid spike moss Selaginella tamariscina, a primitive vascular resurrection plant. We predicated 27 761 protein-coding genes from the assembled S. tarnariscina genome, 11.38% (2363) of which showed signifi- cant expression changes in response to desiccation. Approximately 60.58% of the S. tamariscina genome was annotated as repetitive DNA, which is an almost 2-fold increase of that in the genome of desiccation- sensitive Selaginella moellendorffii. Genomic and transcriptomic analyses highlight the unique evolution and complex regulations of the desiccation response in S. tamariscina, including species-specific expan- sion of the oleosin and pentatricopeptide repeat gene families, unique genes and pathways for reactive oxygen species generation and scavenging, and enhanced abscisic acid (ABA) biosynthesis and potentially distinct regulation of ABA signaling and response. Comparative analysis of chloroplast genomes of several Selaginella species revealed a unique structural rearrangement and the complete loss of chloroplast NAD(P) H dehydrogenase (NDH) genes in S. tamariscina, suggesting a link between the absence of the NDH com- plex and desiccation tolerance. Taken together, our comparative genomic and transcriptomic analyses reveal common and species-specific desiccation tolerance strategies in S. tamariscina, providing signifi- cant insights into the desiccation tolerance mechanism and the evolution of resurrection plants. 展开更多
关键词 resurrection plant Selaginella tamariscina herbgenomics evolution synthetic biology
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Essential oil and aromatic plants as feed additives in non-ruminant nutrition:a review 被引量:24
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作者 Zhikai Zeng sai Zhang +1 位作者 Hongliang Wang Xiangshu Piao 《Journal of Animal Science and Biotechnology》 CAS CSCD 2016年第1期10-19,共10页
This paper summarizes the current knowledge regarding the possible modes of action and nutritional factors involved in the use of essential oils (EOs) for swine and poultry. EOs have recently attracted increased int... This paper summarizes the current knowledge regarding the possible modes of action and nutritional factors involved in the use of essential oils (EOs) for swine and poultry. EOs have recently attracted increased interest as feed additives to be fed to swine and poultry, possibly replacing the use of antibiotic growth promoters which have been prohibited in the European Union since 2006. In general, EOs enhance the production of digestive secretions and nutrient absorption, reduce pathogenic stress in the gut, exert antioxidant properties and reinforce the animal's immune status, which help to explain the enhanced performance observed in swine and poultry. However, the mechanisms involved in causing this growth promotion are far from being elucidated, since data on the complex gut ecosystem, gut function, in vivo oxidative status and immune system are still lacking. In addition, limited information is available regarding the interaction between EOs and feed ingredients or other feed additives (especially pro- or prebiotics and organic acids). This knowledge may help feed formulators to better utilize EOs when they formulate diets for poultry and swine. 展开更多
关键词 ANTIMICROBIAL ANTIOXIDANT Essential oils Feed additives Growth promoter Gut function IMMUNITY
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Simultaneous Determination of Melamine, Ammelide, Ammeline, and Cyanuric Acid in Milk and Milk Products by Gas Chromatography-tandem Mass Spectrometry 被引量:22
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作者 HONG MIAO sai FAN +5 位作者 YONG-NING WU LEI ZHANG PING-PING ZHOU JING-GUANG LI HUI-JING CHEN AND YUN-FENG ZHAO 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2009年第2期87-94,共8页
