目的探讨孕妇年龄、孕周、自然流产次数及胚胎性别与染色体异常的关系。方法应用核型细菌人工染色体标记-微球鉴别/分离(Bacs on Beads, BoBs)技术对济南市妇幼保健院2015年1月至2017年12月共245例自然流产患者的绒毛或死胎组织进行染...目的探讨孕妇年龄、孕周、自然流产次数及胚胎性别与染色体异常的关系。方法应用核型细菌人工染色体标记-微球鉴别/分离(Bacs on Beads, BoBs)技术对济南市妇幼保健院2015年1月至2017年12月共245例自然流产患者的绒毛或死胎组织进行染色体非整倍体检测,分析导致自然流产发生的染色体异常类型。应用χ^2检验对数据进行组间比较。结果(1)245例自然流产或死胎组织均获得核型BoBs检测结果,异常检出率46.1%(113/245),其中常染色体非整倍体占58.4%(66/113),性染色体非整倍体占23.0%(26/113),常染色体部分三体占6.2%(7/113),常染色体部分单体占4.4%(5/113),三倍体占6.2%(7/113),复杂三倍体和双三体各占0.9%(1/113)。(2)≥35岁患者染色体异常比例高于<35岁者[61.0%(36/59)与41.4%(77/186),χ^2=8.003],妊娠早期流产者染色体异常率也高于妊娠中期流产者[48.5%(99/204)与34.2%(14/41),χ^2=4.634],男性胚胎的染色体异常率高于女性胚胎[57.6%(49/85)与40.0%(64/160),χ^2=6.483](P值均<0.05)。不同自然流产史者(0、1和≥2次)相比,染色体异常率差异无统计学意义。结论染色体异常是自然流产的重要因素,染色体非整倍体、部分三体、部分单体和三倍体是导致自然流产或死胎染色体发生异常的主要原因。高龄是导致自然流产或死胎的染色体异常发生率增加的高危因素。展开更多
Regenerating periodontal bone tissues in the aggravated inflammatory periodontal microenvironment under diabetic conditions is a great challenge.Here,a polydopamine-mediated graphene oxide(PGO)and hydroxyapatite nanop...Regenerating periodontal bone tissues in the aggravated inflammatory periodontal microenvironment under diabetic conditions is a great challenge.Here,a polydopamine-mediated graphene oxide(PGO)and hydroxyapatite nanoparticle(PHA)-incorporated conductive alginate/gelatin(AG)scaffold is developed to accelerate periodontal bone regeneration by modulating the diabetic inflammatory microenvironment.PHA confers the scaffold with osteoinductivity and PGO provides a conductive pathway for the scaffold.The conductive scaffold promotes bone regeneration by transferring endogenous electrical signals to cells and activating Ca2+channels.Moreover,the scaffold with polydopamine-mediated nanomaterials has a reactive oxygen species(ROS)-scavenging ability and anti-inflammatory activity.It also exhibits an immunomodulatory ability that suppresses M1 macrophage polarization and activates M2 macrophages to secrete osteogenesis-related cytokines by mediating glycolytic and RhoA/ROCK pathways in macrophages.The scaffold induces excellent bone regeneration in periodontal bone defects of diabetic rats because of the synergistic effects of good conductive,ROS-scavenging,anti-inflammatory,and immunomodulatory abilities.This study provides fundamental insights into the synergistical effects of conductivity,osteoinductivity,and immunomodulatory abilities on bone regeneration and offers a novel strategy to design immunomodulatory biomaterials for treatment of immune-related diseases and tissue regeneration.展开更多
Remaining useful life(RUL)prediction for bearing is a significant part of the maintenance of urban rail transit trains.Bearing RUL is closely linked to the reliability and safety of train running,but the current predi...Remaining useful life(RUL)prediction for bearing is a significant part of the maintenance of urban rail transit trains.Bearing RUL is closely linked to the reliability and safety of train running,but the current prediction accuracy makes it difficult to meet the re-quirements of high reliability operation.Aiming at the problem,a prediction model based on an improved long short-term memory(ILSTM)network is proposed.Firstly,the variational mode decomposition is used to process the signal,the intrinsic mode function with stronger representation ability is determined according to energy entropy and the degradation feature data is constructed com-bined with the time domain characteristics.Then,to improve learning ability,a rectified linear unit(ReLU)is applied to activate a fully connected layer lying after the long short-term memory(LSTM)network,and the hidden state outputs of the layer are weighted by attention mechanism.The Harris Hawks optimization algorithm is introduced to adaptively set the hyperparameters to improve the performance of the LSTM.Finally,the ILSTM is applied to predict bearing RUL.Through experimental cases,the better perfor-mance in bearing RUL prediction and the effectiveness of each improving measures of the model are validated,and its superiority of hyperparameters setting is demonstrated.展开更多
