<div style="text-align:justify;"> Pomegranate flowers as row materials were used for extraction of polysaccharides by water-extraction and alcohol-precipitation method. After purification, the physical...<div style="text-align:justify;"> Pomegranate flowers as row materials were used for extraction of polysaccharides by water-extraction and alcohol-precipitation method. After purification, the physical and chemical properties, structure, monosaccharide composition and molecular weight were studied. The results showed that the polysaccharides from pomegranate flowers mainly contained two kinds of water soluble acidic polysaccharides, and monosaccharide composition were arabinose and galactose, both contained hydroxyl, carboxyl, amino, hydroxyl radical, sulfate, beta glycosidic bond and alpha glycosidic bond structure. The molecular weight of PP1 and PP2 were 6.16 × 104 (±6.6%) and 9.01 × 104 (±3.2%), respectively. The results of this study laid the foundation for further development and application of polysaccharides from pomegranate flowers. </div>展开更多
本研究采用16S r DNA PCR-RFLP、IGS PCR-RFLP和16S r DNA基因测序和系统发育分析等分子鉴定方法,对分离的汝州白三叶草共生根瘤菌进行了多样性研究。结果表明,21株供试菌株仅产生了一种16S r DNA酶切图谱组合类型,通过IGS PCR-RFLP又...本研究采用16S r DNA PCR-RFLP、IGS PCR-RFLP和16S r DNA基因测序和系统发育分析等分子鉴定方法,对分离的汝州白三叶草共生根瘤菌进行了多样性研究。结果表明,21株供试菌株仅产生了一种16S r DNA酶切图谱组合类型,通过IGS PCR-RFLP又把所有的供试菌株分为了6种酶切图谱类型。经过对代表菌株的16S r DNA基因测序及系统发育分析,6个代表菌株之间具有99.9%~100%的相似性,且供试菌株与根瘤菌属的已知种群Rhizobium anhuiense和Rhizobium leguminosarum等也具有99.9%~100%的相似性,因此,分离自汝州的白三叶草根瘤菌属于根瘤菌属(Rhizobium)。展开更多
文摘<div style="text-align:justify;"> Pomegranate flowers as row materials were used for extraction of polysaccharides by water-extraction and alcohol-precipitation method. After purification, the physical and chemical properties, structure, monosaccharide composition and molecular weight were studied. The results showed that the polysaccharides from pomegranate flowers mainly contained two kinds of water soluble acidic polysaccharides, and monosaccharide composition were arabinose and galactose, both contained hydroxyl, carboxyl, amino, hydroxyl radical, sulfate, beta glycosidic bond and alpha glycosidic bond structure. The molecular weight of PP1 and PP2 were 6.16 × 104 (±6.6%) and 9.01 × 104 (±3.2%), respectively. The results of this study laid the foundation for further development and application of polysaccharides from pomegranate flowers. </div>
文摘本研究采用16S r DNA PCR-RFLP、IGS PCR-RFLP和16S r DNA基因测序和系统发育分析等分子鉴定方法,对分离的汝州白三叶草共生根瘤菌进行了多样性研究。结果表明,21株供试菌株仅产生了一种16S r DNA酶切图谱组合类型,通过IGS PCR-RFLP又把所有的供试菌株分为了6种酶切图谱类型。经过对代表菌株的16S r DNA基因测序及系统发育分析,6个代表菌株之间具有99.9%~100%的相似性,且供试菌株与根瘤菌属的已知种群Rhizobium anhuiense和Rhizobium leguminosarum等也具有99.9%~100%的相似性,因此,分离自汝州的白三叶草根瘤菌属于根瘤菌属(Rhizobium)。