Objective To develop an analytical method for simultaneously qualitative and quantitative determination of melamine and triazine-related by-products including ammelide, ammeline, and cyanuric acid in milk and milk pro... Objective To develop an analytical method for simultaneously qualitative and quantitative determination of melamine and triazine-related by-products including ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography- tandem mass spectrometry (GC-MS/MS). Methods Melamine and triazine-related by-products namely ammelide, ammeline and cyanuric acid in the samples were extracted in a solvent mixture of diethylamine, water, and acetonitrile (10:40:50, V/V/V). After centrifugation, an aliquot of the supernatant was evaporated to dryness under a gentle stream of nitrogen gas, and then melamine and triazine-related by-products were derivatized using BSTFA with 1% TMCS. The derivatives of melamine and its analogues were determined by gas chromatography/tandem mass spectrometry using multiple reactional monitoring (MRM) with 2, 6-Diamino-4-chloropyrimidine (DACP) being used as an internal standard. Results The linear detectable ranges were from 0.004 mg/kg to 1.6 mg/kg for melamine, ammelide, ammeline, and cyanuric acid with a correlation coefficient no less than 0.999. The recovery rates of the tour compounds in spiked blank milk powder at concentrations 0.5, 1, 2 mg/kg were between 61.4%-117.2%, and the relative standard deviation was no more than 11.5% (n=6). The detection limits of melamine, ammelide, ammeline and cyanuric acid in milk powder were 0.002 mg/kg with a ratio of signal to noise of 3. Conclusion This GC-MS/MS method for simultaneous determination of melamine, ammelide, ammeline, and cyanuric acid in milk and milk products is sensitive and specific. 展开更多
关键词 MELAMINE Ammelide Ammeline Cyanuric acid GC-MS/MS Milk products
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煤与瓦斯突出危险精准辨识理论方法与技术探索 被引量:22
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作者 舒龙勇 朱南南 +2 位作者 陈结 安赛 张慧杰 《煤炭学报》 EI CAS CSCD 北大核心 2020年第5期1614-1625,共12页
针对我国煤与瓦斯突出频发、突出事故呈现新特征、突出预测方法和指标体系有待完善等现实问题,为了更好地指导突出预测与防治工作,以煤与瓦斯突出关键结构体致灾机理为指导,基于突出发生位置必须具备特殊的地质结构环境这一基本认识,提... 针对我国煤与瓦斯突出频发、突出事故呈现新特征、突出预测方法和指标体系有待完善等现实问题,为了更好地指导突出预测与防治工作,以煤与瓦斯突出关键结构体致灾机理为指导,基于突出发生位置必须具备特殊的地质结构环境这一基本认识,提出了煤与瓦斯突出危险“层层递进-精准辨识”的理论方法,具体包括3个层次:采用物探和钻探相结合的手段,超前探测采掘工作面周围存在的异常地质结构;采用微震和瓦斯涌出实时监测相结合的方法,动态分析采掘扰动条件下采掘工作面前方煤体结构、地应力和瓦斯大小变化特征;采用随钻测定相关特征参数和预测指标的方法,进一步验证超前探测和实时监测的辨识结果。探索性工程试验表明:“超前探测地质结构异常-实时监测煤体突出危险性-随钻测定煤层瓦斯大小”相结合的突出危险辨识技术能够综合反映采掘工作面周围地质结构、煤体结构、地应力和瓦斯大小变化特征,促进了突出预测工作由点预测向面预测、由间断式向连续式、由接触式向接触-非接触式相结合的转变。通过进一步发展物探和钻探相结合的精细探测技术,引入大数据分析和机器学习算法等训练综合判识模型,开发以“超前探测异常地质结构,实时监测采掘工作面微震信号和瓦斯涌出时序变化特征,随钻测定各种特征参数和预测指标”为核心的综合预警系统,实现突出灾害多元信息综合监测与智能预警,更好地服务于煤与瓦斯突出防治工作。 展开更多
关键词 煤与瓦斯突出 突出预测预报 超前探测 实时监测 随钻测定
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Review of collapse triggering mechanism of collapsible soils due towetting 被引量:22
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作者 Ping Li sai Vanapalli Tonglu Li 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2016年第2期256-274,共19页
Loess soil deposits are widely distributed in arid and semi-arid regions and constitute about 10% of land area of the world.These soils typically have a loose honeycomb-type meta-stable structure that is susceptible t... Loess soil deposits are widely distributed in arid and semi-arid regions and constitute about 10% of land area of the world.These soils typically have a loose honeycomb-type meta-stable structure that is susceptible to a large reduction in total volume or collapse upon wetting.Collapse characteristics contribute to various problems to infrastructures that are constructed on loess soils.For this reason,collapse triggering mechanism for loess soils has been of significant interest for researchers and practitioners all over the world.This paper aims at providing a state-of-the-art review on collapse mechanism with special reference to loess soil deposits.The collapse mechanism studies are summarized under three different categories,i.e.traditional approaches,microstructure approach,and soil mechanics-based approaches.The traditional and microstructure approaches for interpreting the collapse behavior are comprehensively summarized and critically reviewed based on the experimental results from the literature.The soil mechanics-based approaches proposed based on the experimental results of both compacted soils and natural loess soils are reviewed highlighting their strengths and limitations for estimating the collapse behavior.Simpler soil mechanics-based approaches with less parameters or parameters that are easy-to-determine from conventional tests are suggested for future research to better understand the collapse behavior of natural loess soils.Such studies would be more valuable for use in conventional geotechnical engineering practice applications. 展开更多
