The fabrication of heterojunction catalysts is an effective strategy to enhance charge separation efficiency,thereby boosting the performance of photocatalysts.In this study,BiO_(2-x)nanosheets were synthesized throug...The fabrication of heterojunction catalysts is an effective strategy to enhance charge separation efficiency,thereby boosting the performance of photocatalysts.In this study,BiO_(2-x)nanosheets were synthesized through a hydrothermal process and loaded onto NaNbO_(3) microcube to construct a series of BiO_(2-x)/NaNbO_(3) heterojunctions for photocatalytic N_(2) fixation.Results indicated that 2.5%BiO_(2-x)/NaNbO_(3) had the highest photocatalytic performance.The NH_(3) production rate under simulated solar light reached 406.4μmol·L^(-1)·g^(-1)·h^(-1),which reaches 2.6 and 3.8 times that of NaNbO_(3) and BiO_(2-x),respectively.BiO_(2-x)nanosheets primarily act as electron trappers to enhance the separation efficiency of charge carriers.The strong interaction between BiO_(2-x)and NaNbO_(3) facilitates the electron migration between them.Meanwhile,the abundant oxygen vacancies in BiO_(2-x)nanosheets may facilitate the adsorption and activation of N_(2),which may be another possible reason of the high photocatalytic activity of the BiO_(2-x)/NaNbO_(3).This study may offer new insights for the development of semiconductor materials in photocatalytic nitrogen fixation.展开更多
Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion therapy.Although increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutroph...Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion therapy.Although increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutrophils in this process remain unclear.Here,we performed single-cell sequencing of cardiac CD45^(+)cells isolated from the murine myocardium subjected to MIRI at six-time points.We identified diverse types of infiltrating immune cells and their dynamic changes during MIRI.Cardiac neutrophils showed the most immediate response and largest changes and featured with functionally heterogeneous subpopulations,including Ccl3^(hi)Neu and Ym-1^(hi)Neu,which were increased at 6 h and 1 d after reperfusion,respectively.Ym-1^(hi)Neu selectively expressed genes with protective effects and was,therefore,identified as a novel specific type of cardiac cell in the injured heart.Further analysis indicated that neutrophils and their subtypes orchestrated subsequent immune responses in the cardiac tissues,especially instructing the response of macrophages.The abundance of Ym-1^(hi)Neu was closely correlated with the therapeutic efficacy of MIRI when neutrophils were specifically targeted by anti-Lymphocyte antigen 6 complex locus G6D(Ly6G)or anti-Intercellular cell adhesion molecule-1(ICAM-1)neutralizing antibodies.In addition,a neutrophil subtype with the same phenotype as Ym-1^(hi)Neu was detected in clinical samples and correlated with prognosis.Ym-1 inhibition exacerbated myocardial injury,whereas Ym-1 supplementation significantly ameliorated injury in MIRI mice,which was attributed to the tilt of Ym-1 on the polarization of macrophages toward the repair phenotype in myocardial tissue.Overall,our findings reveal the antiinflammatory phenotype of Ym-1^(hi)Neu and highlight its critical role in myocardial protection during the early stages of MIRI.展开更多
Sepsis is a lethal condition characterized by multiple organ dysfunction due to disrupted host responses to severe infections.[1]Aff ected patients often have a Sequential Organ Failure Assessment(SOFA)score≥2.[2]Pat...Sepsis is a lethal condition characterized by multiple organ dysfunction due to disrupted host responses to severe infections.[1]Aff ected patients often have a Sequential Organ Failure Assessment(SOFA)score≥2.[2]Patients with a SOFA score<2 and at least one of the following were considered as“suspected sepsis”:(1)quick SOFA(qSOFA)score≥2;(2)SOFA score=1;or(3)National Early Warning Score(NEWS)4-6.[3]Compared with studies on fluid resuscitation in sepsis patients,there are few studies on fluid management in patients with suspected sepsis.Therefore,we conducted a retrospective cohort study to evaluate the relationship between fluid management and disease progression in suspected sepsis patients.展开更多
