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NPS-2143对胶质瘤增殖与迁移能力的影响及机制
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作者 聂佳丽 殷海棠 +7 位作者 文志鹏 郑志昌 李明 李琴 樊兴华 赵青青 张彦燕 杨继红 《贵州医科大学学报》 CAS 2024年第2期157-168,共12页
目的 探究NPS-2143对胶质瘤U87、U251和Hs683细胞增殖与迁移能力的影响及机制。方法 将胶质瘤细胞分为3组,不加药物的对照组(Control组)、6μmol/L和8μmol/L NPS-2143处理组,通过细胞增殖实验CCK-8和细胞平板克隆形成实验检测不同浓度N... 目的 探究NPS-2143对胶质瘤U87、U251和Hs683细胞增殖与迁移能力的影响及机制。方法 将胶质瘤细胞分为3组,不加药物的对照组(Control组)、6μmol/L和8μmol/L NPS-2143处理组,通过细胞增殖实验CCK-8和细胞平板克隆形成实验检测不同浓度NPS-2143对U87和U251细胞活力与增殖的影响,通过流式细胞术实验检测不同浓度NPS-2143对细胞周期和凋亡的影响,Western blot检测细胞凋亡相关蛋白(Bax、Caspase 3)和细胞周期相关蛋白(P21、Cyclin D1)的表达水平;通过细胞划痕实验和细胞迁移实验检测NPS-2143对U87和Hs683细胞迁移的影响,Western blot法检测上皮间质转化(EMT)相关蛋白(N-cadherin、MMP2、MMP9)的表达水平;通过透射电镜观察NPS-2143对U87细胞中自噬囊泡数量的影响,Western blot检测细胞自噬相关蛋白(LC3、P62)和AKT-mTOR信号通路相关蛋白(p-AKT、p-mTOR)的表达水平。结果 与Control组比较,NPS-2143处理组胶质瘤细胞活力与增殖能力降低,且呈时间、浓度依赖性(P<0.01);与Control组比较,NPS-2143处理组胶质瘤细胞G1期细胞比例和凋亡细胞比例升高(P<0.05);与Control组比较,NPS-2143处理组胶质瘤细胞中P21蛋白表达水平增加,Cyclin D1蛋白表达水平下降,细胞凋亡蛋白Bax和Caspase 3表达水平增加(P<0.01);与Control组比较,NPS-2143抑制胶质瘤细胞的迁移能力(P<0.01),胶质瘤细胞中N-cadherin、MMP2、MMP9的表达水平降低(P<0.01),LC3、P62、p-AKT、p-mTOR蛋白表达水平升高(P<0.01);与Control组比较,NPS-2143使胶质瘤细胞内自噬囊泡增加(P<0.01)。结论 NPS-2143能抑制胶质瘤的增殖与迁移,其机制可能与激活AKT-mTOR通路有关。 展开更多
关键词 胶质瘤 NPS-2143 自噬 化疗 上皮间质转化
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Multi-objective Optimal Design of Vibration Absorber with Simple Cell Mapping Algorithm 被引量:1
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作者 Jianqiao Sun Wen Zheng +2 位作者 Furui Xiong zhichang qin Qian Ding 《Transactions of Tianjin University》 EI CAS 2017年第5期488-492,共5页
In this paper, we investigate a multi-objective optimal design of a tuned vibration absorber for a two degrees-of-freedom linear system, in which we take white Gaussian noise as the excitation. We use the simple cell ... In this paper, we investigate a multi-objective optimal design of a tuned vibration absorber for a two degrees-of-freedom linear system, in which we take white Gaussian noise as the excitation. We use the simple cell mapping method to obtain solutions to the multi-objective optimization problem. We optimize tuned vibration absorbers in two different configurations. As our objective, we take various features of the response of the power spectrum density function. Our results show that the primary mass vibration with the optimally designed absorber has a lower peak power spectrum value. In addition, the two natural frequencies of the optimized system are further apart than in the non-optimized system, which implies that the absorber has a wider effective region in the frequency domain. © 2017, Tianjin University and Springer-Verlag GmbH Germany. 展开更多
关键词 Conformal mapping Gaussian noise (electronic) Linear systems MAPPING Optimal systems Optimization Power spectrum
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Influence of Design Reference on Tracking Performance of Feedback Control 被引量:1
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作者 Qiqi Zhao zhichang qin Jianqiao Sun 《Transactions of Tianjin University》 EI CAS 2018年第1期66-72,共7页
In this paper, we present an investigation on the tracking performances of feedback control as a function of reference signals. We use multi-objective optimal designs of feedback controls as a fair basis for comparing... In this paper, we present an investigation on the tracking performances of feedback control as a function of reference signals. We use multi-objective optimal designs of feedback controls as a fair basis for comparing different control designs, and examine step, ramp, and periodic signals at various frequencies. Through comparing the tracking performances of controls designed with different reference signals,we find that the controls designed with ramp signals perform better in tracking step and ramp references than those designed with step signals. To track periodic signals, we find that the controls designed with periodic signals at the same frequency generally provide the best performance, and those designed with step and ramp signals perform comparably. 展开更多
关键词 REFERENCE SIGNAL TRACKING performance FEEDBACK control MULTI-OBJECTIVE optimization
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A multi-objective optimal PID control for a nonlinear system with time delay 被引量:1
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作者 Furui Xiong zhichang qin +6 位作者 Carlos Hernndez Yousef Sardahi Yousef Narajani Wei Liang Yang Xue Oliver Schtze Jianqiao Sun 《Theoretical & Applied Mechanics Letters》 CAS 2013年第6期35-40,共6页
It is generally difficult to design feedback controls of nonlinear systems with time delay to meet time domain specifications such as rise time, overshoot, and tracking error. Furthermore, these time domain specificat... It is generally difficult to design feedback controls of nonlinear systems with time delay to meet time domain specifications such as rise time, overshoot, and tracking error. Furthermore, these time domain specifications tend to be conflicting to each other to make the control design even more challenging. This paper presents a cell mapping method for multi-objective optimal feedback control design in time domain for a nonlinear Duffing system with time delay. We first review the multi-objective optimization problem and its formulation for control design. We then introduce the cell mapping method and a hybrid algorithm for global optimal solutions. Numerical simulations of the PID control are presented to show the features of the multi-objective optimal design. @ 2013 The Chinese Society of Theoretical and Applied Mechanics. [doi:10.1063/2.1306306] 展开更多
关键词 delayed control system multi-objective optimal design cell mapping method hybridalgorithm Pareto optimal
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