关键词 Collapse mechanism Microstructure Constitutive relationships Compacted soils Natural loess soils Elastoplastic models Yield surface Structural strength
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肺癌外科手术进展 被引量:21
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作者 Mark W.Hennon sai Yendamuri +1 位作者 丁燕 南娟 《中国肺癌杂志》 CAS 北大核心 2013年第3期I0004-I0007,共4页
近年来微创技术在各期肺癌的检测、诊断和治疗中的应用出现爆发。这些技术的应用提高了手术的风险-收益比,并且使考虑肺癌手术治疗的患者更易接受手术。同时它们为晚期肺癌患者综合治疗的实施提供了便利。该综述总结了代表肺癌胸外科手... 近年来微创技术在各期肺癌的检测、诊断和治疗中的应用出现爆发。这些技术的应用提高了手术的风险-收益比,并且使考虑肺癌手术治疗的患者更易接受手术。同时它们为晚期肺癌患者综合治疗的实施提供了便利。该综述总结了代表肺癌胸外科手术前沿的现有外科技术。 展开更多
关键词 肺癌 预后 胸腔镜
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Values of circulating GPC-3 mRNA and alpha-fetoprotein in detecting patients with hepatocellular carcinoma 被引量:19
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作者 Min Yao Deng-Fu Yao +8 位作者 Yin-Zhu Bian Wei Wu Xiao-Di Yan Dan-Dan Yu Li-Wei Qiu Jun-Ling Yang Hai-Jian Zhang Wen-Li sai Jie Chen 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2013年第2期171-179,共9页
BACKGROUND: The prognosis of hepatocellular carcinoma (HCC) is poor and its early diagnosis is of the utmost importance. This study aimed to investigate the values of glypican-3 (GPC-3) expression in the liver and ser... BACKGROUND: The prognosis of hepatocellular carcinoma (HCC) is poor and its early diagnosis is of the utmost importance. This study aimed to investigate the values of glypican-3 (GPC-3) expression in the liver and sera and its gene transcription for diagnosis and monitoring of metastasis of HCC. METHODS: Liver GPC-3 was analyzed in HCC tissues from 36 patients by immunohistochemistry and Western blotting. GPC-3 mRNA from circulating peripheral blood mononuclear cells from 123 HCC patients or 246 patients with other diseases or 36 HCC tissues was amplified by RT-PCR, quantitative realtime PCR, and confirmed by DNA sequencing. Circulating GPC-3 level was detected by ELISA. RESULTS: The increasing expression of GPC-3 was observed from non-cancerous to cancerous tissues, with brown granule-like staining localized in tumor parts of atypical hyperplasia and HCC formation. The positive rate of GPC-3 was 80.6% in HCC, 41.7% in their paracancerous tissues, and none in distal cancerous tissues (P【0.001), with no significant difference in differentiation grade and tumor number except for size (Z=2.941, P=0.003). Serum GPC-3 was detected only in HCC (52.8%) and significant difference was found between GPC-3 and tumor size (χ2 =6.318, P=0.012) or HBV infection (χ2 =23.362, P【0.001). Circulating GPC-3 mRNA was detected in 70.7% of HCC tissues, with relation to TNM stage, periportal cancerous embolus, and extra-hepatic metastasis (P【0.001). The combination ofcirculating GPC-3, GPC-3 mRNA and alpha-fetoprotein is of complementary value for HCC diagnosis (94.3%). CONCLUSION: Both GPC-3 overexpression and GPC-3 mRNA abnormality could be used as markers for the diagnosis of HCC and monitoring its metastasis. 展开更多
关键词 hepatocellular carcinoma GLYPICAN-3 IMMUNOHISTOCHEMISTRY quantitative real-time PCR gene expression sequencing
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PM_(2.5)-bound polycyclic aromatic hydrocarbons(PAHs) in Beijing:Seasonal variations,sources,and risk assessment 被引量:19
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作者 Baihuan Feng Lijuan Li +8 位作者 Hongbing Xu Tong Wang Rongshan Wu Jie Chen Yi Zhang Shuo Liu Steven sai Hang Ho Junji Cao Wei Huang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2019年第3期11-19,共9页