To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study f...To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study found that among patients with clinically node-negative(cN0)breast cancer,97.7%(432/442)with breast pathologic complete response(bpCR)had ypN0(absence of metastases in the axillary lymph nodes);and 71.6%(882/1232)without bpCR achieved ypN0(P<0.001).As for human epidermal growth factor receptor 2(HER2)positive or triple-negative breast cancer(TNBC)that achieved bpCR.展开更多
OBJECTIVE: To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong(CV 17) and Ganshu(BL 18) in rats with hyperplasia of mammary gland(HMG) which induced by estrogen and progestogen.METHODS: ...OBJECTIVE: To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong(CV 17) and Ganshu(BL 18) in rats with hyperplasia of mammary gland(HMG) which induced by estrogen and progestogen.METHODS: Thirty female Sprague-Dawley rats were randomly divided into saline control group,HMG model group, and HMG moxibustion group with 10 in each group. Saline control was the group injected by saline. HMG model were created by injection of estrogen and progestogen. Moxibustion group was also injected of estrogen and progesto-gen with moxibustion at the same time. The Changes of nipple diameter and height were measured.The rats' skin temperature was recorded by an infrared thermal camera at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). Pathological changes of mammary gland in rats were also observed under light microscope.RESULTS: The diameter and height of the nipples in model group were prominently bigger and higher than that in control group(P < 0.01). The diameter and height in moxibustion group were prominently smaller and lower than that in model group(P < 0.01), and there was no significant difference between moxibustion group and control group.Compared with control group, skin temperature of the nipples, mammary area, and acupoints Danzhong(CV 17) and Ganshu(BL 18) decreased prominently in model group(P < 0.01-0.05). Compared with model group, skin temperature of that in moxibustion group increased prominently(P <0.05).CONCLUSION: Treatment with moxibustion can effectively decrease the HMG rats' nipple diameter and height, and increase the skin temperature in HMG model rats at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). This study convinces the therapeutic effect of moxibustion on mammary gland hyperplasia.展开更多
文摘目的探讨孕妇年龄、孕周、自然流产次数及胚胎性别与染色体异常的关系。方法应用核型细菌人工染色体标记-微球鉴别/分离(Bacs on Beads, BoBs)技术对济南市妇幼保健院2015年1月至2017年12月共245例自然流产患者的绒毛或死胎组织进行染色体非整倍体检测,分析导致自然流产发生的染色体异常类型。应用χ^2检验对数据进行组间比较。结果(1)245例自然流产或死胎组织均获得核型BoBs检测结果,异常检出率46.1%(113/245),其中常染色体非整倍体占58.4%(66/113),性染色体非整倍体占23.0%(26/113),常染色体部分三体占6.2%(7/113),常染色体部分单体占4.4%(5/113),三倍体占6.2%(7/113),复杂三倍体和双三体各占0.9%(1/113)。(2)≥35岁患者染色体异常比例高于<35岁者[61.0%(36/59)与41.4%(77/186),χ^2=8.003],妊娠早期流产者染色体异常率也高于妊娠中期流产者[48.5%(99/204)与34.2%(14/41),χ^2=4.634],男性胚胎的染色体异常率高于女性胚胎[57.6%(49/85)与40.0%(64/160),χ^2=6.483](P值均<0.05)。不同自然流产史者(0、1和≥2次)相比,染色体异常率差异无统计学意义。结论染色体异常是自然流产的重要因素,染色体非整倍体、部分三体、部分单体和三倍体是导致自然流产或死胎染色体发生异常的主要原因。高龄是导致自然流产或死胎的染色体异常发生率增加的高危因素。
基金grants from Sichuan Key Research and Development Program of China(22ZDYF2034)National Natural Science Foundation of China(grant no.82072071,82072073)+5 种基金Key-Area Research and Development Program of Guang Dong Province(2019B010941002)Shenzhen Funds of the Central Government to Guide Local Scientific and Technological Development(2021SZVUP123)Sichuan Science and Technology Program(2020YFS0170)Fundamental Research Funds for the Central Universities(2682020ZT79)Guangdong Basic and Applied Basic Research Foundation(2021B1515120019)Research and Develop Program,West China Hospital of Stomatology Sichuan University(RD-03-202012,RD-03-202101).