Dwarfing is a pivotal agronomic trait affecting both yield and quality.Citrus species exhibit substantial variation in plant height,among which internode length is a core element.However,the molecular mechanism govern...Dwarfing is a pivotal agronomic trait affecting both yield and quality.Citrus species exhibit substantial variation in plant height,among which internode length is a core element.However,the molecular mechanism governing internode elongation remains unclear.Here,we unveiled that the transcriptional cascade consisting of B-BOX DOMAIN PROTEIN 22(BBX22)and ELONGATED HYPOCOTYL 5(HY5)finely tunes plant height and internode elongation in citrus.Loss-of-function mutations of BBX22 in an early-flowering citrus(Citrus hindsii“SJG”)promoted internode elongation and reduced pigment accumulation,whereas ectopic expression of BBX22 in SJG,sweet orange(C.sinensis),pomelo(C.maxima)or heterologous expression of BBX22 in tomato(Solanum lycopersicum)significantly decreased internode length.Furthermore,exogenous application of gibberellin A3(GA3)rescued the shortened internode and dwarf phenotype caused by BBX22overexpression.Additional experiments revealed that BBX22 played a dual role in regulation internode elongation and pigmentation in citrus.On the one hand,it directly bound to and activated the expression of HY5,GA metabolism gene(GA2 OXIDASE 8,GA2ox8),carotenoid biosynthesis gene(PHYTOENE SYNTHASE 1,PSY1)and anthocyanin regulatory gene(Ruby1,a MYB DOMAIN PROTEIN).On the other hand,it acted as a cofactor of HY5,enhancing the ability of HY5 to regulate target genes expression.Together,our results reveal the critical role of the transcriptional cascade consisting of BBX22 and HY5 in controlling internode elongation and pigment accumulation in citrus.Unraveling the crosstalk regulatory mechanism between internode elongation and fruit pigmentation provides key genes for breeding of novel types with both dwarf and health-beneficial fortification in citrus.展开更多
基金financially supported by the National Natural Science Foundation of China(22172144)Key Research and Development Program of Zhejiang Province(2023C03148)。
文摘The fabrication of heterojunction catalysts is an effective strategy to enhance charge separation efficiency,thereby boosting the performance of photocatalysts.In this study,BiO_(2-x)nanosheets were synthesized through a hydrothermal process and loaded onto NaNbO_(3) microcube to construct a series of BiO_(2-x)/NaNbO_(3) heterojunctions for photocatalytic N_(2) fixation.Results indicated that 2.5%BiO_(2-x)/NaNbO_(3) had the highest photocatalytic performance.The NH_(3) production rate under simulated solar light reached 406.4μmol·L^(-1)·g^(-1)·h^(-1),which reaches 2.6 and 3.8 times that of NaNbO_(3) and BiO_(2-x),respectively.BiO_(2-x)nanosheets primarily act as electron trappers to enhance the separation efficiency of charge carriers.The strong interaction between BiO_(2-x)and NaNbO_(3) facilitates the electron migration between them.Meanwhile,the abundant oxygen vacancies in BiO_(2-x)nanosheets may facilitate the adsorption and activation of N_(2),which may be another possible reason of the high photocatalytic activity of the BiO_(2-x)/NaNbO_(3).This study may offer new insights for the development of semiconductor materials in photocatalytic nitrogen fixation.
基金supported by the National Natural Science Foundation of China(82022076,81974249,82070136,82104488,and 82305194)the Postdoctoral Science Foundation of China(2023M731222,and 2020T130040ZX)the Foundation of Hubei Key Laboratory of Biological Targeted Therapy(2023swbx021)。
文摘Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion therapy.Although increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutrophils in this process remain unclear.Here,we performed single-cell sequencing of cardiac CD45^(+)cells isolated from the murine myocardium subjected to MIRI at six-time points.We identified diverse types of infiltrating immune cells and their dynamic changes during MIRI.Cardiac neutrophils showed the most immediate response and largest changes and featured with functionally heterogeneous subpopulations,including Ccl3^(hi)Neu and Ym-1^(hi)Neu,which were increased at 6 h and 1 d after reperfusion,respectively.Ym-1^(hi)Neu selectively expressed genes with protective effects and was,therefore,identified as a novel specific type of cardiac cell in the injured heart.Further analysis indicated that neutrophils and their subtypes orchestrated subsequent immune responses in the cardiac tissues,especially instructing the response of macrophages.The abundance of Ym-1^(hi)Neu was closely correlated with the therapeutic efficacy of MIRI when neutrophils were specifically targeted by anti-Lymphocyte antigen 6 complex locus G6D(Ly6G)or anti-Intercellular cell adhesion molecule-1(ICAM-1)neutralizing antibodies.In addition,a neutrophil subtype with the same phenotype as Ym-1^(hi)Neu was detected in clinical samples and correlated with prognosis.Ym-1 inhibition exacerbated myocardial injury,whereas Ym-1 supplementation significantly ameliorated injury in MIRI mice,which was attributed to the tilt of Ym-1 on the polarization of macrophages toward the repair phenotype in myocardial tissue.Overall,our findings reveal the antiinflammatory phenotype of Ym-1^(hi)Neu and highlight its critical role in myocardial protection during the early stages of MIRI.