Polycyclic aromatic hydrocarbons(PAHs) have been of health concern due to its carcinogenesis and mutagenesis. In this study, we aimed to assess the variations, sources, and lifetime excessive cancer risk(ECR) attribut... Polycyclic aromatic hydrocarbons(PAHs) have been of health concern due to its carcinogenesis and mutagenesis. In this study, we aimed to assess the variations, sources, and lifetime excessive cancer risk(ECR) attributable to PAHs bound to ambient particulate matters with aerodynamic diameter less than 2.5 μm(PM_(2.5)) in metropolitan Beijing, China. We collected24-hour integrated PM_(2.5) samples on daily basis between November 2014 and June 2015 across both central heating(cold months) and non-heating(warm months) seasons, and further analyzed the PAH components in these daily PM_(2.5) samples. Our results showed that total concentrations of PM_(2.5)-bound PAHs varied between(88.6 ± 75.4) ng/m^3 in the cold months and(11.0 ± 5.9) ng/m^3 in the warm months. Benzo[a]pyrene(Ba P), the carcinogenic marker of PAHs,averaged at 5.7 and 0.4 ng/m^3 in the cold and warm months, respectively. Source apportionment analyses illustrated that gasoline, biomass burning, diesel, coal combustion and cooking were the major contributors, accounting for 12.9%, 17.8%, 24.7%, 24.3% and 6.4% of PM_(2.5)-bound PAHs, respectively. The BaP equivalent lifetime ECR from inhalation of PM_(2.5)-bound PAHs was 16.2 cases per million habitants. Our results suggested that ambient particulate reduction from energy reconstruction and adaption of clean fuels would result in reductions PM_(2.5)-bound PAHs and its associated cancer risks. However, as only particulate phased PAHs was analyzed in the present study, the concentration of ambient PAHs could be underestimated. 展开更多
关键词 PM2.5 PAHS SEASONAL variation SOURCES Cancer risk assessment
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One-year clinical study of NeuroR egen scaffold implantation following scar resection in complete chronic spinal cord injury patients 被引量:18
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作者 Zhifeng Xiao Fengwu Tang +15 位作者 Jiaguang Tang Huilin Yang Yannan Zhao Bing Chen Sufang Han Nuo Wang Xing Li Shixiang Cheng Guang Han Changyu Zhao Xiaoxiong Yang Yumei Chen Qin Shi Shuxun Hou sai Zhang Jianwu Dai 《Science China(Life Sciences)》 SCIE CAS CSCD 2016年第7期647-655,共9页
The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, Neuro Regen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemic... The objective of this clinical study was to assess the safety and feasibility of the collagen scaffold, Neuro Regen scaffold, one year after scar tissue resection and implantation. Scar tissue is a physical and chemical barrier that prevents neural regeneration. However, identification of scar tissue is still a major challenge. In this study, the nerve electrophysiology method was used to distinguish scar tissue from normal neural tissue, and then different lengths of scars ranging from 0.5–4.5 cm were surgically resected in five complete chronic spinal cord injury(SCI) patients. The NeuroR egen scaffold along with autologous bone marrow mononuclear cells(BMMCs), which have been proven to promote neural regeneration and SCI recovery in animal models, were transplanted into the gap in the spinal cord following scar tissue resection. No obvious adverse effects related to scar resection or Neuro Regen scaffold transplantation were observed immediately after surgery or at the 12-month follow-up. In addition, patients showed partially autonomic nervous function improvement, and the recovery of somatosensory evoked potentials(SSEP) from the lower limbs was also detected. The results indicate that scar resection and Neuro Regen scaffold transplantation could be a promising clinical approach to treating SCI. 展开更多
关键词 NeuroRegen scaffold chronic spinal cord injury scar resection collagen scaffold transplantation bone marrow mon-onuclear cells tissue regeneration
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Operational optimization of a building-level integrated energy system considering additional potential benefits of energy storage 被引量:18