文摘Regenerating periodontal bone tissues in the aggravated inflammatory periodontal microenvironment under diabetic conditions is a great challenge.Here,a polydopamine-mediated graphene oxide(PGO)and hydroxyapatite nanoparticle(PHA)-incorporated conductive alginate/gelatin(AG)scaffold is developed to accelerate periodontal bone regeneration by modulating the diabetic inflammatory microenvironment.PHA confers the scaffold with osteoinductivity and PGO provides a conductive pathway for the scaffold.The conductive scaffold promotes bone regeneration by transferring endogenous electrical signals to cells and activating Ca2+channels.Moreover,the scaffold with polydopamine-mediated nanomaterials has a reactive oxygen species(ROS)-scavenging ability and anti-inflammatory activity.It also exhibits an immunomodulatory ability that suppresses M1 macrophage polarization and activates M2 macrophages to secrete osteogenesis-related cytokines by mediating glycolytic and RhoA/ROCK pathways in macrophages.The scaffold induces excellent bone regeneration in periodontal bone defects of diabetic rats because of the synergistic effects of good conductive,ROS-scavenging,anti-inflammatory,and immunomodulatory abilities.This study provides fundamental insights into the synergistical effects of conductivity,osteoinductivity,and immunomodulatory abilities on bone regeneration and offers a novel strategy to design immunomodulatory biomaterials for treatment of immune-related diseases and tissue regeneration.
基金supported by the National Natural Science Foundation of China(Grant No.U22A2053)Major Science and Technology Project of Guangxi Province of China(Grant No.Guike AB23075209)+1 种基金Guangxi Manufacturing Systems and Advanced Manufacturing Technology Key Laboratory Director Fund(Grant No.21-050-44-S015)Innovation Project of Guangxi Graduate Education(Grant No.YCSW2023086).
文摘Remaining useful life(RUL)prediction for bearing is a significant part of the maintenance of urban rail transit trains.Bearing RUL is closely linked to the reliability and safety of train running,but the current prediction accuracy makes it difficult to meet the re-quirements of high reliability operation.Aiming at the problem,a prediction model based on an improved long short-term memory(ILSTM)network is proposed.Firstly,the variational mode decomposition is used to process the signal,the intrinsic mode function with stronger representation ability is determined according to energy entropy and the degradation feature data is constructed com-bined with the time domain characteristics.Then,to improve learning ability,a rectified linear unit(ReLU)is applied to activate a fully connected layer lying after the long short-term memory(LSTM)network,and the hidden state outputs of the layer are weighted by attention mechanism.The Harris Hawks optimization algorithm is introduced to adaptively set the hyperparameters to improve the performance of the LSTM.Finally,the ILSTM is applied to predict bearing RUL.Through experimental cases,the better perfor-mance in bearing RUL prediction and the effectiveness of each improving measures of the model are validated,and its superiority of hyperparameters setting is demonstrated.
基金Beijing Medical Reward Foundation(No.YXJL-2016-0040-0012)
文摘To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study found that among patients with clinically node-negative(cN0)breast cancer,97.7%(432/442)with breast pathologic complete response(bpCR)had ypN0(absence of metastases in the axillary lymph nodes);and 71.6%(882/1232)without bpCR achieved ypN0(P<0.001).As for human epidermal growth factor receptor 2(HER2)positive or triple-negative breast cancer(TNBC)that achieved bpCR.
基金Supported by Grants of Beijing Key Laboratory of Clothing Material R&D and Assessment(No.2013ZK-05)Beijing Municipal Commission of Education(No.KM201510012003)
文摘OBJECTIVE: To evaluate the efficacy of moxibustion, through stimulating acupoints of Danzhong(CV 17) and Ganshu(BL 18) in rats with hyperplasia of mammary gland(HMG) which induced by estrogen and progestogen.METHODS: Thirty female Sprague-Dawley rats were randomly divided into saline control group,HMG model group, and HMG moxibustion group with 10 in each group. Saline control was the group injected by saline. HMG model were created by injection of estrogen and progestogen. Moxibustion group was also injected of estrogen and progesto-gen with moxibustion at the same time. The Changes of nipple diameter and height were measured.The rats' skin temperature was recorded by an infrared thermal camera at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). Pathological changes of mammary gland in rats were also observed under light microscope.RESULTS: The diameter and height of the nipples in model group were prominently bigger and higher than that in control group(P < 0.01). The diameter and height in moxibustion group were prominently smaller and lower than that in model group(P < 0.01), and there was no significant difference between moxibustion group and control group.Compared with control group, skin temperature of the nipples, mammary area, and acupoints Danzhong(CV 17) and Ganshu(BL 18) decreased prominently in model group(P < 0.01-0.05). Compared with model group, skin temperature of that in moxibustion group increased prominently(P <0.05).CONCLUSION: Treatment with moxibustion can effectively decrease the HMG rats' nipple diameter and height, and increase the skin temperature in HMG model rats at the nipples, mammary areas,Danzhong(CV 17) and Ganshu(BL 18). This study convinces the therapeutic effect of moxibustion on mammary gland hyperplasia.