文摘Sepsis is a lethal condition characterized by multiple organ dysfunction due to disrupted host responses to severe infections.[1]Aff ected patients often have a Sequential Organ Failure Assessment(SOFA)score≥2.[2]Patients with a SOFA score<2 and at least one of the following were considered as“suspected sepsis”:(1)quick SOFA(qSOFA)score≥2;(2)SOFA score=1;or(3)National Early Warning Score(NEWS)4-6.[3]Compared with studies on fluid resuscitation in sepsis patients,there are few studies on fluid management in patients with suspected sepsis.Therefore,we conducted a retrospective cohort study to evaluate the relationship between fluid management and disease progression in suspected sepsis patients.
基金supported by funding from the National Natural Science Foundation of China granted to Q.X.(number 31925034)the National Key Research and Development Program of China granted to Q.X.(number 2022YFF1003100)+3 种基金Major Special Projects and Key R&D Projects in Yunnan Province granted to Q.X.(202102AE090054)the Key Research and Development Program of Hubei granted to X.W.(number 2022BBA155)the Foundation of Hubei Hongshan Laboratory granted to Q.X.(number 2021hszd016)Key Project of Hubei Provincial Natural Science Foundation granted to Q.X.(number 2021CFA017)。
文摘Dwarfing is a pivotal agronomic trait affecting both yield and quality.Citrus species exhibit substantial variation in plant height,among which internode length is a core element.However,the molecular mechanism governing internode elongation remains unclear.Here,we unveiled that the transcriptional cascade consisting of B-BOX DOMAIN PROTEIN 22(BBX22)and ELONGATED HYPOCOTYL 5(HY5)finely tunes plant height and internode elongation in citrus.Loss-of-function mutations of BBX22 in an early-flowering citrus(Citrus hindsii“SJG”)promoted internode elongation and reduced pigment accumulation,whereas ectopic expression of BBX22 in SJG,sweet orange(C.sinensis),pomelo(C.maxima)or heterologous expression of BBX22 in tomato(Solanum lycopersicum)significantly decreased internode length.Furthermore,exogenous application of gibberellin A3(GA3)rescued the shortened internode and dwarf phenotype caused by BBX22overexpression.Additional experiments revealed that BBX22 played a dual role in regulation internode elongation and pigmentation in citrus.On the one hand,it directly bound to and activated the expression of HY5,GA metabolism gene(GA2 OXIDASE 8,GA2ox8),carotenoid biosynthesis gene(PHYTOENE SYNTHASE 1,PSY1)and anthocyanin regulatory gene(Ruby1,a MYB DOMAIN PROTEIN).On the other hand,it acted as a cofactor of HY5,enhancing the ability of HY5 to regulate target genes expression.Together,our results reveal the critical role of the transcriptional cascade consisting of BBX22 and HY5 in controlling internode elongation and pigment accumulation in citrus.Unraveling the crosstalk regulatory mechanism between internode elongation and fruit pigmentation provides key genes for breeding of novel types with both dwarf and health-beneficial fortification in citrus.
基金supported by the National Natural Science Foundation of China (U21A20119)the championship prize funded by Shenzhen Leaguer Co., Ltd.supported in part by the Fundamental Research Funds for the Central Universities。