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作者 sai Liu Cheng Zhou +4 位作者 Haomin Guo Qingxin Shi Tiancheng E.Song Ian Schomer Yu Liu 《Protection and Control of Modern Power Systems》 2021年第1期55-64,共10页
As a key component of an integrated energy system(IES),energy storage can effectively alleviate the problem of the times between energy production and consumption.Exploiting the benefits of energy storage can improve ... As a key component of an integrated energy system(IES),energy storage can effectively alleviate the problem of the times between energy production and consumption.Exploiting the benefits of energy storage can improve the competitiveness of multi-energy systems.This paper proposes a method for day-ahead operation optimization of a building-level integrated energy system(BIES)considering additional potential benefits of energy storage.Based on the characteristics of peak-shaving and valley-filling of energy storage,and further consideration of the changes in the system’s load and real-time electricity price,a model of additional potential benefits of energy storage is developed.Aiming at the lowest total operating cost,a bi-level optimal operational model for day-ahead operation of BIES is developed.A case analysis of different dispatch strategies verifies that the addition of the proposed battery scheduling strategy improves economic operation.The results demonstrate that the model can exploit energy storage’s potential,further optimize the power output of BIES and reduce the economic cost. 展开更多
关键词 Building-level integrated energy system Energy storage Additional potential benefits Bi-level optimization
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Comprehensive Utilization of Paigeite Ore Using Iron Nugget Making Process 被引量:18
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作者 DING Yin-gui WANG Jing-song +2 位作者 WANG Guang MA sai XUE Qing-guo 《Journal of Iron and Steel Research International》 SCIE CAS CSCD 2012年第6期9-13,共5页
The feasibility of paigeite ore treatment with iron nugget making process is proved. The isothermal reduc- tion experiments at laboratory scale were carried out, using carbon bearing pellets which were made of boron c... The feasibility of paigeite ore treatment with iron nugget making process is proved. The isothermal reduc- tion experiments at laboratory scale were carried out, using carbon bearing pellets which were made of boron contai- ning iron concentrate and pulverized coal mainly, from 1 623 to 1 723 K with different heating time. The results indi- cated that iron nugget making process depends mainly on heating time and temperature. And the iron nugget and slag can separate in a clean manner at 1 673 K for 15 min. For the iron nugget, the C content is 3.57% (mass percent) and B is 0. 065~ (mass percent). The B203 content of slag is 20.01~/00, and the boron was concentrated into one phase which is identified as suanite (Mg2BaOs) during the solidification. With an extraction ratio of 80~ under the atmospheric conditions, the activity of boron in slag is good. The boron-rich slag can be used to extract boric or boric acid and alleviate the shortage of boron resource. Through series of calculation and analysis related, it can be conclu- ded that the recovery ratio of Fe and boron are about 98% and 97% respectively. The results show that this method is feasible and effective on the utilization of paigeite ore. 展开更多
关键词 boron containing iron concentrate carbon bearing pellets iron nugget boron-rich slag
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Neuroprotective effects of bloodletting at Jing points combined with mild induced hypothermia in acute severe traumatic brain injury 被引量:17
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作者 Yue Tu Xiao-mei Miao +4 位作者 Tai-long Yi Xu-yi Chen Hong-tao Sun Shi-xiang Cheng sai Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第6期931-936,共6页
