During 2015, marginal scorch symptoms were detected in the production base of indigowoad rootin Hubei, China. On the basis of morphological features and 18S rDNA sequences, the pathogen was identified as Cladosporium ...During 2015, marginal scorch symptoms were detected in the production base of indigowoad rootin Hubei, China. On the basis of morphological features and 18S rDNA sequences, the pathogen was identified as Cladosporium sp. Koch’s postulates were fulfilled by pathogenicity tests on potted indigowoad root seedlings. To our knowledge, this report is the first of marginal scorch on indigowoad root caused by Cladosporium sp. We propose the name “marginal scorch” for the new disease.展开更多
Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) whic...Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) which is generated by Bacillus species has inhibitory effect on soft rot pathogen through disrupting the signal molecules (N-acylhomoserine lactones, AHL) of their Quorum Sensing system. The aim of our study is to obtain recombinant yeast which produces AiiA protein. The recombinant yeast Pichia pastoris GS115 was constructed to constitutive expression of the AiiA gene. The results of reverse transcript PCR analysis showed that the AiiA gene was expressed successfully in the yeast. Proteins extracted from YPDS showed the highest inhibition efficacy to E. carotovora compared with the other two mediums (YPD and LB) under tested conditions.展开更多
文摘During 2015, marginal scorch symptoms were detected in the production base of indigowoad rootin Hubei, China. On the basis of morphological features and 18S rDNA sequences, the pathogen was identified as Cladosporium sp. Koch’s postulates were fulfilled by pathogenicity tests on potted indigowoad root seedlings. To our knowledge, this report is the first of marginal scorch on indigowoad root caused by Cladosporium sp. We propose the name “marginal scorch” for the new disease.
文摘Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) which is generated by Bacillus species has inhibitory effect on soft rot pathogen through disrupting the signal molecules (N-acylhomoserine lactones, AHL) of their Quorum Sensing system. The aim of our study is to obtain recombinant yeast which produces AiiA protein. The recombinant yeast Pichia pastoris GS115 was constructed to constitutive expression of the AiiA gene. The results of reverse transcript PCR analysis showed that the AiiA gene was expressed successfully in the yeast. Proteins extracted from YPDS showed the highest inhibition efficacy to E. carotovora compared with the other two mediums (YPD and LB) under tested conditions.
