SlMC7 prokaryotic expression recombinant protein was used as test materials. SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli. ELISA and pro...SlMC7 prokaryotic expression recombinant protein was used as test materials. SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli. ELISA and protein dot plot methods were used to detect the antibody titer and expression characteristics and the expression difference at different stages,providing material foundation and reference basis for subsequent SlMC7 functional research in tomato fruit. The results showed that the titer of SlMC7 polyclonal antibodies was more than 1∶ 256 000 and the sensitivity of SlMC7 polyclonal antibodies was 1. 6 ng at the dilutability of 1∶ 10 000. SlMC7 protein was detected at different stages,and SlMC7 had a higher expression level in color-break fruit,indicating that SlMC7 might be relevant to the fruit ripening of tomato.展开更多
基于油浸式变压器的顶层油温-绕组等效热路,提出了一种主变压器绕组热点温度的解析模型,该模型能够根据当前采集的主变负载系数和顶层油温数据,实时计算热点温度。相应提出了绕组时间常数的确定方案。通过对某334 MV·A/500 k V主...基于油浸式变压器的顶层油温-绕组等效热路,提出了一种主变压器绕组热点温度的解析模型,该模型能够根据当前采集的主变负载系数和顶层油温数据,实时计算热点温度。相应提出了绕组时间常数的确定方案。通过对某334 MV·A/500 k V主变压器进行实例计算与分析,并将结果与通过GB/T 15164—1994标准中的热点温度计算公式计算的结果进行比较,验证了所提出热点温度模型及计算方法的有效性和正确性,同时指出了GB/T 15164—1994标准中热点温度计算公式存在的缺陷。展开更多
基金Supported by National Natural Science Foundation of China(31672207)
文摘SlMC7 prokaryotic expression recombinant protein was used as test materials. SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli. ELISA and protein dot plot methods were used to detect the antibody titer and expression characteristics and the expression difference at different stages,providing material foundation and reference basis for subsequent SlMC7 functional research in tomato fruit. The results showed that the titer of SlMC7 polyclonal antibodies was more than 1∶ 256 000 and the sensitivity of SlMC7 polyclonal antibodies was 1. 6 ng at the dilutability of 1∶ 10 000. SlMC7 protein was detected at different stages,and SlMC7 had a higher expression level in color-break fruit,indicating that SlMC7 might be relevant to the fruit ripening of tomato.
基金National Natural Science Foundation of China(Grant No.51375247)China Agriculture Research System for Peanut(Grant No.CARS-14-Mechanized Equipment)Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(ASTIP,CAAS)