Bloodletting at Jing points has been used to treat coma in traditional Chinese medicine. Mild induced hypothermia has also been shown to have neuroprotective effects. However, the therapeutic effects of bloodletting a... Bloodletting at Jing points has been used to treat coma in traditional Chinese medicine. Mild induced hypothermia has also been shown to have neuroprotective effects. However, the therapeutic effects of bloodletting at Jing points and mild induced hypothermia alone are limited. Therefore, we investigated whether combined treatment might have clinical effectiveness for the treatment of acute severe traumatic brain injury. Using a rat model of traumatic brain injury, combined treatment substantially alleviated cerebral edema and bloodbrain barrier dysfunction. Furthermore, neurological function was ameliorated, and cellular necrosis and the inflammatory response were lessened. These findings suggest that the combined effects of bloodletting at Jing points(20 μL, twice a day, for 2 days) and mild induced hypothermia(6 hours) are better than their individual effects alone. Their combined application may have marked neuroprotective effects in the clinical treatment of acute severe traumatic brain injury. 展开更多
关键词 traumatic alone edema neurological minutes hematoma hematoxylin olfactory neuronal twice
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Three-dimensional bioprinting collagen/silk fibroin scaffold combined with neural stem cells promotes nerve regeneration after spinal cord injury 被引量:16
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作者 Ji-Peng Jiang Xiao-Yin Liu +9 位作者 Fei Zhao Xiang Zhu Xiao-Yin Li Xue-Gang Niu Zi-Tong Yao Chen Dai Hui-You Xu Ke Ma Xu-Yi Chen sai Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第5期959-968,共10页
Many studies have shown that bio-scaffolds have important value for promoting axonal regeneration of injured spinal cord.Indeed,cell transplantation and bio-scaffold implantation are considered to be effective methods... Many studies have shown that bio-scaffolds have important value for promoting axonal regeneration of injured spinal cord.Indeed,cell transplantation and bio-scaffold implantation are considered to be effective methods for neural regeneration.This study was designed to fabricate a type of three-dimensional collagen/silk fibroin scaffold (3D-CF) with cavities that simulate the anatomy of normal spinal cord.This scaffold allows cell growth in vitro and in vivo.To observe the effects of combined transplantation of neural stem cells (NSCs) and 3D-CF on the repair of spinal cord injury.Forty Sprague-Dawley rats were divided into four groups: sham (only laminectomy was performed),spinal cord injury (transection injury of T10 spinal cord without any transplantation),3D-CF (3D scaffold was transplanted into the local injured cavity),and 3D-CF + NSCs (3D scaffold co-cultured with NSCs was transplanted into the local injured cavity.Neuroelectrophysiology,imaging,hematoxylin-eosin staining,argentaffin staining,immunofluorescence staining,and western blot assay were performed.Apart from the sham group,neurological scores were significantly higher in the 3D-CF + NSCs group compared with other groups.Moreover,latency of the 3D-CF + NSCs group was significantly reduced,while the amplitude was significantly increased in motor evoked potential tests.The results of magnetic resonance imaging and diffusion tensor imaging showed that both spinal cord continuity and the filling of injury cavity were the best in the 3D-CF + NSCs group.Moreover,regenerative axons were abundant and glial scarring was reduced in the 3D-CF + NSCs group compared with other groups.These results confirm that implantation of 3D-CF combined with NSCs can promote the repair of injured spinal cord.This study was approved by the Institutional Animal Care and Use Committee of People’s Armed Police Force Medical Center in 2017 (approval No.2017-0007.2). 展开更多
关键词 3D BIOPRINTING COLLAGEN diffusion tensor IMAGING functional recovery magnetic resonance IMAGING nerve REGENERATION NEURAL REGENERATION NEURAL stem cell SCAFFOLD silk fibroin spinal cord injury
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